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All Journal Biota TREUBIA Jurnal Layanan Masyarakat (Journal of Public Service) Jurnal Riset Biologi dan Aplikasinya Berkala Penelitian Hayati Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Journal of Bio-molecule Research And Engineering
Affandi, Moch
Department Of Biology, Faculty Of Science And Technology, Airlangga University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Journal : Journal of Bio-molecule Research And Engineering

 1 
Hydrolytic Enzyme Producing Bacteria From The Gut Of Oryctes rhinoceros Larvae Inhabiting Household Waste Composer Ana Mariatul Khiftiyah; Ni'matuzahroh; Mochammad Affandi; Salamun; Silvia Kurnia Sari; Miftahul Jannah; Nastiti Trikurniadewi; Achmad Zainal Abidin; Nurul Wahyuni; Rizki Amaliah Zain; Dela Dwi Alawiyah; Brigita Nur Diyan Agustiana
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.67564

Abstract

relatively shorter adult phase than the larval stage. The larval stage of this organism lives in piles of organic matter, including in piles of household waste. The ability of larvae to utilize organic matter in their environment is inseparable from the role of potential microbes that produce enzymes associated with their gut. This study aims to isolate, screen, and identify the potential bacteria in producing hydrolytic enzymes from gut of O. rhinoceros larvae. The third instar larvae were dissected aseptically to obtain gut bacterial isolates. The isolates were tested for their ability to produce amylase, cellulose, lipase, and protease using agar plate containing specific substrates. There were eight different isolates, and three of them had the ability to produce all four enzymes. The three isolates were identified as Bacillus. These results can be used as a reference for handling household organic waste.
Pycnoscelus surinamensis Endosymbiont Bacteria: Identification and its Amylase Characteristic Ni'matuzahroh; Fatimah; Sholikah; Ayu Tri Wahyuni; Tri Nurhariyati; Mochammad Affandi; Agus Supriyanto; Almando Geraldi; Tini Surtiningsih; Brigita Nur Diyan Agustiana; Dela Dwi Alawiyah; Ana Mariatul Khiftiyah; Silvia Kurnia Sari
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.67571

Abstract

The amylase is an enzyme that widely used in industry. In this research, amylase was isolated from endosymbiont bacteria (EKA 4) of cockroach Pycnoscelus surinamensis from household garbage dump Surabaya. This research aims to identify bacteria EKA 4, produce and characterize amylase from it at various temperature, pH and effect of metal ions. Amylase screening assay was done by imoculating the isolate into medium contain 2% of starch then incubated for 24 hours. Detection of amylase was done qualitatively by measured presence of halozone and quantitatively using DNS method. 16S rRNA analysis found that bacteria EKA 4 has similarity to Bacillus subtilis with 100% of percent identity. Result showed that optimum activity of amylase from B. subtilis were at 40°C, pH 6 and could use ion CaCl2 as a cofactor in a reaction. Thus, utilization of amylase from endosymbiont P. surinamensis could be as an alternative source in big industries.
Co-Authors ACHMAD ZAINAL ABIDIN Agoes Soegianto Agus Supriyanto Alfiah Hayati Almando Geraldi Ana Mariatul Khiftiyah Antien Rekyan Seta Asrifah Ramly , Zakia Asrifah Ramly, Zakia Astra Budi Priatama Aulia Umi Rohmatika Ayu Tri Wahyuni Bambang Irawan Bambang Irawan /E21207042 Binti Mar`atus Solikha Brigita Nur Diyan Agustiana Dela Dwi Alawiyah Eka Kartika Arum Emilia Anjar Prastiwi Fajar Sena Firdausa Fatimah fatimah Fatimah Fitri Citra Arini Hayu Kinanthi Sekar Maharani Iman Dary Supriyadi Putra Indah Fahmiyah Izzatul Millah, Annida Jordan Oktavio Marcelino Lailatul Ayu Fadilah, Risky Leonard Ady Candra Melia E. Agustin MIFTAHUL JANNAH Muhamad Fadhil Mirza Muhamad Hilman Fu'adil Muhamad Maulana Abdizen Nastiti Trikurniadewi Ni'matuzahroh Nimas W. Nastiti Nureka Tiantono NURUL WAHYUNI Puspanjali Prahasto Putri Afin Nurhayati Putut R. Purnama Rini Nur Izza Zuhri, Afifa Rizki Amaliah Zain Rosmanida SALAMUN Salsabila, Syarifah Sholikah Silvia Kurnia Sari Sucipto Hariyanto Sugiarti , Tipuk Tini Surtiningsih Tri Nurhariyati Tri Nurhariyati Trisnadi Widyaleksono Catur Putranto Watamtin Widhiya, Eva