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CORRELATION BETWEEN PRESEPSIN AND SEQUENTIAL [SEPSIS-RELATED] ORGAN FAILURE ASSESSMENT (SOFA) SCORE AS AN ORGAN DYSFUNCTION MARKER IN SEPSIS Stevi Dwiyani; Agnes Rengga Indrati; Leni Lismayanti; Adhi Kristianto S
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 1 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i1.1490

Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. The latest consensus in 2016 (Sepsis-3) identified organ dysfunction as an acute change in total SOFA score ≥2 points. An ideal laboratory examination is expected to detect sepsis in an early stage and correlated with the degree of infection. Presepsin or Soluble Cluster of Differentiation 14 Sub Type (sCD14-ST) is a proteolysis product of CD14 that is produced in 1-2 hour after innate immune activation during infections. The aim of this study was to determine the correlation of presepsin and SOFA score as an organ dysfunction marker in sepsis. This research was an observational, analytical cross-sectional study conducted in the Dr. Hasan Sadikin Hospital (RSHS) Bandung from September 2016 until July 2017. The subjects were 42 patients from the Emergency Department diagnosed as sepsis by clinicians using criteria of SOFA score ≥2 points. The serum sample was collected and measured for presepsin concentration. A correlation test was analyzed with Spearman analysis. This study showed the increasing of presepsin concentration associated with SOFA score (p=0.000; r=0.660). There was a positive correlation between presepsin and SOFA score as an organ dysfunction marker

DIFFERENCES OF ASYMMETRIC DIMETHYL ARGININE LEVEL IN PATIENTS WITH DIABETIC NEPHROPATHY AND NON-DIABETIC NEPHROPATHY Nita Elvina Wisudawati; Coriejati Rita; Leni Lismayanti; Adhi Kristianto Sugianli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 3 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i3.1420

Endothelial dysfunction occurs early in Diabetic Nephropathy (DN), characterized by elevated Asymmetric Dimethylarginine (ADMA) levels. Increased ADMA levels may inhibit endothelial Nitric Oxide Synthase (eNOS) production which are required for Nitric Oxide (NO) formation. Decreased NO levels can increase peripheral resistance and exacerbate the endothelial dysfunction. By knowing the difference of ADMA levels in DN and non-DN patients can help the follow-up and management for the progression of endothelial dysfunction. The purpose of this research was to know the difference of ADMA levels in DN and non-DN by a cross-sectional observational analytical method in 53 diabetes mellitus patients at the Dr. Hasan Sadikin Hospital Bandung (December 2016-July 2017). Urine samples were examined to calculate urinary creatinine albumin ratio (uACR) and serum for ADMA levels. Asymetric dimethylarginin was examined by micro ELISA. Most of the subjects were males (60.38%) with the highest age in the range of 55-64 years (45.28%). Increased ADMA levels were found in 100% of DN and 18.5% of non-DN. Median ADMA levels were found in DN 1.01(0.73-2.25) µmol/L and non-DN 0.57(0.27-1.17) µmol/L, showing a significant difference of ADMA levels (p<0.001). High ADMA levels showed endothelial dysfunction in DN. Serum ADMA levels in DN patients were higher than in non-DN.

PERBANDINGAN KADAR HEMOGLOBIN ANTARA METODE SPECTROPHOTOMETER DENGAN METODE HEMOCUE PADA SAMPEL LEUKOSITOSIS Basti Andriyoko; Leni Lismayanti; Delita Prihatni
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.975

Background and objective: The measurement of haemoglobin was carried out by using hematology analyzer with specthrophotometermethod which measured light absorbance at 540 nm. However, this measurement affected by increase turbidity as a result of leucocytosis.The turbidity can be eliminated by using HemoCue method that detect absorbance at 570 nm and 880 nm. The aim of this study wasto compare the measurement of haemoglobin obtained between specthrophotometer method and HemoCue method. Materials andmethod: Blood EDTA sample that have been measured with MEK-6318K Nihon Kohden hematology analyzer for haemoglobin levelswith spectrophotometer methods with leucocyte > 100.000/mm3 were included in this study. Blood sample are measured again forthe haemoglobin level with HemoCue B-Haemoglobin Analyzer. This study was conducted at Clinical Pathology Laboratory, Dr. HasanSadikin Hospital Bandung from August–October 2008. Result: Seventeen leucocytosis sample were enroled in this study. The meanhaemoglobin level from specthrophotometer method is higher than HemoCue method, however there was no statistically significantdifference between haemoglobin result from specthrophotometer method and HemoCue method (p = 0.742 > a = 0.05). Conclusion:There was no significant difference beetween specthrophotometer method and HemoCue method for haemoglobin measurement ofleucocytosis sample.

Snake-Bite with Disseminated Intravascular Coagulation (DIC) and Stage II Hypertension Hendra Subroto; Leni Lismayanti
Journal of Medicine and Health Vol. 1 No. 5 (2017)
Publisher : Universitas Kristen Maranatha

Snake-bite is an important medical emergency case and caused of many hospitaladmission especially in the rural area, forests, plantations and swamps. Despite its importance,there have been fewer proper data of snake-bite incidence in Indonesia. World HealthOrganization estimate that at least 421,000 envenomings and 20,000 deaths from snakebitesoccur each year, especially in South and South East Asia and sub-Saharan Africa. The authorsreport a case of a 76-year-old man came to Hasan Sadikin Hospital with chief complaint woundin his right hand and right forearm from snake-bite. Snake-bites can cause DIC because thevenom activates the coagulation system and cause fibrinolysis which occurs in less than 24hours. Laboratory results, we found abnormalities such as anemia, thrombocytopenia,hypofibrinogenemia, and increased levels of D-dimer. Patients were treated for 8 days and thenallowed to go home. Snake-bite is an occupational disease of farmers, plantation workers,herdsmen, fishermen, other. Snake bite cases require prompt and comprehensive managementso as to minimize the possibility of disability and death.Keywords: snake bite, DIC, hypertension

Positive Lateral Flow Urine Lipoarabinomannan Assay (LF-LAM) Result in Detection of Active Tuberculosis Sara Puspita; Dewi Kartika Turbawaty; Nina Tristina; Leni Lismayanti
Majalah Kedokteran Bandung Vol 53, No 3 (2021)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v53n3.2265

Lipoarabinomannan (LAM) is the main component of M. tuberculosis (MTB) wall as result of MTB degradation by macrophages in the human body. In patients with active TB and HIV co-infection, a decrease in antibody responses may be apparent that some of LAM may not be bound with antibodies. In this condition, LAM can pass through the normal glomerular basement membrane and can be detected in the urine. One laboratory examination for detecting LAM is the Lateral Flow Urine Lipoarabinomannan (LF-LAM) assay that uses urine as the sample. The purpose of this cross-sectional observational descriptive comparative study was to compare the positivity rate of LF-LAM examination results in active TB patients with and without HIV infection. Random urine samples were collected from patients diagnosed with active TB with and without HIV infection who visited Dr. Hasan Sadikin General Hospital Bandung from August to October 2020. The proportion between the group with HIV and group without HIV was analyzed with the Chi-Square test. Subjects were 52 patients, consisting of 25 (48%) subjects with HIV infection and 27 (52%) subjects without HIV infection. The positive LF-LAM results were found in 11 (21%) subjects, consisting of 9 (36%) subjects with HIV infection and 2 (7%) subjects without HIV infection, with p=0.012. In conclusion, the positivity rate of LF-LAM results is higher in active TB patients with HIV infection compared to those without HIV infection.

Comparison of Reticulocyte Hemoglobin Equivalent Levels between Low and Normal Birth Weight Newborns Resvi Livia; Fajar Wasilah; Leni Lismayanti
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 29, No 1 (2022)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v29i1.1943

Low Birth Weight (LBW) newborns face a risk of iron deficiency. Iron deficiency hinders growth, and motoric, and cognitive development. Newborns with LBW sometimes suffer from inflammation, which affects the commonly used iron measurements. Reticulocyte hemoglobin equivalent (Ret-He) is considered a potential tool to measure iron profile because it measures functional iron, and it is not affected by inflammation. This study compared the Ret-He in LBW and normal birth weight newborns. This cross-sectional study was done retrospectively by observing and comparing the hematology data of newborns from November to December 2019. The difference in Ret-He level was assessed using a non-parametric test. Out of 70 newborns, 26 were normal and 44 were LBW. The proportion of LBW newborns with anemia was higher than the proportion of normal ones (29.6% vs 7.7%, p=0.03). The median value of Ret-He in LBW was lower compared to normal birth weight (32.6 vs 33.3 pg, p=0.09), however, the values were still within the normal limits. Five from 70 of these newborns' Ret-He levels were under the reference range (7.14%). There was found that CRP levels were higher in LBW newborns than normal ones (5.6% vs 5%, p=0.98). There was a positive correlation between Ret-He and the birth weight of the newborns (r= 0.34, p =<0.01). There was no significant difference in Ret-He levels of LBW compared to normal babies. Further research is needed with a larger sample size to better assess the association of Ret-He and iron profiles in newborns.

Analysis of C-Peptide Levels Among Gynaecological Malignancies Patients Underwent Chemotherapy with Carboplatin Regiment Nina Tristina; Juandika Juandika; Leni Lismayanti; Adhi Kristianto Sugianli; Raja Iqbal Mulya Harahap
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 29, No 1 (2022)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v29i1.1967

C-peptide is part of the, which its amounts were equal to endogenous insulin secreted by pancreatic β cells. Carboplatin is one of the chemotherapy regimens that are widely used to treat gynecological malignancies. Carboplatin may cause the damage of β-islets of Langerhans, which may cause defects in insulin synthesis leading to secondary diabetes mellitus or other types of diabetes mellitus. The purpose of this study was to determine the differences in C-peptide, (which reflects endogenous insulin levels) levels in patients with gynecologic malignancy who underwent carboplatin chemotherapy. This study was a comparative observational study with a cross-sectional design. There was a total of 42 subjects who met the inclusion criteria. Subjects with gynecological malignancy post-carboplatin chemotherapy regimens had lower serum C-peptide levels in group II compared to group I. Serum C-peptide levels can further be used to monitor side effects of carboplatin and can be used as a test to diagnose the other types of diabetes mellitus especially before starting the fourth cycle

Comparison of Reticulocyte Hemoglobin Equivalent Levels between Low and Normal Birth Weight Newborns Resvi Livia; Fajar Wasilah; Leni Lismayanti
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 29 No. 1 (2022)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v29i1.1943

Low Birth Weight (LBW) newborns face a risk of iron deficiency. Iron deficiency hinders growth, and motoric, and cognitive development. Newborns with LBW sometimes suffer from inflammation, which affects the commonly used iron measurements. Reticulocyte hemoglobin equivalent (Ret-He) is considered a potential tool to measure iron profile because it measures functional iron, and it is not affected by inflammation. This study compared the Ret-He in LBW and normal birth weight newborns. This cross-sectional study was done retrospectively by observing and comparing the hematology data of newborns from November to December 2019. The difference in Ret-He level was assessed using a non-parametric test. Out of 70 newborns, 26 were normal and 44 were LBW. The proportion of LBW newborns with anemia was higher than the proportion of normal ones (29.6% vs 7.7%, p=0.03). The median value of Ret-He in LBW was lower compared to normal birth weight (32.6 vs 33.3 pg, p=0.09), however, the values were still within the normal limits. Five from 70 of these newborns' Ret-He levels were under the reference range (7.14%). There was found that CRP levels were higher in LBW newborns than normal ones (5.6% vs 5%, p=0.98). There was a positive correlation between Ret-He and the birth weight of the newborns (r= 0.34, p =<0.01). There was no significant difference in Ret-He levels of LBW compared to normal babies. Further research is needed with a larger sample size to better assess the association of Ret-He and iron profiles in newborns.

Analysis of C-Peptide Levels Among Gynaecological Malignancies Patients Underwent Chemotherapy with Carboplatin Regiment Nina Tristina; Juandika Juandika; Leni Lismayanti; Adhi Kristianto Sugianli; Raja Iqbal Mulya Harahap
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 29 No. 1 (2022)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v29i1.1967

C-peptide is part of the, which its amounts were equal to endogenous insulin secreted by pancreatic Î² cells. Carboplatin is one of the chemotherapy regimens that are widely used to treat gynecological malignancies. Carboplatin may cause the damage of Î²-islets of Langerhans, which may cause defects in insulin synthesis leading to secondary diabetes mellitus or other types of diabetes mellitus. The purpose of this study was to determine the differences in C-peptide, (which reflects endogenous insulin levels) levels in patients with gynecologic malignancy who underwent carboplatin chemotherapy. This study was a comparative observational study with a cross-sectional design. There was a total of 42 subjects who met the inclusion criteria. Subjects with gynecological malignancy post-carboplatin chemotherapy regimens had lower serum C-peptide levels in group II compared to group I. Serum C-peptide levels can further be used to monitor side effects of carboplatin and can be used as a test to diagnose the other types of diabetes mellitus especially before starting the fourth cycle

Thawing Time Difference between Fresh Frozen Plasma Using Ziplock Plastic and non-Ziplock Plastic in Blood Transfusion Unit Dr. Hasan Sadikin General Hospital Bandung, Indonesia Rinanty, Rima; Lismayanti, Leni; Harahap, Raja Iqbal Mulya
Althea Medical Journal Vol 10, No 3 (2023)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v10n3.2795

Background: The quality of fresh frozen plasma (FFP) in a clinical setting depends on thawing time. Thawing using a water bath is often used in blood transfusion units because it is easy to perform, affordable, and easy to look for. Protective plastics (Ziplock and non-Ziplock) are used to reduce the risk of contamination,. This study aimed to determine whether there is a difference in thawing time between FFP using Ziplock plastic and non-Ziplock plastic.Method: This experimental design was conducted in the Blood Bank Unit of Hasan Sadikin Hospital from June-August 2021. Samples were divided into two groups, namely thawing using Ziplock and non-Ziplock. Each group consisted of volume 160-200 ml and 201-240 ml.Results: The total samples were 24 FFP bags. In the group of the bag 160-200 ml, the median thawing time using Ziplock plastic was 8 minutes (8-16 minutes), non-Ziplock was 15 minutes (8-16 minutes) (p value 0,111), whereas in a group of bags with volume 201-240 ml, the median thawing time using Ziplock was 15 minutes (8-28 minutes), non-Ziplock was 20 minutes (14-30 minutes) (p- value 0,332). Although there was a time difference in both groups, the difference was non-significant.Conclusion: The thawing time between the small bag with a volume of 160-200 ml and the larger volume of 201-240 ml shows no difference. Ziplock plastic can be used to reduce the risk of contamination.

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