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All Journal VALENSI Jurnal Kimia (Journal of Chemistry) Jurnal MIPA CHEMISTRY PROGRESS agriTECH Molekul: Jurnal Ilmiah Kimia The Studies of Social Sciences Makara Journal of Science Molekul: Jurnal Ilmiah Kimia
Dewa Gede Katja
Jurusan Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Sam Ratulangi, Kampus Unsrat, Bahu Manado 95115
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Materials Science & Nanotechnology Mathematics Physics Social Sciences

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Aktivitas Antibakteri Ekstrak dan Fraksi Kulit Batang Chisocheton sp. (C.DC) Harms terhadap Staphylococcus aureus dan Escherichia coli Sujana, Kinaya Vizria; Katja, Dewa G.; Koleangan, Harry S. J.
CHEMISTRY PROGRESS Vol. 17 No. 1 (2024)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.17.1.2024.54700

Abstract

ABSTRAK Tumbuhan Chisocheton merupakan salah satu tumbuhan yang dapat dimanfaatkan untuk mengobati maupun sebagai sumber obat baru. Tumbuhan ini mengandung berbagai senyawa metabolit sekunder seperti fenolik, flavonoid, triterpenoid, dan tanin. Tujuan dari penelitian adalah untuk mengetahui kandungan total fenolik, flavonoid, dan tanin dari ekstrak dan fraksi kulit batang tumbuhan Chisocheton sp. (C.DC) Harms. Serta untuk mengetahui aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Ekstrak metanol kulit batang Chisocheton diperoleh dari proses ekstraksi maserasi 200 gr serbuk kulit batang Chisocheton dengan menggunakan pelarut metanol dan menghasilkan rendemen sebesar 11,670%. Sebanyak 5 gr ekstrak metanol kemudian dipartisi menggunakan pelarut n-heksana, etil asetat dan air sehingga diperoleh fraksi n-heksana, etil asetat dan air. Uji kandungan total fenolik, flavonoid dan tanin kulit batang Chisocheton ditentukan menggunakan metode spektrofotometer Uv-Vis. Aktivitas antibakteri diuji menggunakan metode difusi sumuran dengan membuat variasi konsentrasi (5%, 10%, 15%, dan 20%). Berdasarkan pada hasil penelitian diperoleh bahwa ekstrak metanol memiliki nilai kandungan total fenolik, flavonoid dan tanin tertinggi. Hasil uji antibakteri pada bebagai variasi konsentrasi tertinggi terdapat pada ekstrak metanol dengan besar zona hambat pada konsentrasi 20% sebesar 10,17 mm terhadap S.aureus dan 8,15 mm terhadap E.coli. Kata Kunci: Chisocheton sp., kulit batang, antibakteri   ABSTRACT The Chisocheton plant is one of the plants that can be used to treat and as a source of new drugs. This plant contains various secondary metabolite compounds such as phenolics, flavonoids, triterpenoids, and tannins. The purpose of the study was to determine the total phenolic, flavonoid, and tannin content of extracts and fractions of Chisocheton sp. (C.DC) Harms stem bark. And to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria. Methanol extract of Chisocheton stem bark was obtained from the maceration extraction process of 200 g of Chisocheton stem bark powder using methanol solvent and produced a yield of 11.670%. A total of 5 g of methanol extract was then partitioned using n-hexane, ethyl acetate and water solvents to obtain n-hexane, ethyl acetate and water fractions.  The total phenolic, flavonoid and tannin contents of Chisocheton stem bark were determined using the Uv-Vis spectrophotometer method. Antibacterial activity was tested using the pitting diffusion method by making concentration variations (5%, 10%, 15%, and 20%). Based on the results, it was found that the methanol extract had the highest total phenolic, flavonoid and tannin content values. The antibacterial test results at various concentrations were highest in the methanol extract with a large inhibition zone at a concentration of  20% of 10.17 mm against S.aureus and 8.15 mm against E.coli. Keywords:. Chisocheton sp., stem bark, antibacterial
Aktivitas Penghambatan Enzim ꭤ-Amilase oleh Ekstrak Etanol Hasil Soxhletasi dan Refluks Daun Manggis (Garcinia mangostana L.) Pettanaga, Putri Firania; Katja, Dewa Gede; Koleangan, Harry Steven Julius
CHEMISTRY PROGRESS Vol. 17 No. 2 (2024)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.17.2.2024.56254

Abstract

ABSTRAK Penelitian ini bertujuan untuk menentukan kandungan senyawa fenolik, flavonoid, dan tanin terkondensasi dalam ekstrak etanol yang diperoleh melalui soxhletasi dan refluks daun manggis dan untuk menentukan efektivitasnya terhadap penghambatan enzim ꭤ-amilase. Penelitian dikerjakan melalui tahapan-tahapan preparasi, ekstraksi, penentuan kandungan total fenolik, flavonoid, dan tanin terkondensasi, serta penentuan aktivitas penghambatan enzim ꭤ-amilase. Rendemen tertinggi diperoleh pada ekstrak etanol hasil refluks selama 4 jam (R4) (19,88%) dan terendah diperoleh pada ekstrak etanol hasil soxhletasi selamas 2 jam (S2) (7,97%). Kandungan total fenolik dan flavonoid tertinggi ditemukan pada ekstrak etanol hasil soxhletasi selama 8 jam (S8) (226,73 µg/mL dan 266,76 µg/mL). Kandungan tanin terkondensasi tertinggi diperoleh pada ekstrak etanol hasil soxhletasi selama 4 jam (S4) (24,36 µg/mL). Aktivitas penghambatan enzim ꭤ-amilase tertinggi ditunjukkan oleh ekstrak etanol hasil soxhletasi selama 4 jam (S4) 750 µg/mL (92,95%). Dapat disimpulkan bahwa ekstrak etanol daun manggis yang diperoleh melali proses soxhletasi lebih efektif dalam menghambat enzim ꭤ-amilase dibandingkan dengan yang diperoleh melalui proses refluks.   ABSTRACT This research was aimed to determine the content of phenolic compounds, flavonoids, and condensed tannins in ethanol extracts obtained through soxhletation and reflux of mangosteen leaves, and to determine their effectiveness in inhibiting the α-amylase enzyme. The research was conducted through the stages of preparation, extraction, determination of total phenolic, flavonoid, and condensed tannin content, as well as determination of α-amylase enzyme inhibition activity. The highest yield was obtained from the ethanol extract produced by reflux for 4 hours (R4) (19.88%) and the lowest was obtained from the ethanol extract produced by Soxhletation for 2 hours (S2) (7.97%). The highest total phenolic and flavonoid content was found in the ethanol extract produced by Soxhletation for 8 hours (S8) (226.73 µg/mL and 266.76 µg/mL). The highest condensed tannin content was obtained in the ethanol extract produced by Soxhletation for 4 hours (S4) (24.36 µg/mL). The highest α-amylase enzyme inhibition activity was shown by the ethanol extract produced by Soxhletation for 4 hours (S4) 750 µg/mL (92.95%). It can be concluded that the ethanol extract of mangosteen leaves obtained through the Soxhletation process is more effective in inhibiting the α-amylase enzyme compared to that obtained through the reflux process.
Pelatihan Pembuatan Obat Luka dari Limbah Daun Pala Bagi Ibu-ibu PKK Desa Pales, Kecamatan Likupang Barat, Kabupaten Minahasa Utara Katja, Dewa Gede; Koleangan, Harry Steven Julius; Pollo, Hard Napoleon; Sinaga, Jonathan Cavin Ezra
The Studies of Social Sciences Vol. 6 No. 2 (2024): The Studies of Social Sciences
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35801/tsss.v6i2.58432

Abstract

Kegiatan Program Kemitraan Masyarakat (PKM) telah dilaksanakan dengan judul Pelatihan Pembuatan Obat Luka Bagi Ibu-ibu PKK Desa Palaes, Kecamatan Likupang Barat, Kabupaten Minahasa Utara. Kegiatan ini diawali dengan produksi minyak atsiri dari limbah daun pala, serta dilanjutkan dengan perumusan kegiatan. Ibu-ibu PKK desa Palaes memiliki potensi untuk menjadi kader pembawa informasi kepada masyarakat sekitar tentang manfaat limbah daun pala yang dapat dijadikan plester luka. Kegiatan ini bertujuan untuk memberikan pengetahuan dan ketrampilan bagi masyrakat dalam memanfaatkan limbah daun pala. Metode yang digunakan adalah pelatihan tentang pembuatan plester obat dari limbah daun pala serta evaluasi kepada peserta. Dari kegiatan ini, Ibu-ibu PKK telah mampu memahami dan mengetahui cara produksi minyak atrisi dari limbah daun pala serta dapat membuat plester luka.
Steroids Produced by Endophytic Fungi (Fusarium phaseoli) Isolated from Chisocheton macrophyllus and their Antibacterial Activity against Escherichia coli and Staphylococcus aureuss Katja, Dewa Gede; Sari, Aprilia Permata; Sinaga, Siska Elisahbet; Nurlelasari, Nurlelasari; Farabi, Kindi; Sofian, Ferry Ferdiansyah; Fajriah, Sofa; Naini, Al Arofatus; Supratman, Unang
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.12727

Abstract

ABSTRACT. Steroids are secondary metabolic derivatives of terpenes containing the tetracyclic ring system known to exhibit fascinating pharmacological activity. Steroids are distributed in various genera of endophytic fungi including Fusarium genus which lives inside a higher tree such as Chisocheton macrophyllus. The purpose of this research is to identify and characterize the chemical structure of steroids generated by F. phaseoli, an endophytic fungus obtained from C. macrophyllus roots, as well as to assess their antibacterial activity against Escherichia coli and Staphylococcus aureus. The brown rice medium was fermented with F. phaseoli for six weeks before extraction with ethyl acetate. The extracts yielded four compounds, identified using spectroscopic methods such as FTIR, HRTOF-MS, 1D, and 2D NMR, and then compared to previously described compounds. Compounds 1-4 were identified as ergosterol (1), ergosterol peroxide (2), atroside (3), and cerevisterol (4). The four isolated compounds were evaluated for antibacterial activity against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 11229 and displayed activity with MIC50 values of 500 µg/mL. Keywords: Antibacterial activity; Chisocheton macrophyllus; Fusarium phaseoli; steroids.
Co-Authors Abigael, Reliana Ace Tatang Hidayat Ahmad Darmawan Andre A. Sonda Ba'u, Defrikson Desi Harneti Putri Huspa Edi Suryanto Edi Suryanto Erwin Sitorus Euis Julaeha Farabi, Kindi Ferry Ferdiansyah Sofian, Ferry Ferdiansyah Frenly Wehantouw Harry S. J. Koleangan Henry Aritonang Hilmayanti, Erina Johnly A Rorong Johnly A. Rorong Junito, Junito Khalijah Awang Khlaijah Awang Kindi Farabi Kindi Farabi Kindi Farabi Koleangan, Harry Steven Julius Lidya I. Momuat Lidya Irma Momuat Mantiri, Sisilia A Max R.J Runtuwene Max Revolta John Runtuwene Meiske S. Sangi Naini, Al Arofatus Novita Thaib, Novita Nur Insani Amir Nurlelasari Nurlelasari Paulina yamlean Pettanaga, Putri Firania Pollo, Hard Napoleon Rani Maharani Rani Maharani Salim, Muhammad Fajrin Saranaung, Andika Sari, Aprilia Permata Sinaga, Jonathan Cavin Ezra Sinaga, Siska Elisahbet Sofa Fajriah Sofa Fajriah Stevi G. Dungir Sujana, Kinaya Vizria Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Unang Supratman Vanda S Kamu Vanda S. Kamu Vanda Selvana Kamu Yenny Tambunan Yoshihito Shiono Yoshihito Shiono