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All Journal Agrotrop : Journal on Agriculture Science AGRITROP International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Advances in Tropical Biodiversity and Environmental Sciences Nandur
TRISNA AGUNG PHABIOLA
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Veterinary

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Pengaruh Rhizobakteria untuk Memacu Pertumbuhan dan Ketahanan Tanaman Kacang Tanah (Arachis hypogaea L.) terhadap Penyakit Bercak serta Karat Daun PANDE MADE GIOPANY; I MADE SUDANA; TRISNA AGUNG PHABIOLA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.3, Juli 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The Effects of Rhizobacteria to Promote the Growth and Peanut Resistance (Arachis hypogaea L.) Against Leaf Spot and Rust DiseasesPeanut (Arachis hypogaea L.) is an important legume plants that produces seeds with high protein and vegetable fat content, as well as many processed into various food products and as a source of vegetable oil. The purpose of this research is to know the effect of rhizobacteria to promote the growth and resistance of the peanut against diseases. The research used a Randomized Completely Block Design with 5 treatments and 5 replicates. The variables observed were plant height, number of branches, number of leaves, content of leaf chlorophyll, number of root nodules, disease intensity, disease intensity suppression, number of pods per plant, weight of pods per plant, dry weight of seeds per plant, dry weight of seeds per harvested area, and peanut yields per hectare. The results showed that all treatments of rhizobacteria used was able to promote the growth and resistance of the peanut against diseases so that peanut yields could be improved. Rhizobacteria RZ 35 and RZ 36 are rhizobacteria that have the best ability than other rhizobacterias. RZ 35 has more ability in improving growth and peanut resistance against diseases, whereas RZ 36 has more ability in improving peanut yields.
Deteksi Potyvirus pada Gulma Euphorbia heterophylla Bergejala Mosaik di Areal Pertanaman Melon di Kota Denpasar DEBBIE OKTAVIANI DEPARI; TRISNA AGUNG PHABIOLA; GUSTI NGURAH ALIT SUSANTA WIRYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.3, Juli 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Detection of Potyvirus in Mosaic Symptomatic Euphorbia heterophylla in Melon Planting Area in the city of Denpasar Melon (Cucumis melo L.) is a horticultural plant which is included in the Cucurbitaceae group and is highly favored by the community. The development of melon cultivation has faced some obstacles that cause the level of consumption of melon can not be fulfilled. Viruses in plants have a wide range of hosts including several weeds that can be alternative hosts and as a source of inoculums. Potyvirus group is the largest group of plant viruses and plays an important role in causing harm. This research was conducted to determine whether weeds can potentially as an alternative host of Potyvirus so that they can be used as reference materials for academics and farmers so that they take strategies to control weeds in melon plants. Variations in symptoms found in the field include mosaics, vein banding, vein clearing, and chlorosis. The method used in this study was the Polymerase Chain Reaction (PCR). The research activities include (1) sampling and (2) detection and identification of potyviruses in weeds. The results of this study found that Euphorbia heterophylla weeds could become alternative hosts for Potyvirus. The RT-PCR technique succeeded in amplifying target DNA fragments measuring 683 bp according to the CIFOR / CIREV universal primers used in samples from Denpasar.
Identifikasi Virus Penyebab Penyakit Mosaik pada Tanaman Melon (Cucumis melo L.) di Bali RIZWAN FEBIANTA BRAHMANA; TRISNA AGUNG PHABIOLA; I GEDE RAI MAYA TEMAJA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.1, Januari 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Identification of Viruses that Causes Mosaic Diseases in Melons(Cucumis melo L.) in Bali The aims of this research was to identify of some virus that infects melons in Bali. The first step of this research was to take samples in Jembrana Regency, TabananRegency, Gianyar Regency, Denpasar City, and Badung Regency. The next step wasdoing virus detection at Laboratory of Plant Diseases, Faculty of Agriculture, UdayanaUniversity. Variations of symptoms found in the field were mosaic, yellow leaves,malformations, and burning spots on the leaves. The percentage of virus attacks inGianyar and Badung was the highest percentage (60.00%), Jembrana (57.14%),Denpasar (42.85%), and Tabanan (37.50%). To know type of virus that causes thisdisease, molecular identification was conducted. Extraction was the first step to gettotal RNAs from symptomatic leaves. Total RNAs was transcribed intoComplementary (c) DNAs using reverse transctiption-polymerase chain reaction (RTPCR)technique. Polymerase chain reaction (PCR) was conducted using cDNA and three primer pairs of CABYV-489-F/Pol-G-R, MABYV-262-F/Pol-G-R andCIFOR/CIREV. Primers would be use to amplified a part of coat protein. CABYV489-F/Pol-G-Rand MABYV-262-F/Pol-G-R is specific primer, and CIFOR/CIREV would be use to amplified gene of Potyvirus. Based on the results of molecular with PCR it was found that melons in Bali were positively infected with viruses from thePotyvirus group with a size of around 683 bp in samples from Gianyar and Denpasar.
Penentuan Fase Kritis Tanaman Kacang Panjang (Vigna sinensis L.) terhadap Infeksi Bean Common Mosaic Virus (BCMV) NI LUH OCTAVIANI; I MADE SUDANA; TRISNA AGUNG PHABIOLA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Determine of the Critical Phase of Long Bean Plants (Vigna sinensis L.) against Infection of Bean Common Mosaic Virus (BCMV)This study aims to search the critical phase of long bean plants (Vigna sinensis L.) against infection of Bean Common Mosaic Virus (BCMV). The method used is Indirect ELISA (Indirect enzyme-linked immunosorbent assay). This research activity includes variations of symptoms on the long bean plants samples, the incidence of the disease, calculation of yields, the determination of the critical times of the plant, Indirect ELISA serology testing, and data analysis. Design used was a randomized block design (RBD) with seven treatments and four replications.The results showed that the variation of symptoms long bean plants infected BCMV that mosaic, vein banding, malformation of the leaves, stunted and chlorosis. Higher incidence of disease inoculation treatment 2 weeks after planting (WAP) is 91%. Long bean plants are attacked by BCMV the highest yield losses amounted to 93.54% at 2 WAP, so the critical phase of the plant is on the treatment of long bean 2 WAP. Based on these studies need to be done at the beginning of growth and, if already infected plants in early growth, eradication needs to be done so as not to be a source of inoculum.
Pengaruh Aplikasi Formula Pantoea agglomerans Terhadap Aktivitas Antioksidan dan Kandungan Klorofil Daun Tanaman Strowberi TRISNA AGUNG PHABIOLA; KHAMDAN KHALIMI
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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The objectives of this experiment were conducted to evaluate the effectiveness of Pantoeaagglomerans formulations to increase antioxidant activities and chlorophyll content of strawberry plants.P. agglomerans was formulated in the forms of gel and powder. Measurement of peroxidase activitycarried out 7 days after application of P. agglomerans formulations on strawberry plants. The mannerin which the Cohen prosedure suggested by Simon and Rose (1970) and has been modified. Totalchlorophyll content (SPAD unit) was determined with a chlorophyll-meter SPAD-502. Some growthparameters were observed such as plant height, number of leaves, and leaf area. Results of this studyshowed that application of P. agglomerans formulations could increase the peroxidase activity. Peroxidaseactivity on treated plants increased by 47.17% to 50.16% in comparison with un-treated plants. Totalchlorophyll content on treated plants increased by 23.81% to 28.22% in comparison with un-treatedplants. These results suggested that application of P. agglomerans formulations could increase the totalchlorophyll content and antioxidant activity of strawberry plants.
Pengaruh Kehadiran Gulma terhadap Jumlah Populasi Hama Utama Kubis pada Pertanaman Kubis KETUT AYU YULIADHI; TRISNA AGUNG PHABIOLA; MADE SRITAMIN
Agrotrop : Journal on Agriculture Science Vol 3 No 1 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

The Influence of The Presence of Weeds on The Population Number of main Pest of Cabbage on The Cabbage Croppings The aim of the research was to find out the development of population number of Plutella xylostella and Crocidolomia pavonana in the precence and the absence of weeds on the cabbage croppings. The results showed that there were 23 species of weeds in association with plant cabbage, which was made up of 7 species of grasses, 3 species of weeds from Cyperaceae family, and 13 species of broadleaved weeds. Main pests of cabbage on cabbage croppings (Plutella xylostella and Crocidolomia pavonana) was found more on cabbage plants in the absence of weeds compared than that of the presence of weeds.
Population Control of Viruses Insect Vectors in Chili with Plastic Mulch K.A. Yuliadhi; T.A. Phabiola; K. Siadi
Advances in Tropical Biodiversity and Environmental Sciences Vol 1 No 1 (2017): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.837 KB) | DOI: 10.24843/ATBES.2017.v01.i01.p06

Abstract

The incidence of diseases caused by pathogenic viruses in chili is still a major problem in reducing the production of chili in Indonesia. Most agricultural crops are hosts for one or more types of plant viruses, so the viru s continued to be a problem in the tropics. Virus is passive, requires intermediaries vector to be transmitted to other plants. The goal of this research was to develop control strategies for aphids that act as a viral vector and pest chili plants using plastic mulch. Control design that was developed in this study based on the habits of local farmers, using plastic mulch with two colors, black and silver. Mulching is done to dispel the arrival aphids into the chili crop, at the same time preventing the emergence of weeds that act as alternative hosts of the virus. The use of silver plastic mulch to control vector viral populations was better compared to black plastic mulch during  chili planting. The use of silver plastic mulch can improve yields of chili crops.Keywords: Aphid, whiteflies, Chili chili, virus
EFFECT OF BACTERIAL VOLATILE COMPOUNDS ON PAKCOY (Brassica rapa L.) GROWTH PROMOTION Trisna Agung Phabiola; Khamdan Khalimi; Putu Perdana Kusuma Wiguna
International Journal of Biosciences and Biotechnology Vol 9 No 2 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v09.i02.p05

Abstract

This research was aimed to test of the ability of MVOC-producing bacteria to increase plant growth of Pakcoy (Brassica rapa L.). The methodology including testing the ability of MVOC-producing rhizobacteria in Pakcoy plant growth enhancement, MVOC extraction and analysis of compounds in MVOC Extracts using Gas Chromatography. Different bacterial species produce different MVOC. S. maltophilia Sg3 emitted 20 MVOC compounds and MVOC that contribute to increasing plant growth, namely oxalic acid, cyclohexyl undecyl ester, 2-Furancarboxaldehyde, 5-methyl-, 1,2 butanediol, and Piperazine. E. asburiae MjSg48 emitted 12 MVOC compounds and those that contributed to increasing plant growth were oxalic acid, cyclohexyl dodecyl ester and 4-methyl oxazole. E. asburiae TK24 emitted 27 MVOC compounds and those that contributed to increasing plant growth were oxalic acid, isohexyl neopentyl ester, thiazole, Oxalic acid, and cyclohexyl decyl ester. Meanwhile P. rettgeri Al2TT emitted 13 MVOC compounds and those that contributed to increasing plant growth were oxalic acid, diisohexyl ester, and Pyridine, 2,3,4,5-tetrahydro.
Distribution and molecular characterization of Squash mosaic virus on cucumber in Gianyar, Bali Listihani Listihani; Ni Putu Pandawani; Tri Asmira Damayanti; Mimi Sutrawati; Dewa Gede Wiryangga Selangga; Ketut Ayu Yuliadhi; Trisna Agung Phabiola; Gusti Ngurah Alit Susanta Wirya
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 1 (2022): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12248-54

Abstract

Squash mosaic virus (SqMV) has been reported to infect cucumber plants in Java and cause a decrease in fruit quality and quantity. Until now, there is no information regarding Sqmv infection in Cucurbitaceae in Bali. In a preliminary research conducted during 2020, we found mosaic symptoms in Gianyar, Bali. SqMV was inferred to be the possible cause of the virus-disease-like symptoms. The study aimed to determine the precence of SqMV in Gianyar, Bali and to obtain information related to its molecular character. This research method includes surveys, field observations, virus identification by RT-PCR, and DNA analysis. Severe disease incidence caused by SqMV was observed on cucumber cultivation in Gianyar that was in the range of 5.81–66.87%. PCR using specific primer for coat protein (CP) gene of SqMV was successfully amplified the DNA fragments of ± 582 bp on samples from Payangan, Tegallalang, Ubud, Sukawati, Blahbatuh, and Gianyar districts, except for samples from Tampaksiring. This indicates that the presence of SqMV is widespread in Java and Gianyar.The SqMV isolate from Bali had the highest nucleotide homology at 91.9–93.4% and amino acids 94.0–94.5 and was closely related to the Brazil isolate (KT923125) and had lower isolate homology than other countries (China, Trinidad, Spain, Japan, Arizona, Cekoslowakia, and Australia). It proved that those mosaic symptoms on cucumber is associated with SqMV infection. SqMV is classified as a quarantine organism of category A2, so it is necessary to prevent its spread to other areas.
PHYTOCHEMICAL ANALYSIS OF Mucuna pruriens L. LEAVES EXTRACT AND ITS INHIBITION TEST AGAINST Curvularia lunata (WAKK). BOED. THAT CAUSED LETTUCE LEAF SPOT I Made Ary Putra Nata; I Ketut Suada; Trisna Agung Phabiola
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p06

Abstract

Curvularia lunata is a pathogenic fungi that attack various plant species including lettuce. Its control needs to be carried out in an environmentally friendly manner, namely using botanical fungicides. M. pruriens is a legume plant that is widespread in Indonesia that has a potential as a botanical fungicide, beside as a traditional medicine. The purpose of this study was to study the chemical content of M. pruriens leaves and its potency to inhibit the growth of C. lunata. The determination of the compounds was carried out by Gas Chromatography-Mass Spectrophotometry and the inhibition test was carried out using diffusion well, and agar dillution method. The result showed that the crude extract of M. pruriens leaves contained ethyl isothiocyanate that act as an antifungal with AUC of 20,78%. The MIC of the extract was 0,9%. The extract at 5% concentration was capable to inhibit C. lunata growth, mycelium mass, and conidia production respectively 38,33%; 67,14%; 99,88% compared to control.
Co-Authors ANAK AGUNG GDE AGUNG NARENDRA DEBBIE OKTAVIANI DEPARI Dewa Gede Wiryangga Selangga DONI SITUMORANG Gusti Ngurah Alit Susanta Wirya H. Yuswanti HELENA TAMPUBOLON I G. R. M. TEMAJA I GUSTI AYU ARI SANTIKADEWI I GUSTI AYU KARISMAYATI I GUSTI NGURAH PRABU WIRA SANJAYA I KADEK ARYARTHA I Ketut Siadi I Ketut Suada I Made Ary Putra Nata I Made Mega I MADE SUDANA I MADE SUDANA I MADE SUDARMA I MADE TEDI MAHADI PUTRA I MADE WINANTARA I NYOMAN WIJAYA I Putu Sudiarta I PUTU WIRYA SUPUTRA I.G.A. Gunadi Ida Ayu Putri Darmawati Ida Bagus Gde Pranatayana IDA BAGUS GEDE MAHENDRA K.A. Yuliadhi K.B. Susrusa KETUT AYU YULIADHI Ketut Ayu Yuliadhi KEZIA KEZIA Khamdan Khalimi KHAMDAN KHALIMI Listihani, Listihani MADE SRITAMIN Mimi Sutrawati MISBAHUL KHULUQ N.N.A. Mayadewi NI LUH OCTAVIANI NI LUH PUTU NOVITA DEWI NI MADE SAVITA RASJMAN Ni Putu Pandawani NI WAYAN SUNITI PANDE MADE GIOPANY Putu Perdana Kusuma Wiguna Retno Kawuri Rindang Dwiyani RIZWAN FEBIANTA BRAHMANA TRI ASMIRA DAMAYANTI Y. Fitriani