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Nihayah, Husnatun
Jurusan Biologi FMIPA Universitas Jember, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Struktur Histologi Trabekula Femur Mencit (Mus musculus L) Orkidektomi Pasca Pemberian Ekstrak tempe Kedelai Susantin Fajariyah; Siti Nafi’atul Muta’alimah; Eva Tyas Utami; Asmoro Lelono; Husnatun Nihayah
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.56668

Abstract

Testosterone deficiency is caused by, among other things, old age, chronic disease, and testicular cancer, which results in decreased bone density. Estrogen plays a more important role in the process of bone growth and resorption than the hormone testosterone. Therefore, to prevent a decrease in density in conditions of testosterone deficiency, it is necessary to administer external phytoestrogens, including tempeh, containing isoflavones deidzein dan genistein. The aim of this study was to determine the effect of administering tempeh extract on the histological structure of the femoral trabeculae of orchidectomized mice. Mice were divided into 4 groups, namely negative control (without orchidectomy), positive control (orchidectomy), and 2 orchidectomy treatment groups, followed by administration of tempe extract at a dose of 0.6 g/ml/day (D1) and 1.2 g/ml/day (D2). Tempeh extract was administered by gavage for 15 days after a 30-day healing period for bilateral orchidectomy. One day after the last administration of tempeh extract, the mice were anesthetized and dissected to remove the femur bones to make preparations using the paraffin method and HE staining. The parameters observed were the number of osteoblasts and osteoclasts and the thickness of the femoral trabeculae. Giving soybean tempeh extract at a dose of 0.6 mg/ml/day by gavage for 15 days can cause an increase in the number of osteoblasts and trabecular thickness, as well as reducing the number of osteoclasts in the femur of orchidectomized mice.
DHF Prevention Efforts in Sumbersari Jember: Assessment of Aedes aegypti Resistance Status to Deltamethrin Reni Puspitasari; Hidayatur Rohmah; Husnatun Nihayah; Purwatiningsih; Eva Tyas Utami; Susantin Fajariyah; Asmoro Lelono
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19883

Abstract

This study aims to determine the resistance status of Ae. aegypti to the deltametrin insecticide in Sumbersari Jember Village. A total of 100 2nd generation female Ae. aegypti were tested using the CDC bottle bioassay with a deltamethrin dose of 10 μg/ml and a diagnostic time of 30 minutes. Molecular detection using AS-PCR was also performed to detect kdr mutations at position 1016 of the VGSC gene. Mortality data was calculated using Ms.Excel with a confidence level of 95%. The molecular wight of the PCR product is calculated using a gel analyzer application.  The results of the CDC bottle bioassay show that the Ae aegypti population is still tolerant to the insecticide deltamethrin (91,25% in mortality rate). However, molecular analysis confirms the presence of a mutation at position 1016 of the voltage-gated sodium channel (VGSC) gene (homozygous mutation), which is known to be the primary target of pyrethroid insecticides. These findings indicate that although phenotypic resistance has not yet fully developed, the mosquito population has shown early indications of target-site insensitivity to deltamethrin, which has the potential to develop into resistance under sustained selection pressure. Further use of deltamethrin insecticide may potentially increase phenotypic resistance, namely a decrease in mosquito mortality after exposure to deltamethrin insecticide. Therefore, an integrated vector control strategy and routine monitoring of mosquito resistance status in the region are required.
Morphology and bioactive compounds of gurmar (Gymnema sylvestre) with potential as antidiabetics Yuliany, Neny Novita; Farhan, Abdillah Maulana; Ziyaroh, Putry Lazimatu; Dwinianti, Edia Fitri; Nihayah, Husnatun; Su'udi, Mukhamad
Kultivasi Vol 25, No 1 (2026)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v25i1.64921

Abstract

The medicinal plant Gymnema sylvestre is widely known for its ability to lower blood sugar levels. This study reviewed the physical characteristics and bioactive compounds of G. sylvestre sourced from different regions, emphasizing its potential as an antidiabetic agent. Morphological analysis includes leaf structure, trichome presence, and other anatomical features, which can influence the plant's chemical profile. Phytochemical analysis, performed using techniques like GC-MS, identifies key bioactive compounds such as saponins, flavonoids, and gymnemic acids, which contribute to its hypoglycemic effect. The findings indicate that regional variations can affect the concentration and presence of these compounds, suggesting that environmental factors and genetic diversity play significant roles. This comprehensive characterization supports the use of G. sylvestre as a natural antidiabetic treatment, which supports its potential as a source of antidiabetic-related bioactive compounds. Further research is recommended to explore the pharmacological mechanisms and optimize the extraction methods for maximum efficacy.
DNA barcode characteristics of Paphiopedilum supardii Braem & Loeb Mukhamad Su'udi; Vita Sindiya; Tri Ratnasari; Dhiyaul Kholis; Dwi Setyati; Husnatun Nihayah
Jurnal Ilmu Pertanian Indonesia Vol. 31 No. 3 (2026): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.31.3.571

Abstract

Paphiopedilum is a protected orchid (Orchidaceae) genus with the most endangered species due to its unique aesthetic qualities, totaling 15 species. The excessive exploitation of orchids has led to a decline in their natural populations. This has prompted comprehensive conservation efforts to preserve these species. However, it is quite challenging to identify orchids that have not yet bloomed because of the similar morphology of their vegetation among closely related species, necessitating alternative identification approaches that are both rapid and accurate, especially for endangered orchids. DNA barcoding is a rapid and accurate molecular method for species identification. In this study, the selected rare orchid was Paphiopedilum supardii Braem & Loeb. The research procedure included DNA sample isolation using a NEXprepTM Plant DNA Mini Kit, DNA amplification using rbcL and ITS primers, PCR product purification, and sequencing. Based on the research results, DNA samples were amplified using rbcL and ITS primers. The DNA band resulting from rbcL primer amplification was 600 bp in size, whereas the ITS amplification result was 900 bp. The PCR products were then purified directly using a Purification Kit (Jena Bioscience). The PCR and purification results were separated on a 1.25% agarose gel. Based on data analysis, BLAST results from NCBI and sequence alignment using MEGA11, Clustal X2, and GeneDoc showed that the rbcL sequence had a higher homology level than ITS. Therefore, the ITS sequence is more specific for use as a molecular marker in DNA barcoding research on P. supardii. Keywords: ITS, Molecular identification, P. supardii, rbcL
Co-Authors Budi Mulyaningsih Dhiyaul Kholis Dwi Setyati Dwinianti, Edia Fitri Eva Tyas Utami Farhan, Abdillah Maulana Helda Wika Amini Hidayat Teguh Wiyono Hidayatur Rohmah Hurin Nur Laili Lelono, Asmoro Mukhamad Su'udi Nailatur Rifdah Nurul Ulya Luthfiyana Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Puspitasari, Reni Reni Puspitasari Rike Oktarianti Rohmah, Hidayatur Siti Nafi’atul Muta’alimah Sitti Rahmah Umniyati Su'udi, Mukhamad Susantin Fajariyah Syarifah, Ratna Dewi Tri Ratnasari Vita Sindiya Yuliany, Neny Novita Ziyaroh, Putry Lazimatu