Article Per Year (5 Year)
p-Index From 2021 - 2026
1.325
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Dasar e-Journal Pustaka Kesehatan Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Pharmaciana: Jurnal Kefarmasian Program Kreativitas Mahasiswa - Penelitian Indonesian Journal of Pharmaceutical Science and Technology STOMATOGNATIC- Jurnal Kedokteran Gigi Journal of Agromedicine and Medical Sciences Global Medical and Health Communication Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Ilmu Kefarmasian Indonesia Journal of Tropical Ethnobiology Journal of Bio-molecule Research And Engineering Journal of Agropharmacy Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)
Evi Umayah Ulfa
Fakultas Farmasi Universitas Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Dentistry Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Nursing Public Health

Published : 25 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 25 Documents
Search

 1   2   3 
Uji Aktivitas Antiplatelet Ekstrak Etanol Kubis Merah (Brassica oleracea var. capitata L.) Antiplatelets activity of red cabbage ethanolic extract (Brassica oleracea var. capitata L.) Rizki Rica Rachim Fadilah Putri; Evi Umayah Ulfa; Rini Riyanti
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Antiplatelet aggregation effect of red cabbage ethanolic extract (Brassica oleracea var. capitata L.) was measured by bleeding time on Balb-C mice. The test substances given orally during the period of 8 days. Bleeding time was measured on first day and 9th day. Red cabbage ethanolic extract were divided into 3 doses: wich are 9.69 mg/kg body weight; 19,38 mg/kg body weight, and 38,76 mg/kg body weight. After getting the percentage of bleeding time increase, data were analyzed by analysis of variance test (Anova), followed by individual comparison within the groups by LSD test. The test substances above respectively increased bleeding time 58 ± 16%; 70,7 ± 8,9 %, and 113 ± 12%. As the conclusion, red cabbage ethanolic extract doses 38,76 mg/kg body weight could increase bleeding time as well as positive controle 129 ± 8,2%. Keywords: antiplatelets, Brassica oleracea red cabbage, bleeding time.
Uji Aktivitas Anti Hiperurisemia Ekstrak n-Heksana, Etil Asetat, dan Etanol 70% Biji Jinten Hitam (Nigella sativa) terhadap Mencit Hiperurisemia (Anti Hyperuricemic activity of n-Hexane, Ethyl acetate, and Ethanol 70% of Black Cumin Seed (Nigella sativa) Umar Dian Prambudi Kusuma; Siti Muslichah; Evi Umayah Ulfa
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this study was to determine the in vivo anti-hyperuricemic effect of black cumin extract. Black cumin was extracted with n-hexane, ethyl acetate, and ethanol 70% continously. Fifteen mice were divided into five groups K-, K+, P1, P2, and P3. Group K- as negative control (chicken liver juice 0,2 % b/v) and group K+ as positive control (allopurinol 10 mg/kg b.w.). Group P1, P2, and P3 received n-hexane, ethyl acetate, and ethanol 70% black cumin extract. The extracts were given to mice in 200 mg/kg b.w dose for 4 days. The blood samples were taken an hour after potassium oxonate injection (300 mg/kg b.w) to inhibit uricase enzym. The data was collected and analyzed using one way ANOVA and LSD test. The result showed that activity of ethyl acetate extract of black cumin has no significant difference of possitive control. Based on phytochemistry screening it has terpenoid, saponin, and alkaoid. Terpenoid has anti-hyperuricemic effect but its mechanism still unknown.   Keywords: hyperuricemic, Nigella sativa, terpenoid.
Pengaruh Gel Binahong (Anredera cordifolia (Ten.) Steenis) terhadap Penyembuhan Luka Tikus Diabetes yang Diinduksi Aloksan (The Effect of Binahong (Anredera cordifolia (Ten.)Steenis) Gel on Wound Healing Process of Diabetic Rats Induced by Alloxan) Shinta Cholifatu Rohma; Evi Umayah Ulfa; Diana Holidah
Pustaka Kesehatan Vol 3 No 3 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes Mellitus (DM) is a chronic disease that can be indicated by the increase of blood glucose level. Diabetic ulcer is one of complication caused by DM. Binahong (Anredera cordifolia (Ten). Steenis) has a potential effect to heal the wound. The purpose of this study was to examined the effect of binahong gel on the wound healing process of diabetic rats induced by alloxan. The observation was done on the wound's histopathological preparation. The wound were made on diabetic rats, then the binahong gel at a dose of 100 mg and 200 mg were spread on to their wounds. The observation of qualitative descriptive parameters consisted of epithelial tissue, collagen, and new blood vessels formation. The results showed that binahong gel was able to accelerate the wound healing process of diabetic ulcer.   Keywords: Anredera cordifolia, diabetic ulcer, wound healing, alloxan, histopathological profile
Uji Aktivitas Antihiperurisemia Ekstrak n-Heksana, Etil Asetat, dan Etanol 70% Daun Tempuyung (Sonchus arvensis L.) pada Mencit Jantan Hiperurisemia Ferani Cendrianti; Siti Muslichah; Evi Umayah Ulfa
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this research was to study the activity of Sonchus arvensis leaves extract as antihyperuricemics agent on hyperuricemic male mice. Eighteen male mice were divided into six groups, i.e the first group as a normal control K(N); the second group as a negative control K(-) treated chicken liver juice 0,2 % b/v orally; the third group as a positive control K(+) treated allopurinol 10 mg/kg BW orally; the fourth to six groups P1, P2, and P3 were treated with nhexane, ethyl acetate, and ethanol 70% of Sonchus arvensis leaves extract 300 mg/kg BW orally for 4 days. Pottasium oxonate 300 mg/kg BW i.p was induced 1 hour before the blood samples was collected. The blood samples was taken from vena ophtalmicus. The concentration of uric acid was determined with FS DHBSA. Data was analyzed using one way ANOVA and LSD test. The result showed that activity of ethyl acetate and ethanol 70% extract of Sonchus arvensis leaves has significant effect in reducing the concentration of uric acid in hyperuricemic mice. Base on phytochemistry screening it has flavonoid and terpenoid. The substance that estimated has antihyperuricemic activity was flavonoid.
Deteksi Aktivitas Fibrinolitik Isolat Bakteri WU 021055* Asal Perairan Pantai Papuma Jember Menggunakan Zimografi Evi Umayah Ulfa; Esti Utarti; Izzay Afkarina; Sattya Arimurti; Kartika Senjarini
Global Medical & Health Communication (GMHC) Vol 5, No 2 (2017)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.707 KB) | DOI: 10.29313/gmhc.v5i2.1914

Abstract

Bakteri merupakan sumber penting berbagai enzim termasuk enzim fibrinolitik. Enzim ini diperlukan untuk mendegradasi bekuan darah pada orang yang mengalami penyakit trombosis. Isolat bakteri WU 021055* asal Pantai Papuma Jember terbukti menghasilkan enzim fibrinolitik ekstraseluler. Penelitian ini bertujuan mengetahui ukuran protein yang memiliki aktivitas fibrinolitik dan mengidentifikasi karakteristik morfologi isolat WU bakteri WU 021055*. Penelitian ini dilakukan di Laboratorium Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jember pada April–Agustus 2014. Aktivitas fibrinolitik presipitat protein (PP) ditentukan menggunakan metode fibrin plate agar dan zimografi fibrin. Ekstrak protein kasar (EPK) dipanen pada jam ke-12 dan dipresipitasi menggunakan amonium sulfat 80%. Hasil uji aktivitas fibrinolitik menggunakan fibrin plate agar menunjukkan presipitat memiliki aktivitas fibrinolitik lebih besar dibanding dengan EPK. Dari hasil karakterisasi PP menggunakan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) diperoleh 11 pita protein dengan ukuran 12–41 kDa. Berdasar atas hasil zimografi fibrin, pita protein dengan berat molekul 24 kDa yang memberikan aktivitas fibrinolitik. Protein dengan ukuran 24 kDa ini mampu mendegradasi substrat fibrin. Simpulan, isolat bakteri WU 021055* mengandung berbagai protein ekstraseluler, memiliki bentuk koloni bulat berwarna putih dan termasuk bakteri gram prositif berbentuk batang.DETECTION OF FIBRINOLYTIC ACTIVITY OF WU 021055* BACTERIAL ISOLATE FROM PAPUMA BEACH COASTAL JEMBER USING ZYMOGRAPHYBacteria were important resources for various enzymes including fibrinolytic enzymes. This enzyme is  capable of degrading fibrin clot in patient with thrombotic diseases. Bacterial isolate of WU 021055* from Papuma Beach Coastal Jember could secrete extracellular fibrinolytic enzymes. The objective of this reasearch was to determine the molecular weight of protein responsible for fibrinolytic activity and to identify morphologycal characterization of bacterial isolate of WU 021055*. This study was conducted at Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Universitas Jember in April–August 2014. Fibrinolytic activity of precipitate protein (PP) was determined by using fibrin plate agar and fibrin zymography. Crude protein extract (CPE) was harvested at 12 hours and precipitated by 80% ammonium sulphates. The result of fibrinolityc activity determination showed that fibrinolytic activity of PP was higher than CPE. Protein characterization of PP by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) obtained 11 different protein bands corresponds to value 12–42 kDa. Based on fibrin zymography, the 24 kDa protein might contribute to fibrinolytic activity due to degraded fibrin substrates. In conclusion, bacterial isolate of WU 021055* contained extracellular fibrin protein was white colony and gram positives bacilli able to degraded.
Uji Toksisitas Subkronis Ekstrak Kayu Kuning (Arcangelisia flava Merr) terhadap Hepar dan Ginjal Ema Rachmawati; Evi Umayah Ulfa
Global Medical & Health Communication (GMHC) Vol 6, No 1 (2018)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.375 KB) | DOI: 10.29313/gmhc.v6i1.2203

Abstract

Kayu kuning (Arcangelisia flava Merr) merupakan tanaman asli Indonesia yang banyak mengandung alkaloid berberin dan flavonoid. Tanaman ini telah terbukti memiliki aktivitas sebagai antioksidan, antibakteri, antikanker, dan antihiperlipidemia, namun belum diketahui pengaruhnya terhadap hepar dan ginjal sehingga penelitian ini bertujuan mengetahui pengaruh ekstrak A. flava terhadap parameter biokimia hepar, histopatologi hepar, dan histopatologi ginjal tikus. Penelitian dilakukan di Laboratorium Biomedik, Fakultas Farmasi Universitas Jember pada bulan Mei–Agustus 2016. Sebanyak 40 ekor tikus jantan berusia 2–3 bulan dengan bobot 200–300 gram dibagi menjadi 4 kelompok. Kelompok kontrol hanya diberi sodium-carboxymethyl cellulose (CMC-Na) 1%, sedangkan kelompok perlakukan diberi ekstrak A. flava dosis 250, 500, dan 750 mg/kgBB per oral selama 28 hari. Pada hari ke-28, dilakukan penentuan kadar SGOT, SGPT, serta pengamatan histopatologi hepar dan ginjal. Nilai SGPT menunjukkan tidak berbeda bermakna antara kelompok kontrol dan kelompok perlakukan (p<0,05). Hasil pengamatan histopatologi hepar menunjukkan pemberian ekstrak A. flava dosis 250, 500, dan 750 mg/kgBB per oral menyebabkan kongesti pembuluh darah hepar, namun tidak mengakibatkan nekrosis. Pengamatan histopatologi pada ginjal tidak terdapat peradangan, namun terdapat perdarahan sedikit pada glomerulus dan interstitial. Simpulan, pemberian ekstrak A. flava dosis 250, 500, 750 mg/kgBB selama 28 hari tidak mengakibatkan kerusakan hepar dan ginjal. SUBCHRONIC TOXICITY TEST OF YELLOW ROOT EXTRACT (ARCANGELISIA FLAVA MERR) ON HEPAR AND RENALKayu kuning (Arcangelisia flava Merr) is one of Indonesian herbal plant which contain berberine alkaloid and flavonoids. This plant has been proven as antioxidant, antibacterial, anticancer, antihiperlipidemic and antidiabetes. However, the effect of A. flava extract on hepar and renal has not been studied yet. Accordingly, the objective of this study was to evaluate the effect of A. flava extracts towards chemical parameters of hepar, histopathology of hepar and renal rats. This research was conducted in The Biomedic Laboratory, Faculty of Pharmacy of Universitas Jember on May to August 2016. Forty Wistar male rats aged 2–3 moths, weighed 200–300 grams were divided into four groups. Control groups were only given sodium-carboxymethyl cellulose (CMC-Na) 1% orally while tretment groups were given A. flava extracts at dose 250, 500, and 750 mg/kgBW orally for 28 days. On the day 28, the SGOT, SGPT were measured and histopathology of liver and renal were observed. The result of this research showed that SGPT value of control group and treatment group were not significantly different (p<0.05). Histopathogical evaluation of liver showed that oral administration of A. flava extract at dose 250, 500, and 750 mg/kgBW caused congestion of liver blood vessel, but the liver cell did not experience necrosis. Histopathogical observation on renal showed that there was no inflamation but there was a small bleeding in glomerolus and interstitial. In conclusion, the administration of A. flava extract at doze 250, 500 and 750 mg/kbBW for 28 days do not affect the liver and the renal.
Studi Etnofarmasi Suku Osing Kecamatan Kabat, Singojuruh dan Rogojampi Auralia Putri Pratama; Debi Listiayana; Dedi Irawanto; Jacinda Na’ilahafitra; Rismatul Khoiroh; Yuniatin Hasanah; Indah Yulia Ningsih; Evi Umayah Ulfa
Journal of Tropical Ethnobiology 2021: Prosiding Seminar Nasional PMEI V 2020
Publisher : The Ethnobiological Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Medicinal plants have been widely used for public health maintenance. Ethnic traditional medicine traditions can be used as a reference for the discovery of new medicines from natural ingredients. The Osing tribe that inhabits several areas in Banyuwangi Regency, including in Kabat District, Singojuruh District and Rogojampi District, has traditional medicine passed down by their ancestors and has not been well documented. The purpose of this ethnopharmaceutical study is to document the knowledge of traditional medicine of the Osing tribe and identify plant species used for medicine. Traditional medicine knowledge was carried out by semi-structural interviews using open-ended questions with 24 informants. Data were analyzed with the value of Used Value (UV) and Informant Consensus Factor (ICF). The results of the study recorded 30 types of plants, nine types of animals and four types of minerals used by the Osing tribe for treatment. Plants that have UV values above 0.5 are Curcuma longa (1,208), Stenosemia aurita (Sw) C. presl (0.625), and Moringa oleifera (0.625). The parts of the plant that were widely used were leaves (36.39%), rhizomes (20.65%) and fruit (18.68%). The highest ICF value in this study was conjunctivitis (0.8) with the medicinal plant used, namely Stenosemia aurita Sw C. Presl. This study shows that medicinal plants play a very important role in maintaining the health of the Osing people.
Penentuan Kadar Genistein dan Aktivitas Hambatan Tirosinase Kedelai (Glycine max) Terfermentasi Aspergillus oryzae ESTIKA YUNINDARWATI; EVI UMAYAH ULFA; ENDAH PUSPITASARI; MOCHAMMAD AMRUN HIDAYAT
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 1 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.688 KB)

Abstract

Tujuan dari penelitian ini adalah menentukan kadar genistein dan aktivitas hambatan dari ekstrak kedelai terfermentasi dan non-fermentasi. Kedelai difermentasi oleh Aspergillus oryzae selama 4 hari dan dimonitor kadar genistein serta aktivitas hambatan tirosinase setiap hari. Kadar genistein ditentukan menggunakan densitometer-KLT sedangkan aktivitas hambatan tirosinase dievaluasi menggunakan metode spektrofotometri. Hasil penelitian menunjukkan bahwa kadar genistein meningkat pada saat fermentasi hari ke-1 sampai ke-3 (45,645-1256,042 μg/g ekstrak) tetapi pada hari ke-4 kadarnya menurun (54,373 μg/g ekstrak). Aktivitas hambatan tirosinase juga mengalami peningkatan pada saat fermentasi hari ke-1 sampai ke-3 (dilihat pada nilai IC50) dan menurun saat fermentasi hari ke-4. Disimpulkan bahwa kadar genistein berkaitan dengan aktivitas hambatan tirosinase (R2 = 0,949).
Design and Cloning of Gene Encoding SLPI C-Terminal Domain in Escherichia coli TOP10 Evi Umayah Ulfa; Ni Nyoman Tri Puspaningsih
Indonesian Journal of Pharmaceutical Science and Technology Vol 9, No. 3, 2022
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v9i3.36918

Abstract

Elevated levels of neutrophil elastase in CPOD (Chronic Obstructive Pulmonary Disease) airways are regarded as the main trigger of lung destruction and inflammation. SLPI (Secretory leukocyte protease inhibitor), an inhibitor protease, represents an attractive candidate for treatment in chronic lung diseases due to proteases excess. The antiprotease active site of SLPI has been located on the C-terminal domain. This study aimed to design and clone the gene encoding the C-terminal domain of SLPI (SLPIc). The gene encoding SLPIc was optimized and predicted solubility using OptimumGene™ and SoDoPe software. The nucleotide sequence of the optimized SLPIc was synthesized, inserted into the pGEX 4T-2 vector commercially by Genscript, and transformed into the Escherichia coli TOP10. The pGEX 4T-2 vector contains a glutathione S transferase (GST) gene located before the MCS to generate a recombinant protein for fusion with GST. For purification purposes, the His-tag synthesized together with SLPIc. The optimized SLPIc nucleotide sequence gave a CAI value of 0.81, GC content 52.31, and a CFD of 2%. The solubility probability of SLPI fused with GST increased from 0.124 to 0.4656. Confirmation of the transformant using restriction and sequencing analysis showed that the gene encoding of SLPI domain C-terminal optimized in the pGEX 4T-2 plasmid was successfully transformed into E. coli TOP10 as novelty of this study. The optimized SLPIc gene in pGEX 4T-2 has a high probability of being expressed in E. coli based on in-silico analysis.
Cardioprotective Effect of Chloroform Extract of Arcangelisia flava on Doxorubicin-induced Cardiomyopathy Ramadhan, Andika Dewi; Baroroh, Rizqy Kiromin; Maharany, Dinda; Ulfa, Evi Umayah; Puspitasari, Endah
Jurnal ILMU DASAR Vol 23 No 1 (2022)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v23i1.23012

Abstract

Long-term use of doxorubicin as cancer chemotherapeutic agent would cause tissue toxicity, including cardiotoxicity. Arcangelisia flava is suggested to have cardio protective effect. This study aimed to determine the effect of chloroform extract of A. flava leaves on cardio histopathology of doxorubicin-treated Wistar white male rats. Wistar male rats were divided into four groups (1) control group; (2) doxorubicin 7.5 mg/kgBW intraperitoneally twice (day 1 and 6); (3) doxorubicin + chloroform extract of A. flava leaves 250 mg/kgBW/day orally for 11 days; (4) Chloroform extract of A. flava leaves 250 mg/kgBW/day orallly for 11 days. At the 12th day, the rats were sacrificed; the heart organ was taken to make histopathological preparations and analyzed using HE staining. Vacuolization and necrosis are the parameters used in evaluating this effect. The phytochemical screening was also done to determine the compounds in chloroform extract of A. flava leaves. Based on the HE staining, chloroform extract of A. flava leaves decreased the cardiotoxicity caused by doxorubicin. The phytochemical screening showed that chloroform extract of A. flava leaves contains flavonoid, tannin, alkaloid, and triterpenoid. The cardioprotective effect of chloroform extract of A. flava leaves was suggested to be contributed by the flavonoid, tannin, and triterpenoid.
Co-Authors Agustina, Bayu Agustina, Bayu Ajeng Maharani Sri Pananjung Ajeng Maharani Sri Pananjung, Ajeng Maharani Ameliana, Lidya Arafika, Whendy Waliya Auralia Putri Pratama Baroroh, Rizqy Kiromin Debi Listiayana Dedi Irawanto Desi Sandra Sari Dhani Wijaya, Dhani Diana Holidah Dianasari, Dewi Dwi Nurahmanto Ema Rachmawati Ema Rachmawati Endah Puspitasari Esti Utarti ESTIKA YUNINDARWATI Ferani Cendrianti Gazzali, Amirah Mohd Hidayatul Ulyah Imtiyaz, Andi Hilman Indah Yulia Ningsih Izzay Afkarina Jacinda Na’ilahafitra Kartika Senjarini Lina Winarti M Mirza Nuryady, M Mirza Maharany, Dinda Mochammad Amrun Hidayat Ni Nyoman Tri Puspaningsih Novanda asri I, Novanda asri Nuri . Pananjung A.S, Pananjung Paramayuda, Farukh Pratama P., M. Reynaldi Archan Putri Eka Maryani Ramadhan, Andika Dewi Rini Riyanti Rismatul Khoiroh Rizki Rica Rachim Fadilah Putri Rohmah, Etik Ainun Sattya Arimurti Shinta Cholifatu Rohma Umar Dian Prambudi Kusuma Yuniar Wahyu Rahmawati Yuniatin Hasanah Yunita Armiyanti