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Penyediaan Obat Penyakit Kulit Dan Luka Dari Cairan Fermentasi Lidah Buaya Dan Daun Sirih Riyanti, Fahma; Desnelli, Desnelli; Fatma, Fatma; Yuliasari, Nova; Julinar, Julinar; Musifa, Eva
Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Vol. 5 No. 3 (2025): Mei 2025 - Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat
Publisher : Indonesian Scientific Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59395/altifani.v5i3.693

Abstract

Cairan Fermentasi anaerob pada lidah buaya dan daun sirih mengandung enzim-enzim, beberapa senyawa kimia, dan sumber antimikroba alami. Kandungan ini memiliki kemampuan terhadap antibakteri dan anti jamur yang bermanfaat untuk mengobati penyakit kulit, luka dan untuk kesehatan kulit. Proses penyembuhan dapat dipercepat karena senyawa antibakteri menstimulator respon imun tubuh sehingga dapat menyembuhkan luka dan penyakit kulit. Kegiatan pengabdian yang berupa penyuluhan, praktek pembuatan cairan fermentasi lidah buaya - daun sirih dan pemberian produk yang sudah jadi merupakan metode yang dilaksanakan di Desa Permata Baru Kecamatan Indralaya Utara Kabupaten Ogan Ilir. Agar peserta penyuluhan memahami tujuan kegiatan maka dilakukan praktek pembuatan cairan fermentasi dan peserta penyuluhan yang dibagi menjadi 3 kelompok. Pelaksanaan kegiatan dilaksanakan sebanyak tiga kali. Pelaksanaan kedua adalah memantau cairan fermentasi yang dibuat sebelumnya. Hasil fermentasi dibuka setelah 3 bulan, ketiga cairan fermentasi tersebut berhasil dibuat ditandai aroma asam segar dan warna coklat.
Response Surface Methodology to Optimize Laccase Produced from Pleurotus ostreatus Using Corn Cob and Rice Bran as Substrate Hermansyah, Hermansyah; Gozali, Maftuh; Julinar, Julinar; Fanani, Zainal; Loekitowati Hariani, Poedji
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.14472

Abstract

ABSTRACT. Laccase is an industrially significant enzyme capable of oxidizing a broad range of phenolic compounds, making it valuable for applications such as bioremediation, textile dye decolorization, and pulp bleaching. Therefore, this study aimed to optimize laccase production from Pleurotus ostreatus using agricultural waste substrates corn cob (BJ), rice bran (DP), and their 1:1 mixture (BJ:DP) through Response Surface Methodology (RSM). Extracellular laccase was obtained by centrifuging the cultured medium, followed by ammonium sulfate precipitation and dialysis. The enzyme activity was quantified, and the fraction with the highest specific activity was analyzed using SDS-PAGE. Furthermore, Central Composite Design (CCD) was used to assess the effects of three independent variables, namely ABTS concentration (0.01–0.1 mM), incubation temperature (20–30 °C), and reaction time (20–30 minutes). The results showed that for BJ substrate, the highest enzyme activity (13.7 ± 0.05 U/mL) was observed in the 0–20% ammonium sulfate fraction, with specific activities of 76.25 ± 0.09 U/mg and 90.28 ± 0.03 U/mg in the 0–20% and 20–40% fractions, respectively. Conversely, DP substrate achieved a maximum specific activity of 209.67 ± 0.028 U/mg in the 20–40% fraction. The crude extract from BJ:DP mixture showed a high protein content (0.636 ± 0.006 mg/mL) but the specific activity was substantially lower (19.33 ± 0.003 U/mg). Based on RSM analysis, the optimal conditions were ABTS concentration of 0.05 mM, incubation temperature of 22.21 °C, and reaction time of 28.64 minutes, resulting in a predicted laccase activity of 13.99 U/mL. Keywords: Corn cob, Laccase, Pleurotus ostreatus, Response Surface Methodology, Rice bran
Ecoenzyme Characterization of Fruit Peel Waste Mixture and Test of Antibacterial Activity against Bacteria Causing Dental Caries Julinar, Julinar; Uswati, Dinii; Riyanti, Fahma; Musifa, Eva
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 3 (2025): October 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Abstract

Ecoenzyme is a product of facultative aerobic fermentation for 3 months from organic fruit and vegetable waste. In this study, ecoenzyme was made from a mixture of fruit peel waste (papaya, orange and pineapple) with the addition of 6 liters of water. Ecoenzyme products were characterized by organoleptic properties, chemical composition, enzyme activity and antibacterial activity tests against Lactobacillus acidophilus and Streptococcus mutans bacteria that cause dental caries. The results of the study show that the ecoenzyme product had a dominant orange peel sour flavor, was cloudy brown in color with a volume of 8.4 liters from 6 liters of added water. The characterization results depict that the chemical composition of ecoenzyme contained acetic acid and lactic acid with a total acid content of 2.26%, 40.45 mg/mL protein, and secondary metabolites in the form of alkaloids, flavonoids, and saponins. The activity of several enzymes from ecoenzyme was protease 0.0246 U/mL, amylase 0.0032 U/mL and lipase 5 U/mL. Antibacterial activity against bacteria that cause dental caries is included in the category of very strong against S. mutans at a concentration of 40 (% v/v) and moderate against L. acidophilus with inhibition zone diameters of 23.33 mm and 8.23 mm respectively. The Mininum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MKC) values of ecoenzyme against S. mutans and L. acidophilus bacteria were 2500 ppm and 5000 ppm respectively.Keywords: ecoenzyme, fruit-peel waste, L.acidophilus, S.mutans, antibacterial
Hubungan Antara Bystander Effect dengan Perilaku Prososial pada Mahasiswa Pekanbaru Julinar, Julinar; Syarif, Khairunnisa’; Aiyuda, Nurul
Indonesian Research Journal on Education Vol. 4 No. 3 (2024): irje 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/irje.v4i3.787

Abstract

Perilaku prososial adalah perilaku yang terdapat dalam diri individu yang beguna untuk membantu orang lain, tanpa mengharapkan imbalan apapun dari penerima bantuan. Salah satu faktor yang mempengaruhi perilaku prososial yaitu bystander effect. Bystander effect merupakan suatu kondisi dimana individu memilih untuk tidak melakukan apapun saat terjadi suatu keadaan darurat, dikarenakan terdapat orang lain ditempat yang sama sehingga membuat individu merasa takut untuk terlibat dalam situasi tersebut dan cenderung memilih untuk tidak melakukan apapun karena menganggap bahwa orang lain lebih dapat diandalkan. Penelitian ini bertujuan untuk melihat hubungan antara bystander effect dengan perilaku prososial pada mahasiswa Pekanbaru. Penelitian ini menggunakan metode kuantitatif dengan instrument skala bystander effect dan skala perilaku prososial. Subjek dalam penelitian ini 350 mahasiswa di Pekanbaru yaitu 76 laki-laki dan 274 perempuan dengan rentang usia dari 18-35 tahun. Teknik pengambilan sampel menggunakan probability sampling dengan metode cluster random sampling. Berdasarkan hasil analisis korelasi pada penelitian ini terdapat hubungan negatif antara bystander effect dengan perilaku prososial dengan nilai signifikan 0,000, yaitu semakin tinggi bystander effect maka semakin rendah perilaku prososial, sebaliknya semakin rendah bystander effect maka akan semakin tinggi perilaku prososial. Hasil penelitian ini dapat digunakan untuk memberikan kesadaran terkait pentingnya meningkatkan perilaku prososial guna menghindari terjadinya bystander effect.
Biosourced Reduced Graphene Oxide and Its Application for Methylene Blue Adsorption Rachmat, Addy; Ahadito, Bijak Riyandi; Suryani, Sri; Meiliza, Cici; Hidayati, Nurlisa; Julinar, Julinar; Said, Muhammad
The Journal of Pure and Applied Chemistry Research Vol. 14 No. 3 (2025): Edition September-December 2025
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2025.014.03.7926

Abstract

Graphene oxide and reduced graphene oxide have been widely studied as a biomass-sourced alternative to graphene for their various capabilities, e.g. as adsorbent. This publication reports the preparation of graphite (g), graphene oxide (GO), and reduced graphene oxide (rGO) from coconut shell (1) and sugarcane bagasse (2) using matoa leaves extract as reducing agent, characterized by XRD, FT-IR, and Raman spectroscopy. The X-ray diffractogram showed 2θ peaks between 22.8°–23.8° for g1, GO1, and rGO1, and between 23.6°–25.8° for g2, GO2, and rGO2. FT-IR spectra of both sets of GO and rGO showed common peaks of O–H (~3400 cm–1), C–H (~2900 cm–1), and C–OH (~1600 cm–1), while also some noticeable differences between the sets. Raman spectra of both rGO showed D peak (~1400 cm–1), G peak (~1700 cm–1), and weak 2D peaks (2700–3300 cm–1) with ID/IG of 0.93 and 0.91 for rGO1 and rGO2, respectively. The obtained g, GO, and rGO were used as adsorbent for methylene blue dye. The adsorption study involved variation in dye concentration, contact time, and amount of adsorbent. It was found that rGO performs best as adsorbent compared to GO and g, with maximum adsorption capacity of 22.308 μg/mg (rGO1) and 47.533 μg/mg (rGO2). Adsorbents prepared from sugarcane bagasse were found to perform better due to its easiness of carbonization compared to coconut shell.