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All Journal Jurnal Sain Veteriner Indonesian Journal of Biotechnology Journal of Health (JoH) Indonesian Journal of Chemistry CHEESA: Chemical Engineering Research Articles Jurnal Lentera Jurnal Kedokteran Hewan
Asmarani Kusumawati
Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Indonesian Journal of Biotechnology

 1 
Characterization of envelope-transmembrane Gene of Jembrana Disease Virus Tabanan 1995 Isolate Kusumawati, Asmarani; Pratiwi, Rarastoeti; Astuti, Pudji; Hamid, Penny Humaidah
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

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Abstract

The availability of specific and rapid detection methods is essential for monitoring the health status of farmed species, particularly in viral disease as in this case early diagnosis is a critical factor in containing disease outbreaks. Jembrana Disease Virus (JDV) is a lentivirus that causes an acute, severe disease syndrome in infected Bali cattle in Indonesia, resulting in heavy economic losses because of the high mortalities. The virus-host interaction and the modes of transmission are still unknown. The goal of the research was to designa probe candidate of Jembrana Disease Virus based on envelope-transmembrane (env-tm) gene to optimize Jembrana disease detection method. The DNA fragment derived from env-tm of JDV was used, cloned in pGEX-TM and expressed in E.coli DH 5α. Sequence analysis was conducted with BLAST programs from NCBI. Sequence analyses of the fragments of env-tm clone, indicated that it has a very closed genetic relation with 97,68% homology identity. Probe was designed based on the conserved region of env-tm using Geneious resulted in JT2 252 bp long. BLAST analyses showed that probes had high specifity to other strains of JDV in Indonesia.Key words : probe, env-tm, JDV, specifity, sensitivity.
DIGoxigenin (DIG) Labeled Probe Candidate of Surface Antigen 1 (SAG1) for Toxoplasma gondii Detection Kusumawati, Asmarani; Septiana, Nafratilova; Hartati, Sri
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

Toxoplasma gondii is one of the opportunistic pathogen that causes toxoplasmosis. Infection of Toxoplasma gondii has been estimated as high both in human and animal. The manifestation of infection were abortion, hydrocephalus, brain calcification, chorioretinal scar, and loss of productivity even to death in patients with acquired immunosuppression. Early diagnostic method which are rapid and accurate is essential for T. gondii detection because of its high prevalence. The purpose of this study was to develop a sensitive probes derived from Surface Antigen 1 (SAG1) for detection T. gondii and to examine the specificity and sensitivity of probe as diagnostic tool for toxoplasmosis. This research used SAG1 gene of T. gondii local isolate IS-1 that was cloned into pGEX-2T and transformed into Eschericia coli DH5α. The sequence of SAG1 was labeled with DIGoxigenin (non radioactive labeled) using PCR DIG Labeling Mix to derive 213 bp (probe-TS). BLAST and dot-blot hybridization analyses showed that probes had high specifity with other strains of T. gondii. Probe was able to detect T. gondii DNAup to 10 ng/μl of total sample DNA.
Co-Authors A.A. Ketut Agung Cahyawan W Agung Budiyanto Anis Karuniawati Bambang Sumiarto Dini Wahyu Kartika Sari Dwi Prihantoko, Kurniawan Dwi Priyo Widodo, Dwi Erif Maha Nugraha Setyawan Fahhlevi, Nabilah Adzra Fani, Rifia Tiara fatimah Fatimah Harto Widodo, Harto Khairun Nissa Maulina, Nurina Tahta Afwi Nafratilova Septiana, Nafratilova Ni Wayan Kurniani Karja Penny Humaidah Hamid, Penny Humaidah Pratiwi Sudharmono, Pratiwi Priyo Jr, Topas Wicaksono Purnamasari, Elly Widyarni Eka Rahma Diyan Martha Rarastoeti Pratiwi raudatul jannah Sari, Dini Wahyu Kartika Sri Hartati Surya Agus Prihatno Susan M. Noor, Susan Tenri Ashari Wanahari Unsunnidhal, Lalu Wibowo, Syahputra Yuniar, Resa Miftahatu