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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Entomologi Indonesia Advances in Tropical Biodiversity and Environmental Sciences Berkala Penelitian Hayati Nandur
MADE SRITAMIN
Program Studi Agroekoteknologi Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Pembentukan Mutan Hipovirulen Fusarium oxysporum f.sp cubense Penyebab Layu pada Tanaman Pisang (Musa paradisiaca L.) Menggunakan Asam Nitrat Fransen Sinaga; Made Sritamin; I Ketut Suada
Nandur Vol 1 No 1 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

The Formation of Fusarium oxysporum f.sp. cubense Hypovirulent Mutant Causes Wilt in Banana Plants (Musa Paradisiaca L.) Using Nitric Acid. Fusarium wilt disease is a type of disease that can cause a quantitative decrease in banana plant production. Fusarium wilt disease is classified as a disease that is difficult to control, because it is underground and forms chlamydospores. Control of Fusarium with biological agents has a positive impact on the environment, one way of biological control using antagonistic microorganisms. Biological agents used are biological agents that are hypovirulent. Hypovirulent properties will compete with host pathogens in competition for space, food, and toxin excretion, thereby suppressing fusarium fungi to enter the vascular tissue of banana plants. Chemical mutagens are mutagenic agents that can cause permanent changes in the composition of DNA. Nitric acid can cause DNA cross-linking in the same strands. Nitric acid was reported as a suitable mutagen to increase the production of lactic acid Lactobacillus delbrueckii. Nitric acid can change the acid-base pair of organisms so that mutations occur. obtained a mutant that is hypovirulent which can suppress the growth of fusarium fungus as a pathogen in banana plants. The results showed that the FOC fungus isolates with 1% treatment had hypovirulent properties compared to 0.05%, 0.1% and 0.5% treatments. Hypovirulent properties are derived from the timing of symptoms. In mutant FOC, 1% of symptoms appeared on day 6. The growth of hypovirulent mutants was expected to be higher than that of pathogenic FOCs. Based on the research, it was found that the fungi had the lowest pathogenicity level, as well as high diameter growth, total spore density, and high amount of inhibition.
Identifikasi Senyawa Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Etanol Limbah Kulit Biji Kakao (Theobroma cacao L.) Varietas Trinitario Haryadi, Celvin; Wirawan, I Gede Putu; Sritamin, Made
Agrotrop : Journal on Agriculture Science Vol 13 No 3 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i03.p03

Abstract

Identification of Phytochemical Compounds and Antioxidant Activity Test of Ethanol Extract of Cocoa Bean Shell Waste (Theobroma cacao L.) Trinitario Varieties. Cocoa is one of the mainstay commodities of plantations in Indonesia, contributing to the country's foreign exchange earnings. Byproducts such as fruit skins and pulp are produced during the production of dry cocoa beans, and waste in the form of cocoa bean husks is produced during the processing of dry cocoa beans into chocolate products. So far, cocoa bean husk waste has not been used optimally, leading to a low economic value. The purpose of this study was to determine the bioactive compounds in the skin and the levels of antioxidant compounds in cocoa bean husk waste so that it could be used as a raw material for medicine. The GC-MS analysis method was used to identify bioactive compounds in cocoa bean husk waste, and the DPPH method was used to test antioxidant activity. According to the results of the GC-MS analysis, the cocoa bean husk waste extract contains four compounds with quality value higher than 90. These four compounds are Alpha-Copaene, Copaene, Caryophyllene, and Caffeine. The antioxidant test results of the cocoa bean husk waste extract revealed an IC50 value of 86,213 ppm, indicating strong antioxidant activity because the IC50 value is between 50-100 ppm.
Co-Authors Abd. Rasyid Syamsuri Ade Febrianto Umbu Romu Kolambani ANAK AGUNG GEDE GARBA YOGANTARA Aulanni ‘am Aulanni ‘am BAYU ARYA BAGASKARA CATUR KOESUMA WARDHIANY DARWIN SILALAHI DELVIANA PANJAITAN DEWA AYU RATIH DIMINDA ELA SRI ERTINA BR GINTING EKA BHUWANA BONAULI BARITA Evy Prima Fransen Sinaga Gresia Eldina GUSTI PUTU DINTYA PUTRA Haryadi, Celvin Hengky Surya Bhuana I DEWA PUTU SINGARSA I G. N. Bagus I G. Ngurah Bagus I G.N. Bagus I Gede Ketut Susrama I GEDE PUTU WIRAWAN I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Suada I KETUT SUMIARTHA I M. Sudarma I Made Mega Adnyana I N. WIJAYA I N. Wijaya I NYOMAN TRYADI CAHYA NUGRAHA I NYOMAN WIJAYA I Putu Eko Arymanto Wira Kesuma I. G. N Bagus I. N. Wijaya IDA BAGUS MADE DWI JAYA KATARINA IKA NOVIANITA KETUT AYU YULIADHI Liliek Sulistyowati LUH PUTU SUGIARI M Sudarma M. Puspawati Made Adnyana MAGNA DWIPAYANA MUSLIM MUSLIM NI KADEK BUDARTINI NI KADEK DWI PASARI Ni Kadek Emi Sintha Dewi Ni Kadek Sintya Dewi NI KADEK SINTYA DEWI NI LUH MADE PRADNYAWATHI Ni Made Puspawati NI MADE PUTRI YUDANTARI Nona Au Waling NURUL KHOTIMAH PUTU ANA DIANTARI PUTU AYU MEITA YUDIA DEWI PUTU BAMBANG IKA WINJALISTA PUTU PADMA MAHESWARI Rindang Dwiyani RISMA IMROATUS SHOLIHAH Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sasadara, Maria Malida Vernandes Shara Yulita Harianja SHEILA SIMANJUNTAK SUNARTI TAMBUNAN TRIAN WANGSA PURWANTO TRISNA AGUNG PHABIOLA VLORA VERONICA SIPANGKAR WAYAN ADIARTAYASA Wijaya I N. Yundari, Yundari