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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Ilmu Ternak JURNAL ILMU DAN TEKNOLOGI PETERNAKAN JURNAL SAINS DAN TEKNOLOGI INDONESIA Animal Agricultural Journal Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of the Indonesian Tropical Animal Agriculture JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Bulletin of Applied Animal Research Jurnal Teknologi Pangan Livestock and Animal Research

Edy Kurnianto

Faculty Of Animal And Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang, 50275, Indonesia

Author-ID : 301480

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Immunology & microbiology Veterinary Other

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Keragaman genotipik entok (Cairina moschata) berdasarkan polimorfisme protein darah Sutopo Sutopo; Dela Ayu Lestari; Asep Setiaji; Edy Kurnianto
Livestock and Animal Research Vol 19, No 3 (2021): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Objective: The aim of this study was to identified genetic diversity of muscovy duck in Central Java through blood protein polymorphisms by using electrophoresis method.Methods: Blood sample was collected from a total of 60 muscovy ducks from the districts of Demak, Magelang and Pekalongan, 20 samples each. The Electrophoresis Polyacrylamide Gel of Electrophoresis–Thin Layer Electrophoresis (PAGE-TLE) was used in this study. Parameters observed were protein of albumin (Alb), ceruloplasmin (Cp), transferrin (Tf) and amylase-I (Am-I). Gene frequency, heterozygosity (Ho) and genetic distance were analyzed by using DISPAN program.Results: The results showed that the Alb, Cp, Tf and Am-I of muscovy duck showing polymorphic characters. Gene frequency of AlbB (0.61) was higher compared to AlbA (0.39). Gene frequency of CpA (0.62) was higher than the CpB (0.38). Furthermore, gene frequency of TfA and TfB were 0.32 and 0.68, respectively as well as gene frequency of Am-IA (0.82) was higher than Am-IB (0.18). Muscovy duck population of Demak showed higher Ho value for Alb and Am-I, whereas Magelang showed higher value for Cp and Tf. Population of muscovy duck of Demak showed closer genetic distance to Pekalongan than Magelang.Conclusions: The protein blood locus of three population of muscovy duck in Central Jawa showed polymorphic characters.

Genetic diversity of Sragen Black Cattle based on D-Loop gene sequencing analysis Rahayu Kusumaningrum; Sutopo Sutopo; Edy Kurnianto
Livestock and Animal Research Vol 18, No 2 (2020): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Objective: The objective of this study was to investigate the genetic diversity of Sragen Black Cattle based on D-loop sequences analysis.Methods: A total of 25 blood samples belonged to Sragen Black Cattle that had no genetic relationship within sample. The DNA genome was extracted based on the manufacturer’s standard protocol using gSYNC DNA Mini Kit (Geneaid Biotech Ltd.). D-loop gene was amplified using specific primer forward: 5’- TAGTGCTAATACCAACGGCC-3’ and reverse: 5’- AGGCATTTTGAGTGCCTTGC-3’ and then was sequenced. The sequencing result was aligned and analyzed by Molecular Evolutionary Genetics Analysis (MEGA) version 6.0 to reveal genetic distance and phylogenetic tree. Genetic diversity and haplotype were analysed by DNA Sequence Polymorphism (DnaSp) v6.12.03.Results: The results revealed that there were 11 haplotypes with Pi = 0.00675±0.00201 and Hd = 0.767±0.086. Sragen Black Cattle was divided by two cluster in phylogenetic tree with average of genetic distance was 0.0032.Conclusions: In conclusion, all of Sragen Black Cattle are on the same cluster and have closer genetic relationship to Bos indicus rather than Bos taurus with similarity level 85.76 % based on BLAST program.

Perbedaan Morfometri Kelinci New Zealand White Pada Jenis Kelamin dan Ketinggian Tempat yang Berbeda Nur Wakhid Salamudin; Edy Kurnianto; Sutopo Sutopo; Asep Setiaji
Bulletin of Applied Animal Research Vol 4 No 2 (2022): Bulletin of Applied Animal Research
Publisher : LPPM Perjuangan University of Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/baar.v4i2.956

Penelitian ini bertujuan untuk menganalisis keragaman morfomtrik kelinci New Zealand White berdasarkan se dan ketinggian tempat. Bahan yang digunakan dalam penelitian ini adalah 200 ekor kelinci dengan umur bulan lebih. Penelitian ini dilakukan di lokasi dengan ketinggian yang berbeda yaitu pada dataran rendah di wilayah Pleret Bantul Yogyakarta dengan ketinggian 0-250 meter di atas permukaan laut, pada dataran sedang di kabupaten magelang, Kendal dan Semarang dengan ketinggian 250-750. meter di atas permukaan laut dan pada ketinggian yang terletak di daerah temanggung dengan ketinggian lebih dari 750 meter di atas permukaan laut. Parameter yang digunakan adalah panjang kepala, lebar kepala, panjang telinga, lebar telinga, lingkar dada, dada bagian dalam, lebar dada, panjang tulang femur, panjang ulna-radius, panjang tulang tibia, dan panjang badan. Data dianalisis dengan desain klasifikasi two way dan uji rata-rata Tukey. Hasil penelitian menunjukkan bahwa terdapat perbedaan ukuran tubuh yang signifikan (P<0,05) antara jantan dan betina, yaitu pada lebar kepala, panjang telinga, lebar telinga, radius ulna panjang, dan panjang tulang skapula. Terdapat perbedaan (P<0,05) ukuran tubuh kelinci di dataran rendah, dataran sedang, dan dataran tinggi, tose terdapat pada lebar kepala, lebar telinga, lingkar dada, lebar dada, tampilan radius ulna, tulang skapula panjang, dan lebar pinggul sedangkan pada variabel panjang kepala, panjang tulang femur, panjang tulang tibia, dan panjang badan tidak menunjukkan perbedaan yang mencolok. Kesimpulan dari penelitian ini adalah perbedaan ukuran tubuh yang signifikan antara jarak pandang dan ketinggian tempat. Kata kunci : New Zealand White, kelinci, keanekaragaman morfometrik

Studi Apoptosis pada Daging Itik dan Ayam melalui Perubahan pH Lumbantoruan, Julia Ester; Setiani, Bhakti Etza; Al-Baarri, Ahmad Ni'matullah; Sutopo, Sutopo; Kurnianto, Edy
Jurnal Teknologi Pangan Vol 8, No 2 (2024)
Publisher : Program Studi Teknologi Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jtp.2024.20452

Apoptosis merupakan kematian sel yang terjadi saat pengkonversian daging yang berkaitan erat dengan kualitas daging. Salah satu parameter studi apoptosis adalah perubahan pH. Analisis pH dilakukan dengan menggunakan pH meter. Penelitian ini bertujuan untuk menganalisis nilai pH pada daging itik dan ayam yang disimpan selama 8 jam pada suhu ruang. Berdasarkan penelitian ini dapat diketahui bahwa nilai pH pada daging itik secara berturut-turut sebesar 6,70±0,21; 6,66±0,13; 6,64±0,31; 6,51±0,25 dan 6,50±0,19 sedangkan pada daging ayam sebesar 5,66±0,16; 5,59±0,11; 5,56±0,25; 5,52±0,16 dan 5,45±0,16. Penyimpanan daging pada suhu ruang menyebabkan penurunan nilai pH daging itik sebesar 0,20% dan daging ayam sebesar 0,21%.

Genetic Diversity of Simpo and Limpo Cattle Based on Five Blood-protein Locus Polymorphism Lestari, Dela Ayu; Sutopo, Sutopo; Setiaji, Asep; Kurnianto, Edy
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 3 (2022): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v9i3.25096

Simpo and Limpo cattle is a crossbred cattle of Indonesian local cattle and exotic cattle that popular among traditional farmers in Indonesia. The objective of this study was to identify genetic diversity of Simpo and Limpo cattle based on blood-protein polymorphism. A total of 70 blood samples were collected from unrelated 35 heads of Simpo cattle and 35 heads of Limpo cattle from Grobogan dan Kendal population. The blood plasm was used to identify the blood proteins. Blood protein analysis was performed using Sodium Dodecyl Sulfate-Polyacrilamide Gel Electrophoresis (SDS-PAGE). Observed bands were used to perform allele interpretation of five locus, namely Pre-albumin (Pe-Alb), Albumin (Alb), Post-albumin (Po-Alb), Ceruloplasmin (Cp) and Amylase-1 (Am-1). Results showed there were polymorphism in all of the five blood protein locus in both cattle; 4 of 5 and 2 of 5 of the blood-protein locus studied showed deviation from Hardy-Weimberg Equilibrium (HWE) for Simpo and Limpo cattle, respectively and the average heterozygosity value (H) was 0.478 and 0.362 for Simpo and Limpo cattle, respectively. In conclusion, the study indicated that population of Simpo and Limpo cattle has high genetic diversity. Keywords: allele frequency; genotype frequency; heterozygosity

HUBUNGAN SIFAT KUANTITATIF DAN REPRODUKSI SAPI PERANAKAN ONGOLE DI KABUPATEN REMBANG Indahwati, Ahyuni; Kurnianto, Edy; Setiatin , Enny Tantini; Samsudewa, Daud
Jurnal Sain Peternakan Indonesia Vol 19 No 4 (2024)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.19.4.227-233

This study aimed to analyze the relationship between quantitative traits and reproduction traits of Ongole grade cows in Rembang Regency. This study was done from May to July 2022. The number of samples used was 224 Ongole grade cows that have given birth at least once. Quantitative traits were obtained by measuring body condition scores (BCS), body length (BL), body height (BH) and chest circumference (CC). The class was based on Indonesian National Standards for Ongole grade cow breeding cattle in 2015 (Class I, II, III and Non-Class). The BCS used is the American system for beef cattle (score 1 to 9). The BL and BH were measured with a measurement stick. The CC was measured using the weight body Round-O (WB Round-O). The reproductive traits measured included service per conception (S/C), calving interval (CI), days open (DO) and estrus postpartum (EPP), which were obtained by observing cattle and interviewing breeders and inseminators. The analysis used was descriptive statistical analysis with averages and percentages and non-parametric analysis of Spearman’s correlation test. This research concludes a significant relationship between quantitative traits and S/C with a very weak negative direction and strength of the relationship.

Performa Pertumbuhan Tiga Varietas Ayam Kedu Umur 0–10 Minggu Akramullah, Muh; Kurnianto, Edy; Lestari, Dela Ayu; Setiatin, Enny Tantini; Setiaji, Asep
Jurnal Sain Peternakan Indonesia Vol 20 No 3 (2025)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.20.3.123-127

This study aimed to evaluate the growth performance of Red-Combed Kedu (RCK), Black-Combed Kedu (BCK), and White Kedu (WK) chickens based on sex during the early growth phase (0 to 10 weeks of age). A total of 136 Kedu chickens were raised under uniform management conditions, consisting of 99 RCK (65 roosters and 34 hens), 29 BCK (5 roosters and 24 hens), and 8 WK (6 roosters and two hens). Body weight was recorded weekly and analysed using a two-way General Linear Model (GLM) in SAS to assess the effects of chicken variety, sex, and their interaction. The Shapiro–Wilk test confirmed that the data were normally distributed (p > 0.05). The results showed that sex had a significant effect on body weight from weeks 3 to 10 (p < 0.05), with roosters consistently exhibiting higher weights than hens. Significant differences among chicken varieties were observed only in the hen group at weeks 9 and 10, where BCK hens had higher body weights (p < 0.05) than WK hens, while RCK hens showed intermediate values. In contrast, no significant differences were found among rooster groups at any age. These findings suggest that genetic factors play an essential role in influencing growth performance in Kedu chickens, particularly in hens. This information provides a valuable foundation for breeding selection and the conservation of local chicken genetic resources.

Co-Authors Agus Tri Sudaryanto Akramullah, Muh Al-Baarri, Ahmad Ni'matullah Argi Argiris Arnia Septarosa Dewi Asep Setiaji Bhakti Etza Setiani Choirunnisa Yuniarinda Daud Samsudewa Dela Ayu Lestari, Dela Ayu Dwi Purwanti Dwi Wijayanti Dwi Wijayanti Edjeng Suprijatna Enny T. Setiatin Enny Tantini Setiatin Enny Tantini Setiatin Galih Dewa Brata H. I. Wahyuni Herumawati, Widya Lyly I Ketut Gorde Yase Mas, I Ketut Gorde Indahwati, Ahyuni Julia Ester Lumbantoruan Luh Made Indah Sri Handari Adiputra Lumbantoruan, Julia Ester Muhammad Rivai Mukh Arifin Nur Wakhid Salamudin Rachmat Heru Sulaksono Rahayu Kusumaningrum Seno Johari Setiaji, Asep Setiatin , Enny Tantini Siswanto Imam Santoso Siti Hidayati Siti Zamhariroh Sri Kismiati Sutopo Sutopo Sutopo Sutopo Sutopo Sutopo Yon Soepri Ondho Yon Supri Ondho Yuni Widiarti Yuni Widiarti

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