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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Entomologi Indonesia Advances in Tropical Biodiversity and Environmental Sciences Berkala Penelitian Hayati Nandur
MADE SRITAMIN
Program Studi Agroekoteknologi Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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PENGENDALIAN HAMA DAN PENYAKIT TANAMAN PADI DI DESA PESABAN, KECAMATAN RENDANG, KARANGASEM I M. Sudarma; N. M. Sritamin; I G.N. Bagus
Buletin Udayana Mengabdi Vol 15 No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Tujuan dari pengabdian ini adalah untuk meningkatkan pengetahuan petani tentang cara pengendalian hamadan penyakit tanaman padi di Desa Pesaban, Kecamatan Rendang Karangasem. Metode dari pengabdianmasyarakat ini adalah penyuluhan dan wawacara langsung kepada petani setempat. Kesimpulan dari kegiatanpengabdian masyarakat ini adalah pelaksanaan kegiatan berlangsung dengan sangat lancar dan terjadi diskusidua arah dari peserta aktif dengan pertanyaan dan respon terhadap topik yang diberikan. Kagiatan inidirasakan sangat berguna bagi masyarakat petani setempat, mengingat petani dengan permasalahan yangditemukan di sawah, perlu mendapat penanganan yang serius demi keselamatan usahatani tanaman padi.
HAMA DAN PENYAKIT PADA TANAMAN JERUK SERTA PENGENDALIANNYA I N. Wijaya; W. Adiartayasa; I G.P. Wirawan; M. Sritamin; M. Puspawati; I M. Sudarma
Buletin Udayana Mengabdi Vol 16 No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Sebagian besar penduduk Desa Katung, Kecamatan Kintamani, Kabupaten Bangli sebagai petani jeruk yang tergabung dalam kelompok Subak Abian Kerta Winangun. Pertanaman jeruk di daerah ini sangat berhasil, sehingga pendapatannya meningkat. Namun belakangan, petani resah karena tanaman jeruknya terserang hama dan penyakit terutama penyakit CVPD (Citrus Vein Phloem Degeneration) dan hama lalat buah. Oleh karena itu perlu diadakan penyuluhan dan pelatihan tentang pengelolaan hama dan penyakit pada tanaman jeruk. Adapun tujuan penyuluhan dan pelatihan ini untuk memberikan pengetahuan dan keterampilan kepada para petani setempat tentang bahaya serangan hama dan penyakit serta pengendaliannya pada tanaman jeruk meliputi : gejala serangan, faktor-faktor yang mempengaruhi perkembangan hama dan penyakit, cara penularan penyakit serta cara pengendaliannya. Metode yang digunakan dalam pelatihan ini adalah penyampaian materi di ruangan dan praktek di perkebunan jeruk. Adapun tujuan penyuluhan dan pelatihan ini untuk memberikan pengetahuan dan keterampilan kepada para petani setempat tentang bahaya serangan hama dan penyakit serta pengendaliannya pada tanaman jeruk meliputi : gejala serangan, faktor-faktor yang mempengaruhi perkembangan hama dan penyakit, cara penularan penyakit serta cara pengendaliannya. Pelatihan ini dipandu oleh 5 (lima) orang pelatih yang berasal dari Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana. Pelatihan diikuti diikuti oleh 50 orang peserta. Penceramah memaparkan materinya dengan menggunakan alat peraga berupa power point dan menggunakan spesimen berupa gejala serangan hama dan penyakit. Kemudian dilanjutkan dengan praktek lapangan selama 60 menit. Masing-masing peserta diberi tugas untuk mencari tanaman jeruk yang terserang hama dan penyakit serta penyebabnya. Dalam pelaksanaan pelatihan tersebut, petani sangat antusias untuk mengikuti penyuluhan. Hal ini terbukti dari banyaknya pertanyaan-pertanyaan yang diajukan. Untuk mengetahui keberhasilan kegiatan ini, maka dilaksanakan evaluasi dengan pengisian kuisioner sebelum dan sesudah pelatihan.
AWAS BAHAYA PENYAKIT CVPD DAN TEKNIK PENGENDALIANNYA PADA TANAMAN JERUK I. N. Wijaya; M Sritamin; W Adiartayasa; I. G. N Bagus; M Sudarma; N. M. Puspawati
Buletin Udayana Mengabdi Vol 13 No 2 (2014): Vol 13, No. 2 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Education and training on integrated pest management of CVPD (Citrus Vein Phloem Degeneration) was held at Sengkaduan,Taro village, Gianyar regency on 14 August 2014. The activities aimed to improve the farmers’ knowledge of CVPD and integrated pest management. The training was attended by 44 participants from local farmer groups of Sengkaduan village. The method used were lectures, demonstration and practice in the citrus field. All participants participated enthusiastically and hope they have the next intensive training of the citrus culture.Keywords : Training, CVPD, Sengkaduan
Uji Efektivitas Bahan Hayati Dari Cacing Tanah (Lumbricus Rubellus) Terhadap Perkembangan Populasi Nematoda Puru Akar (Meloidogyne Spp.) Pada Tanaman Tomat Varietas Karina (Lycopersicum Esculentum Mill.) SUNARTI TAMBUNAN; MADE SRITAMIN; I DEWA PUTU SINGARSA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.3, Juli 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT The Effectiveness Test of Biological Materials from the Earthworm (Lumbricus rubellus) to the Development of Root Knot Nematodes Population (Meloidogyne spp.) on the Tomato Crops of Karina Variety (Lycopersicum esculentum Mill.)   Tomato (Lycopersicum esculentum Mill.) is one of the agricultural commodities with high economic value and widely consumed both as a vegetable and spice, and is often used as fresh fruit and healthy beverage ingredients. The aim of this study was to determine the ability of biological materials of earthworms (L. rubellus) to suppress the development of root knot nematode populations (Meloidogyne spp.), and the level of effectiveness in suppressing the development of Meloidogyne spp. This treatment consists of 5 different types of biological material treatment, namely the earthworm L. rubellus (10 birds per adult earthworms plants), fresh extract of L. rubellus (at a dose of 100 cc per plant), manure (compost) L. rubellus (dose of 100 cc per plant), capsules L. rubellus (dose of 100 cc per plant), and urine L. rubellus (dose of 100 cc per plant). The results showed that treatment using biological material from dung (vermicompost) of earthworm L. rubellus was the most effective in suppressing the development of root knot nematode population of Meloidogyne spp. in 300 g of soil that is 12 earthworms/300 g soil with an emphasis percentage of 97.6%. In the calculation of the nematode population Meloidogyne spp. per 1 g of roots, the results showed that treatment with biological material of adult earthworms L rubellus was the most effective in suppressing the development of root knot nematode population of Meloidogyne spp. ie 35 earthworms / 1 g of roots with an emphasis percentage of 93%.   Keywords: Lumbricus rubellus, Lycopersicum esculentum Mill., and Meloidogyne spp.
Uji Efektivitas Beberapa Ekstrak Daun Tanaman terhadap Populasi Ulat Daun Kubis (Plutella xylostella L.) pada Tanaman Kubis di Lapang NI KADEK BUDARTINI; KETUT AYU YULIADHI; MADE SRITAMIN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.3, Juli 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Effectivity of Some Extract to Diamonback Moth (Plutella xylostella) Population On Cabbage PlantThe research was conducted in November 2017 to February 2018 at Candikuning Village, Baturiti District, Tabanan Regency. The aim of this research is to observe effectiveness some extract on larvae of Diamonback Moth (Plutella xylostella) population on cabbage plant. Plant extract materials used in this experiment were leaf of Chromolaena odorata, Lantana camara, Tithonia diversifolia and Nicotiana tabacum. Process of extracting at Laboratory of Genetic Resources and Molecular Biology Faculty of Agriculture Udayana University. This research uses Randomized Block Design (RBD) with 4 treatment of leaf extract at 10% concentration and 1 without treatment (kontrol), there treatments was replicate 5 times. Observations were made on cabbage plants one week after planting and before application. Subsequent observations were conducted weekly, up to harvest by calculating the population larvae of Plutella xylostella. Testing of four types of plant leaf extracts showed the following results: Each of C. odorata leaf extract, L. camara, T. diversifolia, and N. tabacum used as research material had different potential in suppressing P. xylostella populations . Application of C. odorata and T. diversifolia leaf extracts was able to suppress P. xylostella populations faster than other treatments. The weight of cabbage crop on treatment of C. odorata was higher than that of other treatments and the quality of crop in all four types of extract showed quality category 2.
Uji Efektifitas Ekstrak Daun dari Beberapa Jenis Tanaman untuk Mengendalikan Nematoda Puru Akar Meloidogyne Spp. pada Tanaman Cabai (Capsicum Annum L) IDA BAGUS MADE DWI JAYA; MADE SRITAMIN; NI MADE PUSPAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.2, April 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Root knot nematodes (Meloidogyne spp.) is an important class of nematode that causes significant reduction in the production of Capsicum annum L. Various control measures were taken to suppress the population of Meloidogyne spp. such as planting resistant varieties, crop rotation and technical culture, still unwell provide fast response compared to chemical control. Biopesticides is a way of controlling the use of more environmentally friendly than synthetic pesticides. Based on this, it will be carried out studies on the control of root knot nematodes Meloidogyne spp. using plant leaves tembelekan (Lantana camara), kirinyuh (Chromolaena odorata), papaya (Carica papaya), lemongrass (Cymbopogon nardus L.), betel (Piper betle L.) and tobacco (Nicotiana tabacum) which extracts used were 250cc / pots of each extract solution leaves. The purpose of this study was to determine the plant leaf extract is capable of suppressing the population of Meloidogyne spp. and determine the most effective leaf extracts suppress the population of Meloidogyne spp. The results of this empirically found that population of nematoda per 300 grams of soil extract of Piper betle L. can suppressed the nematode population of Meloidogyne spp. The best is 23.4 nematodes or 95.32%, followed by treatment of the extract of C. odorata 27.6 nematodes (94.48%), L. camara 28.8 nematodes (94.24%), Carica papaya 43, 4 nematodes (91.32%), Cymbopogon nardus L. 53.8 nematodes (89.4%), and Nicotiana tabacum 60.8 nematodes (87.84%). Population of nematoda per 1 g of root, extract of Piper betle Linn. is also the most well extract in suppressing root knot nematode populations, there are only recorded 21.2 percentage suppression tail with 95.76%, followed by treatment of C. odorata extract 23.6 tail (95.28%), L. camara 24 , 2 goats (95.16%), Carica papaya 28.4 tail (94.32%), Cymbopogon nardus (L). 30.2 tail (93.96%), and Nicotiana tabacum 35.2 tail (92.96%). Keyword: Capsicum annum L, Carica papaya, Chromolaena odorata, Cymbopogon nardus L., Lantana camara, Meloidogyne spp., Nicotiana tabacum, Piper betle Linn.
Kultur In vitro Tanaman Jeruk Pamelo (Citrus maxima Merr.) Menggunakan explant Biji Serta Deteksi Keberadaan Bakteri Liberobacter asiaticus pada Kalus yang Dihasilkan PUTU BAMBANG IKA WINJALISTA; I GEDE PUTU WIRAWAN; MADE SRITAMIN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.2, April 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

In vitro culture of citrus plant Pamelo (Citrus maxima Merr.) Using Explant seeds as well as the detection of the presence of Bacteria Liberobacter asiaticus at Shoots are produced This research was begun with doing sample approachment in Kapal Village, Mengwi Sub-District, Badung regency and Sumerta Klod Village, East Denpasar Sub-District, Denpasar City and the culture in vitro was done in Implementing Technical Unit (UPT) Sumberdaya Genetika dan Biologi Molekuler Laboratory since October 2018 until January 2019. This research aim to recognize the response of pamelo orange's seed explant rather it will grow in based MS media which has been richen and got pamelo orange seed that is free from the infection of liberobcter asiaticus. The approachment of sample was based on the visualization of the plant, the plant which shows any indication of CVPD disease next will be known as 1st Sample and 2nd sample would be the plant which shows no indication of cvpd disease. The seed of each sample be cultured with MS media which has been richen, was maintained for 8 weeks after it's planted (mst). By the results of this research, it concludes that there's seed explant from both samples which grows without showing any indication of cvpd disease, but the result of DNA amplification from 1st sample that has been cultured in vitro after got UV transluminator visualization shows the existence of 1160 bp DNA ribbon which is belong to Liberobcter asiaticus. This shows that the sample has been infected by Liberobacter asiaticus, whereas can't be found in 2nd sample.
Deteksi Penyakit Tanaman Cabai Rawit (Capsicum frutescens L.) yang Tumbuh Disekitar Tanaman Jeruk Bergejala Citrus Vein Phloem Degeneration (CVPD) Menggunakan Teknik Polymerase Chain Reaction (PCR) NI KADEK DWI PASARI; I GEDE PUTU WIRAWAN; MADE SRITAMIN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.1, Januari 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Detection of Chilli plant (Capsicum frutescens L.) which Grow in the Area of Citrus Cultivation that had symptoms of Citrus Vein Phloem Degeneration (CVPD) Using Polymerase Chain Reaction (PCR) Technique Liberibacter bacteria live and thrive inside the phloem tissue CVPD affected plants, the bacteria exhibit a progressive degeneration of the phloem tissue that inhibit phloem nutrient transport through the entire plan.. As the attack of Liberibacterasiaticus cause Citrus Vein Phloem Degeneration (CVPD) disease in citrus plant, it is necessary to do research on the chilli plant around citrus plant area to know whether bacteria of Liberibacter also attack the chilli plant by using PCR.The results of this study indicate that the chilli plants with CVPD symptoms which are suspected to be caused by the bacterium Liberibacter by usingLiberibacter asiaticus primer and Liberibacter solanaceae which is them specific primer of 16S rDNA are not proven. The result showed chilli plants with similar symptom to CVPD in Mangguh village, Kintamani sub-district were not to be caused by Liberibacter asiaticus and Liberibactersolanaceae.
Organogenesis Tanaman Bawang Merah (Allium ascalonicum L.) Menggunakan Umbi Secara In Vitro pada Media Dasar Murashige and Skoog yang Diperkaya Vitamin B5 dengan Naftalene Acetic Acid dan 6-Benzyl Amino Purine KATARINA IKA NOVIANITA; MADE SRITAMIN; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.3, Juli 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Organogenesis of Shallot Plant (Allium ascalonicum L.) Using Shallot Bulb with In Vitro Method on Murashige and Skoog Basic Media that Enriched by Vitamin B5 with Naftalene Acetic Acid and 6-Benzyl Amino Purine This study was conducted to determine the effect of the combination of NAA (Naftalene Acetic Acid) and BAP (6-Benzyl Amino Purine) in MS media which can stimulate organogenesis in the onion (Allium ascalonicum L.) Biru Lancor variety in vitro. This experiment used four treatments, namely P1 (NAA 1 mg / L + BAP 2 mg / L), P2 (NAA 2 mg / L + BAP 2 mg / L), P3 (Vitamin B5 + NAA 1 mg / L + BAP 1) mg / L), and P4 (Vitamin B5 + NAA 2 mg / L + BAP 2 mg / L). Based on observations made on the growth and development of onion bulbs in vitro, treatment P3 with the combination of concentration 1 mg / L NAA and 1 mg/L BAP with the addition of vitamin B5 can stimulate organogenesis well to the number of leaf shoots, leaf length, number of roots and root length. This media provides the best results for the observed parameters compared to other treatments.
Induksi Mutasi Kromosom dengan Kolkisin pada Tanaman Anggur Merah (Vitis vinifera L. Varietas Prabu Bestari) Melalui Pembentukan Kalus Secara In Vitro DIMINDA ELA SRI ERTINA BR GINTING; MADE SRITAMIN; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Induction of Chromosome Mutations with Colchicin in Red Grape (Vitis vinifera L. Prabu Bestari Varieties) Through In Vitro Callus Formation This study aims to determine the colchicine concentration that can induce chromosome mutations in red grape, carried out in vitro. This study used a completely randomized design (CRD) with 9 treatments and 3 replications so that the number of experimental units was 27 bottles of culture. The sterilized red grape node explants were immersed in colchicine at the concentration according to the treatment for 24 hours. The colchicine concentration used was 0.015% (K1), 0.020% (K2), 0.025% (K3), 0.030% (K4), 0.035% (K5), 0.040% (K6), 0.045% (K7), 0.050% ( K8) and 0% (K0) as controls. The effect of immersion in several colchicine concentrations on chromosome mutations in explants of red grape nodes that form callus cannot be observed because the chromosomes are not clearly visible, and the number of chromosomes cannot be calculated. Callus was formed in colchicine immersion treatment with a concentration of 0.015% (K1) and 0.035% (K5). Callus on K5 was formed faster, namely 10 weeks after planting, while K1 was at 11 weeks after planting. The average callus diameter on K5 was larger with a size of 4.3 mm, while on K1 it was 2 mm.
Co-Authors Abd. Rasyid Syamsuri Ade Febrianto Umbu Romu Kolambani ANAK AGUNG GEDE GARBA YOGANTARA Aulanni ‘am Aulanni ‘am BAYU ARYA BAGASKARA CATUR KOESUMA WARDHIANY DARWIN SILALAHI DELVIANA PANJAITAN DEWA AYU RATIH DIMINDA ELA SRI ERTINA BR GINTING EKA BHUWANA BONAULI BARITA Evy Prima Fransen Sinaga Gresia Eldina GUSTI PUTU DINTYA PUTRA Haryadi, Celvin Hengky Surya Bhuana I DEWA PUTU SINGARSA I G. N. Bagus I G. Ngurah Bagus I G.N. Bagus I Gede Ketut Susrama I GEDE PUTU WIRAWAN I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Suada I KETUT SUMIARTHA I M. Sudarma I Made Mega Adnyana I N. Wijaya I N. WIJAYA I NYOMAN TRYADI CAHYA NUGRAHA I NYOMAN WIJAYA I Putu Eko Arymanto Wira Kesuma I. G. N Bagus I. N. Wijaya IDA BAGUS MADE DWI JAYA KATARINA IKA NOVIANITA KETUT AYU YULIADHI Liliek Sulistyowati LUH PUTU SUGIARI M Sudarma M. Puspawati Made Adnyana MAGNA DWIPAYANA MUSLIM MUSLIM NI KADEK BUDARTINI NI KADEK DWI PASARI Ni Kadek Emi Sintha Dewi Ni Kadek Sintya Dewi NI KADEK SINTYA DEWI NI LUH MADE PRADNYAWATHI Ni Made Puspawati NI MADE PUTRI YUDANTARI Nona Au Waling NURUL KHOTIMAH PUTU ANA DIANTARI PUTU AYU MEITA YUDIA DEWI PUTU BAMBANG IKA WINJALISTA PUTU PADMA MAHESWARI Rindang Dwiyani RISMA IMROATUS SHOLIHAH Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sasadara, Maria Malida Vernandes Shara Yulita Harianja SHEILA SIMANJUNTAK SUNARTI TAMBUNAN TRIAN WANGSA PURWANTO TRISNA AGUNG PHABIOLA VLORA VERONICA SIPANGKAR WAYAN ADIARTAYASA Wijaya I N. Yundari, Yundari