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All Journal Biospecies Edukasi: Jurnal Pendidikan Jurnal Pendidikan Bahasa JURNAL PENDIDIKAN TAMBUSAI Bio-Lectura Jurnal Agroecotania : Publikasi Nasional Ilmu Budidaya Pertanian GERVASI: Jurnal Pengabdian kepada Masyarakat SeBaSa: Jurnal Pendidikan Bahasa dan Sastra Indonesia Cakrawala Linguista Ksatra: Jurnal Kajian Bahasa dan Sastra Khazanah Intelektual Journal of Language Education, Linguistics, and Culture Jurnal Karya Abdi Masyarakat Jurnal Ilmiah Ilmu Terapan Universitas Jambi Jurnal Pelayanan dan Pengabdian Masyarakat Indonesia SEGANTANG LADA : JURNAL PENGABDIAN KESEHATAN Deiksis: Jurnal Pendidikan Bahasa dan Sastra Indonesia
Lizawati Lizawati
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Journal : Biospecies

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Induksi kalus eksplan daun Anggrek Pensil (Papillionanthe hookeriana Rchb.f.) pada Kombinasi Zat Pengatur Tumbuh 2,4-D dan BAP Neliyati, Neliyati; Lizawati, Lizawati
Biospecies Vol. 17 No. 1 (2024): Januari 2024
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v17i1.31492

Abstract

The formation of callus is one of the indications of success in propagation through tissue culture. A crucial factor in callus induction is the accuracy in determining the type and concentration of auxin and cytokinin growth regulators in the culture media. This research aims to obtain the appropriate concentration of auxin (2,4-D) and cytokinin (BAP) growth regulators that can induce the formation of embryonic callus propagules from pencil orchid plants using tissue culture propagation techniques. The treatments tested in this study were the application of several concentrations of auxin (2,4-D) at 0.5; 1.0; 1.5, and 2.0 ppm combined with cytokinin (BAP) at concentrations of 0.0 and 0.5 ppm. The experiment was arranged in a Completely Randomized Design (CRD) with 15 explants for each treatment. The parameters observed were the response time of the explant (swelling), the percentage of explants swelling, the appearance of callus, the percentage of explants forming callus, callus structure, and callus color. The results showed that the pencil orchid leaf explants responded by swelling and forming callus. Swelling occurred fastest two weeks after culturing. The highest percentage of swelling and callus formation was obtained from the treatment of MS media supplemented with a combination of 2,4-D 2.0 ppm + 0.5 ppm cytokinin BAP, with 100% of the explants swelling and 26,67% forming callus. The formed callus was friable in structure and white in color.
OPTIMALISASI ZAT PENGATUR TUMBUH 2,4-D DAN BAP PADA PROLIFERASI KALUS KOPI LIBERIKA (COFFEA LIBERICA) SECARA IN VITRO. Hidayat, Rahmat; Lizawati, Lizawati; Rainiyati, Rainiyati; Zulkarnain, Zulkarnain; Eliyanti , Eliyanti; Kartika, Elis
Biospecies Vol. 18 No. 2 (2025): Juli 2025
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v18i2.44922

Abstract

Coffee is one of the strategic plantation commodities that has high economic value and an important role in the national economy. Among the various types of coffee cultivated in Indonesia, Coffea liberica has great development potential, especially in areas such as Jambi Province. However, conventional propagation of Liberica coffee plants through seeds orcuttings still faces obstacles, such as low success rates and slow growth rates. Therefore, tissue culture techniques are an alternative solution to produce Liberika coffee seedlings in bulk, quickly, and uniformly. This study aims to optimize the effect of growth regulator (ZPT) concentration interaction of auxin (2,4-D) and cytokinin (BAP) on liberica coffee callusformation in vitro. Experiments were conducted at the Plant Biotechnology Laboratory, Jambi University, using a factorial completely randomized design (CRD) with two factors. The first factor is the concentration of 2,4-D which consists of two levels, namely 1 ppm (d1) and 2 ppm (d2). The second factor is the concentration of BAP which consists of five levels, namely 1 ppm (b1), 2 ppm (b2), 3 ppm (b3), 4 ppm (b4), and 5 ppm (b5). Parameters observed included color, structure, size, and callus weight after 12 weeks of culture. The results showed that the interaction between 2,4-D and BAP significantly affected all observation parameters. Callus with the highest size (2.66 cm) and weight (2.90 g) were obtained in the combination of 1 ppm 2,4-D + 4 ppm BAP and 2 ppm 2,4-D + 1 ppm BAP respectively. The callus was yellowishwhite to green and had a crumbly or compact structure, depending on the ZPT combination. In conclusion, a balanced combination of ZPT concentrations greatly determines the success of Liberika coffee callus proliferation, and the results of this study provide an essential basis for the development of efficient coffee tissue culture.
Respons Tanaman Jarak Pagar Terhadap Mikoriza Indigenous dan Pupuk P di Lahan Bekas Tambang Batu Bara KARTIKA, Elis; LIZAWATI, LIZAWATI; HAMZAH, Hamzah
Biospecies Vol. 11 No. 1 (2018): January 2018
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.037 KB) | DOI: 10.22437/biospecies.v11i1.4993

Abstract

The objective of this research was to assess the response of Jatropha curcas to indigenous Arbuscular Mycorrhizal Fungi (AMF) and P fertilizers applications in former coal mine fields. This study used a single factor Randomized Block Design which is a combination of mycorrhiza and P fertilizer consisting of 10 levels (without FMA and without P fertilizer, without FMA and 25% recommended doses of P fertilizer, without FMA and 50% recommended doses of P fertilizer, without FMA and 75% recommended doses of P fertilizer, without FMA and 100% recommended doses of P fertilizer, FMA and without P fertilizer, FMA and 25% recommended doses of P fertilizer, FMA and 50% recommended doses of P fertilizer, FMA and 75% doses of P fertilizer, FMA and 100% recommended doses of P fertilizer), with recommended doses is 150 Kg SP 36 / ha The observed variables were N, P, K uptake and Jatropha curcas yield. The results showed that the symbiotic of Jatropha curcas with FMA combined with 50% recommended dose of P fertilizer gave the best N, P, K uptake and Jatropha curcas yield in Coal Post-Mining Land.
Co-Authors Agus Kurniawan Mastur Agustin, Ria Alivia, Widya Asriwati, Asriwati Dedy Antony Effran, Endy Elis Kartika Eliyanti , Eliyanti Elva Sulastriana Elva Sulastriana, Elva Eti Ramaniyar, Eti Fadhilah, Umu Fitri Wulansari FITRY TAFZI Fuad Muchlis Gusniwati , Hamzah Hamzah Herlina Herlina Jumiati Jumiati Khaulani, Dzarin Gifarian Liestyaningrum, Wiwiek Miranti Sari Fitriani Muhammad Lahir Neliyati, Neliyati Netti Yuniarti Nuraini Nuraini Pujiati , Wasis Rahmat Hidayat Rainiyati Rainiyati Ratno Ratno Rini Agustina Riska Aprillia Winingsih Saedah, Saedah Saptiana Sulastri, Saptiana Sina, Cian Ibnu Sri Kusnita Sri Kusnita, Sri Sulastriana, Eva Try Hariadi Utari Utari Weni Wilia Yulia Alia Zulkarnain Zulkarnain