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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Entomologi Indonesia Advances in Tropical Biodiversity and Environmental Sciences Berkala Penelitian Hayati Nandur

MADE SRITAMIN

Program Studi Agroekoteknologi Pertanian Universitas Udayana

Author-ID : 2952007

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Perbanyakan Tanaman Jeruk Siam (Citrus nobilis L.) dengan Teknik Kultur in vitro Menggunakan Biji Tanaman Terinfeksi Penyakit Citrus Vein Phloem Degeneration (CVPD) BAYU ARYA BAGASKARA; I GEDE PUTU WIRAWAN; MADE SRITAMIN; I GUSTI AYU DIAH YUNITI
Agrotrop : Journal on Agriculture Science Vol 8 No 2 (2018)
Publisher : Fakultas Pertanian Universitas Udayana

Citrus (Citrus nobilis L.) Propagation Through Culture In Vitro Using Seed From Infected Plants Of Citrus Vein Phloem Degeneration (CVPD) Disease. The sample was taken in Belancan Village, Kintamani District, Bangli Regency, and invitro culture was conducted in UPT. Genetics Resource and Molecular Biology Laboratory in February to June 2018. This research purposes were to get the new citrus plant from citrus seed infected of CVPD disease explants with culture in vitro and found the presence of the bacterium Liberobacter asiaticus in results of citrus plants culture in vitro. The sampling method is using plants appearance. Obtained sample 1 is a plant that shows severe symptoms of CVPD disease and sample 2 plants that show no symptoms of CVPD disease. Seed Sample is cultured with MS0 medium for 8 WAP then proceed to acclimatization. The results of this research showed that the seed explants from both samples were able to grow well and did not have the characteristic of CVPD disease, but explant seed sample 2 in vitro culture result showed that the bacterial DNA bacterium Liberobacter asiaticus pathogen CVPD disease does exist in length 1160bp, so it cannot be expected to produce CVPD-free crops.

Transformasi Genetik Tanaman Kentang (Solanum tuberosum L.) dengan Gen acvB Menggunakan Vektor Agrobacterium tumefaciens DARWIN SILALAHI; I GEDE PUTU WIRAWAN; MADE SRITAMIN
Agrotrop : Journal on Agriculture Science Vol 11 No 1 (2021)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2021.v11.i01.p07

Agrobacterium tumefaciens Mediated Genetic Transformation of acvB Gene in Potato (Solanum tuberosum L.). Genetic transformations are now routinely applied to plant mediated by Agrobacterium tumefaciens as the most convenient technique. This study aimed to prove the success of A. tumefaciens mediated genetic transformation in potato. A. tumefaciens LBA (pBI 121) and explant of potato shoot were used in this study. Explants were grown in vitro on Murashige and Skoog media. Transformation was implemented using smear technique by smearing A. tumefaciens to injured explant. Experimental groups consisted of two groups: control group which did not receive transformation treatment and treatment group receiving transformation treatment. Explant growth was observed through the presence of shoots, branches and the shoot height. Explants in the treatment group resulted in a higher number of shoots, branches, and shoot heights compared to control. Phenol compounds appear in explant epidermal tissue, indicating the wounds produced by A. tumefaciens infection, thus the gene predicted to be transformed. Identification by PCR is needed to prove the existence of the acvB gene in potato plants genome, using acvB specific PCR primer as the marker, such as (5?-CCCT CTAG AGAC CCGC GCCA AGGCG-3?) and (5?CGCG TCGA CCTT GTCG GAAAG -3?) with 540-bp in base pair size produced.

Kultur Jaringan Jeruk Keprok Tejakula (Citrus reticulata var. Tejakula) Menggunakan Tunas Muda dan Biji Serta Deteksi CVPD dengan Teknik Polymerase Chain Reaction (PCR) VLORA VERONICA SIPANGKAR; I NYOMAN WIJAYA; MADE SRITAMIN
Agrotrop : Journal on Agriculture Science Vol 10 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Udayana

Tissue Culture Citrus (Citrus reticulata var. Tejakula) using Shoot and Seed and the Detection of CVPD with Polymerase Chain Reaction (PCR). The sample was taken in Pecatu Village, Kuta Selatan District, Badung Regency and continued with in vitro culture and PCR analysis at UPT. Genetic Resource and Molecular Biology Laboratory, was start from December of 2018 until May of 2019. The purpose of this research was to create a CVPD free Citrus reticulata var. Tejakula seedling from tissue culture. The sample was chosen based on visual characteristic. Two samples were taken from citrus plant that show CVPD symptoms and citrus plant that did not show CVPD symptoms. The seed and shoot were cultured using MS media for 12 week after planting (WAP). From this research showed that both explant from the shoot and seed were able to grow well. The explant from citrus sample that showed CVPD symptoms was infected by Liberibacter asiaticus and the explants from citrus that did not show CVPD symptoms was not invected by Liberibacter asiaticus, according to PCR analysis.

IMMUNOGENESITY OF SPESIFIC PROTEIN MOLECULAR WEIGHT 16 KDa (PS16) LEAF OF SIAM CITRUS INFECTED BY CITRUS VEIN PHLOEM DEGENERATION (CVPD) DISEASE Made Sritamin; Aulanni ‘am Aulanni ‘am; IGP. Wirawan; Liliek Sulistyowati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/325

Citrus Vein Phloem degeneration (CVPD) is an important citrus disese, which damaged citrus plantation and causing decrease of citrus production. In Indonesia, the CVPD disease caused by Liberobacter asiaticum bactery and the disease spread out by vectir insect Diaphorina citri and using infected bud in wood grafting. In infected citrus plant, two specific protein molecules with molecular weigt 16 kDa and 66 kDa are found. These protein molecules are not found in healthy citrus plant. The immunogenicity of PS16 accumulated on leaf of citrus plant infected by CVPD is known yet. The research material were leaves of citrus plant infected CVPD, leaves of healthy citrus plant and reagent used these research are for isolation of the total protein leaf of citrus plant, SDS-PAGE electroforesis, electroelution of PS16, ELISA Methods, Dot-Blot Method, anti-PS16 as aprimery antibody and secondary antibody is anti-Rabbit IgG Conjugated AP. The result of the research showed that of PS16 accumulated on leaf of citrus plant infected CVPD has immunogenic character. It is indicated by increase of the titer anti-PS16 after first immunization ang 2nd booster by indirect ELISA method and can be used to induce antibody (anti-PS16) and so showed that positive reaction between PS16 with anti-PS16. It is indicated by purples dark blue on cellulose membrane by Dot Blot method.

Dinamika Populasi Diaphorina citri Kuwayama (Homoptera: Psyllidae) dan Deteksi CVPD dengan Teknik PCR I NYOMAN WIJAYA; WAYAN ADIARTAYASA; MADE SRITAMIN; KETUT AYU YULIADHI
Jurnal Entomologi Indonesia Vol 7 No 2 (2010): September
Publisher : Perhimpunan Entomologi Indonesia

The research was conducted in the field of Taro village Gianyar regency and Katung village Bangli regency and at the Laboratory of Biotechnology Faculty of Agricultural Udayana University. The purpose of the research were to investigate population dynamic of Diaphorina citri Kuwayama and other insects that were assosiated with citrus cv. siam; and molecular detection of CVPD patogen in its vector, other phytophagous insects on citrus cv.siam and kemuning [Murraya paniculata (L.) Jack.] The result of this study showed that in six month period there was one peak of D. citri population which was associated with the flushing period of citrus at Taro as well Katung villages. The peak of D. citri population of Taro village was higher than that of Katung village. Two species of parasitoids and four spesies of predators were found in the field and may contribute in the suppression of D. citri population. The parasitoids identified were Tamarixia radiata Wat. and Diaphorencyrtus alligharensi Shaffe, whereas the predators were Curinus coeruleus Mulsant, Phiddipus sp., Menochilus sexmaculatus Fabricius and Oxyopes javanus Thorell. T. radiata has been proven to be an effective nymph parasitoid that could suppress the population development of D. citri in citrus orchads. The research revealed that kemuning could serve as a source of infection because it is also a host of CVPD disease as well as of D. citri. Toxoptera citricidus Kirkaldy and Maleuterpes dentipes Hell. were not proven to contain CVPD disease.

Utilization of Betel Leaf Extract as Botanical Pesticides to Control meloidogyne spp. and Tomato Plant Production Made Sritamin; I Dewa Putu Singarsa
Advances in Tropical Biodiversity and Environmental Sciences Vol 1 No 1 (2017): ATBES
Publisher : Institute for Research and Community Services Udayana University

Meloidogyne spp. Are among the polyphagous pest that has spread around the world and has been reported to attack cultivated plants with economic values. Various ways had been applied to control the Meloidogyne spp. nematodes but has not yet showed any effective results. We are currently using synthetic pesticides because of its fast response and ability to maintain plant productions. The improper application of synthetic nematicides would have a negative impact on the environment. The aim of this research is to know the effectiveness of betel leaf concentration (Piper betel L.) to curb the development of nematodes on Root-Knot Meloidogyne spp and the growth of tomato plants. This is a descriptive research, with the utilization of betel leaf extract (Piper betel L,) by concentration treatment of 0 %, 5 %, 10 %, 15 % and 20%, each of which are repeated five times. Complete Random Sampling (CRS) with the F test is used and continued with the Duncan test at a 5% rate. The result shows that the betel leaf extract of the 20% concentration was the most effective either on the growth of plants and to the root-knot nematodes. The suppression of growth in the ground was 80,06% and the lowest was recorded with the 5% concentration ( 2,32 %); the suppression of root-knot was 45,45 % while the lowest was with the 5% concentration (12,12 %); the suppression of nematodes population in the roots was 45,45% while the lowest was with the 5% concentration (39,76 %); and last was the suppression of egg mass which was 61,73 % while the lowest was shown in the 5% concentration (18,32 %). It can be concluded in this research that the most effective betel leaf extract concentration was at 20%.Keywords: Meloidogyne spp., Piper betel L., CRS

Isolasi dan Skrining Bakteri Selulolitik pada Feses Luwak I Putu Eko Arymanto Wira Kesuma; I Nyoman Wijaya; Made Sritamin
Nandur Vol 1 No 3 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Civet (Paradoxurus hermaphroditus) are carnivorous animals, but also often eat fruit and one of them is coffee cherries. The coffee cherries that are eaten are not completely digested, so the coffee beans come out with the civet feces. In the civet digestion there are various kinds of bacteria that help the digestive process, one of them is cellulolytic bacteria. It is possible that coffee beans in civet feces contain cellulolytic bacteria.This study aims to determine the presence of cellulolytic bacteria in civet feces. The results of bacterial isolation in civet feces obtained 6 isolates that had different morphological characters. Cellulolytic bacteria screening results showed that 2 out of 6 isolates were positive as cellulolytic bacteria, namely FL2 isolate with a cellulolytic index of 2.38 (high reaction) and FL3 isolate with a cellulolytic index of 1.24 (medium reaction). The isolates FL2 and FL3 had different morphological characters in colony shape, margins and color, but had similarities in elevation, gram color and cell shape.

Identifikasi Senyawa Fitokimia pada Daun Kersen (Muntingia calabura Linn.) dan Uji Toksisitasnya Terhadap Larva Udang Artemia salina Leach Ade Febrianto Umbu Romu Kolambani; Made Sritamin; I Nyoman Wijaya
Nandur Vol 1 No 4 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Singapore cherry (Muntingia calabura L.) is a plant that has shady trees, is often used as a shade and is easy to grow in various places. All parts of the cherry plant, including roots, bark, fruit, and leaves, contain secondary metabolites that can be used as traditional medicine. The purpose of this study was to determine the phytochemical compounds contained in cherry leaf extract and to determine the level of acute toxicity (LC50) using the Brine Shrimp Lethality Test (BSLT) method. Identification of phytochemical compounds was carried out by gas chromatography-mass spectrometry and acute toxicity test (LC50) was carried out using the BSLT method using the shrimp larvae test insect Artemia salina Leach. The acute toxicity test (LC50) consisted of 6 concentration treatments, namely 500 ppm, 250 ppm, 150 ppm, 100 ppm, 50 ppm, along with negative controls, each of which was repeated three times. The LC50 value is obtained from probit analysis in Microsoft Office Excel. There are 3 groups of bioactive compounds with the highest peak area in singapore cherry leaf extract, namely groups of flavonoids, phenols, and alkaloid. The result of acute toxicity test (LC50) of singapore cherry leaves extract was 84,029 ppm.

Studi Komparasi Jenis Pelarut Ekstraksi terhadap Kandungan Fitokimia Daun Tanaman Kubis Ungu Hengky Surya Bhuana; Made Sritamin; I Gede Putu Wirawan
Nandur Vol 1 No 4 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Red cabbage is a plant that is rich in antioxidant, one of its secondary metabolites, antosianin that gave the purple color on red cabbage has multiple health effects for human such as preventing cancer, tumor, and many more. The use of this secondary metabolites in plants nowadays is very popular, not only for its pharmacological effects, but also as an antibacterial, coloring agent, flavoring agent, natural pesticide, etc. Therefore, this study is needed to look for another potentially benefical compound in the plant, such as red cabbage, a plant that is more than just a salad. The aim of this study was to determine the effect of the types of extraction solvents on phytochemical compound of red cabbage plant leaves. The dried off plant macerated with two kinds of solvents, aquades, and ethanol. After about +3 days, both of the extract got evaporated with rotary evaporator. The yields of evaporating will be tested with GCMS analysis. The result showed that kind of extraction solvents do affect the yield from evaporating, and GC-MS analysis. Aquades showed to have a better capability in both of extraction, or on the analysis with GC-MS.

Isolasi dan Identifikasi Bakteri Selulolitik pada Buah Kopi Arabika (Coffea arabica L.) Nona Au Waling; Made Sritamin; I Nyoman Wijaya
Nandur Vol 1 No 3 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Cellulolytic bacteria are microorganisms that can produce cellulase enzymes. Cellulolytic bacteria contain cellulase enzymes that play a role in the cellulose degradation processes. The purpose of this study was to isolate cellulolytic bacteria that capable to degrading cellulose from arabica coffee cherries collected from Catur Village, Kintamani. The results of bacterial isolation at 10-6 and 10-8 dilutions obtained 10 bacterial colonies. From the results of bacterial purification, 4 different bacterial isolates had different morphological characters. The results of the isolation of the colonies showed differences in a round and irregular shape, had raised and convex surfaces had red, cream, white and yellow colors, had curved and smooth edges. Tests were carried out on four bacterial isolates to determine the clear zone index. Only the L isolate in the congo red test had a diameter of clear zone 2.5 mm and with score 3 based on cellulolytic index value L isolate categorized as strong degrading cellulose bacteria.

Co-Authors Abd. Rasyid Syamsuri Ade Febrianto Umbu Romu Kolambani ANAK AGUNG GEDE GARBA YOGANTARA Aulanni ‘am Aulanni ‘am BAYU ARYA BAGASKARA CATUR KOESUMA WARDHIANY DARWIN SILALAHI DELVIANA PANJAITAN DEWA AYU RATIH DIMINDA ELA SRI ERTINA BR GINTING EKA BHUWANA BONAULI BARITA Evy Prima Fransen Sinaga Gresia Eldina GUSTI PUTU DINTYA PUTRA Haryadi, Celvin Hengky Surya Bhuana I DEWA PUTU SINGARSA I G. N. Bagus I G. Ngurah Bagus I G.N. Bagus I Gede Ketut Susrama I GEDE PUTU WIRAWAN I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Suada I KETUT SUMIARTHA I M. Sudarma I Made Mega Adnyana I N. Wijaya I N. WIJAYA I NYOMAN TRYADI CAHYA NUGRAHA I NYOMAN WIJAYA I Putu Eko Arymanto Wira Kesuma I. G. N Bagus I. N. Wijaya IDA BAGUS MADE DWI JAYA KATARINA IKA NOVIANITA KETUT AYU YULIADHI Liliek Sulistyowati LUH PUTU SUGIARI M Sudarma M. Puspawati Made Adnyana MAGNA DWIPAYANA MUSLIM MUSLIM NI KADEK BUDARTINI NI KADEK DWI PASARI Ni Kadek Emi Sintha Dewi NI KADEK SINTYA DEWI Ni Kadek Sintya Dewi NI LUH MADE PRADNYAWATHI Ni Made Puspawati NI MADE PUTRI YUDANTARI Nona Au Waling NURUL KHOTIMAH PUTU ANA DIANTARI PUTU AYU MEITA YUDIA DEWI PUTU BAMBANG IKA WINJALISTA PUTU PADMA MAHESWARI Rindang Dwiyani RISMA IMROATUS SHOLIHAH Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sasadara, Maria Malida Vernandes Shara Yulita Harianja SHEILA SIMANJUNTAK SUNARTI TAMBUNAN TRIAN WANGSA PURWANTO TRISNA AGUNG PHABIOLA VLORA VERONICA SIPANGKAR WAYAN ADIARTAYASA Wijaya I N. Yundari, Yundari

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