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All Journal Agrotrop : Journal on Agriculture Science AGRITROP International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Advances in Tropical Biodiversity and Environmental Sciences Nandur
TRISNA AGUNG PHABIOLA
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Veterinary

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Tingkat Kerentanan Berbagai Umur Tanaman Melon (Cucumis melo L.) terhadap Infeksi Potyvirus I KADEK ARYARTHA; TRISNA AGUNG PHABIOLA; GUSTI NGURAH ALIT SUSANTA WIRYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Susceptibility Levels of Various Ages of Melon Plants (Cucumis melo L.) Against Potyvirus Infection Domestic melon production has not been able to fulfill the community. Pest disruption is one of the main causes of declining melon production so that the community's demand for melons is not fulfilled. Potyvirus is a virus that is often found in melon plants in Bali, but information about the development of this disease is very limited. This study aims to determine the effect of time on the rate of Potyvirus infection in melon (Cucumis melo L.) plants. The steps taken in this study were preparing plants, inoculating Potyvirus in plants, observing disease progression and conducting research in the laboratory using the RT-PCR (Reverse Transcription Polymerase Chain Reaction) test. The results showed that the younger the melon plants infected by Potyvirus, the faster the rate of infection and the more severe the symptoms caused. The rate of infection in plants treated with inoculation at the age of 2, 3, 4, 5, 6, 7 weeks after planting and without inoculation treatment was 0.96 respectively; 0.60; 0.53; 0.41; 0.30; 0.18 and 0. Melon plants with inoculation treatment of 2, 3 and 4 weeks after planting were very susceptible to Potyvirus infection, which showed signs of severe mosaicism and severe malformations. Melon plants with inoculation treatment 5, 6 and 7 weeks after planting were more resistant to Potyvirus infection, namely showing moderate mosaic symptoms and mild mosaicism, while plants that were not given inoculation treatment showed no symptoms. Detection of Potyvirus using the RT-PCR method using primers (CI-FOR / CI-REV) successfully amplified Potyvirus fragments in the presence of base bands measuring ±683 bp in samples A, B, C, D, E, and F.
Identifikasi Penyebab Penyakit Bercak Merah pada Tanaman Stroberi (Fragaria sp.) di Bali dan Potensi Pengendaliannya dengan Jamur Antagonis Secara In Vitro I GUSTI AYU ARI SANTIKADEWI; GUSTI NGURAH ALIT SUSANTA WIRYA; TRISNA AGUNG PHABIOLA; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Identification of Pathogenic Fungi causes Red Leaf Spot on Strawberry Plants (Fragaria sp.) in Bali and Control Potential with Antagonist Fungi Strawberry (Fragaria sp.) is a subtropical fruit plant that was first discovered in Chile, America and has high economic value. Strawberry plants growing in Bali show symptoms of pathogenic fungi attack. Symptoms include red spots on the leaf surface. The purpose of this study was to identify pathogenic fungi that cause disease in strawberry plants in the center of strawberry cultivation in Bali. The activities carried out in this study were (1) sampling, (2) isolation of pathogenic fungi from symptomatic strawberry plant parts, (3) pathogenicity test, (4) morphological identification of pathogenic fungi, and (5) potential inhibition test of antagonistic fungi. against pathogenic fungi. This research was conducted in Pancasari Village, Buleleng Regency and Candi Kuning and Kembang Mertha Villages, Tabanan Regency and continued at the Plant Disease Laboratory, Faculty of Agriculture, Udayana University, Denpasar. The results of this study succeeded in identifying Pestalotiopsis sp. as a pathogen causingred spot disease on strawberry leaves in Bali and the potential for biological control using antagonistic fungi in vitro showed that Gliocladium sp. and Trichoderma asperellum can suppress the growth of pathogenic fungi on strawberry plants by > 80%, Trichoderma viride up to > 90% and Trichoderma koningii and Trichoderma harzianum up to > 50%.
Metode Isolasi Jamur Patogen Serangga (Aschersonia placenta) Menggunakan Media Water Agar dan Potato Sucrose Agar NI MADE SAVITA RASJMAN; I PUTU SUDIARTA; TRISNA AGUNG PHABIOLA; GUSTI NGURAH ALIT SUSANTA WIRYA; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Isolation Method of Insect Pathogenic Fungi (Aschersonia placenta) using Water Agar Media and Potato Sucrose Agar Bali Province is one of the provinces that is famous for its superior agricultural commodities. One of the leading agricultural commodities is citrus. The production of citrus fruits in Bali Province in 2020 is 4,903,341 quintals based on data from the Central Statistics Agency for Bali Province 2021. However, in some citrus-producing areas, citrus fruit production has decreased compared to the previous year. The decline in citrus fruit production is caused by several factors, one of which is the attack of whitefly pests. Whitefly pests can be controlled by utilizing the insect pathogenic fungus Aschersonia placenta. This study aims to determine the isolation method of insect pathogenic fungi Aschersonia placenta using water agar media and potato sucrose agar. The study began with sampling, isolated the fungus and identifying the fungus by morphological characteristics. The results of this study showed that isolation method of A. placenta using water agar media and potato sucrose agar effective for multiply the fungus.
PELATIHAN AKLIMATISASI BIBIT STROBERI HASIL KULTUR MERISTEM BAGI PETANI STROBERI PANCASARI R. Dwiyani; K.B. Susrusa; I.A.P. Darmawati; G.N.A.S. Wirya; I.G.A. Gunadi; N.N.A. Mayadewi; H. Yuswanti; K.A. Yuliadhi; T.A. Phabiola; R. Kawuri; Y. Fitriani
Buletin Udayana Mengabdi Vol 22 No 3 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i03.p10

Abstract

Abstracts The community service activity entitled "Training for Acclimatization of Strawberry Seeds from Meristem Culture for Pancasari Strawberry Farmers" has been completed. This activity was motivated by the problem of seeds by strawberry farmers in Pancasari, where farmers needed healthy seeds from tissue culture, because through propagation with runners, crop yields decreased drastically due to virus attacks. The solution is the use of seeds from meristem culture. Acclimatization training by farmers is important because removing seeds from culture bottles and then growing them on media ex vitro requires skills and understanding of acclimatization theory. The purpose of this service was to provide strawberry acclimatization skills to farmers, so that farmers can acclimatize themselves to meristem cultured strawberries produced by the Tissue Culture Laboratory of the Faculty of Agriculture Udayana University. This activity was based on the results of strawberry meristem culture research in 2019 funded by the ministry of research technology and higher education Republic of Indonesia through a national competitive grant. The results of the current community service concluded several things as follows: The training participants, especially farmers, have succeeded in carrying out their own acclimatization practice; Strawberry seedlings grown in the highlands (Pancasari) had better vigor than those grown in the lowlands (Denpasar). Keywords: acclimatization, meristem culture, Pancasari, strawberry, virus.
Uji Antagonistik Bakteri Penghasil Indole Acetic Acid Terhadap Jamur Helminthosporium maydis Secara In Vitro HELENA TAMPUBOLON; KHAMDAN KHALIMI; TRISNA AGUNG PHABIOLA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.4, Oktober 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Antagonistic Test of Indole Acetic Acid Producing Bacteria Against Helminthosporium maydis In Vitro Helminthosporium maydis is the cause of important diseases in maize, including leaf blight. Leaf blight is the most dominant disease because it causes large losses. The use of biological agents is one way to treat leaf blight that is environmentally friendly. One of these biological agents is IAA-producing bacteria. This research was conducted in vitro. The results showed that there were several IAA-producing bacteria that were able to inhibit the growth of H. maydis in vitro with the highest inhibition in the treatment of B. thuringiensis GR12 isolates of 96.44% when compared to controls. IAA-producing bacteria were able to inhibit the growth of H. maydis biomass with the percentage of inhibition ranging from 73.97% - 80.82%. The treatment of IAA-producing bacterial filtrate at a concentration of 20%-90% was able to inhibit the growth of H. maydis colonies in vitro. The results of this study indicate that IAA compounds produced by bacteria are antifungal.
Aktivitas Antijamur dari Bakteri Penghasil Senyawa Imidazole-3-Oxide terhadap Jamur Helminthosporium Maydis Trisna Agung Phabiola; Ida Bagus Gde Pranatayana; Khamdan Khalimi
Nandur Vol 4 No 1 (2024)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

The fungus Helminthosporium maydis is a pathogen that causes leaf blight in corn plants.The use of biological agents is one way to control leaf blight that is environmentally friendly. One of these biological agents is bacteria that produce the compound imidazole-3-oxide. This research aims to determine the ability of bacteria producing imidazole-3-oxide compounds to inhibit the growth of H. maydis fungal colonies. The results showed that bacteria producing the compound imidazole-3-oxide were able to inhibit the growth of the fungus H. maydis in vitro with an inhibition percentage ranging from 94.74% to 95.52%. Bacterial filtrate producing imidazole-3-oxide compounds at a concentration of 20% is able to inhibit the growth of the fungus H. maydis with a percentage of inhibitory power ranging from 91.35% to 94.41% whereas at a concentration of 50% it was able to inhibit the growth of the H. maydis fungus with an inhibitory percentage of 100%. The results of this research provide new information that bacteria producing the compound imidazole-3-oxide can be used to control leaf blight disease in corn plants.
Co-Authors ANAK AGUNG GDE AGUNG NARENDRA DEBBIE OKTAVIANI DEPARI Dewa Gede Wiryangga Selangga DONI SITUMORANG Gusti Ngurah Alit Susanta Wirya H. Yuswanti HELENA TAMPUBOLON I G. R. M. TEMAJA I GUSTI AYU ARI SANTIKADEWI I GUSTI AYU KARISMAYATI I GUSTI NGURAH PRABU WIRA SANJAYA I KADEK ARYARTHA I Ketut Siadi I Ketut Suada I Made Ary Putra Nata I Made Mega I MADE SUDANA I MADE SUDANA I MADE SUDARMA I MADE TEDI MAHADI PUTRA I MADE WINANTARA I NYOMAN WIJAYA I Putu Sudiarta I PUTU WIRYA SUPUTRA I.G.A. Gunadi Ida Ayu Putri Darmawati Ida Bagus Gde Pranatayana IDA BAGUS GEDE MAHENDRA K.A. Yuliadhi K.B. Susrusa Ketut Ayu Yuliadhi KETUT AYU YULIADHI KEZIA KEZIA KHAMDAN KHALIMI Khamdan Khalimi Listihani, Listihani MADE SRITAMIN Mimi Sutrawati MISBAHUL KHULUQ N.N.A. Mayadewi NI LUH OCTAVIANI NI LUH PUTU NOVITA DEWI NI MADE SAVITA RASJMAN Ni Putu Pandawani NI WAYAN SUNITI PANDE MADE GIOPANY Putu Perdana Kusuma Wiguna Retno Kawuri Rindang Dwiyani RIZWAN FEBIANTA BRAHMANA TRI ASMIRA DAMAYANTI Y. Fitriani