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All Journal Jurnal Biosains Pascasarjana Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Majalah Obstetri dan Ginekologi Jurnal Kreativitas PKM Manuju : Malahayati Nursing Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Layanan Masyarakat (Journal of Public Service)
Puspa Wardhani
Departemen Patologi Klinik, Fakultas Kedokteran, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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AST, ALT and Albumin Level in Chronic Hepatitis B Patients with and without Complications of Cirrhosis and Hepatocellular Carcinoma Viky Nafi'ah Rahma Maulidia; Puspa Wardhani; Bagus Setyoboedi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 26 No. 3 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i3.1588

Abstract

Complications of Chronic Hepatitis B (CHB) infection are liver cirrhosis and Hepatocellular Carcinoma (HCC). Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), and albumin may be used as indicators of hepatocyte damage. This study aimed to determine differences between AST, ALT, and albumin in CHB patients without complications with CHB patients with cirrhosis and HCC complications. An analytical cross-sectional study was conducted in March-May 2019 on 62 CHB patients with or without cirrhosis or HCC complications using the total sampling method. AST and ALT were calculated using the Siemens Dimension device and IFCC method. The data were analyzed using independent samples T-test Albumin in CHB patients without complications was higher than CHB patients who had complications of liver cirrhosis (p=0.002). The AST and ALT were not significantly different. Aspartate aminotransferase in CHB with cirrhosis complications differed from CHB patients who had HCC complications (p=0.015), however, not different in ALT and albumin. Aspartate aminotransferase, ALT, and albumin in CHB patients without complications were different from those with HCC complications. Albumin in CHB patients without complications was different from CHB patients with cirrhosis complications. Aspartate aminotransferase in CHB patients with cirrhosis complications was different from CHB patients who had HCC complications. As a result of these differences, an integrated approach to intervening liver damage may be needed to prevent the progression of the disease from becoming more severe.
Genetic Diversity of Plasmodium falciparum Glutamate Rich Protein in Patients Attending the Merauke Hospital in Papua Province, Indonesia Thomas Tandi Manu; Puspa Wardhani; Heny Arwati; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1662

Abstract

Malaria remains an important health problem in Indonesia with the highest transmission in Papua Province, an eastern part of this country. The genetic diversity of malaria parasites is the main problem in understanding several aspects of malaria infections and the dynamics of their transmission, which also play a role in the development of a vaccine. Plasmodium falciparum is the deadliest of the human malaria parasites. Plasmodium falciparum glutamate-rich protein (Pfglurp) is one of the many erythrocytic stages antigens currently under development for a vaccine. The Pfglurp gene hasbeen extensively used as a marker to investigate the genetic diversity, Multiplicity of Infection (MOI), the level of malaria transmission, immunity against malaria, as well as a discriminatory instrument to distinguish new from recrudescent infections of the field parasite population. Thus, this genotyping study aimed to find out the genetic population of P.falciparum at the Merauke District, Province of Papua, Indonesia. DNA samples were isolated from Dried Blood Spots (DBS) obtained from P.falciparum infected patients in the Regional Public Hospital of Merauke, Province of Papua, Indonesiaduring May 2019-July 2019. The isolated DNAs were then amplified for nested Polymerase Chain Reaction (PCR) prior to Pfglurp genotyping. The glurp gene was identified in all 51 DBS samples of P.falciparum-infected patients, and 18 variants of allele were found. Among them, 45.10% were found to bear multigenotype infections. The size of the dominant allele (12.5%) was 701-750 bp. The MOI was 1.58. The genetic population of P.falciparum in Merauke Hospital has contained a higher percentage of multigenotypes compared with monogenotypes indicating the high transmission of malaria in the studied area.
Correlation between APRI, FIB-4 and GPR Indices to Fibroscan and HBeAg Status in Patients with Chronic Hepatitis B Rina Erlina; Puspa Wardhani; Yessy Puspitasari; Ulfa Kholili
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 3 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i3.1718

Abstract

Liver fibrosis is a complication of chronic hepatitis B. Early detection of liver fibrosis is important for therapy. The aspartate aminotransferase index (AST)-to-platelet ratio index (APRI) and the fibrosis index based on 4 factors (FIB-4) in chronic hepatitis B have been widely studied despite the inconsistent results. Research on other serum markers is extensively carried out, including Gamma-Glutamyl Transpeptidase (GGT)-to-platelet ratio (GPR). Previous studies have shown that the GPR index was more accurate than APRI and FIB-4. HBeAg status is an indication for therapy. There have not been many studies on the correlation of serum markers with HBeAg status. This study aimed to determine the correlation of APRI, FIB-4, and GPR with Fibroscan and HBeAg status in chronic hepatitis B patients. A cross-sectional study was carried out from June to September 2020 and found 50 chronic hepatitis B patients. Platelet count was measured using a Sysmex XN-1000 hematology device; AST, alanine aminotransferase (ALT), and GGT levels were measured using the Dimension RXL clinical chemistry device; and the degree of fibrosis was determined using transient elastography (Fibroscan). Spearman correlation test was used in this study for the correlation analysis. The results showed a significant correlation between APRI, FIB-4 and GPR indices with Fibroscan (r=0.454, p 0.001; r=0.610, p < 0.001; r=0.540, p < 0.001, respectively). A significant correlation was found between APRI, FIB-4 and GPR indices with negative (-) HBeAg (r=0.486, p 0.004; r=0.648, p < 0.001; r=0.595, p < 0.001, respectively). In addition, a significant correlation was found between FIB-4 and positive (+) HBeAg (r=0.499, p 0.049), but no correlation was found between APRI and GPR with positive (+) HBeAg (r=0.295, p 0,267; r=0.386, p 0.140, respectively).
Validity of Chemiluminescent Immunoassay Serology Test for Anti-Sars Cov-2 Antibodies IgM and IgG 1 Museyaroh Museyaroh; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1725

Abstract

In December 2019, an outbreak of acute pneumonia occurred in Wuhan, China. The disease was transmitted between humans through droplets (coughing or sneezing) of infected patients, causing this outbreak to spread rapidly in various countries in the world, including Indonesia. On February 11, 2020, WHO announced the pneumonia was caused by Coronavirus Disease 2019 (COVID-19), which was caused by a new type of Coronavirus, the SARS-CoV-2. A rapid and accurate diagnosis is critical for the control of the COVID-19 outbreak. The widely used test is a serology-based test that detects the  presence of SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of the methods used for this test is Chemiluminescent Immunoassay (CLIA). This study aimed to determine the reliability of CLIA. The study was conducted from August to September 2020. The number of samples was 63 patients' serum. Polymerase chain reaction examination at Husada Utama Hospital, Surabaya, revealed that 21 patients were confirmed positive for COVID-19 with positive PCR results, and 42 patients were healthy with negative COVID-19 results. The results showed that IgM had a diagnostic sensitivity of 85.7%, diagnostic specificity of 92.8%, a positive predictive value of 85.7%, a negative predictive value of 92.8%,and accuracy of 90.4%. In comparison, IgG had a diagnostic sensitivity of 90.4%, diagnostic specificity of 90.4%, a positive predictive value of 82.6%, a negative predictive value of 90.5%, and accuracy of 90.4%. In conclusion, IgG has a higher sensitivity than IgM, while IgM had higher specificity, positive predictive value, and negative predictive value than IgG. However, the positive, negative predictive value and efficiency values were the same for IgM and IgG.
Profile of Tumor Necrosis Factor Alpha Levels in Childhood Malignancy with Febrile Neutropenia Tigor Pandapotan Sianturi; Puspa Wardhani; I Dewa Gede Ugrasena
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 3 (2022)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i3.1886

Abstract

Infection is a significant cause of morbidity and mortality in childhood malignancy with Febrile Neutropenia (FN). Tumor Necrosis Factor-Alpha (TNF-α) is involved in host defense against bacterial invasion. However, changes in TNF-α levels with the possibility of bacterial infection confirmed by blood culture are still unclear. The study aimed to evaluate TNF-α levels in childhood malignancy with FN who had blood cultures with a control group. Observational cross-sectional analysis during January-October 2020 at Dr. Soetomo General Academic Hospital, Surabaya. Childhood malignancy with FN episodes as the case group and nonfebrile neutropenia as the control. TNF-α levels examination used plasma with the Enzyme-Linked Immunosorbent Assay (ELISA) sandwich method. Blood culture results were obtained from the patient's medical record. The differences in TNF-α levels in the case groups and control were analyzed by the T-square test for two independent samples or Mann-Whitney U according to the data distribution. There were 18 cases group with 30 FN episodes and 15 controls. There were 8(26.66%) positive and 22(73.33%) negative blood cultures from 30 FN episodes. The mean TNF-α levels in the positive blood culture cases group and control: 14.72±5.77 and 9.78±2.74 pg/mL, and the median (min-max) negative blood cultures: 12.19 (7.01-25.70) pg/mL. There was no significant difference in TNF-α levels in the positive and negative blood culture cases group (p=0.527), but there was a significant difference in the control (p=0.049 and p=0.027). Therefore, TNF-α levels cannot be used as a marker of bacterial infection in the case groups.
Correlation Glucose, Uric Acid, and Cholesterol Levels Towards Health Conditions in the Highlands: POCT Approach Arifa Mustika; Yetti Hernaningsih; Puspa Wardhani; Siti Khaerunnisa; Nastasya Nunki; Baiq Nasha Islaeli; Uli Mas'uliyah Indarwati; Musholli Himmatun Nabilah; Teguh Satrio; Alfino Validita Sidiq; Fita Triastuti; Mochammad Reza Desianto; Agil Saputra; Novi Ersanto; Widaninggar Rahma Putri; Desty Indah Sari
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 30 No. 3 (2024)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v30i3.2203

Abstract

Degenerative diseases arise when age increases due to the weakening of the body's physiological condition. The purpose of this study was to measure glucose, uric acid, and cholesterol levels in residents of Segunung Village to improve lifestyles and quality of life. As many as 110 residents of Segunung Village were used as subjects in this cross-sectional study. Glucose, uric acid, and cholesterol levels were measured using Point of Care Testing (POCT). A cut-off value of <200 was used to define high cholesterol levels. Only cholesterol levels showed an increase when compared with reference values, while glucose and uric acid levels were normal. Interestingly glucose was significantly correlated with age (r=0.309, p=0.001). The approach using POCT describes the overall condition of Segunung villagers and can be used as a basis for glucose, uric acid, and cholesterol screening tests.
Co-Authors Agil Saputra Ahmad Yudianto Alfino Validita Sidiq Arifa Mustika Arifa Mustika Aryati Aryati Aryati Aryati Ashon Sa’adi Audia Mumtaz Rifasky Bagus Setyoboedi Baiq Nasha Islaeli Budi Utomo Desty Indah Sari Dwiyanti Puspitasari, Dwiyanti Erawati Erawati Faizah, Zakiyatul Fauqa Arinil Aulia Fita Triastuti Furqoni, Abdul Hadi Hamdani Lunardhi Harsasi Setyawati Hebert Adrianto Heny Arwati I Dewa Gede Ugrasena I.G.A.A Putri Sri Rejeki Ilham Harlan Amarullah Laily Nur Azizah Maesarah Maesarah Martono Martono Mochammad Reza Desianto Museyaroh, Museyaroh Musholli Himmatun Nabilah Nastasya Nunki Ninik Darsini Novi Ersanto Nyilo Purnami Puspitasari, Yessy Rina Erlina Risa Etika, Risa Rizqidhana Juliana Putri Shifa Fauziyah Shuhai Ueda Siti Churrotin, Siti Siti Khaerunnisa Soegeng Soegijanto Sri Subekti Suci Tresna Teguh Hari Sucipto, Teguh Hari Teguh Satrio Thomas Tandi Manu Tigor Pandapotan Sianturi Trieva Verawaty Butarbutar Ulfa Kholili Uli Mas'uliyah Indarwati Viky Nafi&#039;ah Rahma Maulidia Viky Nafi'ah Rahma Maulidia Widaninggar Rahma Putri Yetti Hernaningsih Yovilianda Maulitiva Untoro Yulia Nadar Indrasari