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All Journal Journal of Food and Pharmaceutical Science Jurnal Ilmiah Farmasi Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Dentika Dental Journal Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention The Indonesian Journal of Dental Research Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JFIOnline Media Farmasi Indonesia Jurnal Kefarmasian Indonesia
Sudibyo Martono
Faculty Of Pharmacy, Universitas Gadjah Mada Yogyakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Other

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
Effect of aspirin on pi-class of rat kidney glutathione S-transferase activity Yuniarti, Nunung; Martono, Sudibyo; A.M., Supardjan
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 2, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.919 KB) | DOI: 10.14499/indonesianjpharm0iss0pp87-93

Abstract

The nonsteroids antiinflammatory compounds like curcumin and its analogues, indomethacine, and sulfasalazin have been reported to have an inhibitory effect on GST activity on an in vitro study. The aim of this research is to find out the effect of aspirin on pi-class of rat kidney GST activity in vitro using ethacrynic acid (EA) as a specific substrate for its GST class.Glutathione activity can be measured by conjugating GSH and EA catalyzed with GST. The product can be measured spectrophotometrically to result in a rate (Δ absorption/min). With the same method, aspirin was added as an inhibitor. Decreasing conjugation product indicated that there was an inhibitory activity of aspirin.The aspirin inhibitory activity using EA and CDNB (1-chloro-2,4-dinitrobenzene) as substrates are 9,090% (Extrapolated IC50 6665,03 μM) and 14,087% (Extrapolated IC50 4102,0 μM), respectively. These results have shown that aspirin can not inhibit pi-class of rat kidney cytosolic GST activity using EA and CDNB (1-chloro-2,4-dinitrobenzene) as substrates.Key words: aspirin, GSTP, kidney.
Effect of pentagamavunon-O on rat kidney’s glutathione s-transferase in-vivo with 1-chloro-2, 4-dinitro benzene as a substrate Rohman, Abdul; Martono, Sudibyo; A. Margono, Supardjan
Indonesian Journal of Pharmacy Vol 15 No 1, 2004
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.769 KB) | DOI: 10.14499/indonesianjpharm0iss0pp33-36

Abstract

Glutathione S-transferase (GST) is a detoxifying enzyme which plays an important role in glutathione conjugation reaction with electrophilic xenobiotic on phase two metabolism. In this research, the effect of oral administration of PGV-0 on rat kidney’s GST activity was investigated.To represent GST activity isolated from rat kidney, 1-chloro-2,4-dinitro benzen (CDNB) was used as a substrate on conjugation with glutathione (GSH) spectrophotometrically. The activity of rat kidney’s GSTs from untreated rat was used as control, Based on data, it can be concluded that PGV-0 showed an inhibitory effect on rat kidney’s GSTs. The optimum effect was obtained at 40 mg/kgBW with % inhibition value of 16,16.Key words : Pentagamavunon-0, Glutathione S-transferase, 1-chloro-2,4-dinitro benzene
Analgesic and anti-inflammatory activities of combrang (Nicolaia speciosaHoran) stem the extract Susilowati, Sri Sutji; Martono, Sudibyo; Riyanto, Sugeng; Nugroho, Agung Endro
Indonesian Journal of Pharmacy Vol 22 No 2, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.077 KB) | DOI: 10.14499/indonesianjpharm0iss0pp115-119

Abstract

Long-term  goal  of  this  study  is  the  use  of  combrang  (Nicolaia  speciosa Horan)  as  an  ingredient  of  traditional  medicine  after  being  evaluated  through preclinical  testing  and  clinical  trials  both  extract  and  its  bioactive  compounds. This  study  includes  analgesic-antiinflammatory  activity  test  of  n-hexane, chloroform,  ethyl  acetate  and  methanol  extract  of combrang stem.  Analgesic test against crude extract of n-hexane, chloroform,ethyl acetate and methanol, using acetate writhing method. Antiinflammatory test using inhibition method of paw oedema by carrageenan induced on Wistar rats. Analgesic-antiinflammatory activity  compared  to  Na-diclofenac.  The  result  is  four  extracts  have  analgesic and  antiinflammatory  activity,  ethyl  acetate  extract  has  the  highest  analgesic and antiinflammatory activity.Key words: analgesics, anti-inflammatory, extract of combrang stem
Response Surface Methodology used in the Optimization of RP-HPLC Condition for Quantitative Analysis of Carmine and Rhodamine B Reyna Nuvitasari; Abdul Rohman; Sudibyo Martono
Indonesian Journal of Pharmacy Vol 30 No 4, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm30iss4pp276

Abstract

The objective of this study was to optimize reversed-phase high-performance liquid chromatography (RP-HPLC) using an experimental design approach based on the response surface methodology of Central Composite Design (CCD) for separation and analysis of carmine (CAR) and Rhodamine B (RHO) in lipstick products. Some factors (independent variables) responsible for RP-HPLC separation including pH of buffer phosphate (X1), the acetonitrile ratio (X2), flow rate of mobile phase (X3), and column temperature (X4) were investigated. While, the responses (dependent variables) evaluated were resolution between CAR and RHO (Y1), tailing factor of CAR (Y2), tailing factor of RHO (Y3), retention time of CAR (Y4), retention time of RHO (Y5), peak area of CAR (Y6) and peak area of RHO (Y7). CCD showed that separation of CAR and RHO was influenced by these independent variables (factors). The optimum predicted conditions for the separation of CAR and RHO based on statistical results was pH buffer of 3.4, ACN 55%, the flow rate of 1.1 mL/min and column temperature of 35oC with the desirability of 1. Both CAR and RHO were clearly separated using optimum conditions, as suggested by CCD. The developed techniques were effective for optimizing chromatographic separation, therefore, the time consumption and a large number of running could be hindered.
Co-Authors . Sugiyanto A. Margono, Supardjan Abdul Rohman Abdul Rohman Achmad Fudholi Achmad Fudholi Agung Endro Nugroho Agus Siswanto Agus Siswanto Agustinus Yuswanto, Agustinus Akhmad Kharis Nugroho Anak Agung Istri Sri Wiadnyani Any Guntarti Beti Pudyastuti Endang Lukitaningsih Etty Sulistyowati Harsini Harsini ,, Harsini I Dewa Gde Mayun Permana Iwa Sutardja, Iwa Iwa Sutardjo Iwa Sutardjo, Iwa Marchaban Marchaban, Marchaban Marchaban, . Marchaban, . Melania Perwitasari, Melania Mimiek Murrukmihadi Mochammad Imron Awalludin Nina Salamah Nunung Yuniarti Purwanto Purwanto Reyna Nuvitasari Ririn Sumiyani Riyanto, Sugeng Rohman, Abdul SATRIYAS ILYAS Siti Sunarintyas Siti Zahliyatul Munawiroh Subagus Wahyuono Sudarsono Sudarsono Sugeng Riyanto Supardjan A.M., Supardjan Supardjan Amin M Susilowati, Sri Sutji Wiranti Sri Rahayu Yuny Erwanto