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All Journal Jurnal Ilmu Kimia Universitas Brawijaya The Journal of Pure and Applied Chemistry Research JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Medik Veteriner Veterinary Biomedical and Clinical Journal JURNAL KAJIAN VETERINER Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Jurnal Natural
Chanif Mahdi
Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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Journal : The Journal of Pure and Applied Chemistry Research

 1 
Alternative Techniques for Animal Fat Authentication Based on Microscopic Crystal Pattern and Fatty Acid Composition Using Gas Chromatography-Mass Spectrometry (GC-MS) Dewi, Diana Candra; Mahdi, Chanif; Sulistyarti, Hermin; Aulanni`am, Aulanni`am
The Journal of Pure and Applied Chemistry Research Vol. 13 No. 2 (2024): Edition May-August 2024
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2024.013.02.3351

Abstract

Fat authentication is strongly required for the reason of religious food ethics, human health, and preferences. This research reported two simple techniques approach for recognizing fat based on the character of microscopic and physicochemical properties of fat extractedusing different polarity solvents. The formation of microcrystals as well as fatty acid composition were investigated by involving variables of four different types of animal fat and three different solvents. Theisolate fats were directly crystallized under incubation at room temperature and observedusing an optical microscope while fatty acid composition was determined by hydrolyzing and trans-esterifying fat samples before analysis using GC-MS. The microscopic structures of the obtained crystals areneedle-basedwitharadial orientation in spherulitic pattern which characteristic to each type of fat. The lard fat crystal was authentically distinguished from its shape of a partially radial fan, whilst the shape of fat crystals from beef, goat, and lamb are needle-shaped with a full radialorientation. Fatty acids obtained from all types of fats and types of solvents show similar types but different abundances, the main saturated fatty acids are palmitate, stearate myristic and the main unsaturated fatty acids are oleic, palmitoleic, and linoleic. Gas chromatography-mass spectrometry (GC-MS) analysis exhibited that lard composed higher ΣUSFA compared to ΣSFA but other fats (tallow, goat, and lamb) showed converse behavior.
The Influence Water Extract of Black Soybean (Glycine max (L.) Merr.) on Reducing of Blood Glucose Level and The Superoxide Dismutase (SOD) Activity on Diabetes Mellitus Rats Induced With Multiple Low Dose of Streptozotocin (MLD-STZ) Gina, Luh Putu; Mahdi, Chanif; Aulanni’am, Aulanni’am
The Journal of Pure and Applied Chemistry Research Vol. 3 No. 3 (2014)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.775 KB) | DOI: 10.21776/ub.jpacr.%y.03.03.180

Abstract

Black soybean (Glycine max ( L. ) Merr.) is a tropical plant and spread widely in South East Asia including Indonesia. It contains anthocyanins and isoflavones which acts commonly as natural antioxidants. This paper discloses recent investigation on the benefits of its water extract on the oxidative stress in diabetes rat model exposed with multiple low dose of streptozotocin (MLD-STZ). It will also be reported the effect on increasing of the superoxide dismutase (SOD) activity and lowering of blood glucose levels. Groups of male Rattus novergicus strain Winstar was applied as animal model
The Effect of KIO3 and KI Salt Towards Iodium Levels (I2) in Urine, Malondialdehyde (MDA) and The Histology of Thyroid Gland of Goitrogenic Rat Heli, Risman; Mahdi, Chanif; Prasetyawan, Sasangka
The Journal of Pure and Applied Chemistry Research Vol. 3 No. 3 (2014)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (544.921 KB) | DOI: 10.21776/ub.jpacr.%y.03.03.181

Abstract

Goitrogenic substances can inhibit of iodine taking by the thyroid gland. Thus iodine concentration in thyroid gland will be low, and this phenomena is indicated by inflammation in the thyroid gland. Moreover, it can cause releaseing of an excessive amount of free radicals. This radicals, in the body, causes oxidative stress and also increase the levels of malondialdehyde (MDA). This is also as an indicator for lipid peroxidation and the decreasing of urinary iodine excretion levels (EIU). The treatment with KIO3and KI salt was intended to study the level of supplementation of iodine (I2) toward level of MDA in serum and histological description of rat’s thyroid gland. The MDA levels was determined through TBA test (Thio Barbituric Acid), meanwhile the histological pattern of  rat thyroid gland was determined by Hematoxylen-Eosin staining (HE). The results indicated both of KIO3 and KI salt significantly (p<0.01) reduced MDA level in the serum. Treatment with KIO3 salt gave 33.62% while KI salt slightly higher (37,02%). In addition, both of treaments displayed an recovering effect in thyroid gland.
Co-Authors A.A. Ketut Agung Cahyawan W Adrenalin, Sruti Listra Almara, Faniar Tasha Anna Roosdiana Aulanni'am, Aulanni'am Aulanni'am, Aulanni'am Aulanni`am, Aulanni`am Bina, Restu Wika Dewi, Made Artari Diana Candra Dewi Dirgahariyawan, Tri Cahyo Fatra, Ilhami Maulia Fitria Rahmawati Haskito, Ajeng Erika Prihastuti Haskito, Ajeng Erika Prihatuti Hastari, Ayunda Antika Herawati Herawati Hermin Sulistyarti Immauliate, Sinthia Medchera Ira Perdana Wati Janatun Na’imah Julianto, Andri Luh Putu Gina Mardiana, Mardiana Mawarni, Riski Eka Mesla, Wifda Noviatri, Aldila Nugraheni, Putranty Widha Rachmawati Dwi Agustin Rahma, Kartika Rara Anggun Mei Nirbaya Risman Heli Rizki Nur Analita Safitri, Anna Sasangka Prasetyawan Sutrisno Sutisno Vivi Shofia Wardani, Kartika Kusuma Wuragil, Dyah Kinasih