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All Journal Jurnal Medik Veteriner Journal of Parasite Science Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary
Rahaju Ernawati
Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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The Prevalence of Gastrointestinal Tract Helminthiasis Through Stool Examination in Cattle at Benowo Landfill Surabaya Ratih Prajnya Paramitha; Rahaju Ernawati; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 1 No. 1 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (609.829 KB) | DOI: 10.20473/jops.v1i1.16218

Abstract

Tujuan penelitian ini adalah untuk mengetahui prevalensi cacing, untuk mengetahui berbagai jenis telur cacing saluran cerna dan jenis umur terhadap infeksi cacing saluran cerna pada sapi di tanah Benowo, Surabaya. Sampel tinja diambil dari TPA sebanyak 41 sampel. Pemeriksaan tinja dilakukan dengan metode asli, sedimentasi sederhana dan pelapisan Fulleborn. Hasil penelitian menunjukkan bahwa 30 sampel positif menentang gastrointestinal, ini menunjukkan prevalensi cacing adalah 73%. Jenis telur cacing adalah kelas Nematode, yaitu Oesophagostomum sp., Trichostrongylus sp., Bunostomum sp. , Mecistocirrus digitatus, Trichuris sp dan Toxocara vitulorum.Menurut analisis statistik, dapat disimpulkan jenis dan jenis kelamin tidak dapat dihitung terhadap infeksi cacing.
Identifikasi dan Pola Infestasi Caplak pada Biawak Air (Varanus salvator, Byers, d. 2000) Kartika Aditiya Amelia; Rahaju Ernawati; Poedji Hastutiek; Boedi Setiawan; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.9 KB) | DOI: 10.20473/jops.v4i1.20274

Abstract

The aim of this study is to identify the type of ticks infestation pattern on water monitor lizard (Varanus salvator, Byers, D. 2000) with regard parameters water monitor lizard species, stadium and region of the water monitor lizard’s body. The total samples of 250 ticks from 30 water monitor lizard (18 male lizards and 12 female lizards) were collected every day starting from noon to evening during July to August 2019 at the monitor slaughtering place in Buduran District, Sidoarjo Regency. The tick samples were identified by the permanent mounting slide method without staining and put in bottles containing 10% KOH for 1-10 hours, then each stadium used a nikon eclipse E100 microscope with a 40x magnification binocular microscope. Data was analyzed using Multiple Correspondance Analytics. The result indicate that 30 V. salvator (100%) are positive infested by tick and the samples obtained consisted of 133 Aponomma sp. and 117 Amblyomma sp. in all regions of the body.
Isolation and Biological Characterization of Newcastle Disease Virus (NDV) Field Isolate Pigeon (Columba livia) from Live Bird Market, East Java in 2019 Venri Novryantoro; Fedik Abdul Rantam; Suwarno Suwarno; Rahaju Ernawati; Jola Rahmahani
Journal of Applied Veterinary Science And Technology Vol. 4 No. 2 (2023): October 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V4.I2.2023.67-73

Abstract

Background: Avian Paramyxovirus (APMV) type-1 is the leading cause of Newcastle Disease (ND) and taxonomically belongs to the family Paramyxoviridae, genus Avulavirus. Due to its high transmission rate, Newcastle Disease (ND) is included in the A list by the OIE. Purpose: To determine the biological characterize the Newcastle Disease Virus (NDV) field isolate of pigeons (Columba livia) using Mean Death Time (MDT), Intracerebral Pathogenicity Index (ICPI), and Intravenous Pathogenicity Index (IVPI). Methods: Twenty pigeon organ samples were obtained from bird markets in East Java, and one was used as a positive control (LaSota). Organs were isolated from embryonated chicken eggs, identified by the HA test, and then confirmed by the HI test. Furthermore, positive samples were tested for MDT with a 10-1-10-18 dilution (0.1 ml and observed for eight days. The ICPI test used a fresh NDV suspension (0.05 ml and was observed for eight days. The IVPI test used a dose of 0.1 ml and was observed for ten days. Result: The MDT values of isolates MB1/NDV/19, MB2/NDV/19, MB3/NDV/19, and MG1/NDV/19 were 91.2 hours, 112.8 hours, 110.4 hours, and 124,8 hours. The ICPI values of isolate MB1/NDV/19 was 0.2375, MB2/NDV/19 was 0.375, MB3/NDV/19 was 0.5375, and MG1/NDV/19 was 0.3. The IVPI value of isolate MB1/NDV/19 was 0, MB2/NDV/19 was 0, MB3/NDV/19 was 0, and MG1/NDV/19 was 0. Conclusion: All four field samples were positive for NDV as a lentogenic strain based on the MDT, ICPI, and IVPI tests.
Characterization of Gene Coding Fusion Protein of Newcastle Disease Virus Infected in Native Chicken in Surabaya Kirana, Maha; Ernawati, Rahaju; Rahmahani, Jola; Rantam, Fedik Abdul
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.103-108

Abstract

This study aimed to discover the homology of nucleotide sequence, homology percentage, and those relations phylogenetic of protein Fusion (F) gene coding of Newcastle disease in domestic chicken (Gallus gallus domesticus) in Surabaya using some comparison isolate from GenBank. Samples were scoured of digestive organs from native chicken, that was collected from a traditional market in Wonokromo, Surabaya. Samples were tested using RT-PCR with primer forward and reverse with target 976bp, a positive sample which is continued with sequencing then homology and nucleotide analysis which is done and which is translated into amino acid. The result of homology chc/SBY/2018 sample has a similarity of 88% with references sequence, while with Lasota vaccine has a similarity of 87%, and the highest result of homology showed by the comparison with various isolates in Indonesia 90-95%. Translation results from nucleotide alignment into amino acid showed shifts in amino acid structure, which is amino acid shifts could be affected by many things like nutrition, wheater, environment, etc. The conclusion was chc/SBY/2018 sample has a quite high similarity with Indonesian isolates and undergoes mutation on nucleotide structure on amino acid and phylogenetic analysis. This study related to some isolates of vaccine and some isolates in Indonesia.
Effect of Vaccination Outer Membrane Protein 52 kDa on Changes in Erythrocyte Index of Tilapia (Oreochromis niloticus) Infected by Aeromonas hydrophila Nurrahmad, Nanda Rino; Yuliani, M. Gandul Atik; Ernawati, Rahaju; Chusniati, Sri; Herupradoto, Eduardus Bimo Aksono; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28592

Abstract

The purpose of this study was to determine the effect after being vaccinated by OMP 52 kDa Aeromonas hydrophila for 1 week and then infected with Aeromonas hydrophila 10 CFU/mL for 4 days on changes in erythrocyte index. Tilapia (Oreochromis niloticus) used in this study was 10-12 cm long. There were 20 tilapia (Oreochromis niloticus) which were divided into 4 groups, consisting of 2 control groups and 2 treatment groups which were given various types of vaccine formulations. Group P0 (-) (unvaccinated and infected), Group P0 (+) (unvaccinated and infected), group P1 (vaccinated with the whole cell protein "HydroVac®" and infected), and P2 (vaccinated with Outer Membrane Protein 52 kDa and infected) by intramuscular injection. Post-treatment blood samples were collected on day 5 post-infection, collected through a caudal punctie and then analyzed using a hematology analyzer. Post-treatment outcomes led to statistically significant changes (p < 0.05). Therefore, the vaccine caused a significant change in the erythrocyte index.
Co-Authors . Suwarno Adikara, Tatang Santanu Agnes Theresia Soelih Estoepangestie Aksono HP., Eduardus Bimo Boedi Setiawan Christian Marco Hadi Nugroho Chusniati, Sri Didik Handijatno Elisa Herina Dimariwu Fedik Abdul Rantam Gandul Atik Yuliani Hani Plumeriastuti Kartika Aditiya Amelia Kirana, Maha Maria Gladis Bupu Maze Mustofa Helmi Effendi Nurrahmad, Nanda Rino Poedji Hastutiek Prestalia Dwi Rachmawati Rahmahani, Jola Ratih Prajnya Paramitha Setiawan Koesdarto Sunarso, Agus Tyasningsih, Wiwiek Venri Novryantoro Wahyuni, Retno Sri Wiwik Tyasningsih