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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Entomologi Indonesia Advances in Tropical Biodiversity and Environmental Sciences Berkala Penelitian Hayati Nandur
MADE SRITAMIN
Program Studi Agroekoteknologi Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Identifikasi Mikoriza Vesikular Arbuskular pada Rhizosfer Kopi Arabika (Coffea arabica L.) dan Kopi Robusta (Coffea robusta L.) dan Perbanyakannya dengan Media Zeolit PUTU AYU MEITA YUDIA DEWI; MADE SRITAMIN; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.2, April 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Vesicular Arbuscular Mycorrhizae Identification of Arabica Coffee(coffea arabica L.) and Robusta Coffee (Coffea robusta L.) Rhizosphere andIts Spore Multiplication in Zeolite Media.The high demand of coffee in the world led the farmers to improve coffee productivity by using inorganic fertilizers. Considering potential problems that may occured due to inorganic fertilizer use, aplication of biological fertilizer which one of them is containing Vesicular Arbuscular Mycorrhizae (VAM) could be expected to assist the growth of Arabica coffee and Robusta coffee plants in more naturally manner. This study aimed to determine the types mycorrhizal of rhizosphere coffee plants and determine the effectiveness of zeolite media and corn symbiont plant in propagation of VAM. The study began in April to August 2015. Spore isolation was done by conducting wet sieving method. Roots colonization percentages were calculated by root staining method and spores multiplication through trapping culture method. The results showed that VAM spores found in the rhizosphere arabica coffee plants are two genera VAM Acaulospora and Glomus, whereas in robusta coffee plant found three genera Acaulospora, Glomus, and Gigaspora. Mycorrhizal structures found in the rhizosphere of Arabica and Robusta coffee plants were arbuscular, vesicles, hyphae and inner spores. Corn symbionts plant and zeolite media were good combination for the propagation of VAM spores.
Uji Efektifitas Ekstrak Daun Sirih (Piper Betle L.),Kirinyuh (Chromoloena Odorata) dan Tembelekan (Lantana Camara L.) Terhadap Populasi Nematoda Puru Akar (Meloidogyne Spp.) dan Pertumbuhan Tanaman Cabai (Capsicum Annuum L) MAGNA DWIPAYANA; I NYOMAN WIJAYA; MADE SRITAMIN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Effectiveness Test of Extracts Of Betel Leaf (Piper Betle L.), Kirinyuh (Chromoloena Odorata) and Tembelekan (Lantana Camara L.) Against The Population of Root Knot Nematodes (Meloidogyne Spp.) and Plant Growth of Chili (Capsicum Annuum L.)Meloidogyne spp. was one of the parasitic nematode pests on crops of chili. Meloidogyne spp. was widespread in tropical regions and subtropik area.. Attacks of Meloidogyne spp. at the root can lower the production of the crops by 15 – 60 percent, even can reach 70 percent when the disease attacks vulnerable plants (Prihanto,1989). This research was carried out in a way controlling root knot nematodes on chili uses extract of 3 species of plants in a variety of concentrations with the aim to obtain a concentration from the plants that are very effective at suppressing the development of root knot nematodes and can suppress the population in the soil and as well to surpress the attacks on plant roots.The types of plants that will be used in this research is kirinyuh (Chromolaena odorata), tembelekan (Lantana camara) and betel leaf (Piper betle) in different concentrations to know their ability to suppress populations of root knot nematodes of chili. The leaves of the plant contains useful materials to suppress plant pests including root knot nematodes. From the three of leaf extract that have been used, the betel leaf extract effectively suppress the populations of nematodes on crops of chili for being able to put pressure against the population of the nematode in the soil or plant roots of chili. At the concentrations of 50 cc/polybag betel extracts are able to suppress the nematode population to 93% and on the concentration of 200cc/ polybag betel extracts was able to suppress populations of nematodes were 96.4%.
Aplikasi Teknik Polymerase Chain Reaction (PCR) Terhadap Variasi Gejala Penyakit Citrus Vein Phloem Degeneration (CVPD) pada Beberapa Jenis Daun Tanaman Jeruk GUSTI PUTU DINTYA PUTRA; WAYAN ADIARTAYASA; MADE SRITAMIN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.2, April 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Application Polymerase Chain Reaction (PCR) Technique to Variation Symptoms Disease Citrus Vein Phloem Degeneration (CVPD) on Several Types of Citrus Leaves CVPD disease is one of the major diseases in citrus plant. CVPD disease spread from plant to plant by insect vectors Diaphorina citri, and from one region to another region infected by seed. CVPD disease causes symptoms, the leaves become chlorotic with veins remain green. This study aims to determine the PCR method can detect CVPD of citrus from mild to severe symptoms. CVPD symptoms observed on several types of citrus leaves (Siam, Selayar, Besakih, Tejakula, Sweet, Purut, Lime, Lemo, and pomelo) showed chlorosis symptoms from mild to severe. Then the plants were symptomatic will be identified molecular in the Biotechnology Lab. After electrophoresis on almost all samples showed DNA bands with size 1160 bp. Therefore 1160 bp was had bacteri Liberobakter, the plants reacted positive with Liberobakter asiaticum Keyword: CVPD, Liberobacter asiaticum, PCR
Study of Some Weeds Extract to Control Root Knot Nematodes Meloidogyne spp. on Tomato Licopersicum esculentum Mill CATUR KOESUMA WARDHIANY; MADE SRITAMIN; KETUT AYU YULIADHI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.1, Januari2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Study of Some Weeds Extract to Control Root Knot Nematodes Meloidogyne spp. on Tomato Licopersicum esculentum Mill Root knot is the disease that caused by root knot nematodes Meloidogyne spp. Nematodes growth on the roots of plants that clog nutrients and water , blockage will cause impaired photosynthesis process of plants and plant nutrient deficiency symptoms are visible. This research using kirinyuh weed (Chromolaena odorata), kerasi weed (Lantana camara), babadotan weed (Ageratum conyzoides), alang- alang weed (Imperata cylindrica), and semanggi weed (Marsilea crenata) with a rate of 1:10 where the extract used is 250 cc of each extract weed liquid. The result of nematode population per 300 g of soil showed babadotan extract can suppress nematode populations Meloidogyne spp. only about 13 pcs/300 g soil, with the percentage of 97.4 % suppression, next is kerasi extract 19 pcs/300 g soil (96.2%), kirinyuh extract 19 pcs/300 g soil (96.2%), alang-alang extract 20 pcs/300 g soil (96%), and semanggi extract 37 pcs/300 g soil (92.6%). The result of nematode populations per 1 g of root, kerasi extract is the best suppress root knot nematode populations, there are only 37 pcs/ 1 g roots, with an emphasis percentage of 92.6%. next kirinyuh extracts 40 pcs / 1 g roots (92%), alang-alang extracts 54 pcs/ 1 g roots (89.2%), semanggi extract 60 pcs/ 1 g roots (88%), and the last is the babadotan extract 70 pcs/ 1 g roots (86%) .   Keywords: Ageratum conyzoides, Chromolaena odorata, Imperata cylindrica, Lantana camara, Licopersicum esculentum Mill., Marsilea crenata, Meloidogyne spp..
Keberadaan Mikroorganisme Tanah pada Areal Rehabilitasi Takino Soil Protection Sheet dan Kemampuan Menahan Erosi Permukaan di Kaldera Gunung Batur I NYOMAN TRYADI CAHYA NUGRAHA; MADE SRITAMIN; I GEDE PUTU WIRAWAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.4, Oktober 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Existence of Microorganism On Rehabilitation Area by Takino Soil Protection Sheet and Capability to Resist Soil Erosion in the Vulcanic Devastated Site in Mt. Batur The goal of this research was to know the existence of microorganism on rehabilitation area of Takino Soil Protection Sheet (TSPS) and capability to resist soil erosion in the vulcanic devastated site of Mt. Batur. This research used total plate count method to obtain mikroorganism colony, soil separating method to obtain mycorrhizal fungi spores, staining root method to find out percentage of mycorrhizal fungi spores on the roots and capability to resist erosion by measuring the soil trapped on the TSPS. The isolation results showed the number of bacteria colonies has been increase as much as 123%, while the number of fungi spores also increase as much as 100% in the area covered by TSPS. The results of trapping mycorrhizal fungi spores on the area covered by TSPS is 63/100g soil, in the area non covered by TSPS is 21/100g soil. The percentage of mychorrizal fungi infection in the plant roots on the area covered by TSPS is 27,5%. The result showed the average capability of TSPS to resist erosion is 5,26 cm over 3 years after implementation of TSPS.
Pengaruh Variasi Waktu Inkubasi dengan Penambahan Paya Meat Tenderizer pada Proses Dekafeinasi Kopi Arabika (Coffea arabica L.) DEWA AYU RATIH; MADE SRITAMIN; I GEDE PUTU WIRAWAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.1, Januari 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Effect of Incubation Time Variation with The Addition of Paya Meat Tenderizer in the Arabica Coffee Decaffeination Process (Coffea arabica L.) High levels of caffeine lead to insomnia and increased blood pressure. Decaffeination is a solution to reduce the caffeine with enzymatic method with papain coarse extract PAYA Meat Tenderizer contains proteolytic activity of 1,0593 units/g. The study aims to find out the effect of arabica coffee incubation time with the addition of the enzyme papain PAYA Meat Tenderizer. The study looked at protein levels (Kjeldahl), caffeine levels (UV-Vis spectrophotometry) and organoleptic tests. The design used is Complete Random Design (RAL) 4 incubation time treatment i.e. B0 (0 hours); B1 (12 hours); B2 (24 hours) and B3 (36 hours). The results showed a very real influential treatment of all observed variables. B2 (24-hour incubation) lowers caffeine levels by 0,006%. A 0,12% decrease in protein levels occurred in 36-hour incubation. The highest results on organoleptic tests of aroma, viscosity and bitter taste parameters were obtained control samples (B0) and 36-hour (B3).
Uji Efektifitas Berbagai Konsentrasi Ekstrak Daun Tanaman Terhadap Penekanan Populasi Nematoda Puru Akar (Meloidogyne spp.) dalam Tanah, Akar, dan Produksi Tanaman Tomat (Lycopersicum esculentum Mill.) NI MADE PUTRI YUDANTARI; MADE SRITAMIN; I DEWA PUTU SINGARSA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.3, Juli 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Test the Effectiveness of Various Concentrations of Plant Leaf Extracts Against Root Knot Nematode Suppression (Meloidogyne spp.) In Soil, Roots, and Production Tomato Plants (Lycopersicum esculentum Mill.). Root knot disease is a disease caused by root knot nematodes Meloidogyne spp. Nematodes grown on the roots of plants by inhibiting nutrients that interfere with the process of photosynthesis and stunted plants showing symptoms, while wilting, and permanent wilting on the plant at ground level. While the plants in the basement there is a knot at the root. In this study to control root knot Meloidogyne spp. using sirih (Piper betle Linn.), kirinyuh (Chomolaena odorata Linn.), and tembelekan (Lantana camara Linn.) with a concentration of 100 cc/polybag, 200 cc/polybag, and 300 cc/ polybag of each extract solution leaves the test.The results of the calculation of the population of nematodes per 300 g soil showed sirih extract suppressed the population of root knot nematodes Meloidogyne spp. the good, 23 pcs/??300 g soil, with an emphasis percentage of 95.4%, next is to extract kirinyuh 26 psc/300 g soil (94.7%), and extract tembelekan 27 psc/300 g soil (94.4 %). In the calculation of the population of root knot nematodes Meloidogyne spp. per 1 g of roots, extract of piper betle was the most good in suppressing root knot nematode populations, there are only 22 pcs/1 g roots, with a percentage of 95.5%, followed by extracts of kirinyuh 24 pcs/1 g roots (95.1%) , and extract of tembelekan 28 psc/1 g roots (94.3%).   Keywords: Chromolaena odorata Linn., Lantana camara Linn., Lycpersicum esculentum Mill., Piper betle Linn..
Struktur Histopatologi Tangkai Daun Jeruk Siam (Citrus nobilis L. var. microcarpa) Terinfeksi Citrus Vein Phloem Degeneration (CVPD) pada Tingkat Serangan Ringan dan Berat TRIAN WANGSA PURWANTO; MADE SRITAMIN; NI LUH MADE PRADNYAWATHI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.1, Januari 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Histophatology Structure of Siam Citrus Petiole (Citrus nobilis L. var. microcarpa) Infected by Citrus Vein Phloem Degeneration (CVPD) at Mild and Severe Attack Rates The main cause of decreasing the production of Citrus nobilis L. var. microcarpa in Indonesia is the presence of CVPD disease (Citrus Vein PhloemDegeneration). Citrus nobilis L. var. microcarpa infected by CVPD showing anatomic and morphological changes. The main objective of this study was todetermine anatomic changes in petiole tissue of Citrus nobilis L. var. microcarpa infected by CVPD at the level of mild and severe attacks. The research wasconducted in Citrus field, Kintamani, Bangli-Bali and Biomolecular and Genetic Resources Laboratory, Faculty of Agriculture, Udayana University in August September 2018. The result for visual observation showed that the presentasion of CVPD symptoms in the field was 46% and attack intensity was 39.6%. Samples were identified using PCR metod with O11 and O12c markers. DNA visualization amplified on 1% agarose showed appearing of DNA band for samples with mild and severe attack rates. Samples gave positive reaction to Liberibacter asiaticus. It showed that the samples were positive infected by CVPD disease. Preparations of petioles were made to observe the histological structure microscopically. The result showed that there were changes on histological structure of petioles infected CVPD. Petioles infected CVPD at mild symptom and severe symptom showing cell wall thickening on epidermis, shape changes on cortex and there were an accumulation of starch, lysis and necrosis showing on floem with severe attack rate, and there were stretching between xylem cells.
Induksi Tunas Tanaman Rasberi Hitam (Rubus occidentalis L.) Melalui Direct Organogenesis Secara In Vitro LUH PUTU SUGIARI; MADE SRITAMIN; RINDANG DWIYANI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.4, Oktober 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Induction of Black Raspberry Buds (Rubus occidentalis L.) Through Direct Organogenesis In Vitro. Supervised Black Raspberry plants are sources of various bioactive compounds such as phenolic compounds or phenolic acids, flavonoids, anthocyanins, flavonols, and tannins that are used as medicine. Black Raspberry also have a wide market share, the price reaches Rp 200,000 per kg. Propagation of black raspberry in a conventional way requires a long time. This study aims to obtain black raspberry seedlings in a relatively short time and large quantities through direct organogenesis in vitro. This research was divided into two, namely research on the sterilization method and the effect of gowth regulators with six treatments. The data were analyzed descriptively. The results of this study indicated that sterilization using tween 20 and vitamin C could reduce the occurrence of contamination and browning in explants respectively by 33.3% and 100%. The used of MS media + 2 ppm BAP + 150 ml coconut water + 0.01 ppm NAA (K4) treatment showed a response of gowth of shoots and leaves on the plantlet of 100%.
Aplikasi Ekstrak Bahan Nabati Berbagai Tanaman terhadap Perkembangan Populasi dan Reproduksi Nematoda Puru Akar Meloidogyne spp. pada Tanaman Tomat (Lycopersicum esculentum Mill.) PUTU ANA DIANTARI; MADE SRITAMIN; I GUSTI NGURAH BAGUS
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Application of plant extracts to control the population and reproduction of Meloidogyne spp. on tomato (Lycopersicum esculentum Mill.) Root knot nematodes Meloidogyne spp. was one of the pests that can be a limiting in crop cultivation. Efforts to control nematodes in general performed chemically by using synthetic nematicides. Use this Nematisida can negatively impact the environment, especially when the use of nematicides is too excessive. To try to avoid it in a way that is friendly to the environment control using plant-based materials.The purpose of this study was to determine the plant extracts were able to suppress the population of Meloidogyne spp. and determine the most effective plant extracts suppress the population of Meloidogyne spp. the results showed that the nematode population in the soil of 300 g show the lowest was found in the extract treatment plant Piper betle L. is 25 head, with a percentage of 95%, followed by treatment of Carica papaya tail 28.75 (94.25%), Nicotiana tabacum tail 32.25 (93.55%), Allium sativum 49.5 tail (90.1%), Allium sativum 56.5 tail (88.7%), Riccinus tail communis 63.25 (87.35%), Datura stramonium L. 65 individuals (87%), Morinda citrifolia tail 68.28 (86.34%). To the amount of 1 g of root galls in the most tangible effect of treatment indicated by the plant extract of Piper betle L. with the average number of root knot per 1 g of root pieces with a percentage of 96.5 to 17.5%, followed by treatment of the plant Carica papaya extracts 20, 5 pieces (95.9%), Nicotiana tabacum L. 22.5 units (95.5%), Allium sativum fruit 24.5 (95.1%), Capsicum frutescens L. fruit 26.75 (94.65%), Riccinus communis fruit 28.5  (94.3%), Datura stramonium L. 30.5 units (93.9%), Morinda citrifolia L. fruit 32.25 (93.55%). root knot nematode populations in a 1 g root is: Piper betle L. plant extract with an average of 23.75 tails nematode populations with emphasis percentage (95.25%), followed by treatment of Carica papaya 26.0 tail (94.8% ), Nicotiana tabacum L. tail 28.75 (94.25%), Allium sativum tail 30.75 (93.85%), Capsicum frutescens L. 34.0 tail (93.2%), 36.75 Riccinus communis tail (92.65%), Datura stramonium L. tail 42.75 (91.45%), Morinda citrifolia L. 44.0 tail (91.2%). number of egg masses in the 1 g roots of plants treated with each treatment with the control test plant extracts showed Piper betle L. plant extracts resulted in suppression of egg masses is simply the most good 4.0 percentage points to 99.2% suppression, followed by Carica papaya 6.5 points (98.7%), Nicotiana tabacum L. 9.0 points (98.2%), Allium sativum 10.5 points (97.9%), Capsicum frutescens L. 11.75 grains (97, 65%), Riccinus communis 13.25 points (97.35%), Datura stramonium L. 15.0 points (97%), Morinda citrifolia L. 17.25grains(96.5%). Keywords:Caricapapaya,Nicotianatabacum L., Piper betle L.Tomato plants and Meloidogyne spp.
Co-Authors Abd. Rasyid Syamsuri Ade Febrianto Umbu Romu Kolambani ANAK AGUNG GEDE GARBA YOGANTARA Aulanni ‘am Aulanni ‘am BAYU ARYA BAGASKARA CATUR KOESUMA WARDHIANY DARWIN SILALAHI DELVIANA PANJAITAN DEWA AYU RATIH DIMINDA ELA SRI ERTINA BR GINTING EKA BHUWANA BONAULI BARITA Evy Prima Fransen Sinaga Gresia Eldina GUSTI PUTU DINTYA PUTRA Haryadi, Celvin Hengky Surya Bhuana I DEWA PUTU SINGARSA I G. N. Bagus I G. Ngurah Bagus I G.N. Bagus I Gede Ketut Susrama I GEDE PUTU WIRAWAN I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Suada I KETUT SUMIARTHA I M. Sudarma I Made Mega Adnyana I N. Wijaya I N. WIJAYA I NYOMAN TRYADI CAHYA NUGRAHA I NYOMAN WIJAYA I Putu Eko Arymanto Wira Kesuma I. G. N Bagus I. N. Wijaya IDA BAGUS MADE DWI JAYA KATARINA IKA NOVIANITA KETUT AYU YULIADHI Liliek Sulistyowati LUH PUTU SUGIARI M Sudarma M. Puspawati Made Adnyana MAGNA DWIPAYANA MUSLIM MUSLIM NI KADEK BUDARTINI NI KADEK DWI PASARI Ni Kadek Emi Sintha Dewi Ni Kadek Sintya Dewi NI KADEK SINTYA DEWI NI LUH MADE PRADNYAWATHI Ni Made Puspawati NI MADE PUTRI YUDANTARI Nona Au Waling NURUL KHOTIMAH PUTU ANA DIANTARI PUTU AYU MEITA YUDIA DEWI PUTU BAMBANG IKA WINJALISTA PUTU PADMA MAHESWARI Rindang Dwiyani RISMA IMROATUS SHOLIHAH Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sasadara, Maria Malida Vernandes Shara Yulita Harianja SHEILA SIMANJUNTAK SUNARTI TAMBUNAN TRIAN WANGSA PURWANTO TRISNA AGUNG PHABIOLA VLORA VERONICA SIPANGKAR WAYAN ADIARTAYASA Wijaya I N. Yundari, Yundari