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All Journal HAYATI Journal of Biosciences Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Jurnal Ilmu dan Kesehatan Hewan Pastura; Journal Of Tropical Forage Science Jurnal Veteriner E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) agriTECH Cakrawala Repositori Imwi Nandur
I Nyoman Dibia
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Veterinary Other

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Journal : Jurnal Veteriner

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Deteksi Virus Avian Influenza Subtipe H5N1 pada Itik di Pasar Hewan Beringkit dan Pasar Galiran, Bali Ni Wayan Intan Martinez; Gusti Ayu Yuniati Kencana; I Nyoman Dibia
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.968 KB) | DOI: 10.19087/jveteriner.2021.22.3.442

Abstract

Avian Influenza merupakan penyakit zoonosis disebabkan oleh virus Avian Influenza (AI) subtipe H5N1, penyakit ini ditemukan hampir di seluruh belahan dunia. Itik adalah unggas air yang merupakan inang alami virus AI. Virus AI subtipe H5N1 yang menyerang unggas dapat ditemukan pada unggas hidup yang dijual di pasar. Pasar hewan berperan penting dalam penyebaran virus AI dari unggas ke unggas, serta dari unggas ke manusia. Tujuan penelitian ini adalah untuk mengetahui sirkulasi virus AI pada itik di Pasar Hewan Beringkit dan Pasar Galiran, Bali. Total sampel 120 swab kloaka dan trakea itik diambil di pasar hewan Beringkit dan Galiran masing-masing 60 sampel. Sampel swab digabung (pooling) berdasarkan pedagang itik dan waktu pengambilan. Isolasi virus dilakukan pada telur ayam bertunas (TAB) berumur sembilan hari. Carian allantois yang dipanen selanjutnya diuji serologis hemaglutinasi (HA/HI) dan uji Reverese Transkriptase Polymerase Chain Reaction. Data hasil penelitian dianalisis secara deskriptif. Hasil penelitian menunjukkan masing-masing satu sampel positif virus AI subtipe H5N1 dari setiap pasar dengan proporsi positif di kedua pasar sebesar 1,7% (2/120). Data ini mengindikasikan bahwa virus AI subtipe H5N1 masih bersirkulasi di Pasar Hewan Beringkit dan Galiran.
Diagnosis and Molecular Marker Analysis of Bali’s Rabies Virus Isolates (DIAGNOSIS DAN ANALISIS PENANDA MOLEKULER VIRUS RABIES ISOLAT BALI) I Nyoman Dibia; Bambang Sumiarto; Heru Susetya; Anak Agung Gde Putra; I Gusti Ngurah Kade Mahardika; Helen Scott-Orr
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.604 KB)

Abstract

The direct fluorescent antibody test (dFAT) was recommended by both World Health Organization(WHO) and Office International des Epizooties (OIE) as a standard diagnostic technique for rabies. Sincethe outbreak of rabies in Bali, it was ascertain the importance to develop a reverse transcriptase-polymerasechain reaction (RT-PCR) technique with specific primers as an alternative diagnostic method. The aim ofthis study was to develop a RT-PCR technique for rabies diagnosis in animals and find out the molecularmarker of Bali’s rabies virus (BRV) isolates based on the sequence of nucleoprotein (N) gene. Brainsamples were obtained during 2009 from 14 suspected rabid dogs and one cattle, where rabies viruseswere isolated. The dFAT was used to detect the presence of rabies viral antigen. Ribonucleic acid (RNA) ofrabies viruses was extracted with TRIzol reagent. Fragment of N gene was amplified using one-step RTPCRmethod with specifically-designed primer pairs and sequenced using ABI automatic sequencer. Multiplealignment of nucleotide and deduced amino acid sequences were analyzed using ClustalW of MEGA 4.0program. This study found that twelve out of fifteen animal brain samples confirmed as rabies by dFAT.Similarly, a single band of 1215 bp PCR product for rabies virus was also detected in twelve out of twelve(100%) dFAT rabies positive samples. It is therefore evident that alternative diagnostic of rabies inanimals can be established using RT-PCR technique. The results showed that the RT-PCR has a very highagreement with dFAT. Polymorphic sites of N gene of twelve BRV isolates were identified at the position186, 501, 801, 840, 1068 and 1153. Bali’s rabies virus isolates have conserved amino acid (isoleucine)alterations at position 308 (open reading frame). Isoleucine distinguished between all Bali’s isolates andthe all of isolates from other area of Indonesia and other part of the world. This finding significantlydifferent as compared to other rabies virus isolates from other part of Indonesia or the world documentedon the GenBank. Accordingly it is proposed that it can be used as molecular marker and believed to be thefirst study of molecular marker of rabies virus in Indonesia.
Faktor-Faktor Risiko Rabies pada Anjing di Bali (RISK FACTORS ANALYSIS FOR RABIES INDOGS IN BALI) I Nyoman Dibia; Bambang Sumiarto; Heru Susetya; Anak Agung Gde Putra; Helen Scott-Orr
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.428 KB)

Abstract

The efforts to eradicate rabies in Bali have been done for more than three years. However, therabiescases is still spreading. Thus, rabies virus continues to infect humans. A case-control study wasconducted to identify the risk factors associated with rabid dog in Bali. Cases were defined as dogsconfirmed having rabies by direct fluorescent antibody test (dFAT). Determination of sample amount ineach district was taken proportionally and samples were taken by using simple random sampling. A totalof 51 rabid dog cases between 2010 and 2011 and 102 uninfected rabies dogs as control were used in thisstudy. Possible associated factors were obtained by doing questionnaire. The data were subsequentlyanalyzed using chi-square (X2) and odds-ratio (OR) for possible association, which were ultimately analyzedby means of logistic regression to build up of model. This study revealed that factors associated with rabiddog were the status of rabies vaccination (X2= 55.538; P= 0.000; OR= 19.133; 95% CI= 8.015<OR<45.678),contact with other dog (X2= 43.659; P= 0.000; OR= 12.551; 95% CI= 5.541<OR<28.430),condition of dog(X2= 9.994; P= 0.002; OR= 3.019; 95% CI= 1.504<OR<6.058),number of raised dog (X2= 9.284; P= 0.002;OR= 2.962; 95% CI= 1.455<OR<6.027), and veterinary care (X2= 5.258; P= 0.022; OR= 2.444; 95% CI=1.125<OR<5.310). It was found an appropriate logit model to estimate probability of rabid dog events inBali province as follows : Logit Pr (rabies=1| x) = - 4.413 + 3.919 (status of rabies vaccination) + 3.457(contact with other dog). This study is expected to be used as a reference in order to improve rabies controleffectiveness in Bali.
Co-Authors Abd. Rahman As-syakur ALDIYO BARUS Anak Agung Gde Putra Bambang Sumiarto CHRIST ALVIN TIMOTHY SIREGAR Gusti Ayu Yuniati Kencana Hapsari Mahatmi Helen Scott-Orr Heru Susetya I DEWA MADE ARTHAGAMA I Gede Oka Darsana I GEDE PADMANABHA I GUSTI AYU ADE TEJASTINI I Gusti Ngurah Kade Mahardika I GUSTI PUTU RATNA ADI I KADEK ANGGA WIRA SENTANA I Ketut Puja I MADE ADI SURYADI I Made Mega I Nengah Netera Subadiyasa I NYOMAN MERIT I NYOMAN RAI I PUTU DHARMA I Wayan Dana Atmaja I Wayan Diara I WAYAN NARKA I Wayan Nuarsa I Wayan Rusna I Wayan Suarna Ida Bagus Suryawan IGNA Wisnu Adi Saputra INDAYATI LANYA Josua Sintong Martin Hutauruk KARINA SAFITRI Ketut Wikantika Made Suci MALINDA NOVIARINI MALINDA NOVIARINI MESI BR HALOHO NI KADEK DIAN ASRI LESTARI Ni Luh Wayan Sayang Telagawathi NI MADE TRIGUNASIH Ni Nengah Soniari Ni Wayan Intan Martinez NOVIN JUWITA CAHYANI NYOMAN SEMADI ANTARA Putu Dimas Abiyoga R. Suyarto REZA ADELIA RIAN REHMAMANA RIO PUTRA NUSANTARA PARDEDE SHERLEYTA PARIPURNANI Suwardhi, Deni Wayan Redi Aryanta WIYANTI WIYANTI Yoga Yuniadi