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Ujitoksisitasakut ekstrakbatangpisangambon(Musa paradisiacavar sapientum) terhadap hati mencit (Mus musculus) dengan parameter LD (Acute toxicity test of ambonese banana (Musa paradisiaca var sapientum) stem extract in liver of mice (Mus musculus) with LD 50 parameters 50 Hendrik Setia Budi; Ira Arundina; Retno Indrawati; Leonita Widyana Mahardikasari
Journal of Dentomaxillofacial Science Vol. 13 No. 2 (2014): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v13i2.394

Abstract

The sap of ambonese banana (Musa paradisiaca var sapientum) contain flavonoids, saponins, tannins which have beenwidely used by people in Trunyan Bali as traditional medicine on wound healing, and it has been reported as apotential wound healing after tooth extraction. The aim of this research was to determine the level of safety of usingherbal medicine, ambonese banana stem extract on histopatology liver of mice with LDparameters. This experimentalstudy was performed bythe post test only controlled group design. The sample were 28 mice (Mus musculus) randomlydivided into 4 groups. K group as control group was given aquadest. P1, P2, and P3, as treatment groups were givenambonese banana stem extract with dose 0.42g/20gbw, 2.1g/20gbw, 4.2g/20gbw. The extract was given per-oral withsonde on the first day. On day 3, the mice were terminated, and the livers were microscopically histopathological observed. The observed at 3th50 day, there were no deaths in every groups of mice (K, P1, P2, and P3) on the third day observation. Kruskal Wallis test showed there was not significant difference in histopathological appearance on liverof mice (p=0.771). It wasconcluded that the maximumsafety dose that can still be administered is 0.42g/20gbw followingthe LDparameter. Theacute toxicity test of ambonese banana stem extract did not show necrosis on liver but it showedthe highest simple degeneration than all groups which were given 0.42g/20gbw dose.
againaba Daya Saing Industri Life Sciences di Indonesia Ernawati Pasaribu; Retno Indrawati
Jurnal Aplikasi Statistika & Komputasi Statistik Vol 7 No 2 (2015): Journal of Statistical Aplication and Statistical Computing
Publisher : Pusat Penelitian dan Pengabdian Masyarakat Politeknik Statistika STIS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (814.63 KB) | DOI: 10.34123/jurnalasks.v7i2.22

Abstract

Indonesia is the South East Asia’s largest economy and has a substantial and increasingly inspirational middle class of over 20 million. Indonesia has become an attractive market due to her strongly growing consumer market, especially the middle income segment. The high number of population also indicates the existing potential pool of labour. Life Sciences (LS) industry is widely recognised as the new wave of knowledge-based economy. This study identifies relative position of Indonesia in terms of foreign direct investment (FDI) in LS industry and competitiveness of the LS industry in Indonesia compared with other countries. Based on LS sector, Indonesia has to compete mainly with Portugal, Turkey, Saudi Arabia and Nigeria, while based on LS activities, Argentina and Bulgaria are the main competitors. It also reveals that FDI inflow to LS industry in Indonesia is influenced mainly by inflation and return on investment.
Best Practice in Early Childhood Development Financial Governance: A Case Study in Indonesia Villages Indrawati, Retno; Komara Ragamustari, Safendrri; Ery Wijaya, Muhammad
Jurnal Pendidikan Usia Dini Vol 15 No 2 (2021): Jurnal Pendidikan Usia Dini Volume 15 Number 2 November 2021
Publisher : Program Studi Magister Pendidikan Anak Usia Dini

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.435 KB) | DOI: 10.21009/JPUD.152.07

Abstract

Early childhood development (PAUD) is a fundamental investment that is included in the Sustainable Development Goals (SDGs). Previous research has shown that ECD investment in Indonesia, and its financial governance remains a challenging issue. Through a qualitative approach to a case study method design, this study aims to examine the state of PAUD financial management in Panggungharjo Village, Yogyakarta. This study uses in-depth interviews and desk research as data collection techniques. The instrument was developed from the SABER-ECD World Bank Assessment and ECD Financial Profiling Tools. The results showed that PAUD financial management in established villages had innovative financing, budget management and institutional development resulted in a blueprint for managing PAUD financing in other villages in Indonesia. Important policy objectives in all aspects, including the legal framework, cross-sectoral coordination, program coverage, coverage, equity, data availability, quality standards, and compliance with standards, the vision of financing early childhood development will be more achievable for future research. Keywords: Early Childhood, Financial Governance, Village References: Alatas, H., Brinkman, S., Chang, M. C., Hadiyati, T., Hartono, D., Hasan, A., Hyson, M., Jung, H., Kinnell, A., Pradhan, M., & Roesli, R. (2013). Early childhood education and development services in Indonesia. In Education in Indonesia (pp. 82–108). Institute of Southeast Asian Studies. Ambang.Yudanto. (2019). Analisis Kepemimpinan Transformasional Kepala Desa dalam Pengelolaan Badan Usaha Milik Desa: Studi Kasus Bumdes Panggung Lestari, Bantul [Analysis of Village Head Transformational Leadership in Village Owned Enterprise Management: A Case Study of Bumdes Panggung Lestari, Bantul]. The Journal of Business and Management. Antlöv, H., Wetterberg, A., & Dharmawan, L. (2016). Village Governance, Community Life, and the 2014 Village Law in Indonesia. Bulletin of Indonesian Economic Studies, 52(2), 161–183. https://doi.org/10.1080/00074918.2015.1129047 Aqsati, F. A. (2018). Pengelolaan Alokasi Dana Desa untuk Pengembangan Pembedayaan Masyarakat di Desa Panggungharjo [Management of Village Fund Allocation for Community Empowerment Development in Panggungharjo Village]. Resolusi: Jurnal Sosial Politik. Astuti, S. J. W., & Suaedi, F. (2019). Building Independent Villages through Collaborative Governance by Village-Owned Enterprises (Best Practice from Panggungharjo Village, Central Java, Indonesia). Iapa Proceedings Conference, 265. https://doi.org/10.30589/proceedings.2018.200 Basuki, A. F., Setyowati, K., & Wahyunengseh, R. D. (2019). Accountability Model of Financial Management in the Public Sector: A Study on Panggungharjo Village Budget. Bisnis & Birokrasi Journal. https://doi.org/10.20476/jbb.v26i1.10312 Bauhr, M., & Nasiritousi, N. (2013). Resisting Transparency: Corruption, Legitimacy, and the Quality of Global Environmental Policies. Global Environmental Politics, 13(August), 46–64. https://doi.org/10.1162/GLEP Bloom, N., Van Reenen, J., & Williams, H. (2019). A toolkit of policies to promote innovation. Voprosy Ekonomiki, 2019(10), 5–31. https://doi.org/10.32609/0042-8736-2019-10-5-31 Boggild-Jones, I., Gardiner, S., Gustafsson-Wright, E., Castillo, A. M., Castro Espinosa, B., Sánchez Vázquez, G., Rivera Ruíz, M., Hetzel, O., Lugo, H., Khan, A., Mozambique, F., Duarte, S., Fisker, A., Mozambique, A., Briggs, C., Kasajja, M.-S., Anis, K., Campira, P., Figia, N., … Njoroge, S. (2017). Emily Gustafsson-Wright the Standardized Early Childhood Development Costing Tool (SECT) A Global Good to Increase and Improve Investments in Young Children. Brinkman, S. A., Hasan, A., Jung, H., Kinnell, A., & Pradhan, M. (2017). The impact of expanding access to early childhood education services in rural Indonesia. Journal of Labor Economics, 35(S1), S305–S335. https://doi.org/10.1086/691278 Britto, P. R., Engle, P. L., & Super, C. M. (2013). Handbook of Early Childhood Development Research and Its Impact on Global Policy. In Handbook of Early Childhood Development Research and Its Impact on Global Policy. https://doi.org/10.1093/acprof:oso/9780199922994.001.0001 Cardenas, M., & Cadena, A. M. (2020). How to prioritize Early Childhood? A note on the recent expeerience in Columbia. May 2020. Denboba, A., Hasan, A., & Wodon, Q. (2015). Early Childhood Education and Development in Indonesia. In World Bank Publications. Edi, A. C., & Wardhani, I. S. (2019). Transformational and Transactional Leadership, Understanding How Leadership Cultivates Democratic Citizenship in Panggungharjo, Bantul, Yogyakarta. PCD Journal, 6(2), 239. https://doi.org/10.22146/pcd.35229 Hasan, Amer., Hyson, Marilou., & Chu-Chang, M. (2013). Early childhood education and development in poor villages of Indonesia: Strong foundations, later success. World Bank. Istiqomah, L. (2016). Tiga Pilar Kebijakan Pemerintah dalam Pembinaan PAUD. Golden Age [Three Pillars of Government Policy in Early Childhood Education. Golden Age]: Jurnal Ilmiah Tumbuh Kembang Anak Usia Dini. Jeffrey, D. S., & Guido, S. T. (2014). Financing Sustainable Development: Implementing the SDGs through Effective Investment Strategies and Partnerships. Sustainable Development Solutions Network. Juniar, T. (n.d.). Efektivitas Program Kartu Jakarta Pintar (KIP) Dan Manfaatnya Dalam Meningkatkan Kesejahteraan Sosial Di SDN Bintaro 08 Pagi Jakarta Selatan [The Effectiveness of the Jakarta Smart Card (KIP) Program and Its Benefits in Improving Social Welfare at SDN Bintaro 08 Pagi South Jakarta]. Repository.Uinjkt.Ac.Id. Klees, S. J., Ginsburg, M., Anwar, H., Robbins, M. B., Bloom, H., Busacca, C., Corwith, A., Decoster, B., Fiore, A., Gasior, S., Le, H. M., Primo, L. H., & Reedy, T. D. (2020). The World Bank’s SABER: A Critical Analysis. Comparative Education Review. https://doi.org/10.1086/706757 Kurniawati, S., Suryadarma, D., Bima, L., & Yusrina, A. (2018). Education in Indonesia: A white elephant? Journal of Southeast Asian Economies, 35(2), 185–199. https://doi.org/10.1355/ae35-2e Magnuson, K., & Duncan, G. J. (2016). Can early childhood interventions decrease inequality of economic opportunity? Rsf, 2(2), 123–141. https://doi.org/10.7758/rsf.2016.2.2.05 Mizwar Hasyim, N. (2019). Peningkatan Kemandirian Desa Panggungharjo Melalui Komunikasi Pembangunan [Increasing the Independence of Panggungharjo Village through Development Communication]. Jurnal Pemberdayaan Masyarakat: Media Pemikiran Dan Dakwah Pembangunan, 3(2), 352–376. https://doi.org/10.14421/jpm.2019.032-06 Nakajima, N., Hasan, A., Jung, H., Kinnell, A., Maika, A., & Pradhan, M. (2021). Built to Last: Sustainability of Early Childhood Education Services in Rural Indonesia. Journal of Development Studies, 57(10), 1593–1612. https://doi.org/10.1080/00220388.2021.1873283 National Academies of Sciences Engineering and Medicine. (2018). Transforming the Financing of Early Care and Education. In Transforming the Financing of Early Care and Education. The National Academies Press. https://doi.org/10.17226/24984 Penner, E. K., Rochmes, J., Liu, J., Solanki, S. M., & Loeb, S. (2019). Differing views of equity: How prospective educators perceive their role in closing achievement gaps. Rsf, 5(3), 103–127. https://doi.org/10.7758/RSF.2019.5.3.06 Pratama, R. N., & Pambudi, A. (2017). Kinerja Badan Usaha Milik Desa Panggung Lestari dalam Meningkatkan Pendapatan Asli Desa di Desa Panggungharjon Kecamatan Sewon Kabupaten Bantul [Performance of Panggung Lestari Village-Owned Enterprises in Increasing Village Original Income in Panggungharjon Village, Sewon District, Bantul Regency]. Adinegara. Sayre, R. K., Devercelli, A. E., Neuman, M. J., & Wodon, Q. (2015). Investing in Early Childhood Development: Review of the World Bank’s Recent Experience. In Investing in Early Childhood Development: Review of the World Bank’s Recent Experience. https://doi.org/10.1596/978-1-4648-0403-8 Scheerens, J. (2015). School Effectiveness Research. In International Encyclopedia of the Social & Behavioral Sciences: Second Edition (Second Edition, Vol. 21). Elsevier. https://doi.org/10.1016/B978-0-08-097086-8.92080-4 Suryadarma, D., & Jones, G. W. (2013). Education in Indonesia. Education in Indonesia, 39(3), 1–278. The World Bank. (2013). What Matters Most for Early Childhood Development: A Framework Paper. Systems Approach for Better Education Results (SABER) Working Paper Series, 4–59. Vegas, E., & Santibanez, L. (2009). The Promise of Early Childhood Development in Latin America. In The Promise of Early Childhood Development in Latin America. https://doi.org/10.1596/978-0-8213-7759-8 Zúñiga, N. (2018). U4 Anti-Corruption Helpdesk Does: Does more transparency improve accountability? Transparancy International, 1–13.
ANALYSI S ON SECONDARY I NFECTI ON- TRI GGERI NG MICROORGANISMS IN HIV/AIDS PATIENTS AS A MODEL FOR POLICY CONTROL Rahayu, Retno Pudji; Nasronudin, Nasronudin; Indrawati, Retno; Widiyanti, Prihartini; Lukito, Bimo Dwi; Ferdiansyah, Ferdiansyah; Khairunisa, Siti Qomariyah; Mutamsari, Adiana; Kotaki, Tomohiro
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 4 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.927 KB) | DOI: 10.20473/ijtid.v5i4.2016

Abstract

HIV infection is associated with immune-compromised and rising in opportunistic infection (secondary infection). Therefore, the number of mortality caused by HIV/AIDS is increasing. The use of ARV and development of HIV/AIDS management are expected to suppress the progress of HIV infection into AIDS and, therefore, the mortality can be diminished, while in fact most of the patients eventually suffer from AIDS due to secondary infection that commonly causes death. There should be a management by analysing microorganisms that trigger secondary infection. The method of this study was observational descriptive with cross sectional design. HIV infected blood samples were using ELISA Antibody (IgG and IgM) and Polymerase Chain Reaction (PCR) on laboratory test. The result showed correlation between HIV/AIDS severity and the amount and types of secondary infection. The most common secondary infections were toxoplasm (96.77%), hepatitis C (22.58%), tuberculosis (19.35%), and hepatitis B (3.22%). Other less frequent secondary infections, which were quite difficult to diagnose and not commonly found in Indonesia, were West Nile Virus (25.81%), Japanese Encephalitis Virus (3.22%), and Enterovirus (3.22%). Due to MDGs (Millenium Development Goals) target and the results above, researchers are highly demanded to contribute in decreasing mortality related to AIDS through early detection of secondary infection,including type of infection which have not been commonly found in Indonesia, such as West Nile Virus and Nipah Virus. The discovery of secondary infection in this study was not enough to suppress the occurrence of infection in HIV/AIDS patients. Antimicrobes and good nutrition are required. Moreover, there should be either a primary or secondary prophylaxis to prevent secondary infection that raises the number of mortality and morbidity of HIV/AIDS patients. The result of this study was to meet the target of MDGs by establishing new policies in handling HIV/AIDS infections and have potential as model for policy control in HIV/AIDS.
Rumah Imun dan Ambulan Motor sebagai Upaya Pencegahan Stunting dan Sarana Kegawatdaruratan di Desa Pagung Kabupaten Kediri Sulisetyawati, Theresia Indah Budhy; Roestamadji, Retno Indrawati; Megasari, Ni Luh Ayu; Imannia, Ristya Sylva; Wardhani, Renita Gusti; Maulanasari, Ersa Afifah; Cheardi, Natallensi Deara; Buana, Dwi Candra
Jurnal ABDINUS : Jurnal Pengabdian Nusantara Vol 8 No 2 (2024): Volume 8 Nomor 2 Tahun 2024
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/ja.v8i2.23173

Abstract

This community service aims to improve the skills and knowledge of the surrounding community as well as first aid accommodation to health service facilities in the community of Pagung Village, Semen District, Kediri Regency, East Java. The implementation method of this community service is a field survey to identify stunting problems, making pocket books on emergencies and stunting, making motorbike ambulances (LANTOR) to facilitate access to health services, and counseling related to emergencies, balanced nutrition, and stunting. The results of this community service show that counseling and training related to first aid and emergencies can improve the knowledge and skills of the community in Pagung Village, in addition, the provision of "LANTOR" can facilitate and smooth community mobility to the nearest health service facilities.
Cytotoxicity of Turmeric Extract (Curcuma longa linn.) on BHK21 Fibroblast Cells Azzahra, Nadya Fithrie; Indrawati, Retno; Diyatri, Indeswati
International Journal of Science and Society Vol 6 No 1 (2024): International Journal of Science and Society (IJSOC)
Publisher : GoAcademica Research & Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54783/ijsoc.v6i1.1005

Abstract

Wound healing is the process of recovery from injury to stop bleeding, and fibroblasts play a crucial role in the healing process and contribute to the regeneration of periodontal tissue. Turmeric extract (Curcuma longa linn.) is an alternative treatment that uses natural ingredients with antibacterial, antioxidant, and anti-inflammatory properties. The aim of this study is to demonstrate the toxicity of turmeric extract (Curcuma longa linn.) on BHK21 fibroblast cells. Turmeric extract (Curcuma longa linn.) was administered at concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, and 0.78% to BHK-21 fibroblast cells, and its cytotoxicity was tested using the MTT Assay. The research results show that the percentage viability of fibroblast cells decreases successively with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, and 0.78%, with the highest percentage at a turmeric extract concentration of 0.78% and the lowest at a concentration of 100%. The conclusion of this study is that turmeric extract (Curcuma longa linn.) is not cytotoxic to BHK-21 fibroblast cells at concentrations of 0.78%, 1.56%, and 3.12%.
The Effect of Pluchea indica Less Leaves Extract Againts Biofilm of Enterococcus faecalis and Fusobacterium nucleatum In Vitro Agni Febrina Pargaputri; Elly Munadziroh; Retno Indrawati
Denta Journal Kedokteran Gigi Vol 11 No 1 (2017): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Enterococcus faecalis and Fusobacterium nucleatum are the most common bacteria found in infected root canal teeth and most of them often caused failure in endodontic treatments. These bacteria can form biofilm which makes them more resistant against antibacterial agents. Biofilm formation also causes a decrease in antibiotics and antimicrobials sensitivity. Pluchea indica Less leaves is a species of plants that has several chemical properties. It consists of flavonoids and polyphenols which have benefits to inhibit biofilm formation. Because of its benefits, the extract of Pluchea indica Less leaves can be potentially developed as one of sterilization dressing in root canal teeth. Purpose: The aim of this study was to determine biofilm formation inhibition of Pluchea indica Less leaves extract against Enterococcus faecalis and Fusobacterium nucleatum. Materials and Methods: The dilution method was done first to show the Minimum Inhibitory Concentration (MIC) of the extract. The inhibition biolfilm formation was tested using microtitter plate assay by measuring the bacterial biofilm Optical Density (OD) from ELISA reader’s results and using autoagregation assay to show the inhibition of adherance bacteria. The Pluchea indica Less leaves extract concentration used for inhibition of biofilm formation were 100%, 50%, 25%, 12,5%, and 6,25%. Results: The result of biofilm formation inhibition showed that Pluchea indica Less leaves extract were able to inhibit Enterococcus faecalis and Fusobacterium nucleatum’ biofilm formation with strong moderate effect. The autoagregation assay showed a decrease in autoagregation percentation of Enterococcus faecalis and Fusobacterium nucleatum. Conclusions: Pluchea indica Less leaves extract has effect to inhibit biofilm formation of Enterococcus faecalis and Fusobacterium nucleatum.
Viability and adhesion of osteoblast and fibroblast cells on polymethyl methacrylatehydroxyapatite composite scaffold Wardhani, Karina Kusuma; Roestamadji, Retno Indrawati; Kanawa, Masami; Saskianti, Tania; Basar, Erin Imaniar; Kardikadewi, Varellia Awang Wangi
Indonesian Journal of Dental Medicine Vol. 9 No. 1 (2026): Indonesian Journal of Dental Medicine
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v9i1.2026.30-36

Abstract

Introduction: Polymethyl methacrylate (PMMA) and hydroxyapatite (HA) composites are emerging bone graft candidates, combining mechanical stability with osteoconductivity. PMMA contributes to structural strength and handling properties, while HA enhances bioactivity and bone tissue interaction. Prior to clinical use, biocompatibility toward both hard tissue–related cells (osteoblasts) and soft tissue–related cells (fibroblasts) must be established. Purpose: This study aimed to analyze the cell viability and adhesion of osteoblast (hFOB) and fibroblast (BHK-21) cells on a PMMA-HA composite with a 20:80 ratio. Methods: PMMA-HA composite (20:80) scaffolds were synthesized using the freeze-drying method to obtain a porous structure. Cell viability was evaluated using an indirect contact method with the MTT assay after 24 hours of incubation. Surface topography and cell adhesion morphology were analyzed using Scanning Electron Microscopy (SEM). Results: The MTT assay revealed cell viability percentages of 82.55% for hFOB cells and 88.14% for BHK-21 cells, indicating favorable cellular responses to the PMMA-HA composite. SEM analysis demonstrated that the scaffold possessed a rough, irregular, and porous surface. Cell adhesion analysis showed distinct morphological responses: hFOB cells exhibited a spherical morphology adapting to micropores, while BHK-21 cells displayed extensive spreading with flattened morphology and filopodia extensions. Conclusion: The PMMA-HA composite met biocompatibility standards, showing cell viability above 70% according to ISO 10993-5. The scaffold supported cell adhesion with specific morphological adaptations for both cell lines, indicating that the PMMA-HA composite is non-cytotoxic and a potential candidate for bone graft applications.