Anak Agung Gde Oka Dharmayudha
Laboratorium Bedah Dan Radiologi Veteriner, Universitas Udayana

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Journal : Jurnal Veteriner

The Differences of Ultrasonography Imaging Between Green Turtles (Chelonia mydas) and Olive Ridley Turtles (Lepidochelys olivacea) in Bali (PERBEDAAN CITRA ULTRASONOGRAFI ANTARA PENYU HIJAU (CHELONIA MYDAS) DAN PENYU LEKANG (LEPIDOCHELYS OLIVACEA) DI BALI I Wayan Nico Fajar Gunawan; Archie Leander Maslim; Ida Bagus Windia Adnyana; Anak Agung Gde Oka Dharmayudha; Luh Made Sudimartini
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Ultrasonography is a rapid and non-invasive method for assessing internal organs. The purpose of this study is to determine the differences in ultrasonographic imaging of internal organs between green turtle (Chelonia mydas) and olive ridley turtles (Lepidochelys olivacea). This study used two female green turtles (C. mydas) and two olive ridley turtle (one male and one female) (L. olivacea) with body condition index ranging from Average-Very Good. Micro-convex transducer ultrasonography with frequencies 4.5-8 MHz was used in this study. Acoustic windows were used in this study from cervical dorsal, cervical ventral, cervicobrachial, sinister-dexter, axillary sinister-dexter, prephemoral sinister-dexter and postphemoral sinister-dexter. The necropsies were performed to provide reference data. The results of ultrasonography imaging showed that the jugular vein of the olive ridley turtles (L. olivacea) was wider than the jugular vein of the green turtles (C. mydas). The ultrasonography imaging also showed that the stomach of green turtles (C. mydas) was smooth folds but the stomach of olive ridley turtles (L.olivacea) was sharp folds according to necropsy. There were no differences in the heart, stomach, liver, small intestine, large intestine, and kidneys. Vitellogenic follicles ultrasonography imaging was found from the olive ridley turtles (L. olivacea) which had shown in the period of premating and mating.
Case of Entamoebiasis in Pigs Raised with a Free Range Systems in Bali, Indonesia (KASUS ENTAMOEBIASIS PADA BABI YANG DIPELIHARA DENGAN CARA DIUMBAR DI BALI, INDONESIA) Kadek Karang Agustina; Anak Agung Gde Oka Dharmayudha; Ida Bagus Made Oka; I Made Dwinata; I Made Kardena; Nyoman Sadra Dharmawan; I Made Damriyasa
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aims were to measure the prevalence of Entamoeba in pigs in Bali and to identify thezoonotic potential species of Entamoeba. A total of 183 pig stool samples from Bali have been examined.The method being used in this study were combination between coproscopy and molecular techniques.Concentration sedimentation with Sodium Acetic Formaldehide (SAF) as a solution was used in thecoproscopy method, while the Polimerase Chain Reaction method was used to amplify DNA of Entamoeba.Extracted sample’s DNA examined by using primers that specifically for Entamoeba: Entam 1 (F) (5’-GTTGAT CCT GCC AGT ATT ATA TG-3’) and Entam 2 (R) (5’-CAC TAT TGG AGC TGG AAT TAC-3), and toidentify the zoonotic potential species of Entamoeba, samples that produce 550 bp in first amplificationcontinued by primers Epolecki1 (F) (5’-TCG ATA TTT ATA TTG ATT CAA ATG-3’) and Epolecki2 (R) (5’-CCT TTC TCC TTT TTT TAT ATT AG-3’). The results showed that 76.6% of samples were positive incoproscopical examination, but 84.7 % produced 550 bp bands on PCR amplification by using generalprimers. All positive samples on the first PCR continued to second PCR used specific primers for E.poleckii as a potential zoonotic disease and all of the samples showed negative results. This datademonstrated that the prevalence of Entamoeba in a traditional pig scavenging systems in Bali was 84.7%but no specific infection infection caused by E. polecki was found.
Radiographic Evaluation of Rabbit Femur Implanted Bali Cattle Bone Graft I Wayan Wirata; Steven Dwi Purbantoro; Luh Made Sudimartini; IWN Fajar Gunawan; AA Oka Dharmayudha; IGAG Putra Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bone xenograft from cattle bone is commonly used to treat a comminuted fracture case. This study aims to know the process of fractured-femur bone healing in rabbit post-implantation powder bone graft from cortical femur bone of Bali cattle evaluated by radiographs. Ten male local rabbits were used in this study, which were divided into 2 groups randomly. Group I (KI) as control, the diaphysis of femur bone was drilled with a diameter of 5 mm without implanting the bone graft, while Group II (KII), the diaphysis of femur bone was drilled 2 holes with a diameter of 5 mm each and with distance 20 mm, substituted with mineralized powder bone graft for the proximal hole (KIIa) and demineralized powder bone graft for the distal hole (KIIb). Fracture healing evaluation was done at week 0 (24 hours), 2, 4, and 6 postoperative by monitoring the growth of callus, fracture line, and union process with radiograph based classification according to Hammer et al., tabulated statistically, and presented descriptively. The results showed that KI and KII were in the sequel of fracture healing but had not reached remodeling phase perfectly. In conclusion, mineralized and demineralized powder bone graft used in this study was as osteoconductive and the use of bone graft shows no different significance and time shows different significance to fracture healing.