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All Journal Jurnal Akuakultur Rawa Indonesia Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Perikanan dan Kelautan Jurnal Kelautan Tropis JFMR (Journal of Fisheries and Marine Research) BIOEDUSCIENCE Jurnal Penamas Adi Buana Journal of Aquaculture and Fish Health Kontribusia : Research Dissemination for Community Development Grouper: Jurnal Ilmiah Perikanan Jurnal Perikanan Pantura Journal of Fisheries & Marine
Sari, Putri Desi Wulan
Departement Of Aquaculture, Faculty Of Fisheries And Marine, Universitas Airlangga, Jl. Mulyorejo, Mulyorejo, Surabaya 60115, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Social Sciences Veterinary Other

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Journal : Journal of Fisheries

 1 
Variasi Morfologi Holdfast Lernaea yang Menyerang Ikan Gurami di Jawa Timur [Morphological Variation Of Lernaea Holdfast Infest Gouramy In East Java] Kismiyati Kismiyati; Putri Desi Wulan Sari
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11390

Abstract

Abstract Lernaeosis is one of the parasitic diseases in freshwater fish caused by Lernaea cyprinacea. These parasites infest many freshwater fish. Identification of parasites carried by observing the morphology of holdfast as an attachment organ. This study aims to make observations on the variation of morphology of Lernaea holdfast's in East Java and the identification of Lernaea infest gouramy in East Java.
Pengelolaan Limbah pada Pabrik Pengolahan Ikan di PT. Kelola Mina Laut Gresik [Waste Treatment at Fish Processing Company in Kelola Mina Laut Incorporated Gresik East Java Province] Putri Desi Wulansari
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11635

Abstract

AbstractIndustrial waste comes from the end of product process as a solid or liquid waste. Avoiding the negative effects of industrial waste, each industry must complete their company with wastewater treatment plant. This Field Job Practice was done at Kelola Mina Laut, Inc., located in Randu Agung Village, Kebomas District, Gresik th thResidence, East Java Province on August 4 – September 13 as a mean to observe, learn and to know Kelola Mina Laut, Inc. wastewater treatment method. Work method which used in Field Job Practice was descriptive method with data collection technique, active participation, interview and literature study. The wastewater treatment plant of Kelola Mina Laut, Inc. consists of one collector batch unit, two aeration batch units, one sedimentation batch unit, and one contol batch unit. Before loosing the effluent to the water stream, wastewater passes through the control batch to know about the wastewater quality. After passing through the treatments, wastewater that loosing upon the environment will have a good quality and appropriate with Environment Quality Standard. 
Perubahan Patologi Kulit Ikan Gurami (Osphronemu gouramy) Akibat Infestasi Lernaea cyprinacea Pada Derajat Infestasi Yang Berbeda [The Pathology Alteration Of Gouramy (Osphronemus gouramy) Integument Infested By Lernaea cyprinacea At Different Degress Of Infestation] Gunanti Mahasri; Putri Desi Wulan Sari; Setiawan Koesdarto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11640

Abstract

Abstract The development of fish consumption in Indonesia was promising increasingly and growing very rapidly. One of the priority commodities in the development of aquaculture subsector was the gourami (Osphronemus gouramy). Many factors must be faced in the cultivation of gouramy, including the problem of fish diseases caused by Lernaea cyprinacea. The aim of this research is to find out the description of anatomic pathology and histopathology of gouramy which infested by L. cyprinacea at different degrees of infestation. This research uses descriptive method. The research showed that the gouramy integument which infested by L. cyprinacea at low, moderate and heavy infestation cause moderate until heavy defect with 2.48; 2.42 and 2.32 in reaverage scoring. Hence, accordance with pathological anatomy, L. cyprinacea infestation caused necrotic nodules, lesion which covered by exudate and haemorrhage. While the histopathology of the integument changes found were inflammatory cell infiltration, congestion and haemorrhage in the low, moderate and heavy infestation.
Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches Rozi; Tyasningsih, Wiwiek; Rahmahani, Jola; Aksono, Eduardus Bimo; Yunus , Muchammad; Al-Arif, Mohammad Anam; Kuncorojati, Suryo; Kusdarwati, Rahayu; Sari, Putri Desi Wulan; Amal, Mohammad Noor Azmai; Salleh, Annas; Khanand, Nadeem; Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.62035

Abstract

Graphical Abstract Highlight Research The study aims to develop a multi-epitope vaccine (MEV) against A. hydrophila by targeting the aerolysin toxin, a key virulence factor responsible for infections in fish and humans. Computational methods identified and optimized B-cell and T-cell epitopes, focusing on their ability to trigger immune responses without causing toxicity or allergenicity. In silico simulations demonstrated that the MEV has a strong binding affinity to immune receptors like TLR-4, MHC-I, and MHC-II, indicating its potential to induce robust cellular and humoral immunity. Structural analysis of the MEV showed a stable 3D conformation, with most residues in favorable regions, ensuring stability during immune activation. The MEV could enhance disease control in aquaculture and reduce human infection risks, offering a promising solution to address antibiotic resistance and the absence of effective vaccines. Abstract Aeromonas hydrophila, gram-negative, is a major pathogen responsible for various diseases in mammals, reptiles, amphibia, and vertebrates, including fish and humans. Targeting the specific toxin aerolysin in A. hydrophila is crucial to address antibiotic resistance and the lack of adequate and protective vaccines against this intracellular pathogen. This study aimed to identify a multi-epitope vaccination (MEV) candidate targeting A. hydrophila aerolysin toxin to combat the disease effectively. Standard biochemical characterization methods and sequencing of the 16S rRNA, rpoB, and aerA genes identified the isolate AHSA1 as A. hydrophila. Subsequently, we identified B and T cell epitopes on the aerolysin protein and separately predicted MHC-I and MHC-II epitopes. The epitopes are then evaluated for toxicity, antigenicity, allergenicity, and solubility. The vaccine design integrated multi-epitope-based constructs, utilizing specialized linkers (GPGPG) and EAAAK linkers to connect epitope peptides with adjuvants in the cholera toxin B component, thereby enhancing immunogenicity. Ramachandran plots showed that 85.25% of the residues were located in the most favorable regions, which was followed by the generously allowed zone (1.30%), the additional allowed regions (10.80%), and the forbidden regions (2.65%), thus confirming the feasibility of the modeled vaccine design. Based on docking simulations, MEV had the highest binding and interaction energies with TLR-4, TLR-9, MHC-I, and MHC-II (-1081.4, -723.2, 866.2, -9043.3 kcal/mol). Based on computational modelling, we expect the Aerolysin MEV candidate design to activate diverse immune mechanisms, stimulate robust responses against A. hydrophila, and maintain safety. The significant solubility, absence of toxicity or allergic response, and minimal side effects in animal testing all contribute to the potential clinical utility of this vaccine candidate.  
Co-Authors Akbar Maulana Al Ishaqi Akhmad Taufiq Mukti Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Albet Surya Kembara Amal, Mohammad Noor Azmai Arida Vitasari Daruti Dinda Nindarwi Dewi, Nina Nurmalia Dwi Yuli Pujiastuti Endang Puji Astuti Fahdi Putra Utama Fauzan, Agung Luthfi Ferry Dwi Firmansyah Liananda Gunanti Mahasri Hari Suprapto Indah Hidayati Imani Khanand, Nadeem Kismiyati , Koesnoto Koesnoto Kuncorojati, Suryo Lalu Aldy Kurnia Aji Lia Oktavia Ika Putri Luthfiana Aprilianita Sari Mada Dewantara Mirni Lamid Muhamad Amin Muhammad Ghufron Nafis Putra Laksana Cholil Puguh Yugo Wijanarko Qurrota A'yun Rahayu Kusdarwati Rahmahani, Jola Regita Ardyanti Robi M.A Yahya Rozi Salleh, Annas Salman Aldo Alfaresi Setiawan Koesdarto Siti Hamidah Sri Subekti Suwarno Tjahjaningsih, Wahju Tyasningsih, Wiwiek Veryl Hasan Yarin Dwi Monica Yudin Ardha Pradipta Yunus , Muchammad