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All Journal JMLS: Journal of Medical Laboratory and Science Current Biomedicine Mukhtabar: Journal of Medical Laboratory Technology Jurnal Kesehatan Siliwangi Jurnal Laboratorium Khatulistiwa Jurnal Pengabdian Masyarakat Kesehatan Indonesia
Iis Kurniati
Politeknik Kesehatan Jurusan Analis Kesehatan, Departemen Kesehatan Provinsi Jawa Barat, Babakan Loa Cimahi Utara
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Confirmation of Mycobacterium tuberculosis culture results with Ziehl-Neelsen staining and MPT64 antigen test Indrasari, Witri; Kurniati, Iis; Dermawan, Asep; Sufa, Hafizah Ilmi
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.11

Abstract

Background Culture of Mycobacterium tuberculosis (MTB) using egg-based solid media like Lowenstein Jensen (LJ) is the gold standard for tuberculosis diagnosis but requires extended incubation time. Rapid diagnostic tests, such as Ziehl-Neelsen (ZN) staining and the MPT64 antigen rapid test, are essential for early detection. Objective This study aimed to evaluate the effectiveness of ZN staining and the MPT64 rapid test in detecting MTB and Mycobacterium other than tuberculosis (MOTT) during different culture times. Methods Using a cross-sectional design, 110 culture-positive samples were analyzed from Hasan Sadikin Hospital Bandung over two months. Specimens were cultured on LJ media for eight weeks, with weekly observation of colony growth. ZN staining and MPT64 tests were performed on growing colonies. Results Less than four weeks culture, 61 samples (55.5%) were culture-negative, 45 (40.9%) were positive for MOTT, and the remainder were contaminated. In more than four weeks culture, 48 samples (43.6%) were positive for MTB, 45 samples (40.9%) were positive for MOTT, and 13 (11.8%) were culture-negative, and the remaining were contaminated. ZN-positive and MPT64-negative results indicated MOTT in less than four weeks culture, while ZN-positive and MPT64-positive results indicated MTB in more than four weeks culture. Conclusion While ZN staining was positive for both MTB and MOTT colonies, the MPT64 rapid antigen test was specific for MTB, supporting its use in confirming MTB detection alongside culture methods.
PEMBERDAYAAN MASYARAKAT UNTUK PENCEGAHAN PENYEBARAN TUBERKULOSIS DI DESA TANIMULYA KECAMATAN NGAMPRAH KABUPATEN BANDUNG BARAT DENGAN MENERAPKAN TEKNOLOGI PEMBUATAN HANDSANITIZER BERBAHAN DASAR DAUN SELEDRI (Apium graveolens L.) Marliana, Nina; Kurniati, Iis; Abror, Yogi Khoirul; Patria, Cecep
Jurnal Pengabdian Masyarakat Kesehatan Indonesia Vol. 4 No. 2 (2025): Jurnal Pengabdian Masyarakat Kesehatan Indonesia In Progress
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jpmki.v4i2.4218

Abstract

TB remains a global problem. In our country, TB prevention, including thorough handwashing, is a fairly effective health protocol for preventing TB transmission. Hand sanitizer is an alcohol-based hand cleaning liquid used to reduce contaminating bacteria by up to 99.9% by using it without rinsing with water. This liquid contains various ingredients that very quickly kill bacteria on the skin of the hands. Hand sanitizer aims to remove dirt and flora that stick to our hands. Celery (A. graveolens L.) is a type of vegetable plant with a menthol and distinctive celery aroma. Usually, celery is only used as a complementary ingredient in food preparations, but it turns out that celery contains several beneficial compounds such as flavonoids, saponins, tannins, essential oils as antibacterials. Therefore, celery can be used as a hand sanitizer. The practical goal of this PPDM is for the community in Tanimulya Village, Ngamprah District, West Bandung Regency to process celery for use as a hand sanitizer. The method offered is technology transfer through counseling, training, and mentoring. The target of the PPDM program was Tani Mulya Village in West Bandung Regency, given the dense population and high pollution in the area, necessitating hand sanitizer to prevent the spread of tuberculosis through the hands. The results of the PPDM program in Tani Mulya Village showed an increase in community knowledge and skills, as evidenced by the pre- and post-test results. The average pre-test score was 66.2, while the post-test score increased to 75.7.
Co-Authors Abror, Yogi Khoirul Alfi Agilia Saputri, Nianda Dermawan, Asep Feisal, Sonny Fuji Lestary, Rizka Ghaisani, Syifa Marha Habibah, Firda Nurul I Gede Sugiyanta Iin Nur Indra, Asep Ilmi Sufa , Hafizah Ilmi Sufa, Hafizah Indrasari, Witri Ismi Yulandari, Siti Linda Triana Mamat Rahmat Merdekawati, Fusvita Mulia, Yuliansyah Sundara Nina Marliana, Nina Patria, Cecep Prananda, Crystian Dwi Rahma Kurnia Sri Utami Ratnawaty, Gervacia Jenny Riyani, Ani Sari, Emilda Solihat, Mohamad Firman Sriwardani, I Dewa Ayu Sufa, Hafizah Ilmi Sulaeman Sulaeman Syafitri, Andita Izmi Yuliana, Elviani Mutiara Hoy Zuri Rismiarti