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All Journal Jurnal Biosains Pascasarjana Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Majalah Obstetri dan Ginekologi Jurnal Kreativitas PKM Manuju : Malahayati Nursing Journal Care : Jurnal Ilmiah Ilmu Kesehatan JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Layanan Masyarakat (Journal of Public Service)
Puspa Wardhani
Departemen Patologi Klinik, Fakultas Kedokteran, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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AST, ALT and Albumin Level in Chronic Hepatitis B Patients with and without Complications of Cirrhosis and Hepatocellular Carcinoma Viky Nafi'ah Rahma Maulidia; Puspa Wardhani; Bagus Setyoboedi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 3 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i3.1588

Abstract

Complications of Chronic Hepatitis B (CHB) infection are liver cirrhosis and Hepatocellular Carcinoma (HCC). Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), and albumin may be used as indicators of hepatocyte damage. This study aimed to determine differences between AST, ALT, and albumin in CHB patients without complications with CHB patients with cirrhosis and HCC complications. An analytical cross-sectional study was conducted in March-May 2019 on 62 CHB patients with or without cirrhosis or HCC complications using the total sampling method. AST and ALT were calculated using the Siemens Dimension device and IFCC method. The data were analyzed using independent samples T-test Albumin in CHB patients without complications was higher than CHB patients who had complications of liver cirrhosis (p=0.002). The AST and ALT were not significantly different. Aspartate aminotransferase in CHB with cirrhosis complications differed from CHB patients who had HCC complications (p=0.015), however, not different in ALT and albumin. Aspartate aminotransferase, ALT, and albumin in CHB patients without complications were different from those with HCC complications. Albumin in CHB patients without complications was different from CHB patients with cirrhosis complications. Aspartate aminotransferase in CHB patients with cirrhosis complications was different from CHB patients who had HCC complications. As a result of these differences, an integrated approach to intervening liver damage may be needed to prevent the progression of the disease from becoming more severe.
Seropositivity of Anti-Rubella Antibodies as A Marker for Rubella Infection in Infants at High Risk of Congenital Deafness Nyilo Purnami; Risa Etika; Martono Martono; Puspa Wardhani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1479

Abstract

Hearing loss in newborns or congenital deafness can be caused by the development of several parts of the auditorysystem. Congenital deafness is often associated with infections, such as Toxoplasmosis, Rubella, Cytomegalovirus (CMV),and Herpes (TORCH). Deafness is very difficult to be early detected. Therefore, simple but fast methods are needed. Earlydetection is based on the Newborn Hearing Screening (NHS) program. Otoacoustic Emission (OAE) and AutomatedAuditory Brainstem Response (AABR) checks are raw materials for early detection. Congenital deafness often occurs withpregnancy infections with viruses such as Rubella. Rubella infection during pregnancy, especially during the first trimester,often causes Congenital Rubella Syndrome (CRS). Rubella infection often occurs with other causes, such as Toxoplasma,CMV, and Herpes. A Serological test can be used as one of the diagnostics of this infection. This study used single RubellaIgG and IgM antibodies and double antibodies test as a marker for the infection. The authors wanted to correlate theserological examination of this infection with the auditory function. Rubella infection was detected with single serologicalanti-Rubella IgG and IgM and double multiple Rubella and TORCH serological tests. Also, the auditory function wasassessed using the OAE and AABR test in this research. The result showed 35 (77.7%) patients with positive Rubellaserological tests among 45 NICU patients at Dr. Soetomo Hospital. There were number of patients was 12 (34.2%) patientswith a single positive serological test and 23 (65.7%) patients with positive multiple TORCH serological tests. The number ofpatients with Rubella negative infection was 10 (22.2%). There were 11 (31.4%) patients of positive Rubella infections withpositive hearing loss and 24 (68.6%) patients with negative hearing loss. From the results of the study, 35 patients were athigh risk of disturbance and the statistical analysis showed that there were no significant serological differences in Rubellapositive with hearing loss (p=0.087). Hearing loss in NICU infants has a high risk of factors causing Rubella infection andother related causes. In most Rubella positive serological tests IgG was found, which can be due to maternal factors.Serology tests need to be repeated for confirmation under the surveillance program. How to follow-up the patients anddefine the next laboratory test after six months remain a great challenge. The efforts need to be strengthened in surveillanceprograms.
Validity of Chemiluminescent Immunoassay Serology Test for Anti-Sars Cov-2 Antibodies IgM and IgG 1 Museyaroh Museyaroh; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 28, No 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1725

Abstract

In December 2019, an outbreak of acute pneumonia occurred in Wuhan, China. The disease was transmitted betweenhumans through droplets (coughing or sneezing) of infected patients, causing this outbreak to spread rapidly in variouscountries in the world, including Indonesia. On February 11, 2020, WHO announced the pneumonia was caused byCoronavirus Disease 2019 (COVID-19), which was caused by a new type of Coronavirus, the SARS-CoV-2. A rapid andaccurate diagnosis is critical for the control of the COVID-19 outbreak. The widely used test is a serology-based test thatdetects the presence of SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of the methods used for this test isChemiluminescent Immunoassay (CLIA). This study aimed to determine the reliability of CLIA. The study was conductedfrom August to September 2020. The number of samples was 63 patients' serum. Polymerase chain reaction examination atHusada Utama Hospital, Surabaya, revealed that 21 patients were confirmed positive for COVID-19 with positive PCRresults, and 42 patients were healthy with negative COVID-19 results. The results showed that IgM had a diagnosticsensitivity of 85.7%, diagnostic specificity of 92.8%, a positive predictive value of 85.7%, a negative predictive value of 92.8%,and accuracy of 90.4%. In comparison, IgG had a diagnostic sensitivity of 90.4%, diagnostic specificity of 90.4%, a positivepredictive value of 82.6%, a negative predictive value of 90.5%, and accuracy of 90.4%. In conclusion, IgG has a highersensitivity than IgM, while IgM had higher specificity, positive predictive value, and negative predictive value than IgG.However, the positive, negative predictive value and efficiency values were the same for IgM and IgG.
Profile of Tumor Necrosis Factor Alpha Levels in Childhood Malignancy with Febrile Neutropenia Tigor Pandapotan Sianturi; Puspa Wardhani; I Dewa Gede Ugrasena
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 28, No 3 (2022)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i3.1886

Abstract

Infection is a significant cause of morbidity and mortality in childhood malignancy with Febrile Neutropenia (FN). Tumor Necrosis Factor-Alpha (TNF-α) is involved in host defense against bacterial invasion. However, changes in TNF-α levels with the possibility of bacterial infection confirmed by blood culture are still unclear. The study aimed to evaluate TNF-α levels in childhood malignancy with FN who had blood cultures with a control group. Observational cross-sectional analysis during January-October 2020 at Dr. Soetomo General Academic Hospital, Surabaya. Childhood malignancy with FN episodes as the case group and nonfebrile neutropenia as the control. TNF-α levels examination used plasma with the Enzyme-Linked Immunosorbent Assay (ELISA) sandwich method. Blood culture results were obtained from the patient's medical record. The differences in TNF-α levels in the case groups and control were analyzed by the T-square test for two independent samples or Mann-Whitney U according to the data distribution. There were 18 cases group with 30 FN episodes and 15 controls. There were 8(26.66%) positive and 22(73.33%) negative blood cultures from 30 FN episodes. The mean TNF-α levels in the positive blood culture cases group and control: 14.72±5.77 and 9.78±2.74 pg/mL, and the median (min-max) negative blood cultures: 12.19 (7.01-25.70) pg/mL. There was no significant difference in TNF-α levels in the positive and negative blood culture cases group (p=0.527), but there was a significant difference in the control (p=0.049 and p=0.027). Therefore, TNF-α levels cannot be used as a marker of bacterial infection in the case groups.
PELATIHAN KADER KESEHATAN DAN GELAR POTENSI ALAM DALAM PROGRAM PAKETDATA (PANDUAN KESEHATAN DESA WISATA) KAMPUNG ADAT SEGUNUNG, WONOSALAM, JOMBANG Arifa Mustika; Yetti Hernaningsih; Puspa Wardhani
Jurnal Layanan Masyarakat Vol. 6 No. 2 (2022): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v6i2.2022.355-362

Abstract

Desa wisata merupakan kawasan yang mempunyai kearifan lokal yang berpotensi mempunyai daya tarik wisata. Meningkatnya peminat wisata desa dan melimpahnya desa wisata di Indonesia harus diimbangi dengan sumber daya manusia (SDM) yang memadai dalam mengelola dan mengembangkannya, terutama dalam hal kesehatan. Tujuan pengabdian masyarakat ini untuk memberikan edukasi dan pemahaman pada SDM dalam bentuk pelatihan dan penyuluhan kesehatan khususnya di desa wisata, desa kampung adat Segunung Wonosalam Jombang Jawa Timur. Metode yang diaplikasikan dalam pelaksanaan pengabdian masyarakat di desa wisata kampung adat Segunung ini ada dua langkah, yaitu penyuluhan dan pelatihan, dilaksanakan selama 3 hari. Hari pertama pelatihan dengan tema first aid, peserta diberikan pre-test dan post-test sebelum dan sesudah pelatihan. Hari kedua Penyuluhan kesehatan (tema: pengetahuan tentang tanaman obat, pengolahan bahan pangan, dan pola hidup sehat), peserta pada penyuluhan kesehatan diberikan pre-test dan post-test sebelum dan sesudah penyuluhan. Hari ketiga penyuluhan tentang NAPZA. Hasil pre-test dan post-test pada pelatihan first aid didapatkan peningkatan persentase jumlah peserta yang mendapatkan nilai ≥ 60 sebesar 56% dan pada penyuluhan kesehatan didapatkan peningkatan 47%. Penyuluhan dan pelatihan ini diharapkan dapat dipraktikkan dikemudian hari dan bermanfaat untuk kelangsungan objek wisata kampung adat Segunung.
Profil Pemeriksaan Darah Lengkap pada Pasien Anak Infeksi Virus Dengue di Instalasi Rawat Inap Smf Anak Rsud Dr. Soetomo Surabaya Maesarah Maesarah; Puspa Wardhani; Dwiyanti Puspitasari
Malahayati Nursing Journal Vol 5, No 1 (2023): Volume 5 Nomor 1 2023
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v5i1.7679

Abstract

ABSTRACT In Indonesia, dengue virus infection has a high mortality rate. Hemoglobin, hematocrit, leukocyte, differential count, and thrombocyte are laboratory parameters frequently used to monitor patient progress. To study the pattern of hemoglobin, hematocrit, leukocyte, differential count, and thrombocyte based on defervescence in pediatric patient with viral dengue infections. This was a descriptive observational study using medical records at General Hospital Dr. Soetomo Surabaya from February to August 2012. The total sample were 77 pediatric patients in the aged range of 1-18 years old with DF or DHF and according to inclusion and exclusion criteria. The average hemoglobin and hematocrit levels of DHF were higher than DF, both of increased in D0. The average leukocyte count of DF was lower than DHF, both of decreased on D-0. The average lymphocyte count of DHF decreased on D-3, conversely DF continued to increase. The average monocyte count of DHF increased in D-1, then decreased with DF to D+4. The average basophil count of DHF increased in D-0, while DF increased in D+2. The average eosinophil count of DHF increased in D-3. The average neutrophil count of DHF continued to increase in D-3, but was lower in D0, while DD decreased in D+3. The average thrombocyte count of DHF and DD were the lowest on D+3 and D+1. The average hemoglobin and hematocrit levels of DF and DHF increased on D0. The average thrombocyte count of DHF was lower than DF especially on D-1 to D+1. The average leukocyte count of DF and DHF decreased on D-1. The average monocyte, basophil, and eosinophil count of DHF were higher than DF on D0. While the average monocyte and neutrophil count of DHF were lower than DF on D0. Keyword: Dengue, Pediatric Patients, Defervescence , Thrombocyte, Leukocyte, Hemoglobin, Hematocrit  ABSTRAK Infeksi virus dengue di Indonesia masih memiliki angka kematian cukup tinggi. Hemoglobin, hematokrit, leukosit, hitung jenis leukosit, dan trombosit sering digunakan untuk memantau perkembangan pasien. Mengetahui pola hemoglobin, hematokrit, leukosit, hitung jenis leukosit, dan trombosit berdasarkan waktu defervescence pada pasien anak infeksi virus dengue. Penelitian deskriptif observasional menggunakan data rekam medik pasien di Instalasi Rawat Inap SMF Anak RSUD Dr. Soetomo Surabaya antara Februari-Agustus tahun 2012. Total sampel adalah 77 pasien anak usia 1-18 tahun dengan DD atau DBD serta memenuhi kriteria inklusi dan eksklusi. Rerata kadar hemoglobin dan hematokrit DBD lebih tinggi dibandingkan DD, keduanya meningkat pada H0. Jumlah leukosit DD lebih rendah dibandingkan DBD, keduanya menurun pada H-1. Rerata jumlah limfosit DBD menurun pada H-3, sebaliknya DD terus meningkat. Rerata jumlah monosit DBD meningkat pada H+1 kemudian menurun dengan DD sampai H+4. Rerata jumlah basofil DBD meningkat pada H0, sebaliknya DD meningkat pada H+2. Rerata jumlah eosinofil DBD meningkat sejak awal demam. Rerata jumlah neutrofil DBD terus meningkat namun lebih rendah pada H0, sedangkan DD menurun pada H+3. Rerata jumlah trombosit DBD dan DD terendah pada H+1 dan H-1. Rerata kadar hemoglobin dan hematokrit DD dan DBD meningkat pada H0. Rerata jumlah trombosit DBD lebih rendah dibandingkan DD terutama pada H-1 sampai H+1. Rerata jumlah leukosit DD dan DBD menurun pada H-1. Rerata jumlah monosit, basofil, dan eosinofil DBD lebih tinggi dibandingkan DD pada H0. sebaliknya jumlah monosit dan neutrofil DBD lebih rendah dibandingkan DD pada H0. Kata Kunci: Dengue, Anak, Defervescence, Trombosit, Leukosit, Hemoglobin, Hematokrit 
Peningkatan Pengetahuan tentang Pemeriksaan Spesimen Laboratorium COVID-19 melalui Edukasi Pencegahan dan Pengendalian Infeksi Yulia Nadar Indrasari; Fauqa Arinil Aulia; Yessy Puspitasari; Puspa Wardhani; Yetti Hernaningsih
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 6, No 6 (2023): Volume 6 No 6 Juni 2023
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v6i6.9783

Abstract

ABSTRAK Wabah COVID-19 (coronavirus disease 2019) yang pertama kali berasal dari Wuhan, Cina telah menyebar ke 219 negara dan telah menginfeksi sekitar 104.911.243 penduduk di seluruh dunia. Sejak kasus ini pertama kali dilaporkan pada tanggal 2 Maret 2020 di Indonesia, jumlah kasus terkonfirmasi terus meningkat dan penularan penyakit menyebar dengan cepat di seluruh wilayah tanah air. Hal ini turut berdampak pada keselamatan dan kesehatan tenaga kesehatan yang bertugas dalam penanganan COVID-19. Faktor paparan virus, tekanan kerja yang terlalu berat, penggunaan APD yang tidak adekuat, hingga kelelahan fisik dan mental menjadi pemicu utama krisis kesehatan di lingkungan tenaga kesehatan. Kegiatan pengabdian masyarakat ini diharapkan dapat mengendalikan jumlah tenaga kesehatan yang tertular COVID-19 saat melakukan pelayanan kesehatan kepada pasien. Kegiatan pengabdian masyarakat yang dilaksanakan di ruang pertemuan Hotel Ningrat, Kabupaten Bangkalan berupa edukasi dan penyuluhan tentang pencegahan dan pengendalian infeksi (PPI) di dalam pemeriksaan spesimen COVID-19. Sebanyak 100 peserta tenaga kesehatan puskesmas Dinas Kesehatan Kabupaten Bangkalan mempraktekkan Gerakan Cuci Tangan (hand hygiene) sesuai standar WHO, serta pemasangan dan pelepasan alat pelindung diri (APD) yang benar. Nilai post-test para partisipan petugas kesehatan puskesmas baik dokter umum, perawat, bidan, maupun ATLM yang telah mendapatkan pengetahuan, informasi, dan ketrampilan pencegahan dan pengendalian infeksi (PPI) sebesar 78 peserta (86,7%) dari 100 partisipan mengalami peningkatan dibandingkan nilai pre-test sebelum pemberian materi edukasi. Kegiatan ini dapat meningkatkan pemahaman mengenai penyakit COVID-19 dan cara penularannya, yang dapat diamati pada peningkatan nilai postes peserta penyuluhan. Kegiatan edukasi dan penyuluhan tentang PPI perlu dilakukan berkesinambungan agar dapat menurunkan jumlah tenaga Kesehatan yang tertular COVID-19. Kata Kunci: COVID-19, Pencegahan dan Pengendalian Infeksi.  ABSTRACT The COVID-19 (coronavirus disease 2019) outbreak, which first originated in Wuhan, China, has spread to 219 countries and has infected around 104,911,243 people worldwide. Since this case was first reported on March 2, 2020, in Indonesia, the number of confirmed cases has continued to increase, and disease transmission has spread rapidly throughout the country. This also has an impact on the safety and health of health workers on duty in handling COVID-19. Factors such as exposure to viruses, excessive work pressure, inadequate use of PPE, and physical and mental exhaustion are the main triggers for the health crisis in the health workforce. This activity is expected to control the number of health workers who are infected with COVID-19 when providing health services to patients. Community service activities carried out in the Ningrat Hotel meeting room, Bangkalan Regency in the form of education and counseling about infection prevention and control in examining COVID-19 specimens. A total of 100 health workers at the Bangkalan District Health Office practiced hand hygiene according to WHO standards and the correct installation and removal of personal protective equipment (PPE) The post-test scores of participants in health center health workers, including general practitioners, nurses, midwives, and ATLM who had received knowledge, information, and infection prevention and control (PPI) skills, were 78 participants (86.7%) out of 100 participants, an increase compared to pre-test values before giving educational materials. This activity can increase understanding of the COVID-19 disease and its mode of transmission, which can be observed in the increase in the posttest value of the counseling participants. Education and outreach activities about PPI must be carried out continuously to reduce the number of health workers infected with COVID-19. Keywords: COVID-19, Infection Prevention and Control.
Description of Fecal Culture Results in Diarrhea Patients Due To Antibiotic Use Suci Tresna; I.G.A.A Putri Sri Rejeki; Puspa Wardhani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 26 No. 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1448

Abstract

Diarrhea infection is common in developing countries and causes death of around 3 million people every year. Diarrhea is also the second leading cause of death in infants. Riskesdas in 2013 showed 30,775 cases of diarrhea. Causes were such as bacterial infections Salmonella, Shigella, Vibrio, Entamoeba, and Yersinia. Other influences can occur due to viral and fungal infections. Diarrhea is a nosocomial infection that is common in hospitalized patients due to the long-term use of antibiotics caused by Clostridium difficile. This study was a follow-up study of diarrhea patients who received antibiotic therapy for more than two days with the results of C.difficile negative toxin, then continued with fecal culture examination. This study aimed to look at the description causes of diarrhea other than C.difficile in patients who received long-term antibiotic therapy. This research is an observational study. Samples were taken from 30 diarrhea patients with 2 x 24 hours of antibiotic use who were hospitalized in the ICU, Dr. Soetomo Hospital Surabaya from August 2017 to May 2018. Samples with negative C.difficiletoxin results were then followed by fecal culture examination using conventional methods. The results of culture examination from 30 samples showed three samples with positive culture results extended-spectrum β lactamase producing E.coli, two samples positive culture just E.coli, and 25 other samples showed negative culture results. The results of the fecal culture examination showed a description of causes of diarrhea in patients who received antibiotic therapy was pathogenic E.coli (ESBL). The possibility of other causes that cannot be detected from the culture such as viral and fungal infections, still requires further research.  
Seropositivity of Anti-Rubella Antibodies as A Marker for Rubella Infection in Infants at High Risk of Congenital Deafness Nyilo Purnami; Risa Etika; Martono Martono; Puspa Wardhani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 26 No. 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1479

Abstract

Hearing loss in newborns or congenital deafness can be caused by the development of several parts of the auditory system. Congenital deafness is often associated with infections, such as Toxoplasmosis, Rubella, Cytomegalovirus (CMV), and Herpes (TORCH). Deafness is very difficult to be early detected. Therefore, simple but fast methods are needed. Early detection is based on the Newborn Hearing Screening (NHS) program. Otoacoustic Emission (OAE) and Automated Auditory Brainstem Response (AABR) checks are raw materials for early detection. Congenital deafness often occurs with pregnancy infections with viruses such as Rubella. Rubella infection during pregnancy, especially during the first trimester, often causes Congenital Rubella Syndrome (CRS). Rubella infection often occurs with other causes, such as Toxoplasma, CMV, and Herpes. A Serological test can be used as one of the diagnostics of this infection. This study used single Rubella IgG and IgM antibodies and double antibodies test as a marker for the infection. The authors wanted to correlate the serological examination of this infection with the auditory function. Rubella infection was detected with single serological anti-Rubella IgG and IgM and double multiple Rubella and TORCH serological tests. Also, the auditory function was assessed using the OAE and AABR test in this research. The result showed 35 (77.7%) patients with positive Rubella serological tests among 45 NICU patients at Dr. Soetomo Hospital. There were number of patients was 12 (34.2%) patients with a single positive serological test and 23 (65.7%) patients with positive multiple TORCH serological tests. The number of patients with Rubella negative infection was 10 (22.2%). There were 11 (31.4%) patients of positive Rubella infections with positive hearing loss and 24 (68.6%) patients with negative hearing loss. From the results of the study, 35 patients were at high risk of disturbance and the statistical analysis showed that there were no significant serological differences in Rubella positive with hearing loss (p=0.087). Hearing loss in NICU infants has a high risk of factors causing Rubella infection and other related causes. In most Rubella positive serological tests IgG was found, which can be due to maternal factors. Serology tests need to be repeated for confirmation under the surveillance program. How to follow-up the patients and define the next laboratory test after six months remain a great challenge. The efforts need to be strengthened in surveillance programs.
Diagnostic Value of Plasmotec Malaria-3 Antigen Detection on Gold Standard Microscopy Trieva Verawaty Butarbutar; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 26 No. 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1529

Abstract

Plasmotec Malaria-3 is a rapid malaria diagnostic test that uses four-line tests and targets three malaria proteins, namely Plasmodium falciparum specific protein (HRP-2), Plasmodium vivax-specific LDH (Pv-LDH) and non-specific Plasmodium LDH (pLDH). Microscopy as a gold standard has many disadvantages and the availability of malaria Rapid Diagnostic Tests (RDTs) in detecting three proteins is still very limited. This study aimed to determine the diagnostic value of ® ® Plasmotec Malaria-3 against gold standard microscopy, comparing the Plasmotec Malaria-3 and microscopy antigen ® species detection, determining the Parasitemia Index (PI) cut-off using Plasmotec Malaria-3. This study was a cross-sectional study with 105 whole blood samples obtained from the Merauke Papua General Hospital which fulfilled the inclusion and exclusion criteria. Samples were examined by thick and thin drops and then examined with Plasmotec® ® Malaria-3. Diagnostic values of Plasmotec Malaria-3 against the microscopy were Sn 100%, Sp 98.04%, PPV 98.18%, NPV ® 100%, LR + 51, LR-0, diagnostic accuracy of 99.05%. Comparison of Plasmodium species between Plasmotec Malaria-3 and ® microscopy was not significantly different, p-value = 0.172. The cut-off of PI in P.falciparum and P.vivax in Plasmotec Malaria-3 based on the Receiver Operating Characteristic (ROC) curve could not be determined with AUC=0.577, p-value=0.385 and AUC=0.423, p-value=0.385, respectively. This study concluded that the comparison of Plasmodium ® species between Plasmotec Malaria-3, and microscopy was not significantly different. This study suggested that further ® research is needed to find the diagnostic value of non-falciparum and non-vivax Plasmodium against Plasmotec Malaria-3.
Co-Authors Agil Saputra Ahmad Yudianto Alfino Validita Sidiq Anandia Nafisah Putri Arifa Mustika Arifa Mustika Aryati Aryati Aryati Aryati Ashon Sa’adi Audia Mumtaz Rifasky Bagus Setyoboedi Baiq Nasha Islaeli Budi Utomo Desty Indah Sari Dwiyanti Puspitasari, Dwiyanti Erawati Erawati Faizah, Zakiyatul Fauqa Arinil Aulia Fita Triastuti Furqoni, Abdul Hadi Hamdani Lunardhi Harsasi Setyawati Hebert Adrianto Heny Arwati Hermina Novida, Hermina I Dewa Gede Ugrasena I.G.A.A Putri Sri Rejeki Ilham Harlan Amarullah Laily Nur Azizah Maesarah Maesarah Martono Martono Mochammad Reza Desianto Museyaroh, Museyaroh Musholli Himmatun Nabilah Nastasya Nunki Ninik Darsini Novi Ersanto Nyilo Purnami Puspitasari, Yessy Rina Erlina Risa Etika, Risa Rizqidhana Juliana Putri Shifa Fauziyah Shuhai Ueda Siti Churrotin, Siti Siti Khaerunnisa Soegeng Soegijanto Sri Subekti Suci Tresna Teguh Hari Sucipto Teguh Hari Sucipto, Teguh Hari Teguh Satrio Thomas Tandi Manu Tigor Pandapotan Sianturi Trieva Verawaty Butarbutar Ulfa Kholili Uli Mas'uliyah Indarwati Viky Nafi'ah Rahma Maulidia Viky Nafi'ah Rahma Maulidia Widaninggar Rahma Putri Yetti Hernaningsih Yovilianda Maulitiva Untoro Yulia Nadar Indrasari