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All Journal Media Veteriner Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Journal of Veterinary and Animal Sciences
I Gusti Ketut Suarjana
Laboratorium Bakteriologi Dan Mikologi Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Pengobatan Penyakit Diare (Kolibasilosis) pada Babi dalam Upaya Meningkatkan Produkivitas Ternak di Desa Sudimara, Tabanan I.G.K. Suarjana; K. Tono P.G; N.K. Suwi; I.A.P. Apsari
Buletin Udayana Mengabdi Vol 15 No 1 (2016): Jurnal Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Have performed community service on Treatment of Diarrhea (Colibacillosis) In Pigs In Effort Improving Livestock Productivity in Sudimara village, Tabanan. This dedication is aimed at providing knowledge to the public about how to manage pigs intensively, ways of prevention and eradication of the disease in pigs kolibasilosis effectively and efficiently so as to maximize their production. Methods of service in the form of the action from home to home, especially to farmers whose pigs diarrhea, held a therapeutic action in the form of antibiotics, anthelmintic, vitamins and minerals and to provide education on how to prevent diarrhea in pigs. The results obtained as follows: the team held a service from house to house on seven breeder pigs reportedly suffering from diarrhea. Pig diarrhea ranging from 1-3 months of age and the amount treated as many as 65 individuals. Based monitoring and reporting team community service founded that on the seventh day after the treatment was given these pigs had not diarrhea or healthy.
PENINGKATAN EFEKTIFITAS PEMELIHARAAN SAPI BALI MELALUI PENERAPAN TEKNOLOGI PETERNAKAN PADA PUSAT PEMBIBITAN SAPI I.N.K. Besung; N.K. Suwiti; I.G.K. Suarjana; P. Suastika; N.N. Suryani
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Sapi bali merupakan merupakan sumber daya genetik sapi asli Indonesia. Keberadaannya sudah tersebar ke seluruh Indonesia, bahkan sampai ke negara Malaysia dan Timor Leste. Sebagai ternak potong, sapi bali terus ditingkatkan kualitas dan kuantitasnya dengan cara penerapan teknologi manajemen pembibitan maupun penggemukan yang diikuti dengan penanganan gangguan kesehatan hewan. Kegiatan program ini meliputi rekonstruksi kandang, pemilihan bibit yang unggul, penanganan kesehatan hewan, dan pengolahan limbah. Pakan yang diberikan berasal dari lingkungan sekitarnya yang ditambah dengan konsentrat, dedak dan mineral. Penerapan kegiatan ini akan menghasilkan komoditas peternakan sapi bali yang berkualitas, yang berdampak pada peningkatan pendapatan peternak dan memberikan berkontribusi pada percepatan pembangunan ekonomi nasional. Model peternakan ini dapat dijadikan pilot project bagi peternak disekitarnya.
PEMBERANTASAN PENYAKIT DAN VAKSINASI HOG CHOLERA PADA TERNAK BABI DI DESA KELATING TABANAN BERATA BERATA; IB. OKA WINAYA; IGK. SUARJANA; IB. KADE SUARDANA
Buletin Udayana Mengabdi Vol 7 No 2 (2008): Volume 7 No.2 – September 2008
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

ABSTRACT Swines are the most important livestock to Balinese people because raising the swines could analogized with save the money.. Beside that balines people considering swine livestock also have economically value for religious ceremonial. Field observation indicated that some disease can threating the swine, one of these is Hog cholera. Hog cholera is a highly contagious viral disease of swine that occur in acute, a subacute, a chronic or a persistent form. In Order to protect the Bali cattle against this disease need conducting regularly vaccination. By vaccination can improvement cattle immunity against this disease. The objective this services is reducing economic loss due to Hog cholera infection and also increased farmes an understanding to this disease. .
Analisis Gen Patogenik iroN Escherichia coli Penyebab Kolibasilosis pada Ayam Kampung Tania Ria Gunawan; I Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian colibacillosis is an infectious disease caused by avian pathogenic Escherichia coli (APEC). APEC ability to cause disease depends on many pathogenic factors, one of them is iroN pathogenic gene. This study purposed to find out iroN gene sequence in APEC in Bali. Two APEC isolates from free range chicken in Tabanan dan Badung has been used. The isolates have been been purified and were available at Laboratory of Veterinary Bacteriology, Faculty of Veterinary Medicine, Udayana University. Chelex 10%was used for DNA isolation. DNA amplification using published DNA primer has been conducted with polymerase chain reaction (PCR) method. The PCR product was sequenced at First Base Laboratories, Malaysia using Sanger’s Dideoxy Nucleotide Termination method. The iroN gene of both isolates can be analyzed and have 659 bp in length. Both were 100% homologous. Phylogenic test using 52 DNA sequence of iroN gene from Escherichia coli and other bacteria in the world was conducted in MEGA 5.2. All data have 24 polymorphic sites of nucleotide acid and eight polymorphic sites of amino acid. The iroN gene of Bali isolates was in the same group as iroN gene from Australia (MF174860) and Hong Kong (MF474175). This gene can be used as pathogenic marker of APEC in Indonesia.
Standarisasi Cemaran Mikroba Sampel Daun Pegagan sebagai Persyaratan Mutu Bahan Baku Sediaan Obat Luh Made Sudimartini; Genta Dhamara Adam Putranto; I Gusti Ketut Suarjana; I Made Merdana
Buletin Veteriner Udayana Vol. 14 No. 4 August 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.117 KB) | DOI: 10.24843/bulvet.2022.v14.i04.p01

Abstract

Pegagan is an excellent plant used as a raw material for traditional medicine. The bioactive content in pegagan leaves plays a role in the wound healing process, able to cure complex dermatitis in dogs, proven as neuroprotective, and can reduce oxidative stress of body cells. Many efficacies make pegagan leaves have the potential to be produced into various forms of medicinal preparation. This study will calculate the number of microbes’ contamination in pegagan leaves which will be used as various drug preparations and then compare it to the General Standard Parameters for Medical Plant Extracts of the Ministry of Health of Republic Indonesia in 2000 and Regulation of the Head of BPOM RI Number 32 of 2019. The examination of microbial contamination was carried out through the Total Plate Count and Yeast Fungus Test. The research data obtained were quantitative data that were analyzed by counting the number of microbes that grow on PCA and PDA after incubation at the appropriate growth temperatures. The results of this study show that the Total Plate Count from the sample was 3250 CFU/gr and the Yeast Fungus Test was 450 CFU/gr. The number of Total Plate Count has fulfilled the requirements for the Oral Drugs (£105 colony/gr) and required for Semi Solid Drugs (£107 colony/gr). The number of Yeast Fungus Test are required for Oral Drugs (£103 colony/gr) and required for Semi Solid Drugs (£104 colony/gr).
Angka Lempeng Total Bakteri dan pH pada Cairan Rumen Sapi Bali Jantan yang Dipotong di Rumah Pemotongan Hewan Pesanggaran Ni Putu Nicky Mirahsanti; I Gusti Ketut Suarjana; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p01

Abstract

Tujuan penelitian ini adalah untuk mengetahui pH dan angka lempeng total bakteri (ALTB) pada rumen sapi bali dengan materi penelitian berupa cairan rumen sapi bali jantan sebanyak 17 sampel. Penelitian ini merupakan penelitian observasional analitik pendekatan Cross Sectional dengan pengamatan terhadap angka lempeng total bakteri (ALTB) dan pH di dalam rumen. Data yang diperoleh selanjutnya disajikan secara deskriptif. Kemudian, perbedaan ALTB pada pH tertentu dianalisis dengan Independent Sample T- test. Hasil penelitian menunjukkan bahwa kisaran pH rumen sapi bali adalah 6,8 sampai 7 dengan rerata 6,4 ± 0,1 dan kisaran ALTB yaitu 32 x 103 sampai 171 x 103 CFU/ml dengan rerata 74 x 103 ± 47 x 103 CFU/ml. Tidak ada perbedaan yang signifikan antara ALTB pada pH 6,8 dan ALTB pada pH 7.
Jumlah Khamir pada Rumen Sapi Bali Ditinjau dari pH dan Bobot Badan Ike Siwi Widyaningtyas; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p03

Abstract

This study aims to determine the amount of yeast, rumen pH, and body weight of bali cattle. 17 samples were taken from the rumen fluid of bali cattle. This study was an analytic observational study with a cross sectional approach with observations on the number of yeast on rumen pH and body weight of bali cattle. The data obtained are then presented descriptively followed by regression correlation analysis. The results showed the average number of yeast in the rumen of bali cattle 21x103 ± 6.9x103 cells/ml, the average pH of rumen fluid of bali cattle 6.9 ± 0.07, and the average body weight of bali cattle 251 ± 47.8 kg. There is a significant difference between the number of yeast with the pH and body weight of bali cattle.
Isolasi dan Identifikasi Bakteri Shigella spp. Penyebab Diare pada Anjing Voni Cornelia Br Sembiring; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p08

Abstract

Dogs are one of the favorite animals that are kept by many people because they are considered intelligent and loyal animals. One of the main health problems of dogs is diarrhea caused by a bacterial infection. Shigella spp. is a pathogenic bacterium in the digestive tract of dogs. This study aims to isolate and identify Shigella spp. bacteria that cause diarrhea in dogs. Samples studied using rectal swabs in dogs showing clinical symptoms of diarrhea with a total of 48 samples from veterinary clinics in the city of Denpasar and its surroundings. Isolation of Shigella spp. was conducted on Eosin Methylene Blue Agar, Salmonella-Shigella Agar, and Sheep Blood Agar. Colonies that grew and separated on Salmonella-Shigella Agar medium were subjected to Gram staining, catalase test, oxidase test and continued with biochemical tests, namely Triple Sugar Iron Agar, Sulfid Indole Motility, Methyl Red Voges Proskauer, Simmons Citrate Agar, and test for sugars such as glucose, lactose, mannitol, and arabinose. The results showed that of the 48 isolated and identified samples, one sample showed a positive result for Shigella spp. with possibly the species Shigella dysentriae. It was concluded that one (2.08%) of Shigella spp. bacteria could be isolated and identified from 48 samples of dog rectal swabs that showed clinical symptoms of diarrhea. It is necessary to conduct further research on the level of pathogenicity of Shigella spp. bacteria and for further research, it is necessary to supplement the API 20E test to determine the Shigella species.
Isolasi dan Identifikasi Enterobacter spp. pada Anjing Diare Matilda Krisnawati; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p07

Abstract

This study aims to isolate and identify Enterobacter spp. from the digestive tract of dogs with diarrhea. Diarrhea is a digestive disease that commonly attacks dogs, one of which can be caused by Enterobacter spp. This study using 48 samples in the form of a dog with diarrhea rectal swabs from Denpasar and surrounding areas. The samples were isolated on Eosin Methylene Blue Agar, MacConkey, and Blood Agar media by streak method and then incubated for 24 hours at 37°C, then primary tests were carried out, namely gram staining, catalase test, and oxidase test, and identification tests were carried out on Triple media. Sugar Iron Agar, Simon Citrate Agar, Sulfide Indole Motility, Methyl Red Voges Proskauer, and sugar test. The results showed that two (4.16%) positive samples of Enterobacter spp. diarrhea in dogs. Therefore it can be concluded that Enterobacter spp. can be isolated and identified in the digestive tract of dogs with diarrhea. Howewer, it is necessary to carry out identification test for each Enterobacter species and more in-depth research on the pathogenicity of Enterobacter spp.
Uji Sensitivitas Bakteri Klebsiella spp. yang Diisolasi dari Anjing Kintamani Diare Terhadap Antibiotika Anak Agung Gede Agung Ananta Kusuma; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p07

Abstract

This study aims to determine the pattern of sensitivity of bacteria Klebsiella spp. isolated from kintamani dogs with diarrhea. The sample of this study were 4 isolates of Klebsiella spp. isolated from kintamani dogs with diarrhea. The method used in this study is the Kirby-Bauer disc diffusion method in duplicate using the antibiotics Kanamycin, Sulfamethoxazole-trimethoprim, and Streptomycin. The data obtained were expressed descriptively qualitatively by measuring the antibiotic inhibition zone formed. The results showed that isolates of Klebsiella spp. 75% intermediate (3 isolates) and 25%(1 isolate) sensitive to Kanamycin, all samples sensitive to Sulfamethoxazole-trimethoprim and 75% (3 isolates) sample intermediate and 25% (1 isolate) sensitive to Streptomycin.
Co-Authors Agnes Endang Tri H Ahmad Nuzuludin Kadri, Ahmad Nuzuludin Aida Lousie Tenden Rompis Anak Agung Gede Agung Ananta Kusuma ANITA KAROLINA SIMAMORA BERATA BERATA Bianca Violanda Junus Bolla, Nelci Elizabeth chyntia nirmalasari mantrawan Daniel Opristanta Barus Danu Suprayogo Dwi Arso Purba Dwi Astalia Zuanita Eggy Hidta Lusandika Fanayoni, Aditana Genta Dhamara Adam Putranto HERTATI ANRIANI LUBIS I Gede Eka Chandrawan I Gusti Ngurah Kade Mahardika I Kadek Juli Handriana I Ketut Berata I Ketut Suada I Made Adhi Kusuma Dwipayana I Made Kardena I Made Merdana I Made Sukada I Nengah Kerta Besung I Putu Yasmanta Primarta Putra I Wayan Aris Suharsa I Wayan Suarnata I.A.P. Apsari IB. KADE SUARDANA Ida Bagus Oka Winaya Ike Siwi Widyaningtyas Indra Manik Pradipta Indra, Rusmin Iwan Harjono Utama JERRY BIROWO K. Tono P.G Kadek Andre Sulaksana Kadek Satria Adi Marhendra KARTINI HUTASOIT Ketut Tono Pasek Gelgel Leny Beatry Veronica Luh Made Sudimartini M Hasan Isnan Made Deddy Dharmana Putra Made Tubagus Dharmajaya Magfirah Syahruddin Makselina Ayu Dwi Purwanti MARGARET ANASTASIA MANULLANG Marlissa, Faccettarial Cylon Marchel MAS DJOKO RUDYANTO Matilda Krisnawati Mochammad Imron Awalludin MUHAMMAD RHIYAN SAKTI N.K. Suwi N.N. Suryani Nazara, Agustina Lesmauli Ni Kadek Meita Swandewi Ni Ketut Suwiti Ni Putu Nicky Mirahsanti Nyoman Anandiya Ramaditya P. Suastika Paramita, Putu Wahyuni Pratanto, Aditya Putri Udayani Putu Henrywaesa Sudipa Ratna Pandu Finata Septian Naria Rusmaniarno Sousa, Rojelio Dias Trindade Tania Ria Gunawan Trisno, Komang Voni Cornelia Br Sembiring Wati, Ni Komang Desi Yena Widya Asmara Yuniarti Sasmita Yusmaniar Galuh Adi Luhung