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All Journal BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Pasir Laut Bioma : Berkala Ilmiah Biologi Jurnal Akademika Biologi Management of Aquatic Resources Journal (Maquares) Journal of Marine Research AL KAUNIYAH Jurnal Kelautan Tropis Indonesian Journal of Chemical Science JURNAL ENGGANO JFMR (Journal of Fisheries and Marine Research) Jurnal Laut Khatulistiwa Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Journal of Aquaculture and Fish Health International Journal of Islamic Education, Research and Multiculturalism (IJIERM) Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Aninditia Sabdaningsih
Departemen Sumberdaya Akuatik, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Engineering Environmental Science Materials Science & Nanotechnology Physics Other

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Journal : AL KAUNIYAH

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Identification and Phylogenetic Analysis of Blue Swimming Crab (Portunus sp.) in The Northern Waters of Jepara Used DNA Barcoding Lazuardhi, Risqi; Sabdaningsih, Aninditia; Ayuningrum, Diah; Saputra, Suradi Wijaya; Hartoko, Agus; Haeruddin, Haeruddin
Al-Kauniyah: Jurnal Biologi Vol 18, No 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.38705

Abstract

The Awur Bay and Kartini Beach in Jepara, Central Java, are fishing areas with blue swimming crab as a main catch. This study aims to examine the species of blue swimming crabs captured in the Northern Waters of Jepara from different locations, analyze the molecular characteristics based on gender differences, obtain GenBank accession numbers as genetic conservation, and construct their phylogenetic tree. The research used a quantitative descriptive method. Samples were collected using purposive sampling from fishermen's catches and selected based on the difference in crab claws and carapace color, with a total of 4 samples. The samples were extracted using a DNA extraction kit and tested using Polymerase Chain Reaction (PCR) to visualize mitochondrial DNA (COI gene) and processed using MEGA 11. BLAST results showed that the similarity of the samples to Portunus pelagicus ranged from 97.02% to 99.42%. The different colors of claws and carapace did not affect the DNA sequence composition, hence indicating sexual dimorphisms. The DNA sequences from this study were deposited to GenBank with accession number LC836055.1-LC836058.1. Based on the phylogenetic tree construction, the clade of all samples shows a close relationship with the species Portunus pelagicus.
Identification and Phylogenetic Analysis of Blue Swimming Crab (Portunus sp.) in The Northern Waters of Jepara Used DNA Barcoding Lazuardhi, Risqi; Sabdaningsih, Aninditia; Ayuningrum, Diah; Saputra, Suradi Wijaya; Hartoko, Agus; Haeruddin, Haeruddin
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.38705

Abstract

The Awur Bay and Kartini Beach in Jepara, Central Java, are fishing areas with blue swimming crab as a main catch. This study aims to examine the species of blue swimming crabs captured in the Northern Waters of Jepara from different locations, analyze the molecular characteristics based on gender differences, obtain GenBank accession numbers as genetic conservation, and construct their phylogenetic tree. The research used a quantitative descriptive method. Samples were collected using purposive sampling from fishermen's catches and selected based on the difference in crab claws and carapace color, with a total of 4 samples. The samples were extracted using a DNA extraction kit and tested using Polymerase Chain Reaction (PCR) to visualize mitochondrial DNA (COI gene) and processed using MEGA 11. BLAST results showed that the similarity of the samples to Portunus pelagicus ranged from 97.02% to 99.42%. The different colors of claws and carapace did not affect the DNA sequence composition, hence indicating sexual dimorphisms. The DNA sequences from this study were deposited to GenBank with accession number LC836055.1-LC836058.1. Based on the phylogenetic tree construction, the clade of all samples shows a close relationship with the species Portunus pelagicus.
Co-Authors Abdul Qadir Jailani, Abdul Qadir Achmad Mufidh Karomatullah Adriyani, Alvita Afwa, Rainuy Saninzita Agus Hartoko Agus Sabdono Agus Trianto Anakotta, Anthoinette Rosaline Fransisca Anhar Solichin Anhar Solichin Anto Budiharjo Arif Rahman Arina Tri Lunggani Aristi Dian Purnama Fitri Asriyana, . Bahry, Muhammad Syaifudien Baroqi, A. Riza Churun Ain Delianis Pringgenies Delianis Pringgenies DIAH AYUNINGRUM Diah Ayuningrum, Diah Dianti Eka Yurnaningsih Diva Triza Novitasari Endang Kusdiyantini Fadhila, Zalfa Langgeng Febrianto, Sigit Fitria Sari Fitria Sari Fitriadi, Ren Haeruddin Haeruddin Haeruddin Haeruddin Huda, Anisa Arifatul Karohmatullah, Achmad Mufidh Kharisma Ayu Zeina Halisah Lazuardhi, Risqi M. Rehatta, Beatrix Mada Triandala Sibero Max Rudolf Muskananfola Millatia, Zulfa Muhammad Zainuddin Muhammad Zainuddin Muhammad Zainuddin Muhammad Zainuddin Nailirohmah, Ulya Melly Natalie, Gyorgiana Gilda Niniek Widyorini Niniek Widyorini Novitasari, Diva Triza Nur Handayani Ocky Karna Radjasa Ocky Karna Radjasa Ocky Karna Radjasa Oktavianto Eko Jati Pujiono Wahyu Purnomo Pujiono Wahyu Purnomo Putra, Muhammad Ardinan Dwi Rahmadhati, Kharisma Yulia Rahmawati, Silviana Dian Ratna Diyah Palupi Rizaldi, Rahmat Rosari, Arinda Sakina, Kurnia Sarjito - Siti Rudiyanti Slamet Budi Prayitno slamet budi prayitno Soedibya, Petrus Hary Tjahja Subagiyo Subagiyo Subagiyo Subagiyo Subhan Subhan Suradi Wijaya Saputra Suradi Wijaya Saputra Suradi wijaya Saputra Suradi Wijaya Saputra Suryanti Tahrin, Maria Taufani, Wiwiet Teguh Timur, Putra Satria Vivi Endar Herawati Vivi Endar Herawati Widjaya Saputra, Suradi Yurnaningsih, Dianti Eka