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All Journal The Journal of Experimental Life Sciences (JELS) Ulul Albab: Jurnal Studi Islam El-Hayah : Jurnal Biologi El-QUDWAH Prosiding Seminar Biologi Jurnal Biodjati Biosel: Biologi Science and Education Jurnal Biomedika Narra J Berkala Penelitian Hayati Al Karima : Jurnal Studi Ilmu Al Qur'an dan Tafsir Jurnal Kedokteran Hewan Ulil Albab
Bayyinatul Muchtaromah
Department Of Biology, Faculty Of Science And Technology, Universitas Islam Negeri (UIN) Maulana Malik Ibrahim Malang, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Phytochemical Screening and Antibacterial Activity of Acorus calamus L. Extracts Muchtaromah, Bayyinatul; Hayati, Alfiah; Agustina, Erna
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4235

Abstract

  Staphylococcus aureus and Escherichia coli are among the most common species of gram-positive and gram-negative bacteria, which cause vaginitis, in infertile women. The Calamus rhizome (Acorus calamus L.) is an Indonesian plant that has antibacterial properties that can be used to treat vaginitis and increase fertility. The aim of this study was to determine the phytochemical and antibacterial activity of the calamus rhizoma in polar, semi-polar and non-polar solvents in the growth of S. aureus and E. coli. The antibacterial activity test was in the form of inhibitory test using the Kirby-Bauer, Minimum Inhibi-tion Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by microdilution method with multilevel dilution (concentra-tions 50; 25; 12.5; 6.25; 3.13; 1.56; 0.78; and 0.39%). The screening results showed that ethanol and n-hexane extract contained alkaloids and triterpenoids, while chloroform extract was only triterpenoid. Chloroform extract produced the largest inhibition zone diameter of S. aureus and E. coli (7.26 and 3.28 mm), followed by ethanol extract (5.90 and 3.07 mm) and n-hexane extract (5.33 and 2.95 mm). The concentrations of 0.39 and 0.78% were the values of MIC and MBC for all three extracts, indicating that the extract of the calamus rhizome with several solvents in this study had the same antibacterial activity. 
Isolasi dan Karakterisasi Protein 100kDa dari Membran Kepala Spermatozoa Kambing Muchtaromah, Bayyinatul; Sumitro, Sutiman Bambang; Soemarno, Soemarno; Susilawati, Trinil
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1053.644 KB) | DOI: 10.21776/ub.jels.2012.002.01.03

Abstract

Antigen adalah protein yang dapat menginduksi terbentuknya antibodi. Berat molekul protein sebesar 100 kDa dapat bertindak sebagai imunogen yang kuat. Tujuan dari penelitian ini adalah untuk mencari kandidat vaksin imunokontrasepsi dan melakukan isolasi serta karakterisasi protein 100 kDa yang diduga mempunyai peranan penting di dalam interaksi spermatozoa-ovum. Penelitian ini merupakan penelitian eksploratif. Materi Protein 100 kDa diisolasi dari membran spermatozoa kambing menggunakan detergen N-Octyl-glycopiranoside kemudian dirunning menggunakan metode SDS-PAGE dan dikoleksi dengan elektroelusi. Karakterisasi Protein 100 kDa meliputi penentuan berat molekul melalui SDS-PAGE, penentuan titik isoelektrik (pI) dengan IEF (isoelectric focusing) dan kandungan protein dengan metode Biuret. Hasil running isolat protein membran spermatozoa kambing menggunakan metode SDS-PAGE diperoleh pita protein yang terdiri atas 7 pita dengan berat molekul 166,7; 122; 100; 82,8;  60,4; 29,9 dan 14,8 kDa. Isolat Protein 100 kDa diperoleh dengan purifikasi menggunakan metode elektroelusi. Hasil IEF gel elektroforesis Protein 100 kDa dari membran spermatozoa kambing yang diejakulasikan ini mempunyai tiga titik isoelektrik yaitu 6,37; 6,05 dan 5,64. Kandungan protein dalam isolat Protein 100 kDa sebesar 2375 ± 5,65 µg/mL. Hasil ini menunjukkan bahwa isolat protein 100 kDa dapat digunakan sebagai imunogen. Kata Kunci : isolasi, karakterisasi, membrane spermatozoa, protein 100kDa
Co-Authors Ahmad Barizi Alfiah Hayati Ana Mar'a Konita Firdaus Analisa, Izza Analisa, Nur Izzah Ansori, Arif NM. Didik Wahyudi Duhita, Maharani R. Eko Budi Minarno Ely Nuril Fajriyah Erna Agustina Erna Agustina Fajriyah, Ely Nuril Fathorrohman Fathorrohman Fingki Hariyana Firdaus, Ana Mar'a Konita Firdaus, Ana MK. Habibi Habibi Habibi, Soraya Handika Nur Arofik Hasan, M. Nur Jayawarsa, A.A. Ketut LENY RUSVITA UMAMI Lina Ainun Nazilah Maharani Retna Duhita Mei Rhomawati Minarno, Eko B. Mujahidin Ahmad Nayla Alin Naja Nur Izzah Analisa Prilya Dewi Fitriasari Rahmi Annisa Rhomawati, Mei Rizal Maarif Rukmana Romaidi Romaidi Setyowati, Yes Soemarno Soemarno Sofiya Sofiya Soraya Habibi Sutiman B. Sumitro Sutiman B. Sumitro Umaiyatus Syarifah Wira Eka Putra