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Pengaruh nystatin topikal dan terapi oksigen hiperbarik terhadap jumlah makrofag pada tikus oral candidiasis kondisi imunosupresi Syarifah Fatimatuzzahra; Pargaputri, Agni Febrina; Dwi Andriani; Isidora Karsini
E-Prodenta Journal of Dentistry Vol 7 No 2 (2023)
Publisher : Fakultas Kedokteran Gigi UB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.eprodenta.2023.007.02.7

Latar belakang: Kandidiasis oral merupakan infeksi jamur pada rongga mulut yang sebagian besar disebabkan oleh jamur jenis Candida. Limfosit berperan sebagai pertahanan utama host dalam melawan infeksi jamur pada permukaan mukosa dan epidermis. Penggunaan obat antijamur nystatin efektif untuk pengobatan infeksi jamur, sedangkan terapi oksigen hiperbarik diketahui sebagai salah satu terapi adjuvant dalam mengeliminasi infeksi jamur, sehingga pemberian kombinasi nystatin dan terapi oksigen hiperbarik diharapkan dapat berpengaruh pada sel limfosit dalam melawan infeksi jamur. Tujuan: Membuktikan pengaruh nystatin kombinasi terapi oksigen hiperbarik terhadap jumlah limfosit pada tikus model kandidiasis oral imunosupresi. Metode: Tikus strain Wistar sebanyak 25 ekor dibagi menjadi 5 kelompok: K1 (tikus sehat), K2 (tikus model kandidiasis oral imunosupresi), K3 (tikus model kandidiasis oral imunosupresi diberi nystatin topikal), K4 (tikus model kandidiasis oral imunosupresi diberi terapi oksigen hiperbarik), dan K5 (tikus model kandidiasis oral imunosupresi diberi kombinasi nystatin topikal dan terapi oksigen hiperbarik). Tikus pada K2, K3, K4, dan K5 diimunosupresi dengan deksametason peroral selama 14 hari. Candida albicans dioleskan pada dorsum linguae tikus untuk menginduksi kandidiasis. Nystatin topikal 0,5 cc diberikan pada K3 dan K5, terapi oksigen hiperbarik 2,4 ATA diberikan pada K4 dan K5 selama 5 hari. Jumlah limfosit pada lidah tikus diamati menggunakan mikroskop pembesaran 400x. Hasil: Uji statistik Post-Hoc LSD menunjukkan perbedaan yang bermakna pada kelompok K1 dibandingkan K2, K2 dibandingkan dengan K1, K3, K4, dan K5, sedangkan pada kelompok K3, K4, dan K5 tidak terdapat perbedaan bermakna. Kesimpulan: Kombinasi nystatin dan terapi oksigen hiperbarik berpengaruh pada peningkatan jumlah limfosit model oral kandidiasis imunosupresi.

Correlation between Kupffer Cell Infiltration and Liver Parenchymal Cell Damages in Immunosuppressed Drugs-Induced Rats Pargaputri, Agni Febrina; Wahjuningsih, Endah; Andriani, Dwi
Majalah Kedokteran Bandung Vol 56, No 3 (2024)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v56.3569

The liver is the largest organ in the body, composed of both parenchymal and non-parenchymal cells. Chemical substances and various drugs can induce liver injury and involve Kupffer cells which are non-parenchymal cells that release biologically active substances, promoting pathological processes. This study aimed to evaluate the correlation between the number of Kupffer cells and liver parenchymal cell damages in immunosuppressed, drug-induced rats. The study was conducted from July to December 2019 at the Oral Biology Laboratory of the Faculty of Dentistry and the Biochemical Laboratory of the Faculty of Medicine at Universitas Hang Tuah Surabaya. Twelve healthy male Wistar rats were divided into two groups: Healthy (H) and Immunosuppressed Drug-Induced (ID) groups. Immunosuppression was induced using dexamethasone (0.5 mg/day/rat), administered orally for 14 days, combined with tetracycline (1%/day/rat). Liver samples from all rats were examined for Kupffer cell count and parenchymal cell damages were assessed using a light microscope with 400x magnification. Results revealed a significant difference in the number of Kupffer cells and liver parenchymal cell damages between the H and ID groups (p<0.05). Pearson correlation analysis indicated a significant correlation between Kupffer cell number and parenchymal cell damages (p=0.000). Continuous administration of immunosuppressive drugs may activate Kupffer cells, leading to damage of liver parenchymal cells. In conclusion, the infiltration of Kupffer cells is associated with liver parenchymal cell damages, mediated by various factors in the immunosuppressed drug-induced rat model.

PENGARUH TRAINING OF TRAINER KESEHATAN GIGI DAN MULUT PADA GURU DAN ORANG TUA TERHADAP PERUBAHAN PERILAKU MENGGOSOK GIGI SISWA SISWI SDIT AL-USWAH Pargaputri, Agni Febrina; Andriani, Dwi; Parisihni, Kristanti; Priambodo, Nur Tsurayya; Saptaswari, Dianty; Elidasari, Monika; Fauzia, Bunga; Mulawarmanti, Dian; Sari, Rima Parwati; Kusumawardani, Chaterina Diyah Nanik; Setianingtyas, Dwi; Prananingrum, Widyasri; Nafi'ah; Laihad, Fanny M.; Teguh, Paulus Budi
MAJU : Indonesian Journal of Community Empowerment Vol. 1 No. 6 (2024): MAJU : Indonesian Journal of Community Empowerment, November 2024
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/ss5x9x45

Oral and dental health is an essential aspect of general health that must be maintained from an early age. This study aims to evaluate the impact of the Training of Trainer (ToT) program on dental and oral health for teachers and parents on changing tooth brushing behavior among students at SDIT Al-Uswah Surabaya. The methods used include surveys, training, evaluations, and observations of a tooth-brushing calendar filled out over 21 days. The results showed an increase in knowledge among teachers and parents, as well as positive changes in students' habits of brushing their teeth twice daily. These results highlight the importance of the role of teachers and parents in supporting dental and oral health promotion for children, which is expected to reduce the risk of dental caries in the future.

Effect of hyperbaric oxygen treatment on liver hepatocyte damage in oral candidiasis immunosuppressed rats Pargaputri, Agni Febrina; Andriani, Dwi; Hartono, Mardiyanto Riski; Widowati, Kharinna
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Liver is an organ that performs a role in metabolism and detoxification of chemical substances. The use of immunosuppressive drugs excessively not only become predisposing factor for oral candidiasis, but also can cause damage and affect liverâ€™s function. Hyperbaric oxygen treatment was known to preserve hepatocytes and prevent liver damage. This study was aimed to investigate the effect of hyperbaric oxygen treatment on liver hepatocyte damage in oral candidiasis immunosuppressed rats.Method: We divided fifteen Wistar rats randomly into three groups: G1 (healthy rats), G2 (immunosuppressed rats with oral candidiasis), and G3 (immunosuppressed rats with oral candidiasis given hyperbaric oxygen). The immunosuppressed condition was made by giving dexamethasone and tetracycline orally for 14 days. Induction of Candida albicans 0.1 cc was conducted on the fourth day. Hyperbaric oxygen treatment was given for five days continuously. The histopathological changes in the liver were measured by counting the amount of normal hepatocytes, pycnotic, karyolysis, karyorrhexis, and score of necrosis area using light microscope with 400x magnification.Result: The amount of pycnotic, karyolysis, and karyorrhexis hepatocytes increased in G2 and decreased significantly in G3, likewise the score of necrosis area. The statistical test using oneway Anova and LSD test showed significant differences (p<0,05) in pycnotic, karyolysis, and karyorrhexis hepatocytes between G1, G2, and G3.Conclusion: The hyperbaric oxygen treatment effect on liver hepatocyte damage in oral candidiasis immunosuppressed rats.

PENINGKATAN KEBERSIHAN MULUT MELALUI EDUKASI BERBASIS TOT BAGI GURU DAN ORANG TUA DALAM KEMITRAAN SEKOLAH Sarianoferni, Sarianoferni; Prameswari, Noengki; Hadinata, Yongki; Sumekar, Henu; Paramita, Ayulistya; Lukisari, Cane; Wedarti, Yoifah Rizka; Nafiah, Nafiah; Cevanti, Twi Agnita; Pargaputri, Agni Febrina; Saptaswari, Dianty; Ashrin, Meinar Nur; Hollanda, Ghita Hadi; Aprilia, Aprilia; Setyaningtyas, Dwi
BESIRU : Jurnal Pengabdian Masyarakat Vol. 3 No. 1 (2026): BESIRU : Jurnal Pengabdian Masyarakat, Januari 2026
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/besiru.v3i1.2059

Dental caries in children remains a significant public health problem in Indonesia, particularly among primary school-aged children. Limited oral health literacy and insufficient involvement of teachers and parents in oral hygiene guidance contribute to the high prevalence of dental caries. This study aimed to enhance children’s oral hygiene knowledge and practices through Training of Trainers (ToT)-based education for teachers and parents within a partnership between the Faculty of Dentistry, Hang Tuah University, and El Rahma Islamic Boarding School, Surabaya. A pretest–posttest design was applied, involving fifth-grade students as research subjects. Teachers and parents received ToT-based training delivered by dental professionals and subsequently provided oral health education to students. Knowledge levels were assessed using questionnaires administered before and after the intervention and analyzed using the Wilcoxon Signed-Rank Test. The results demonstrated a significant increase in students’ mean knowledge scores, from 58.14 ± 21.30 at pretest to 77.21 ± 18.30 at posttest (p < 0.05). In addition, partner satisfaction questionnaires indicated a very high level of satisfaction with the program implementation. In conclusion, ToT-based oral health education involving teachers and parents is effective in improving children’s oral hygiene knowledge and has the potential to promote sustainable oral health behavior changes within the school environment.

Effect of hyperbaric oxygen treatment on liver hepatocyte damage in oral candidiasis immunosuppressed rats Pargaputri, Agni Febrina; Andriani, Dwi; Hartono, Mardiyanto Riski; Widowati, Kharinna
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.2.319-326

Background: Liver is an organ that performs a role in metabolism and detoxification of chemical substances. The use of immunosuppressive drugs excessively not only become predisposing factor for oral candidiasis, but also can cause damage and affect liverâ€™s function. Hyperbaric oxygen treatment was known to preserve hepatocytes and prevent liver damage. This study was aimed to investigate the effect of hyperbaric oxygen treatment on liver hepatocyte damage in oral candidiasis immunosuppressed rats.Method: We divided fifteen Wistar rats randomly into three groups: G1 (healthy rats), G2 (immunosuppressed rats with oral candidiasis), and G3 (immunosuppressed rats with oral candidiasis given hyperbaric oxygen). The immunosuppressed condition was made by giving dexamethasone and tetracycline orally for 14 days. Induction of Candida albicans 0.1 cc was conducted on the fourth day. Hyperbaric oxygen treatment was given for five days continuously. The histopathological changes in the liver were measured by counting the amount of normal hepatocytes, pycnotic, karyolysis, karyorrhexis, and score of necrosis area using light microscope with 400x magnification.Result: The amount of pycnotic, karyolysis, and karyorrhexis hepatocytes increased in G2 and decreased significantly in G3, likewise the score of necrosis area. The statistical test using oneway Anova and LSD test showed significant differences (p<0,05) in pycnotic, karyolysis, and karyorrhexis hepatocytes between G1, G2, and G3.Conclusion: The hyperbaric oxygen treatment effect on liver hepatocyte damage in oral candidiasis immunosuppressed rats.

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