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All Journal VALENSI Jurnal Penelitian Saintek SAINS DAN MATEMATIKA JURNAL KIMIA SAINS DAN APLIKASI Alchemy Jurnal Penelitian Kimia INDONESIAN JOURNAL OF PHARMACY Jurnal Bina Praja Indonesian Journal of Chemistry Mendapo: Journal of Administrative Law Current Research on Biosciences and Biotechnology Jurnal Info Sains : Informatika dan Sains Greensphere: Journal of Environmental Chemistry
Ismiyarto Ismitarto
Department Of Chemistry, Faculty Of Science And Mathematics, Diponegoro University Jl. Prof. H. Soedarto SH Tembalang, Semarang 50275, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Energy Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences

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Journal : Jurnal Penelitian Saintek

 1 
AKTIVITAS ANTIDIABETES METABOLIT SEKUNDER BAKTERI ENDOFIT ASAL KULIT KAYU MANIS Purbowatiningrum Ria Sarjono; Hendra Dwipa Rifky Mahardika; Nies S. Mulyani; Ngadiwiyana Ngadiwiyana; Nor Basid Adi Prasetyawibowo; Ismiyarto Ismiyarto
Jurnal Penelitian Saintek Vol 25, No 2 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v25i2.32892

Abstract

Penelitian ini bertujuan untuk mengisolasi bakteri endofit yang bersimbiosis dengan kulit kayu manis, mendapatkan data kemampuan inhibisi enzim α-glukosidase dari metabolit sekunder isolat bakteri endofit yang didapatkan, serta mendapatkan informasi mengenai kandungan kimia dari metabolit sekunder isolat bakteri endofit yang didapat. Bahan yang digunakan pada penelitian ini terdiri atas sampel kulit kayu manis (Cinnamomum burmanii) yang didapatkan dari daerah Kopeng, Kabupaten Semarang. Metode yang digunakan dalam sterilisasi adalah metode klorin. Pengamatan morfologi koloni dilakukan dengan pewarnaan gram sedangkan Uji Inhibisi α-glukosidase dari metabolit menggunakan metode dari Sancheti. Aktivitas antidiabetes diuji menggunakan metode penghambatan enzim α-glukosidase. Hasil yang diperoleh pada penelitian ini adalah lima isolat bakteri memiliki yang bentuk beragam yakni streptobacillus (isolat C1, isolat C4, dan isolat C5), diplobacillus (isolat C2), dan diplococcus (isolat C3). Kemampuan inhibisi α-glukosidase tertinggi dihasilkan oleh metabolit sekunder isolat C5 yakni sebesar 45,634% pada konsentrasi 100 ppm. Penapisan fitokimia pada penelitian ini menunjukkan bahwa metabolit sekunder isolat C5 yang memiliki daya inhibisi tertinggi mengandung senyawa flavonoid, tanin, kuinon dan saponin.ANTIDIABETIC ACTIVITY FROM SECONDARY METABOLIM ENDOPHYTIC BACTERIA BARKThis study was aimed at isolating endophytic bacteria symbiotic with cinnamon bark, obtaining data on the inhibition ability of α-glucosidase enzymes from secondary metabolites of endophytic bacterial isolates obtained, and obtaining information on the chemical content of secondary metabolites of endophytic bacterial isolates obtained. The materials used in this study consisted of the samples of cinnamon bark (Cinnamomum burmanii) obtained from Kopeng, Semarang. The method used in sterilization was the chlorine method. Colony morphological observations were carried out by using gram staining, while the α-glucosidase inhibition test of metabolites used the Sancheti method. The antidiabetic activity was tested using the α-glucosidase enzyme inhibition method. The results show that five bacterial isolates had various forms, namely streptobacillus (isolate C1, isolate C4, and isolate C5), diplobacillus (isolate C2), and diplococcus (isolate C3). The highest α-glucosidase inhibition ability was produced by secondary metabolites of isolate C5, namely 45.634% at a concentration of 100 ppm. Phytochemical screening in this study showed that the secondary metabolites of isolate C5 which had the highest inhibitory power contained flavonoids, tannins, quinones and saponins.
MODIFIKASI KAIN AKTIF ANTIBAKTERI BERBASIS KOMPLEK Mn(II) BASA SCHIFF KITOSAN–SALISILALDEHID Ismiyarto - Ismiyarto; Sesika Novari; Ngadiwiyana Ngadiwiyana; Purbowatiningrum Ria Sarjono; Noor Basid Adiwibawa Prasetya
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1119.408 KB) | DOI: 10.21831/jps.v25i1.29821

Abstract

Penelitian ini bertujuan untuk memperoleh kompleks Mn(II)-basa Schiff kitosan-salisaldehida dan menguji aktivitasnya sebagai bahan aktif kain antibakteri. Metode penelitian dilakukan melalui tiga tahapan. Pada tahap pertama dilakukan sintesis basa Schiff kitosan–salisilaldehid dengan mereaksikan kitosan dengan variasi mol salisilaldehid menggunakan campuran pelarut asam asetat dan etanol, diaduk pada suhu 50oC selama 6 jam. Pada tahap kedua, basa Schiff kitosan–salisilaldehid dijadikan kompleks dengan MnCl2.2H2O menggunakan campuran pelarut asam asetat 2% dan etanol, diaduk pada suhu 60oC selama 12 jam. Pada tahap ketiga dilakukan pelapisan kompleks pada kain katun menggunakan metode spray dan selanjutnya dilakukan uji antibakteri terhadap E. coli (G-) dan S. aureus (G+) menggunakan metode difusi cakram. Hasil penelitian menunjukkan bahwa basa Schiff kitosan–salisilaldehid dengan rasio substitusi paling besar (0,122) diperoleh ketika disintesis menggunakan salisilaldehid 0,00227 mol. Senyawa kompleks Mn(II)-basa Schiff kitosan–salisilaldehid yang terbentuk berupa serbuk berwarna hitam dengan rendemen 66%. Kain katun yang telah dilapisi kompleks Mn(II)-basa Schiff kitosan–salisilaldehid efektif menghambat pertumbuhan bakteri dengan persen daya hambat sebesar 16,02% terhadap bakteri E. coli (G-) dan 14,02% terhadap bakteri S. aureus (G+).MODIFICATION OF ANTIBACTERIAL FABRIC FROM Mn(II) SCHIFF BASE CHITOSAN - SALICYLALDEHYDE COMPLEXThis study was aimed at obtaining the complex of Mn(II)-Schiff base chitosan- salicylaldehyde and evaluating the activity of antibacterial agent on cotton fabric. The study was conducted through three treatment steps. In the first step, chitosan-salicylaldehyde Schiff base was synthesized by reacting chitosan with mole variations of salicylaldehyde and combined with a mixture of acetic acid and ethanol as the solvent which was then stirred at 50oC for 6 hours. In the second step, preparation complexes Mn(II)- Schiff base chitosan-salicylaldehyde was conducted by mixing chitosan-salicylaldehyde Schiff base and MnCl2.2H2O using acetic acid 2% -ethanol as a solvent at 60oC for 12 h. In the third step, the complex was coated onto fabric using spray method and the E. coli (G-) dan S. aureus (G+) antibacterial test was done to the coated fabric using disk diffusion method. The results showed that 0.00227 mole salicylaldehyde lead to the highest substitution ratio of Schiff base chitosan-salicylaldehyde of 0.122. The Mn(II)-Schiff base chitosan-salicylaldehyde complex was obtained as black powder with a yield of 66%. Cotton fabric that has been coated with the complex, effectively inhibits bacterial growth with a percent inhibition against E. coli (G-) and S. aureus (G+) bacteria of 16.02% and 14.02%, respectively. 
Penentuan total fenolik dan flavonoid serta uji aktivitas antioksidan dari daun mangga kultivar madu Fachriyah, Enny; Wati, Mei Riska; ismiyarto, Ismiyarto; Sarjono, Purbowatiningrum Ria
Jurnal Penelitian Saintek Vol 29, No 2 (2024)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i2.77028

Abstract

Penelitian ini bertujuan untuk melakukan uji fitokimia yang meliputi uji alkaloid, flavonoid, saponin, fenolik, kuinon, triterpenoid dan steroid, total fenolik dan flavonoid serta aktivitas antioksidan fraksi etil asetat dan etanol daun mangga kultivar madu. Penelitian diawali determinasi tanaman, preparasi sampel, skrining fitokimia, maserasi dengan etanol 96% dilanjutkan partisi bertingkat menggunakan n-heksana dan etil asetat. Skrining fitokimia dilakukan pada ekstrak dan fraksi yang diperoleh. Fraksi yang diperoleh ditentukan total fenolik dengan metode Folin-Ciocalteu, total fl avonoid dengan metode kolorimetri AlCl3. Aktivitas antioksidan ditentukan dengan metode DPPH. Hasil determinasi tanaman dapat dipastikan sampel adalah Mangifera indica L. kultivar madu, positif mengandung senyawa flavonoid, fenolik, saponin, kuinon, steroid dan triterpenoid. Rendemen fraksi etil asetat dan fraksi etanol berturut-turut sebesar 6,19 dan 5,19%. Kadar total fenolik fraksi etil asetat daun mangga kultivar madu berturut-turut sebesar 423,7 ± 3,68 mg GAE/g dan 48,2 ± 0,139 mg QE/g. Kadar total flavonoid fraksi etanol berturut-turut sebesar 132,35 ± 1,95 mg GAE/g dan 27,72 mg QE/g. Nilai IC50 fraksi etil asetat dan fraksi etanol berturut-turut sebesar 17,83 dan 79,73 ppm. Daun mangga kultivar madu dapat berperan sebagai agen antioksidan dengan kategori kuat pada fraksi etanol dan sangat kuat pada fraksi etil asetat.Kata kunci: total fenolik, total flavonoid, antioksidan, Mangifera indica LDetermination of total phenolics and flavonoids content and test of antioxidant activity of mango leaves honey cultivar Abstract: This research aims to carry out phytochemical tests which include tests for alkaloids, flavonoids, saponins, phenolics, quinones, saponins, triterpenoids and steroids, total phenolics and fl avonoids as well as antioxidant activity of the ethyl acetate and ethanol fractions of honey cultivar mango leaves. The research began with plant determination, sample preparation, phytochemical screening, maceration with 96% ethanol followed by multilevel partitioning using n-hexane and ethyl acetate. Phytochemical screening was carried out on the extracts and fractions obtained. The fractions obtained were determined as total phenolics using the Folin Ciocalteu method, total flavonoids using the AlCl3 colorimetric method. Antioxidant activity was determined by the DPPH method. The results of the plant determination confi rmed that the sample was Mangifera indica L. honey cultivar, positive for containing flavonoid, phenolic, saponin, quinone, steroid and triterpenoid compounds. The yields of the ethyl acetate fraction and ethanol fraction were 6.19 and 5.19% respectively. The total phenolic content of the ethyl acetate fraction of honey cultivar mango leaves was respectively 423.7 ± 3.68 mg GAE/g and 48.2 ± 0.139 mg QE/g. The total flavonoid content of the ethanol fraction was 132.35 ± 1.95 mg GAE/g and 27.72 mg QE/g, respectively. The IC50 values of the ethyl acetate fraction and ethanol fraction were 17.83 and 79.73 ppm respectively. Honey cultivar mango leaves can act as an antioxidant agent with a strong category in the ethanol fraction and very strong in the ethyl acetate fraction. Keywords: total phenolics content, total flavonoids content, antioxidant, Mangifera indica L
Antioxidant and antibacterial activities of alkaloids from endophytic bacterium Pseudomonas hibiscicola Wk isolated from waru (Hibiscus tiliaceus) leaves Sarjono, Purbowatiningrum Ria; Laksmitasari, Nuraini Dwi; Asy'ari, Mukhammad; Ngadiwiyana, Ngadiwiyana; Ismiyarto, Ismiyarto; Fachriyah, Enny
Jurnal Penelitian Saintek Vol 30, No 1 (2025)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v30i1.77501

Abstract

This study aims to investigate the antioxidant and antibacterial activity from alkaloid extract. Alkaloid compounds were isolated from the endophytic bacteria Pseudomonas hibiscicola Wk, which was obtained from Hibiscus leaves. The alkaloid compounds were characterized using thin layer chromatography. The isolated alkaloid compounds were tested for total phenol content, and antioxidant activity. The phytochemical analysis of the alkaloid compounds from the endophytic bacteria Pseudomonas hibiscicola Wk showed a positive result for alkaloids, confirmed by an Rf value of 0.8125 which is similar to the Rf value of alkaloids from the periwinkle plant, namely vindoline (Rf = 0.85). Alkaloid compounds from the endophytic bacteria Pseudomonas hibiscicola Wk had the highest total phenol content of 2.390 mg gallic acid/gram sample at a concentration of 10.000 mg/L. These results indicate that the alkaloid compounds obtained contain phenol groups. The IC50 value obtained from the isolated alkaloid compound was 99.70 mg/L, demonstrating strong antioxidant capacity, and has the ability to inhibit the growth of E.coli and S.aureus bacterial.
Co-Authors A.A. Ketut Agung Cahyawan W Adityo Anggadita Agus Subagio Ahmad Fathoni Alik Kandhita Febriani Anjar Jatika Anshori, Yuli Tirtariandi El Ardhita Niken Destiana Asy'ari, Mukhammad Asy’ari, Mukhammad Ayu Ratri Kartika Iriany Bambang Cahyono Bayu Refindra Fitriadi Chairil Anwar Chairil Anwar Choirunnisa, Nur Fadilla Damar Nurwahyu Bima Desy Fitrya Syamsumir Didik Setiyo Widodo Dimas Syamsudin Ditya Putri Solihati Dzahabiyyah, Hana Putri Enceng Enceng Enny Fachriyah Enny Fachriyah Fannie Kurniasari Fithri Noorikhlas Halim, Siti Anisah Hanum, Rahma Farida Hendra Dwipa Rifky Mahardika Hermansyah Amir Huda, Muhammad Badrul Intan Endah Rachmawati Jaluanto Sunu Punjul Tyoso Jumina Jumina Khairul Anami Laksmitasari, Nuraini Dwi Leong Kai Vyn Liswinda Zafirah Rahmatia Madiyono, Madiyono Marcelinus Christwardana Meiny Suzery Mumtazati, Qonita Murni Nur Islamiah Kassim Ngadiwiyana M.Si. S.Si. Nies S. Mulyani Niken Windi Saputri Ningsih, Alyxia Stellata Aria Noor Basid Adiwibawa Prasetya Nor Basid Adiwibawa Prasetya Nor Basid Adiwibawa Prasetya Nur Amaliyah Nurlina Priastuti Pandelaki, Elmi Christi Julia Pratama Jujur Wibawa Purbowatiningrum Ria Sarjono Purwaningdyah, Purwaningdyah Rani Mustika Ratnawati Dwi Putranti Honorata Rinaryadi, Kemilau Permata Hati Sabirin Matsjeh Salsabila, Salsabila Sesika Novari Suradi, Agus Suyanti Suyanti Triwijayanti, Yunita Wati, Mei Riska Yosie Andriani Yosie Andriani