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All Journal VALENSI Jurnal Kimia (Journal of Chemistry) Journal of Mathematical and Fundamental Sciences Jurnal Ilmiah Arena Tekstil Padjadjaran Journal of Dentistry Chimica et Natura Acta Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Makara Journal of Science Indonesian Scholar Journal of Nursing and Midwifery Science
Euis Julaeha
Universitas Jenderal Achmad Yani
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health

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Formulation of Mouthwash for Gingivitis from Combination Infusion of Salam leaves (Eugenia Polyantha Wight) and Betel leaf (Piper betle. L) Putri, Megananda Hiranya; Julaeha, Euis; Herijulianti, Eliza; Nurjanah, Neneng
Padjadjaran Journal of Dentistry Vol 34, No 3 (2022): November 2022
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no3.41509

Abstract

ABSTRACTIntroduction: Gingivitis is inflammation of the gingiva associated with bacterial activity in dental plaque. The combination of bay leaves and betel leaves infusion has been used as a toothbrush disinfectant. The purpose of this study was to obtain a stable and effective mouthwash formulation from the combination of both infusions to prevent and relieve gingivitis. Methods: Five mouthwash formulas were made by mixing the active ingredients which is a combination of bay leaves and betel leaves infusion, with additives. The combination namely F-I (100% : 0%); F-II (75% : 25%); F-III (50% : 50%); F-IV (25% : 75%) and F-V (0%: 100%). Antibacterial activity tests against Streptococcus mutans, Streptococcus sanguis, and Porphyromonas gingivalis and physical stability tests were carried out. Stability test was carried out by storing the formula at room temperature (280C), hot temperature (400C) and cold temperature (40C) for 3 storage cycles (6 weeks) and observing changes in the physical indicators of the solution, namely pH, specific gravity, viscosity and organoleptic conditions, namely homogeneity, clarity, color, aroma and taste at the end of every 2nd week. Results: F-I to F-V had no inhibitory activity against Streptococcus mutans and Streptococcus sanguis, but had inhibitory activity against Porphyromonas gingivalis in the range (7.19±0.48) to (8.29 ±0.82) mm (ANOVA with a significance value of 0.237 > 0.05).  The 5 formulas were more stable at cold storage, with organoleptic observations at the end of the 3rd cycle showing a slight precipitate (+), clear solution, light brown color, mint aroma and sweet taste. F-II formulas showed better physical indicators values because they were closer to the standard values. Conclusion:  The F-II formula is recommended as a mouthwash for gingivitis because significant for antibacterial effect and showed better physical indicators values as the standard values for herbal medicines.Keywords: antibacterial activity; bay leaves - betel leaves infusion; gingivitis; mouthwash formulation.
Chemical composition of Citrus spp. and oral antimicrobial effect of Citrus spp. peels essential oils against Streptococcus mutans Aripin, Dudi; Julaeha, Euis; Dardjan, Murnisari; Cahyanto, Arief
Padjadjaran Journal of Dentistry Vol 27, No 1 (2015): March 2015
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol27no1.26751

Abstract

Introduction: The essential oils of orange peels had a considerable potential to be used as an antimicrobial agent. The aim of this present study is to analized chemical composition of Citrus spp. And the oral antimicrobial effect of Citrus spp. peels essential oils against Streptococcus mutans. Methods: Five orange peels species were used in this study consist of Lime (Citrus aurantifolia), Tangerine (Citrus nobilis), Sweet Orange (Citrus sinensis), Lemon (Citrus limon), and Kaffir Lime (Citrus hystrix). The isolated essential oils were analyzed using gas chromatography-mass spectroscopy (GC-MS). The Streptococcus mutans ATCC 25175 was employed against the antimicrobial effect of samples. Results: The extraction yields of hydrodistilled-essential oils from Tangerine, Kaffir Lime, Sweet Orange, Lemon, and Lime provided the extraction yields of 4.20, 2.26, 1.97, 1.74 and 0.83% yields, respectively. Major component essential oils of Citrus spp. was D-Limonene. The highest antimicrobial activity against S. mutans was Lime peel essential oil, followed by Lemon, Kaffir Lime, and Sweet Orange or Tangerine. All samples showed antimicrobial activity against S. mutans with the variation of antimicrobial action depending on the constituent of D-Limonene, β-Pinene, and α-terpineol. Conclusion: Major component chemical composition of essential oils of Citrus spp. was D-Limonene and antimicrobial activity by Lime peel essential oil due to its proportional amount of D-limonene and β-pinene and the highest antimicrobial activity.
Formulation of mouth rinse from the essential oils of lime (Citrus aurantifolia) and its inhibitory efficacy on the growth of Streptococcus mutans – in vitro Mulyanti, Sri; Laela, Dewi Sodja; Julaeha, Euis; Suwargiani, Anne Agustina; Aripin, Dudi
Padjadjaran Journal of Dentistry Vol 32, No 1 (2020): March 2020
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol32no1.25486

Abstract

Introduction: Essentials oil of lime (Citrus aurantifolia) peel has high activity in inhibiting the growth of Streptococcus mutans bacteria. The objective of this research was to determine the formulation of mouth rinse from the essential oils of lime (C. aurantifolia) with the highest inhibitory and on the growth of S. mutans. Methods: This research was an experimental in-vitro study. The sample used was the lime (C. aurantifolia) peel waste peel using hydrodistillation technique; testing of the essential oil quality was performed using 100°C distilled water for 3 hours; organoleptic test and physical mouth rinse formula test was also conducted. Mouth rinse formulations were made from the essential oils in the concentrations of 1, 2, 4, 8, 10, and 20%. Mouth rinse activity was tested with optical density. Minimum inhibitory concentration was determined with microdilution. Results: Essential oil mouth rinse formulation showed antibacterial activity at the concentrations of 2, 4, 8, 10, and 20% (formulations I-IV); the recommended mouth rinse concentration was 4% (formulation III) due to its highest antibacterial. Formulation IV showed the highest minimum inhibitory concentration value of 5.2 mg/mL (0.52%) and the safe minimum bactericidal concentration of 0.4 mg/mL (1.04). Conclusion: Mouth rinse formulation from essential oils of lime (C. aurantifolia) peel begins to show inhibition of the growth of S. mutans bacteria at the concentration of 2% essential oils (formulation II), with inhibition against the growth of S. mutans is 7.08. The formula shows a minimum inhibitory concentration (MIC) value and a minimum bactericidal concentration (MBC) of 5.2 mg/mL (0.52%) and 10.4 mg/mL (1.04), respectively.
The antibacterial effect of β-pinene derived from Citrus aurantifolia peel against oral Streptococcus mutans ATCC 25175 Julaeha, Euis; Herlina, Tati; Nurzaman, Mohamad; Mayanti, Tri; Kurnia, Dikdik; Sari, Elizabeth Fitriana
Padjadjaran Journal of Dentistry Vol 33, No 1 (2021): March 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no1.29200

Abstract

Introduction: Streptococcus mutans has been known to play a major role in dental caries development. This tooth decay is the most common oral disease affecting people in the world. Hence, discovering the new herbal antibacterial agent seems to become more promising yet challenging. One of natural antibacterial source is the peel of Citrus aurantifolia, as it may contain antibacterial active compounds against pathogenic oral microbes. This study was aimed to isolate antibacterial compound from essential oil of Citrus aurantifolia and to analyse its antibacterial activity against oral Streptococcus mutans ATCC 25175. Methods: Type of research was laboratory experimental. The essential oil was isolated from peel of Citrus aurantifolia by hydro-distillation technique and further isolation of antibacterial compounds was conducted by combination column chromatography using organic solvent, and the structure was determined by UV-Vis, infrared (IR), 1D NMR of 1H-, 13C-NMR and DEPT 135° in CDCl3 spectrometers methods. The antibacterial activity was tested against Streptococcus mutans ATCC 25175 using the Kirby-Bauer method. Results: 0.75% yield was obtained from the essential oil of peel Citrus aurantifolia and after purification, an antibacterial compound was identified as β-pinene with the molecular formula C10H16. Furthermore, the β-pinene from peel of Citrus aurantifolia exerted inhibition growth against Streptococcus mutans at concentration of 2000, 1000, and 500 ppm by showing respectively, inhibition values of 13.0, 11.9, and 11.6 mm on paper disk. Conclusion: In the present study, the essential oil of Citrus aurantifolia peel is proven to contain prospective antibacterial compound (β-pinene) that potentially can be used as natural antibacterial alternative choice to manage oral disease associated with Streptococcus mutans infection.
Co-Authors Achmad Zainuddin Ade Kania Ningsih, Ade Kania Ajeng Diantini, Ajeng Anne Agustina Suwargiani Arif Rahman Hakim Cahyanto, Arief Dikdik Kurnia Dudi Aripin Elizabeth Fitriana Sari Faizal Hermanto Hadi Kuncoro Herijulianti, Eliza IMAN PERMANA MAKSUM Kindi Farabi Laela, Dewi Sodja Laode Rijai Lilis Siti Aisyah, Lilis Siti Megananda Hiranya Putri Mohamad Nurzaman Mulyawan, Agus Surya Murnisari Dardjan, Murnisari Neneng Nurjanah Nurcahyanti, Ois Permadi, Nandang Shabarni Gaffar -, Shabarni Gaffar Sri Mulyanti Sugiyana, Doni Tati Herlina Tri Mayanti Tri Reksa Saputra, Tri Reksa Unang Supratman Wahyudi, Tatang Wibisono, Aditya Seiza Yenny Febriani Yun, Yenny Febriani Yoshihito Shiono Yudi Rosandi Yulisar, Riza