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Journal : Rumphius Pattimura Biological Journal

ADMINISTRATED OF SNAKE WOOD (Strychnos lucida) TO DECREASED ABNORMALITY SPERMATOZOA OF MICE (Mus musculus) DIABETES MELLITUS MODELING Kunu, Marissa Beatrix; Baszary, Chomsa Dintasari Umi; Killay, Amos
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p037-042

Abstract

Diabetes mellitus, known as diabetes or blood sugar disease, is a group of chronic diseases characterized by increased blood sugar level (hyperglycemia). Prolonged hyperglycemia conditions will cause an increase in ROS (Reactive Oxygen Spesies) production by the mitochondria, which then cause damage to the mitochondrial membrane resulting in loss of function of the mitochondrial membrane potential and can induce sperm cells apoptosis. This study aims to determine effect of kayu ular (Strychnos lucida) steeping on decreasing spermatozoa abnormalities of mice (Mus musculus) diabetes mellitus model. The result showed that administration of kayu ular steeping at concentration of 2,6gr/50ml was signicantly reduced (P<0.05) spermatozoa abnormality. This dose is the most effective concentration to decreased the spermatozoa abnormality of mice. This though to be due to the active compounds found in steeping kayu ular (Strychnos lucida) which can counteract free radicals due to diabetes mellitus that decreasing spermatozoa abnormalities.
SOATTING OF UREA FERTILIZER AND CONCENTRATION LEVEL OF SEAWEED CARRAGINAN Eucheuma cottonii Rijoly, Stefano; Killay, Amos; Rupilu, Joseph A
RUMPHIUS Vol 2 No 1 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i1p025-031

Abstract

Euchema cottonii seaweed as carrageenan-producing, growth and development is in need of the quality of light and nutrients such as nitrogen, such as urea. This study aims to determine the combination of long soaking and urea concentration levels that are effective in improving the content of Euchema carrageenan cottonii. This research method uses long immersion urea treatment 2 hours, 4 hours, 6 hours and without immersion (control) and urea concentration of 1 gram, 1,5 gram, 2 gram and wihout of urea fertilizer (control), repeat 3 times. The content of carrageenan in each treatment will be analyzed using ANOVA, followed by HSD test at 95% significance level. The results showed that the best carrageenan content (66.52%) in the treatment of long soaking 2 hours with 2 grams of urea concentration and carrageenan content of the lowest (51.33%) obtained in the treatment of long immersion 6 hours with 1 gram of urea concentration. Carrageenan seaweed Eucheuma cottonii on research generally meets the standards of quality carrageenan for maximum moisture content 35% (SNI Indonesia), and ash content of 15-40% (FAO, FCC), a maximum of 35% (EEC).