Article Per Year (5 Year)
p-Index From 2021 - 2026
2.57
P-Index
This Author published in this journals
All Journal Jurnal Biosains Pascasarjana Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Majalah Obstetri dan Ginekologi Jurnal Kreativitas PKM Manuju : Malahayati Nursing Journal Faletehan Health Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Layanan Masyarakat (Journal of Public Service) CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL Jurnal Widya Medika
Puspa Wardhani
Departemen Patologi Klinik Fakultas Kedokteran Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

Published : 29 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 29 Documents
Search

 1   2   3 
AST, ALT and Albumin Level in Chronic Hepatitis B Patients with and without Complications of Cirrhosis and Hepatocellular Carcinoma Viky Nafi'ah Rahma Maulidia; Puspa Wardhani; Bagus Setyoboedi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 26 No. 3 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i3.1588

Abstract

Complications of Chronic Hepatitis B (CHB) infection are liver cirrhosis and Hepatocellular Carcinoma (HCC). Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), and albumin may be used as indicators of hepatocyte damage. This study aimed to determine differences between AST, ALT, and albumin in CHB patients without complications with CHB patients with cirrhosis and HCC complications. An analytical cross-sectional study was conducted in March-May 2019 on 62 CHB patients with or without cirrhosis or HCC complications using the total sampling method. AST and ALT were calculated using the Siemens Dimension device and IFCC method. The data were analyzed using independent samples T-test Albumin in CHB patients without complications was higher than CHB patients who had complications of liver cirrhosis (p=0.002). The AST and ALT were not significantly different. Aspartate aminotransferase in CHB with cirrhosis complications differed from CHB patients who had HCC complications (p=0.015), however, not different in ALT and albumin. Aspartate aminotransferase, ALT, and albumin in CHB patients without complications were different from those with HCC complications. Albumin in CHB patients without complications was different from CHB patients with cirrhosis complications. Aspartate aminotransferase in CHB patients with cirrhosis complications was different from CHB patients who had HCC complications. As a result of these differences, an integrated approach to intervening liver damage may be needed to prevent the progression of the disease from becoming more severe.
Genetic Diversity of Plasmodium falciparum Glutamate Rich Protein in Patients Attending the Merauke Hospital in Papua Province, Indonesia Thomas Tandi Manu; Puspa Wardhani; Heny Arwati; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1662

Abstract

Malaria remains an important health problem in Indonesia with the highest transmission in Papua Province, an eastern part of this country. The genetic diversity of malaria parasites is the main problem in understanding several aspects of malaria infections and the dynamics of their transmission, which also play a role in the development of a vaccine. Plasmodium falciparum is the deadliest of the human malaria parasites. Plasmodium falciparum glutamate-rich protein (Pfglurp) is one of the many erythrocytic stages antigens currently under development for a vaccine. The Pfglurp gene hasbeen extensively used as a marker to investigate the genetic diversity, Multiplicity of Infection (MOI), the level of malaria transmission, immunity against malaria, as well as a discriminatory instrument to distinguish new from recrudescent infections of the field parasite population. Thus, this genotyping study aimed to find out the genetic population of P.falciparum at the Merauke District, Province of Papua, Indonesia. DNA samples were isolated from Dried Blood Spots (DBS) obtained from P.falciparum infected patients in the Regional Public Hospital of Merauke, Province of Papua, Indonesiaduring May 2019-July 2019. The isolated DNAs were then amplified for nested Polymerase Chain Reaction (PCR) prior to Pfglurp genotyping. The glurp gene was identified in all 51 DBS samples of P.falciparum-infected patients, and 18 variants of allele were found. Among them, 45.10% were found to bear multigenotype infections. The size of the dominant allele (12.5%) was 701-750 bp. The MOI was 1.58. The genetic population of P.falciparum in Merauke Hospital has contained a higher percentage of multigenotypes compared with monogenotypes indicating the high transmission of malaria in the studied area.
Correlation between APRI, FIB-4 and GPR Indices to Fibroscan and HBeAg Status in Patients with Chronic Hepatitis B Rina Erlina; Puspa Wardhani; Yessy Puspitasari; Ulfa Kholili
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 3 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i3.1718

Abstract

Liver fibrosis is a complication of chronic hepatitis B. Early detection of liver fibrosis is important for therapy. The aspartate aminotransferase index (AST)-to-platelet ratio index (APRI) and the fibrosis index based on 4 factors (FIB-4) in chronic hepatitis B have been widely studied despite the inconsistent results. Research on other serum markers is extensively carried out, including Gamma-Glutamyl Transpeptidase (GGT)-to-platelet ratio (GPR). Previous studies have shown that the GPR index was more accurate than APRI and FIB-4. HBeAg status is an indication for therapy. There have not been many studies on the correlation of serum markers with HBeAg status. This study aimed to determine the correlation of APRI, FIB-4, and GPR with Fibroscan and HBeAg status in chronic hepatitis B patients. A cross-sectional study was carried out from June to September 2020 and found 50 chronic hepatitis B patients. Platelet count was measured using a Sysmex XN-1000 hematology device; AST, alanine aminotransferase (ALT), and GGT levels were measured using the Dimension RXL clinical chemistry device; and the degree of fibrosis was determined using transient elastography (Fibroscan). Spearman correlation test was used in this study for the correlation analysis. The results showed a significant correlation between APRI, FIB-4 and GPR indices with Fibroscan (r=0.454, p 0.001; r=0.610, p < 0.001; r=0.540, p < 0.001, respectively). A significant correlation was found between APRI, FIB-4 and GPR indices with negative (-) HBeAg (r=0.486, p 0.004; r=0.648, p < 0.001; r=0.595, p < 0.001, respectively). In addition, a significant correlation was found between FIB-4 and positive (+) HBeAg (r=0.499, p 0.049), but no correlation was found between APRI and GPR with positive (+) HBeAg (r=0.295, p 0,267; r=0.386, p 0.140, respectively).
Validity of Chemiluminescent Immunoassay Serology Test for Anti-Sars Cov-2 Antibodies IgM and IgG 1 Museyaroh Museyaroh; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1725

Abstract

In December 2019, an outbreak of acute pneumonia occurred in Wuhan, China. The disease was transmitted between humans through droplets (coughing or sneezing) of infected patients, causing this outbreak to spread rapidly in various countries in the world, including Indonesia. On February 11, 2020, WHO announced the pneumonia was caused by Coronavirus Disease 2019 (COVID-19), which was caused by a new type of Coronavirus, the SARS-CoV-2. A rapid and accurate diagnosis is critical for the control of the COVID-19 outbreak. The widely used test is a serology-based test that detects the  presence of SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of the methods used for this test is Chemiluminescent Immunoassay (CLIA). This study aimed to determine the reliability of CLIA. The study was conducted from August to September 2020. The number of samples was 63 patients' serum. Polymerase chain reaction examination at Husada Utama Hospital, Surabaya, revealed that 21 patients were confirmed positive for COVID-19 with positive PCR results, and 42 patients were healthy with negative COVID-19 results. The results showed that IgM had a diagnostic sensitivity of 85.7%, diagnostic specificity of 92.8%, a positive predictive value of 85.7%, a negative predictive value of 92.8%,and accuracy of 90.4%. In comparison, IgG had a diagnostic sensitivity of 90.4%, diagnostic specificity of 90.4%, a positive predictive value of 82.6%, a negative predictive value of 90.5%, and accuracy of 90.4%. In conclusion, IgG has a higher sensitivity than IgM, while IgM had higher specificity, positive predictive value, and negative predictive value than IgG. However, the positive, negative predictive value and efficiency values were the same for IgM and IgG.
Profile of Tumor Necrosis Factor Alpha Levels in Childhood Malignancy with Febrile Neutropenia Tigor Pandapotan Sianturi; Puspa Wardhani; I Dewa Gede Ugrasena
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 3 (2022)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i3.1886

Abstract

Infection is a significant cause of morbidity and mortality in childhood malignancy with Febrile Neutropenia (FN). Tumor Necrosis Factor-Alpha (TNF-α) is involved in host defense against bacterial invasion. However, changes in TNF-α levels with the possibility of bacterial infection confirmed by blood culture are still unclear. The study aimed to evaluate TNF-α levels in childhood malignancy with FN who had blood cultures with a control group. Observational cross-sectional analysis during January-October 2020 at Dr. Soetomo General Academic Hospital, Surabaya. Childhood malignancy with FN episodes as the case group and nonfebrile neutropenia as the control. TNF-α levels examination used plasma with the Enzyme-Linked Immunosorbent Assay (ELISA) sandwich method. Blood culture results were obtained from the patient's medical record. The differences in TNF-α levels in the case groups and control were analyzed by the T-square test for two independent samples or Mann-Whitney U according to the data distribution. There were 18 cases group with 30 FN episodes and 15 controls. There were 8(26.66%) positive and 22(73.33%) negative blood cultures from 30 FN episodes. The mean TNF-α levels in the positive blood culture cases group and control: 14.72±5.77 and 9.78±2.74 pg/mL, and the median (min-max) negative blood cultures: 12.19 (7.01-25.70) pg/mL. There was no significant difference in TNF-α levels in the positive and negative blood culture cases group (p=0.527), but there was a significant difference in the control (p=0.049 and p=0.027). Therefore, TNF-α levels cannot be used as a marker of bacterial infection in the case groups.
Correlation Glucose, Uric Acid, and Cholesterol Levels Towards Health Conditions in the Highlands: POCT Approach Arifa Mustika; Yetti Hernaningsih; Puspa Wardhani; Siti Khaerunnisa; Nastasya Nunki; Baiq Nasha Islaeli; Uli Mas'uliyah Indarwati; Musholli Himmatun Nabilah; Teguh Satrio; Alfino Validita Sidiq; Fita Triastuti; Mochammad Reza Desianto; Agil Saputra; Novi Ersanto; Widaninggar Rahma Putri; Desty Indah Sari
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 30 No. 3 (2024)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v30i3.2203

Abstract

Degenerative diseases arise when age increases due to the weakening of the body's physiological condition. The purpose of this study was to measure glucose, uric acid, and cholesterol levels in residents of Segunung Village to improve lifestyles and quality of life. As many as 110 residents of Segunung Village were used as subjects in this cross-sectional study. Glucose, uric acid, and cholesterol levels were measured using Point of Care Testing (POCT). A cut-off value of <200 was used to define high cholesterol levels. Only cholesterol levels showed an increase when compared with reference values, while glucose and uric acid levels were normal. Interestingly glucose was significantly correlated with age (r=0.309, p=0.001). The approach using POCT describes the overall condition of Segunung villagers and can be used as a basis for glucose, uric acid, and cholesterol screening tests.
The Effect Between Transverse Abdominis Plane Block And Quadratus Lumborum Block On Endorphin Beta Levels And Pain Scales In Post Cesarean Section Patients Rudyanto Wiharjo Seger; Christrijogo Sumartono; Puspa Wardhani; Aditiawarman Aditiawarman
JURNAL WIDYA MEDIKA Vol. 6 No. 2 (2020): October
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v6i2.2783

Abstract

Background: Cesarean section is a surgical procedure that is often performed in labor and causes moderate to severe pain for 48 hours postoperatively. The provision of local anesthesia drugs post-cesarean section can be given by the Transverse Abdominis Plane (TAP) block and Quadratus Lumborum (QL) block. This study was to determine the effect of analgesia between the Transverse Abdominis Plane block and Quadratus Lumborum block on the scale of post-cesarean section pain and level of the beta-endorphin post-operative cesarean section under spinal anesthesia. Methods: An experimental randomized controlled clinical trial study was conducted on 30 pregnant women aged 18-45 years post-cesarean section who were treated electively with ASA I - II and anesthesia under spinal anesthesia. Patients who met the inclusion criteria were treated by dividing three groups randomly through the computer. In group A, control and Transverse Abdominis Plane Block are given; group B, given control and Quadratus Lumborum Block; and group C, given control (ketorolac and tramadol). Furthermore, it was recorded and measured the level of beta-endorphin, and the Wong Baker Faces Scale (WBFS) pain scale postoperatively and 6 hours postoperatively. Data collected then analyzed by SPSS’s computer program. Results: The effect of QL block administration helps relieve the WBFS pain scale by five times and decreases beta-endorphin level by 0.2 times compared to TAP block administration. The effect of QL block administration helped relieve the WBFS pain scale by 13.5 times and decreased beta-endorphin level by 5.4 times compared to standard therapy. The effect of TAP block administration helps to reduce the WBFS pain scale by 7.4 times and to reduce beta-endorphin level by 5.1 times compared to standard therapy. Conclusion: QL block relieves the WBFS pain scale and decreases the beta-endorphin level better than the TAP block. Keywords: Cesarean section, post-operative pain, beta-endorphin, Quadratus Lumborum block, Transversus Abdominis Plane block
Penguatan Manajemen Laboratorium dalam Menurunkan Morbiditas dan Mortalitas Hepatitis B Aditea Etawati Putri; Yetti Hernaningsih; Puspa Wardhani; Diah Puspita Rini; Yulia Nadar Indrasari; Nanda Aulya Ramadhan
Faletehan Health Journal Vol 12 No 01 (2025): Faletehan Health Journal, Maret 2025
Publisher : Universitas Faletehan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33746/fhj.v12i01.765

Abstract

Hepatitis B virus (HBV) infection is one of the threats in the medical world and requires guaranteed and valid laboratory analysis procedures, one of which is through the quality control (QC) mechanism. As part of laboratory management, QC has an impact on the validity of laboratory analysis results, especially related to HBV infection. The purpose of this study was to determine the level of knowledge of medical analysts before and after laboratory management training. The research method used comparative paired test analysis to obtain the significance and binary logistic regression to determine the most influential questions on the test. The results showed a significant increase in medical analyst knowledge after being given laboratory management material. Supported by a p value = 1.000 from the Hosmer and Lemeshow test and an overall prediction of 95.5% (positive: 92.0% and negative 97.6%), this training was very good in terms of goodness-of-fit and accurate. Material related to immunochromatography (ICT) method is an influential factor based on the analysis of test results. Medical analyst training, especially related to laboratory management in the context of HBV management, has a significant impact and can be continued and developed.
Laboratory Parameter Profile in Hepatocellular Carcinoma Patients admitted to the wards of Internal Medicine at an Indonesian Tertiary Hospital Namira Amelia Septiarini; Puspa Wardhani; Yulia Nadar Indrasari; Ummi Maimunah
Current Internal Medicine Research and Practice Surabaya Journal Vol. 7 No. 1 (2026): CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cimrj.v7i1.66464

Abstract

Background: Hepatocellular Carcinoma (HCC) is a liver cancer caused by abnormal cell changes, potentially fatal, and is the eighth most common cancer in the world. Diagnosis can be done through liver function tests, tumor markers, and imaging examinations. Thus study aimed to determine the profile of laboratory parameters in HCC patients at Dr. Soetomo Hospital Surabaya in 2020-2022. Methods: The method used was a retrospective study of 67 patient medical records with secondary data collection through total sampling techniques. Laboratory tests performed included SGOT, SGPT, Albumin, Direct Bilirubin, Total Bilirubin, PT, aPTT, and AFP. Results: This study showed that the most common age group was 55-59 years, with the majority of patients being male (70.14%) from East Java (91%). The main complaints were abdominal discomfort and abdominal enlargement (61.19%), with the highest causative factor being Hepatitis B (44.77%). Most patients were at BCLC stage D (17.18%). Laboratory parameters showed median and minimum maximum values for SGOT were 135 (20.76–1436), SGPT 59 (17–671), albumin level 2.96 ± 0.62, direct bilirubin 1 (0.02–22.96), total bilirubin 2.05 (0.09–30.84), PT 14.7 (10.1–47.7), and aPTT 30.2 (20.7–85.5). A total of 16 patients had AFP levels above 400ng/mL. The results of USG and CT Scan examinations showed multiple tumors in most patients. Conclusion: The profile of HCC patients in the inpatient room internal medicine Dr. Soetomo Hospital Surabaya in 2020-2022 was dominated by male patients aged 55-59 years, the main complaint was abdominal discomfort, the most common risk factors were Hepatitis B, BCLC stage D, abnormal liver function, total AFP levels above 400 ng/mL, and USG and CT-Scan examinations showed multiple tumors.
Co-Authors Aditea Etawati Putri Aditiawarman Aditiawarman, Aditiawarman Agil Saputra Ahmad Yudianto Alfino Validita Sidiq Arifa Mustika Arifa Mustika Aryati Aryati Aryati Aryati Ashon Sa’adi Audia Mumtaz Rifasky Bagus Setyoboedi Baiq Nasha Islaeli Budi Utomo Christrijogo Soemartono Waloejo Darsini, Ninik Desty Indah Sari Diah Puspita Rini Dwiyanti Puspitasari, Dwiyanti Erawati Erawati Faizah, Zakiyatul Fauqa Arinil Aulia Fita Triastuti Furqoni, Abdul Hadi Hamdani Lunardhi Harsasi Setyawati Hebert Adrianto Heny Arwati I Dewa Gede Ugrasena I.G.A.A Putri Sri Rejeki Ilham Harlan Amarullah Laily Nur Azizah Maesarah Maesarah Martono Martono Mochammad Reza Desianto Museyaroh, Museyaroh Musholli Himmatun Nabilah Namira Amelia Septiarini Nanda Aulya Ramadhan Nastasya Nunki Novi Ersanto Nyilo Purnami Puspitasari, Yessy Rina Erlina Risa Etika, Risa Rizqidhana Juliana Putri Rudyanto Wiharjo Seger Shifa Fauziyah Shuhai Ueda Siti Churrotin, Siti Siti Khaerunnisa Soegeng Soegijanto Sri Subekti Suci Tresna Teguh Hari Sucipto, Teguh Hari Teguh Satrio Thomas Tandi Manu Tigor Pandapotan Sianturi Trieva Verawaty Butarbutar Ulfa Kholili Uli Mas'uliyah Indarwati Ummi Maimunah Viky Nafi&#039;ah Rahma Maulidia Viky Nafi'ah Rahma Maulidia Widaninggar Rahma Putri Yetti Hernaningsih Yovilianda Maulitiva Untoro Yulia Nadar Indrasari