Ni Kadek Mulyantari
Bagian SMF Patologi Klinik, Fakultas Kedokteran Universitas Udayana

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PENDERITA DENGAN HEMOKROMATOSIS PRIMER Kadek Mulyantari; A.A.Wiradewi Lestari; A.A.N. Subawa; Tjokorda Gede Oka; Sudewa Djelantik
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 2 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i2.1014

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Primary Hemochromatosis is a hereditary disease that occurs predominantly in man. Among men, clinical signs and symptomsfrequently appears on 40 years until more than 60 years of age. Meanwhile, the signs and symptoms among women appear on 50 yearsof age or after menopause. It is a very rare case in children or young adult. Secondary hemochromatosis can be differentiated fromprimary hemochromatosis based on existence of other underlying disease and secondary hemochromatosis often occurs in patient withmultiple blood transfusions. The diagnosis of primary hemochromatosis is confirmed by chromosomal test and liver biopsy to confirmthe liver damage caused by excessive iron accumulation. The main treatment of primary hemochromatosis is phlebotomy. The purposeof this method is to remove overload iron in body. In this case, the patient was man, unmarried, 51 years old, Australian. Four yearsago, he had complained about arthropathies, chronic asthenia, depression, decreased of concentration and sexual desire. Laboratoryevaluation revealed Ferritin level 2126 ug/L and transferrin saturation always more than 99%. Liver function tests also increasedsignificantly. Some of his family’s members have the same disease as he has. He was diagnosed as primary hemochromatosis and hadperformed phlebotomy routinely. After phlebotomy has done, he recovered based on clinical and laboratorial findings.
INTERLEUKIN-10 PLASMA DAN LIMFOSIT-T CD4+ PENDERITA TERINFEKSI HIV Kadek Mulyantari; Endang Retnowati; Nasronudin Nasronudin
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 1 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i1.351

Abstract

HIV & AIDS have become a global problem throughout the world including Indonesia. The incidence is rapidly increasing. Various treatment and efforts have been carried out but until now have not yet been succeeded, the mortality remains high. Examination of CD4+ lymphocyte-T which is carried out to determine the immune status and monitoring the treatment has some limitation. Besides the degradation of CD4+ lymphocyte-T, the increase of the disease progression is also followed by an increase of interleukin-10 as well. The determination of interleukin-10 is expected to be use as an alternative examination if CD4+ lymphocyte-T can not be performed. To analyze the correlation between the levels of plasma interleukin-10 and amount of CD4+ lymphocyte-T in the asymptomatic HIV infected patients. A cross sectional, observational analytical study of 41 patients with HIV infection stage I, has been conducted from March–April 2009, at the Intermediate Infectious Disease Care Unit Dr. Soetomo General Hospital, Surabaya. The diagnosis of HIV patients was based on positive result of HIV test using three (3) different methods. Eight mL venous blood were taken from each patient, 6 mL is put into a heparin tube for the examination of IL-10 plasma with ELISA method and 2 mL is put into a K3EDTA tube for examining the CD4+ lymphocyte-T using flowcytometry. The results showed of plasma IL-10 level and the amount of CD4+ lymphocyte-T, which were analyzed by Pearson correlation test to determine the correlation between the two (2) variabels. The IL-10 levels in HIV patients were 3.80–44.50 pg/mL (mean 18.09 pg/mL, SD 8.84 pg/mL). The amount of absolute CD4+ lymphocyte-T was 5–846 cells/µL (mean 322.07 cells/µL and SD 221.89 cells/µL), while the amount of percentage CD4+ lymphocyte-T was 0.41–29.48% (mean 13.99%, SD 7.62%). Statistical analyzes show a significant negative correlation either between plasma IL-10 level with absolute CD4+lymphocyte-T and plasma IL-10 level with percentage of CD4+ lymphocyte-T, e ach level of r was –0.652 and –0,683. A significant negative correlation was also formed between plasma IL-10 level and the amount of CD4+ lymphocyte-T in the HIV infected patients. In the HIV infected patients, the increase of plasma IL-10 level was followed by a decrease of CD4+ lymphocyte-T.
AUDIT SYSTEM FOR USAGE OF BLOOD PRODUCT IN SANGLAH GENERAL HOSPITAL DENPASAR BALI Kadek Mulyantari; Teguh Triyono; Usi Sukorini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1382

Abstract

Blood transfusion is one of very important therapy components. One indicator of the blood services quality in Sanglah  General Hospital Denpasar Bali Indonesia is achieving the target of unused blood order ≤ 10% per month. However, these targets have not been achieved. The number of unused blood orders reached 6,719 bags per year (600 bags per month or 21.5%). Unused blood orders is mostly from Department of Internal Medicine (40.9%), followed by Department of Surgical (35.6%),  Department of Obstetrics and Gynecology (15.5%) , and Department of Pediatrics (8%). The high number of unused blood orders can causes several problems such as the disorder of blood supply, inefficiency of pre-transfusion testing and decreased quality of blood product. Considering the consequences of high unused blood order, it is necessary to conduct a research on audit system for usage of blood product in Sanglah Hospital.The aim of study are to analyze the influence of audit system to reduce unused blood order and to decrease the number of C/T ratio. The study design was a pre and post test intervention study. The population in this study were all forming of blood requests in Sanglah Hospital. The sample is blood request form Department of Internal Medicine of the last two months. Intervention in this study was the development and dissemination of guidelines for usage of blood products, followed by concurrent audit and prospective audit of the sample.The study show there are decline of unused blood orders and C/T ratio before and after implementation of the audit system for usage of blood products. Reduction of unused blood order in Sanglah Hospital is 5.4%, and in the Department of Internal Medicine is 5.2%. The causes of unused blood orders were patient died, overestimation of blood order, transfusion delays because waiting for the schedule of hemodialysis, the patient has a fever, blood product has expired, and the occurrence of administration errors. Reduction of C/T ratio in Sanglah Hospital is 0,094 and in the Department of Internal Medicine is 0,072. Audit system for usage of blood products can reduce unused blood orders and decrease C/T ratio.
Gambaran hasil pemeriksaan faal hemostasis pada penderita Diabetes Melitus tipe-2 (DM-2) di RSUP Sanglah, Bali, Indonesia Nyoman Pramudita; Ni Kadek Mulyantari
Intisari Sains Medis Vol. 10 No. 2 (2019): (Available online: 1 August 2019)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.737 KB) | DOI: 10.15562/ism.v10i2.392

Abstract

Background: Diabetes Mellitus Type II (DM-2) is a type of hyperglycemic state which is related to metabolic disorders caused by mutations in several genes. Faal hemostasis is a function of the body that aims to maintain the blood that has been thinned so that the blood flowing in the veins and could be interfere with blood glucose level. This study aims to describe the faal hemostasis examination in patients with DM-2 in Sanglah Hospital.Methods: A cross-sectional study was conducted among patients with diabetes mellitus type II (DM-2) who were hospitalized in Sanglah hospital at the period started from January 1st to December 31st, 2015. The sample selection were using consecutive sampling with a total of twenty eight samples. Data were analyzed using SPSS version 17 for WindowsResults: The study found that males were predominant in this study (53.6%). In addition, 51-60 years age group were also predominant (39.3%), following by history of hypertension (82.1%), 10-14 seconds of Prothrombin Time (PT) (67.9%), 30-40 seconds of Activated Partial Thromboplastin Time (APTT) (53.5%), and bleeding time (BT) > 1.3 minutes (67.9%).Conclusion: Several faal hemostasis assessments were predominant in patients with DM-2 such as 10-14 seconds of PT, 20-40 seconds of APTT, and bleeding time more than 1.3 minutes.
Korelasi jenis dan lama waktu pemberian obat antituberkulosis paru terhadap kadar glukosa darah di RSUP Sanglah periode 2015-2016 Gde Dicky Arimbawa; Ni Kadek Mulyantari
Intisari Sains Medis Vol. 10 No. 3 (2019): (Available online: 1 December 2019)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.631 KB) | DOI: 10.15562/ism.v10i3.449

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Background: Tuberculosis (TB) is a chronic infectious disease caused by an infection of the Mycobacterium tuberculosis complex. Antituberculosis (OAT) drugs used in TB treatment include Isoniazid (H), Rifampicin (R), Pyrazinamide (Z), Ethambutol (E), and Streptomycin (S). The effects of OAT use are many that are hepatotoxic. This hepatotoxic disrupts liver function as a very important glucose buffer system. The purpose of this study was to determine the correlation of the type and duration of administration of OAT to blood glucose levels at Sanglah Hospital.Method: This study is an analytical study with a cross sectional study design. The population in this study were tuberculosis patients who were treated at Sanglah General Hospital, Denpasar in March 2015 - October 2016. The selection of samples used consecutive sampling with a total sample of 38.Results: The correlation test results between the duration of OAT administration and blood glucose levels in tuberculosis patients at Sanglah General Hospital using the spearman test obtained a correlation coefficient (r) value of 0.536 so that according to statistics it can be concluded that there is a positive correlation between the length of time OAT is to blood glucose concentrations. The correlation test between the type of OAT on blood glucose levels in tuberculosis patients in Sanglah General Hospital used the chi-square test obtained P value is 0.133 so according to statistics it can be concluded that there was no significant relationship between the type of OAT to blood glucose concentrations in tuberculosis patients at Sanglah Hospital.Conclusion: There is a significant positive correlation between the duration of OAT treatment of blood sugar levels, but there is no relationship between the type of OAT to blood sugar levels in patients with pulmonary tuberculosis.
Analisis korelasi kadar serum prokalsitonin dengan jumlah leukosit pada penderita dengan kecurigaan sepsis di RSUP Sanglah, Bali, Indonesia Bagus Dwiki Arya Dharma; Ni Kadek Mulyantari; I Putu Yuda Prabawa
Intisari Sains Medis Vol. 11 No. 1 (2020): (Available online: 1 April 2020)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.223 KB) | DOI: 10.15562/ism.v11i1.525

Abstract

Background: Sepsis is a systemic inflammatory response to infection, where the pathogen or toxin is released into the blood circulation, causing activation of the inflammatory process. Laboratory tests are used to detect inflammatory processes, one of which is procalcitonin. This study aims to determine the correlation between procalcitonin (PCT) serum levels by the leukocytes counts on suspected sepsis patients at Sanglah Hospital in Denpasar.Methods: An analytic observational study with the cross-sectional design was conducted among 21 samples by consecutive sampling technique. The population in this study were sepsis patients who were treated at Sanglah Hospital Denpasar during February-June 2016 period. Data were analyzed using SPSS version 17 for Windows. Results:  Most of respondents were >35 years old (85.7%), Body Mass Index (BMI) > 20 kg/m2 (76.2%), leucocyte counts < 10.000/μL (42.9%), and PCT levels ≤ 0.5 ng/mL (61.9%). There is no significant correlation between white blood cells (WBC) and PCT by Spearman Correlation test (p=0.061; r=0,641).Conclusion: The recent findings suggest that there was no significant correlation between PCT serum levels with the leucocytes counts among patients with sepsis. Latar Belakang: Sepsis merupakan suatu respon inflamasi sistemik terhadap infeksi, dimana patogen atau toksin dilepaskan ke dalam sirkulasi darah sehingga terjadi aktivasi proses inflamasi. Tes laboratorium yang digunakan untuk mengetahui adanya proses inflamasi salah satunya adalah prokalsitonin (PCT). Penelitian ini bertujuan untuk mengetahui korelasi antara kadar serum prokalsitonin dengan jumlah leukosit pada penderita dengan kecurigaan sepsis di RSUP Sanglah Denpasar.Metode: Penelitian observasional analitik dengan desain tong lintang dilakukan terhadap 21 sampel dengan teknik konsekutif. Populasi dalam penelitian ini adalah pasien sepsis yang dirawat di Rumah Sakit Sanglah Denpasar selama periode Februari-Juni 2016. Data dianalisis menggunakan SPSS versi 17 untuk Windows.Hasil: Sebagian besar responden berusia> 35 tahun (85,7%), Indeks Massa Tubuh (IMT)>20 kg / m2 (76,2%), jumlah leukosit <10.000 / μL (42,9%), dan tingkat PCT ≤ 0,5 ng / mL (61,9%). Tidak terdapat korelasi yang bermakna antara sel darah putih (WBC) dan PCT dengan uji Korelasi Spearman (p = 0,061; r = 0,641).Kesimpulan: Hasil penelitian saat ini menunjukkan bahwa tidak terdapat korelasi yang bermakna antara kadar serum PCT dengan jumlah leukosit pada pasien dengan sepsis.
Aberrant expression of CD5 in a B-lineage Acute Lymphoblastic Leukemia (ALL): a case report Ni Ketut Puspa Sari; Ni Nyoman Mahartini; Ni Kadek Mulyantari; Anak Agung Ayu Lydia Prawita; Ni Komang Krisnawati; I Putu Yuda Prabawa; Sianny Herawati; Made Minarti Witarini Dewi; Ekarini Katharina Yunarti Nabu
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.872 KB) | DOI: 10.15562/ism.v12i2.1011

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Background: CD5 is expressed in several B-lymphocyte malignancies, including Chronic Lymphocytic Leukemia (CLL), Mantle Cell Lymphoma (MCL), but CD5 positive B-cell lineage Acute Lymphoblastic Leukemia (ALL) is extremely rare. Aberrant T-cell antigen expression is associated with poor prognosis and is a useful marker to identify patients at increased risk. This case report aims to elaborate on the aberrant expression of CD5 in B-lineage ALL.Case Presentation: A 16-years-old male presented with a history of gum bleeding and abdominal pain of 7 days with associated fever, weakness and bone pain of 2-month duration. The bone marrow aspiration showed lymphoblast 30 % with the positive vacuole. In addition, the immunophenotyping test indicates for leukemia B-lineage with expression CD19, CD10, HLA-DR, CD 34, and there is the aberrant expression of CD5.Conclusion: Aberrant expression CD5 in cases of ALL B lineage is very rare. This can be associated with a poor prognosis.
Sindroma Bartter pada laki-laki berusia 44 tahun: laporan kasus Anak Agung Ayu Lydia Prawita; Anak Agung Wiradewi Lestari; Sianny Herawati; Ni Kadek Mulyantari; Ni Komang Krisnawati; Ekarini Katharina Yunarti Nabu
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.828 KB) | DOI: 10.15562/ism.v12i2.1049

Abstract

Background: Bartter's syndrome is a hereditary condition characterized by polyuria, hypokalemia, metabolic alkalosis with normal or slightly low blood pressure due to loss of sodium and renal hyperplasia. Most Bartter syndrome occurs in children and is very rare in adulthood, with a prevalence of 1 in 1,000,000 populations. This case report aims to evaluate the laboratory aspects of Bartter's syndrome in a 44-year-old man at Sanglah Hospital, Bali, Indonesia.Case Presentation: A 44-year-old male patient complaining of vomiting and weakness. Other complaints such as diarrhea, fever, shortness of breath, stiffness and seizures are denied. The patient's previous medical history, treatment history, and family history were denied. Physical examination showed a general weakness with hypotension and polyuria. On laboratory examination, there was severe hypokalemia, hypocalcemia, hypomagnesemia, increased urinary potassium levels, increased urinary chloride levels, and metabolic alkalosis. To differentiate with Gitelman syndrome, an examination of the ratio of urine calcium and creatinine is performed. In this patient, the urine calcium and creatinine ratio increased, which indicated Bartter's syndrome. Patients receive electrolyte replacement therapy.Conclusion: Bartter syndrome is very rare in adulthood. Several tests are needed to make the diagnosis in accordance with the diagnostic approach of hypokalemia, namely electrolyte examination in urine serum, urine creatinine, and blood gas analysis.  Latar Belakang: Sindroma Bartter merupakan suatu kondisi herediter yang ditandai oleh poliuria, hipokalemia, metabolik alkalosis dengan tekanan darah normal atau sedikit rendah karena kehilangan natrium dan hiperplasia ginjal. Sebagian besar sindroma Bartter terjadi pada anak-anak dan sangat jarang terjadi pada usia dewasa dengan prevalensi 1 dalam 1.000.000 populasi. Laporan kasus ini bertujuan untuk mengevaluasi aspek laboratorium sindroma Bartter pada laki-laki berusia 44 tahun di RSUP Sanglah, Bali, Indonesia.Presentasi Kasus: Pasien laki-laki 44 tahun dengan keluhan muntah dan lemas. Keluhan lain seperti diare, demam, sesak nafas, kaku dan kejang tidak ada. Riwayat penyakit sebelumnya, riwayat pengobatan, dan riwayat penyakit pada keluarga disangkal pasien. Pada pemeriksaan fisik didapatkan keadaan umum lemah dengan hipotensi dan poliuria. Pada pemeriksaan laboratorium ditemukan hipokalemia berat, hipokalsemia, hipomagnesemia, peningkatan kadar kalium urine, peningkatan kadar klorida urine, dan alkalosis metabolik. Untuk membedakan dengan sindroma Gitelman dilakukan pemeriksaan rasio kalsium dan kreatinin urine. Pada pasien ini didapatkan hasil rasio kalsium dan kreatinin urine yang meningkat, yang mengindikasikan sindroma Bartter. Pasien mendapatkan terapi penggantian elektrolit .Kesimpulan: Sindroma Bartter sangat jarang terjadi pada usia dewasa. Beberapa pemeriksaan diperlukan untuk menegakkan diagnosis sesuai dengan alur pendekatan diagnostik hipokalemia yaitu pemeriksaan elektrolit pada serum urine, kreatinin urine, dan analisis gas darah.
Perbandingan kadar Thyroid Stimulating Hormone (TSH) dan kadar Free T4 (FT4) antara metode Fluorescence Immunoassay (FIA) dan metode Electrochemiluminescence Immunoassay (ECLIA) di RSUP Sanglah, Bali, Indonesia Ekarini Katharina Yunarti Nabu; Sianny Herawati; Ni Kadek Mulyantari; Anak Agung Wiradewi Lestari; I Putu Yuda Prabawa
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.92 KB) | DOI: 10.15562/ism.v12i2.1062

Abstract

Background: Diagnostic and therapeutic cases of hyperthyroidism can be identified by examining the levels of Thyroid Stimulating Hormone (TSH) and Free T4 (FT4). There are several examination methods, namely the Fluorescence Immunoassay (FIA) and the Electro-Chemiluminescence Immunoassay (ECLIA) method. This study aims to evaluate the difference in TSH and FT4 level in the FIA method and ECLIA method.Method: This study used observational analytic methods cross sectional design, with 45 samples serum patient with hyperthyroid in January-March 2019. Examine level TSH and FT4 levels with FIA methods (NANO ENTEK FRENDTM) and ECLIA method (Cobas e601 ROCHE). Data were analyzed using SPSS version 17 for Windows.Results: Most of the respondents were aged 25-34 years (28.8%) and female (86.7%). There was no significant difference in the mean TSH level on examination using the ECLIA method (0.60±0.79 mIU/L) and the FIA method (0.63±0.76 mIU/L) (p=0.639). In addition, there was no significant difference in FT4 both in the examination using the ECLIA method (1.67±1.05 ng/dl) and the FIA method (1.69±1.72 ng/dl) (p=0.745).Conclusions: There was no significant difference between TSH and FT4 in both ECLIA and FIA methods. Latar Belakang: Diagnosis maupun pemantauan terapi kasus hipertiroid dapat diketahui dengan pemeriksaan kadar hormon Thyroid Stimulating Hormone (TSH) dan Free T4 (FT4). Terdapat beberapa metode pemeriksaan yaitu metode Fluorescence Immunoassay (FIA) dan metode Electro-Chemiluminescence Immunoassay (ECLIA). Penelitian ini bertujuan untuk mengevaluasi perbedaan kadar TSH dan FT4 metode FIA dan metode ECLIA.Metode: Penelitian ini menggunakan metode observasional analitik dengan desain potong lintang pada 45 sampel serum penderita hipertiroid selama periode Januari-Maret 2019. Dilakukan pemeriksaan kadar TSH dan FT4 dengan metode FIA (NANO ENTEK FRENDTM) dan metode ECLIA (Cobas e601 Roche). Data dianalisis dengan SPSS versi 17 untuk Windows.Hasil: Sebagian besar responden berusia 25-34 tahun (28,8%) dan berjenis kelamin perempuan (86,7%). Tidak terdapat perbedaan bermakna rerata kadar TSH pada pemeriksaan menggunakan metode ECLIA (0,60±0,79 mIU/L) maupun metode FIA (0,63±0,76 mIU/L) (p=0,639). Disamping itu, tidak juga terdapat perbedaan bermakna rerata kadar FT4 baik pada pemeriksaan menggunakan metode ECLIA (1,67±1,05 ng/dl) maupun metode FIA (1,69±1,72 ng/dl) (p=0,745).Kesimpulan: Tidak ada perbedaan yang bermakna antara kadar TSH dan FT4 pada metode ECLIA dan FIA.
A child with Down Syndrome and Type 2 Diabetic Mellitus Betti Bettavia Hartama Pardosi; Sianny Herawati; Ni Kadek Mulyantari
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 28, No 3 (2022)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i3.1831

Abstract

Down Syndrome (DS) is one of the common chromosomal disorders that raise mental retardation. It is known that DS patients have an autoimmune disorder affecting the endocrine and non-endocrine organs. It is a rare occurrence of type 2 Diabetes Mellitus (type 2 DM) disease in children with DS. Type 2 DM occurs due to impaired insulin secretion and excessive hepatic glucose production, unlike type 1 DM, caused by the destruction of ß-cells in autoimmune Langerhans. A 10-year-old girl patient was referred from Tabanan Hospital to Sanglah Hospital, Denpasar. Patients were admitted to the hospital with decreased consciousness, treated for four days, and observed in ICU for 2 days. Vomiting twice, no seizures, urinating normally. No significant past medical history was found. Physical examinations showed a typical Mongolian face, short neck, expanded occipital area, small eyes, and a mouth with a prominent tongue. Laboratory data revealed fasting blood glucose of 473 mg/dL and an HbA1C level of 12.6%. Urinalysis showed ketone 3+. The C-peptide test showed a reasonably good ß pancreas cell function. Down syndrome is associated with autoimmune diseases, including type 1 diabetes. The exact number of down syndrome cases with type 2 DM remains unknown; however, it was known that the case is infrequent.