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All Journal Jurnal Sain Veteriner
Faisal Jamin
Universitas Syiah Kuala
Veterinary

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Aktivitas Ekstrak Etanol Daun Bandotan Terhadap Pertumbuhan Bakteri Vibrio sp. Isolat Ikan Lele Dumbo (Clarias gariepinus) Admi, Masda; Fitri, Khairani; Hennivanda, Hennivanda; Jamin, Faisal; Rosmaidar, Rosmaidar; Syafruddin, Syafruddin; Winaruddin, Winaruddin; Azhari, Azhari
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.96568

Abstract

     Daun bandotan merupakan salah satu tanaman obat yang banyak dimanfaatkan  dalam mengobati berbagai jenis penyakit karena mengandung senyawa fitokimia yang dapat menghambat pertumbuhan bakteri seperti alkaloid, flavonoid, tanin, saponin dan steroid. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol daun bandotan terhadap pertumbuhan bakteri Vibrio sp. isolat ikan lele dumbo. Pelaksanaan penelitian ini menggunakan metode Kirby Bauer disk diffusion yang diawali dengan peremajaan stok isolat bakteri pada media Nutrient Broth, reidentifikasi dengan media Thiosulfate Citrate Bile Salt Sucrose Agar dan pewarnaan Gram. Selanjutnya dilakukan uji daya hambat menggunakan media Mueller Hinton Agar. Studi ini terdiri dari 5 perlakuan yaitu ekstrak etanol daun bandotan konsentrasi 25% (P1), 50% (P2), dan 75% (P3), siprofloksasin sebagai kontrol positif dan CMC 1% sebagai kontrol negatif. Hasil penelitian menunjukkan diameter zona hambat yang terbentuk pada kontrol positif (45,13 ± 0,04mm) dua kali lebih besar dibandingkan daya hambat yang terbentuk pada ekstrak daun bandotan konsentrasi 25% (23,30 ± 0,15mm), konsentrasi 50% (24,29 ± 0,17 mm), dan konsentrasi 75% (25,29 ± 0,16mm), kontrol negatif tidak menunjukkan terbentuknya zona hambat. Kesimpulan penelitian adalah ekstrak etanol daun bandotan berpotensi dapat menghambat pertumbuhan bakteri Vibrio sp. hasil isolat lele dumbo.      
Isolation of Aspergillus sp. from The Lungs of Sparrows (Passer montanus) Nugraha, Ronaldi Fajar; Erina, Erina; AK, M. Daud; Jamin, Faisal; Darniati, Darniati
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.102705

Abstract

Aspergillosis is a common respiratory disease in avian species. The infection of Aspergillus sp. is characterized by the presence of yellowish plaques or nodules in the respiratory tract such as the trachea, air sac, and lungs. This study aimed to isolate Aspergillus sp. from the lungs of sparrows. The samples used in this research were the lungs of 35 sparrows taken randomly. Isolation was carried out by Thompson’s method (1969), samples were embedded in Saboraud’s Dextrose Agar (SDA) media and then incubated for 2-7 days. The growth of Aspergillus sp. colonies was observed at 24-hour intervals by looking at the color, shape, edges, and bottom surface of the colonies. Meanwhile, to ensure and confirm the species of Aspergillus sp., the fungus was planted on slide culture media. After that, microscopic observations were conducted by looking at the growth of conidiophores, hyphae, phialids, and fungal conidia. The data obtained were then analyzed descriptively. The result showed that Aspergillus sp. can be isolated from 27 out 35 samples. Based on the result of this study it can be concluded that 77.14% of samples were infected by Aspergillus sp. consisting of Aspergillus niger and Aspergillus flavus.
Co-Authors . Darniati Azhari Azhari Erina Erina Fitri, Khairani Hennivanda, Hennivanda M Daud AK Masda Admi Nugraha, Ronaldi Fajar Rosmaidar Rosmaidar, Rosmaidar Syafruddin Syafruddin Winaruddin Winaruddin