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All Journal Indonesian Journal of Cancer Chemoprevention Journal of Mathematical and Fundamental Sciences Indonesian Journal of Biotechnology Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology INDONESIAN JOURNAL OF PHARMACY The Indonesian Biomedical Journal JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) JPSCR : Journal of Pharmaceutical Science and Clinical Research JFIOnline Jurnal Ilmu Kefarmasian Indonesia Majalah Kesehatan Pharmamedika
Muthi Ikawati
Faculty Of Pharmacy, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Journal : Majalah Obat Tradisional

 1 
POTENSI EKSTRAK ETANOLIK KULIT BUAH JERUK NIPIS (Citrus aurantiifolia (Cristm.) Swingle) SEBAGAI AGEN KHEMOPREVENTIF MELALUI PENEKANAN EKSPRESI c-Myc DAN PENGHAMBATAN PROLIFERASI PADA SEL PAYUDARA TIKUS GALUR SPRAGUE DAWLEY TERINDUKSI 7,12-DIMETILBENZ[a]ANTRASENA Meiyanto, Edy; Pratiwi, Dewi; Hastuti, Novi; Nur W, Niken; Armandari, Inna; Ikawati, Muthi’; Hermawan, Adam
Majalah Obat Tradisional Vol 15, No 1 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.754 KB) | DOI: 10.14499/mot-TradMedJ15iss1pp%p

Abstract

POTENSI  EKSTRAK ETANOLIK KULIT BUAH JERUK NIPIS (Citrus aurantiifolia (Cristm.) Swingle) SEBAGAI AGEN KHEMOPREVENTIF MELALUI PENEKANAN EKSPRESI c-Myc DAN PENGHAMBATAN PROLIFERASI PADA SEL PAYUDARA TIKUS GALUR SPRAGUE DAWLEY TERINDUKSI 7,12-DIMETILBENZ[a]ANTRASENA POTENCY OF CITRUS PEELS (Citrus aurantiifolia (Cristm.) Swingle) ETHANOLIC EXTRACT AS CHEMOPREVENTIVE  AGENT THROUGH DOWNREGULATION OF           c-Myc EXPRESSION AND INHIBITION OF 7.12-DIMETHYLBENZ[a]ANTRACHENE INDUCED FEMALE  SPRAGUE DAWLEY RATS BREAST CELL PROLIFERATION Dewi Pratiwi, Novi Hastuti, Niken Nur W, Inna Armandari, Muthi’ Ikawati,Adam Hermawan  dan Edy Meiyanto*)Cancer Chemoprevention Research Center Fakultas Farmasi, Universitas Gadjah Mada ABSTRAK Penggunaan obat berbasis alam saat ini berkembang pesat di semua kalangan masyarakat. Selain karena harga yang lebih terjangkau, obat berbasis alam relatif lebih aman dibandingkan dengan obat sintetik. Kulit jeruk nipis (Citrus aurantiifolia) merupakan salah satu obat berbasis alam mengandung flavonoid yang berpotensi sebagai antikarsinogenesis.  Penelitian ini dirancang untuk mengkaji potensi kulit jeruk nipis (C. aurantiifolia) dalam menekan proliferasi sel  payudara tikus galur Sprague Dawley yang terinduksi 7,12-Dimetilbenz[a]Antrasena (DMBA).  Dalam penelitian ini, tikus dibagi menjadi lima kelompok yakni kelompok perlakuan DMBA, kelompok perlakuan CMC-Na, kelompok perlakuan ekstrak dosis 1500 mg/kgBB , kelompok perlakuan DMBA+ekstrak dosis 750 mg/kgBB dan perlakuan DMBA+ekstrak dosis 1500 mg/kgBB. Pengamatan proliferasi sel payudara dengan metode AgNOR menunjukkan bahwa pemberian ekstrak kulit C. aurantiifolia dapat menekan proliferasi sel secara signifikan. Secara kuantitatif signifikansi yang dihasilkan dosis  1500 mg/kgBB lebih tinggi daripada dosis 750 mg/kgBB. Hasil pengamatan imunohistokimia pada ekspresi c-Myc mendukung data sebelumnya. Pada kelompok dosis 750 terlihat warna coklat pada sitosol yang lebih intens dibanding kelompok dosis 1500. Ekstrak etanolik kulit jeruk nipis dapat menekan proliferasi sel payudara terinduksi DMBA, penekanan proliferasi tersebut meningkat seiring peningkatan dosis sehingga jeruk nipis dapat digunakan sebagai agen khemopreventif.
POTENSI EKSTRAK ETANOLIK KULIT BUAH JERUK NIPIS (Citrus aurantiifolia (Cristm.) Swingle) SEBAGAI AGEN KHEMOPREVENTIF MELALUI PENEKANAN EKSPRESI c-Myc DAN PENGHAMBATAN PROLIFERASI PADA SEL PAYUDARA TIKUS GALUR SPRAGUE DAWLEY TERINDUKSI 7,12-DIMETILBENZ[a]ANTRASENA Dewi Pratiwi; Novi Hastuti; Niken Nur W; Inna Armandari; Muthi’ Ikawati; Adam Hermawan; Edy Meiyanto
Majalah Obat Tradisional Vol 15, No 1 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.754 KB) | DOI: 10.22146/tradmedj.8063

Abstract

The using of natural-based medicine is growing rapidly in societies. Besides being cheap and affordable, natural-based medicine is relatively safer than the synthetic drugs. Peel of Citrus aurantifolia (Cristm.) Swingle) is one of the chemopreventive agent which contain flavonoids have potency as anticarcinogenic agent. This study is designed to study the potency of Citrus aurantifolia peel ethanolic extract in proliferation inhibition of Rattus norvegicus mammary cell of Sprague Dawley strain which is induced by 7,12-Dimethylbenz[a]anthracene (DMBA). Rats were divided into five groups consist of DMBA treatment, CMC-Na treatment, extract 1500 mg/kgBW treatment, treatment of DMBA+ extract 750 mg/kgBW and DMBA+ extract 1500 mg/kgBW. At the beginning of the tenth week of the study, breasts was isolated and stored in 10% formalin buffer. Observation of cell proliferation was done by AgNOR method. C-Myc expression observed using immunohistochemistry (IHC). Observation of mammary cell with AgNOR method indicated that the treatment of Citrus aurantifolia peel ethanolic extract can inhibit cell proliferation significantly. Dosage 1500 mg/kgBW gave higher inhibition effect than dosage 750 mg/kgBW. IHC result showed that treatment of Citrus aurantifolia peel ethanolic extract decrease the expression of c-Myc. Dosage 750 mg/kgBW gave lower decreasing effect than dosage 1500 mg/kgBW. Citrus aurantifolia peel ethanolic extract inhibited the proliferation of mammary cell induced DMBA through the inhibition of c-Myc expression in dose dependent phenomena so that it is a potential chemopreventive agent.
Co-Authors . Anindyajati Abdul Manaf Ali, Abdul Manaf Adam Hermawan ADTYA FITRIASARI Afifah, Anis Afivah Dewi Anggraeni Agusta Fauzi, Ilham Alfi Yasmina Angelina, Dhella Anggoro, Bayu ANINDYAJATI ANINDYAJATI Anindyajati Anindyajati ANNISA KARAMITA Annisa Khumaira Arief Nurrochmad Astrid Ayu Maruti Astrid Ayu Maruti Chio Oka, Chio Dewi Pratiwi Dewi Pratiwi Dhania Novitasari Dhiya Ulhaq Salsabila Dyaningtyas D.P. Putri Dyaningtyas Dewi Putri Pamungkas EDIATI EDIATI Ediati Sasmito Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto EDY MEIYANTO Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Een Sri Endah Endah Puji Septisetyani Endah Puji Septisetyani, Endah Puji Erlina Rivanti Erlina Rivanti Febri Wulandari Fikri Amalia Gono, Christina Mutiara Putri Hanifa, Mila Hapsari, Novia Permata Hilyatul Fadliyah Ibrahim Arifin Ika Puspitaningrum Ika Putri Nurhayati Ilham Augusta F Ilham Augusta F. Imaniyyati, Niar Nurul Inna Armandari Inna Armandari Jenie, Riris Istighfari Jun-Ya Kato Jun-ya Kato Kumara, Dennaya Mintarsih, Betty Mintarsih, Betty Mitsunori Kirihata Moordiani Moordiani Muhammad Novrizal Abdi Sahid Mustofa Mustofa Nanang Fakhrudin, Nanang Nanda Resa Pratama Nanda Resa Pratama Natasia, Nyssa Niken Nur W Niken Nur W, Niken Nindya Budiana Putri Normaidah, Normaidah Novi Hastuti Novi Hastuti, Novi Nurma Sabila Nurramadhani A. Sida Purwanto, Heri Putri, Amaliya Permata Rahman, Faaza Aulia Rahmani, Mawardi Rahmani, Mawardi Rahmawati, Desty Restia Ratih Hardika Pratama RATIH HARDIKA PRATAMA Ratih Hardika Pratama Ratna Asmah Susidarti Ratna Asmah Susidarti Ratna Asmah Susidarti Rhamandana, I Made Rifai, Fauziah Novita Putri RIRIS ISTIGHFARI JENIE Rita Riata Rita Riata Ritmaleni, Ritmaleni Rohmad Yudi Utomo Sagiyo, Marrita Langgeng Sari Haryanti Sari Haryanti Shirly Kumala Sismindari, Sismindari Sri Handayani Sri Kasianningsih Sri Kasianningsih Sri Pudjiraharti Sukari, Mohd. Aspollah Sukari, Mohd. Aspollah Susi Ari Kristina Susi Ari Kristina Susi Ari Kristina Syifa Athia Zainun Faqiha Tafrihani, Ahmad Syauqy Wardani, Ratih Kurnia Wulandari Wulandari Yogi Ertanto Yogi Ertanto Yohanes, Jasson Yurista Gilang Yurista Gilang YURISTA GILANG IKHTIARSYAH Ziana Walidah Zulfin, Ummi Maryam