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All Journal Agrifor : Jurnal Ilmu Pertanian dan Kehutanan CHEMPUBLISH JOURNAL Science and Technology Indonesia As-syifaa Jurnal Farmasi Jurnal Kesehatan Medika Saintika Jurnal Katalisator PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JURNAL KESEHATAN PERINTIS Baselang: Jurnal Ilmu Pertanian, Peternakan, Perikanan dan Lingkungan Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) JAFP (Jurnal Akademi Farmasi Prayoga) Jurnal Katalisator Journal of Pharmaceutical and Sciences. FASKES : Jurnal Farmasi, Kesehatan, dan Sains Jurnal Riset Multidisiplin dan Inovasi Teknologi Rafflesia Journal of Natural and Applied Sciences (RJNAS) DIVERSITAS HAYATI: Journal of Conservation and Biological Resources
Wardi, Epi Supri
Fakultas Farmasi Universitas Perintis Indonesia Padang, Sumatera Barat, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Desain Primer dan Deteksi Gen CHS (Chalcone Synthase) pada Tanaman Gambir (Uncaria gambir (Hunter) Roxb.) Tipe Riau Mancik Nova, Bastian; Wardi, Epi Supri; Rahmi, Miftahur; Zikri, Fadillatul
Baselang Vol 4, No 1: APRIL 2024
Publisher : Fakultas Pertanian Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/bsl.v4i1.124

Abstract

Gambir merupakan salah satu tanaman yang menghasilkan katekin. Kandungan katekin merupakan parameter dalam menentukan mutu gambir. Salah satu gen yang terlibat dalam pembentukkan katekin adalah gen CHS (Chalcone synthase). Penelitian ini bertujuan untuk mendapatkanprimer yang dapat digunakan untuk mendeteksi gen CHS (Chalcone synthase) pada tanaman gambir tipe Riau Mancik serta untuk melihat binding site dengan hasil isolasi DNA tanaman gambir terhadap primer yang telah didesain. Pendesainan primer dilakukan dengan mengalignment 21 data tanaman yang mengandung sekuen gen CHS yang terdapat di NCBI. Hasil desain primer didapatkan 8 pasang primer degeneratif dengan 4 primer forward dan 2 primer reverse. Primer yang memiliki binding site dengan hasil isolasi DNA yaitu pasangan primer A1F dengan C1R menghasilkan produk dengan estimasi 724 bp.
Metabolit Bioaktif Bakteri: Tinjauan tentang Potensi Antimikroba dan Aplikasinya Nova, Bastian; Supri Wardi, Epi; Sartika, Diza
Journal of Pharmaceutical and Sciences JPS Volume 7 Nomor 3 (2024)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v7i3.514

Abstract

Bacteria are a promising source of bioactive compounds. This review provides a concise overview of existing research on antimicrobial molecules from bacteria. Additionally, it briefly summarizes bacteriocins, non-ribosomal peptides, polyketides, and lipopeptides targeting pathogens resistant to several drugs, considering next-generation antibiotics. The review highlights the potential use of bacteria as a source of antimicrobials for biotechnological, nutraceutical, and pharmaceutical applications. However, further investigation is needed to isolate, separate, purify, and characterize these bioactive compounds, as well as to formulate them into clinically approved antibiotics.
Barkod DNA Tumbuhan Pegagan (Centella asiatica (L) Urban) Berdasarkan Gen rbcL Dan matK Nova, Bastian; Wardi, Epi Supri; Irwandi, Irwandi; Sari, Atika Aulia
Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Vol 25, No 1 (2026): Maret 2026
Publisher : Universitas 17 Agustus 1945 Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31293/agrifor.v25i1.7569

Abstract

Pegagan (Centella asiatica (L.) Urban) adalah tanaman yang banyak digunakan dalam pengobatan tradisional untuk menyembuhkan berbagai penyakit. Identifikasi tanaman pegagan diperlukan untuk memastikan spesiesnya. Salah satu metode identifikasi tanaman adalah DNA barcoding. Primer yang umum digunakan dalam DNA barcoding tanaman adalah primer rbcL dan matK. Penelitian ini dilakukan untuk mengetahui primer DNA barcoding terbaik yang digunakan dalam mengidentifikasi pegagan hijau, merah, embun, dan kapsul pegagan menggunakan gen matK dan rbcL. Isolasi DNA dilakukan dengan menggunakan Thermo Scientific GeneJet Plant Genomic DNA Purification Mini Kit. Berdasarkan pencarian pada sistem BOLD, primer matK untuk pegagan hijau menunjukkan identitas 100% dengan Centella asiatica, sementara pegagan merah menunjukkan kemiripan 99,13% dengan Hydrocotile ranunculoides, pegagan embun menunjukkan kemiripan 98,13% dengan Hydroccotyle hyrta, dan kapsul pegagan menunjukkan kemiripan 99,03% dengan Hydrocotyle hyrta. Untuk primer rbcL, pegagan hijau menunjukkan kemiripan 99,81% dengan Hydrocotyle bowlesiodes, dan pegagan merah menunjukkan kemiripan 100% dengan Hydrocotyle vulgaris. Dari penelitian ini dapat disimpulkan bahwa gen matK lebih baik digunakan dalam mengidentifikasi tanaman pegagan karena memiliki kemampuan untuk membedakan tanaman hingga tingkat spesies daripada gen rbcL.
Antimicrobial Activity Test of Ethanol Extract of Forest Betel Leaves (Piper aduncum L.) Diza Sartika; Epi Supri Wardi; Dyah Komala Putri; Miftahur Rahmi
DIVERSITAS HAYATI Vol. 3 No. 2 (2025): DIVERSITAS HAYATI: Journal of Conservation and Biological Resources
Publisher : UIN Sulthan Thaha Saifuddin Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30631/pbpqm618

Abstract

Abstract. Forest betel (Piper aduncum L.) is a herb that is easily found as a wild plant known to contain secondary metabolite compounds, namely flavonoids, tannins, steroids, phenolics and alkaloids. The purpose of this study was to determine the antimicrobial activity of ethanol extract of forest betel leaves (Piper aduncum L.) against Escherichia coli and Staphylococcus aureus bacteria and Candida albicans fungi as well as the minimum inhibitory concentration (MIC) and minimum bacteriside concentration (MBC) of the extract. The method used in the activity test is the Kirby-Bauer method and in the MIC and MBC tests using the optical density (OD) measurement method using a UV / VIS spectrophotometer. The results of testing antimicrobial activity against Escherichia coli bacteria have the largest average inhibition zone at a concentration of 20% (7.86 mm) which is included in the weak category. While in Staphylococcus aureus and Candida albicans, the 80% extract concentration has the largest average inhibition zone, 12.72 mm and 18.54 mm respectively, which is included in the moderate category. Testing the minimum inhibitory concentration (MIC) of the extract against the test microbes showed that in all three test microbes the MIC was obtained at a concentration of 10% extract. testing then continued with the minimum bacteriside concentration (MBC) there were no results because all concentrations on all test microbes showed microbial growth.
Formulation and Antibacterial Activity Test of Hand Soap Gel from Ethanol Extract of Bay Leaf (Syzygium nervosum DC) Noni Rahayu Putri; Epi Supri Wardi; Arnela Sukma Ningsih; Miftahur Rahmi
DIVERSITAS HAYATI Vol. 3 No. 2 (2025): DIVERSITAS HAYATI: Journal of Conservation and Biological Resources
Publisher : UIN Sulthan Thaha Saifuddin Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30631/634vbt26

Abstract

Background: Bay leaf (Syzygium nervosum DC) is one of the plants that has antibacterial activity that can be used as an active substance in hand soap gel preparations of bay leaf ethanol extract. To formulate a hand soap gel preparation from ethanol extract of bay leaf and evaluate its antibacterial activity. This study is experimental in nature. The research includes the preparation of a concentrated extract from bay leaves and its characterization, followed by the formulation of hand soap gel from the ethanol extract of bay leaves in four different concentrations: F1: 7.5%, F2: 10%, and F3: 12.5%. Evaluation of the physical quality of the formulations includes organoleptic properties, homogeneity, pH testing, viscosity testing, and stability testing. The antibacterial activity of the hand soap gel formulation is assessed using the replica method. The physical evaluation results of the hand soap gel meet the SNI standards. The average percentage decrease in the number of colonies was as follows F0 = 31.8%, F1 = 59.4%, F2 = 81.1%, F3 = 83.2% and the comparison preparation (brand B*) was 83.3%. Bay leaf ethanol extract hand soap gel is capable of inhibiting bacterial growth using the replica method.
Co-Authors Achmad Noerkhaerin Putra Agustin, Diana Annisa Annisa Aprila Reza Yuris Arel, Afdhil Aria, Mimi Arnela Sukma Ningsih Aulia Heniken Putri Azyenela, Lola Bastian Nova Dedi Nofiandi Dwi Ditia Zazzora Dyah Komala Putri Etriyel Myh Fadillatul Zikri Fahrul Rozi Fatma Sri Wahyuni Febriani, Icha Fendri, Sandra Tri Juli Hera Purnama Sari Irma Juita, Atika Irwandi irwandi irwandi Irwandi Irwandi Jamsari Jamsari M. Yogi Riyantama Isjoni Miftahur Rahmi Miftahur Rahmi Miftahur Rahmi Mutiara Dita Putri Ningsih, Azma Risilvia Noni Rahayu Putri Nurdianti, Desy Rahmi, Miftahur Ria Afrianti Sari, Atika Aulia Sartika, Diza Satya Wydya Yenny Sentisan Dova Sintia Putri Ayu Sumaryati Syukur Suryani Suryani Tobat, Sanubari Rela Verawati Widyastuti Widyastuti Yolanda Oktaviani Zikri, Fadillatul Zulkarnain Chaidir Zulkarni R, Zulkarni R