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All Journal Vegetalika Jurnal Pengendalian Hayati AGRIKA Cropsaver : Journal of Plant Protection MAPETA Agridevina : Berkala Ilmiah Agribisnis Plumula : Berkala Ilmiah Agroteknologi Kultivasi BIOEDUSCIENCE Jurnal Pertanian Agros Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Ilmiah Hijau Cendekia PEDULI: Jurnal Imiah Pengabdian Pada Masyarakat Jurnal Agronomi Tanaman Tropika (JUATIKA) Jurnal Pembelajaran dan Biologi Nukleus Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Agrotropika Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi International Journal on Food, Agriculture and Natural Resources (IJFANRES) Multidiciplinary Output Research for Actual and International Issue (Morfai Journal) Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Tri MUJOKO
Program Studi Agroteknologi, Universitas Pembangunan Nasional ”Veteran” Jawa Timur
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Potential of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. Consortium as an Alternative Soybean Growth Medium Against Ralstonia solanacearum in Vitro Ahmad Davin Najwan Adhyasta; Tri Mujoko; Arika Purnawati
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8263

Abstract

Background: Ralstonia solanacearum is a bacterium highly adaptive to tropical and subtropical environments and can survive for long periods in the soil. This pathogen has a wide host range and is capable of causing severe damage to tomato plants, even leading to total crop failure until 100%. Therefore, appropriate control of these pathogens using biological agents is necessary. Soybean media is considered suitable for propagating Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. due to its adequate nutritional content and economic value. Previous studies have addressed microbes or media separately, such as PDA or NA, whereas this study combines microbial consortia and alternative media in a single integrated test. This study aims to examine the compatibility and antagonistic ability of a consortium of Bacillus sp., P. fluorescens, and Trichoderma sp. cultured on a soybean-based alternative medium against the bacterium Ralstonia solanacearum under in vitro conditions. Methodology: Observations were made on microbial compatibility and inhibition ability against R. solanacearum using thee double layer pepper disk diffusion methode. Findings: The compatibility test results showed that the microbial consortium was compatible and grew optimally on the soybean-based alternative medium. The highest compatibility index (CI) was observed in the Bacillus sp. + P. fluorescens treatment (0.402), while the lowest CI was found in the Trichoderma sp. × Bacillus sp. + P. fluorescens combination (0.873). In the antagonism test, the combination of Trichoderma sp. + Bacillus sp. × P. fluorescens produced an average inhibition zone of 17.2 mm, categorized as strong. Meanwhile, the Bacillus sp. + P. fluorescens treatment had the lowest average inhibition zone of 9.3 mm, categorized as moderate. Contribution: This study indicate that the consortium of Bacillus sp., P. fluorescens, and Trichoderma sp. maintains compatibility and antagonistic activity against R. solanacearum when grown on alternative soybean-based media, which has generally been tested on conventional media. This study recommends the utilization of soybean-based media as an alternative for developing microbial consortia of antagonists in the control of bacterial wilt disease
Effectiveness of Fobio Biopesticide Concentration Against Stem Rot Disease in Vanilla Plants Winadya, Archamiyanis Putri; Wiyatiningsih, Sri; Mujoko, Tri
CROPSAVER Vol 9, No 1 (2026)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v9i1.67869

Abstract

Fusarium oxysporum is a soil-borne pathogen fungus that causes vanilla stem rot, which reduces vanilla productivity. This study aimed to determine the effectiveness of various concentrations of the biopesticide Fobio  in suppressing vanilla stem rot caused by Fusarium oxysporum. This study was conducted in vivo in Tukum Hamlet, Wonosalam District, Jombang Regency, East Java. This study used a Completely Randomized Design (CRD) with four treatments and five replications consisiting of : 5 ml/L (FA), 7.5 ml/L (FB), 10 ml/L (FC), and Control (K). The results showed that 7.5 ml/L was the best concentration, reducing disease intensity to 12.80%, with a biopesticide effectiveness of 51%.
EFFECTIVENESS TEST OF THE APPLICATION OF THE BIOPESTICIDE FOBIO IN CONTROL OF BROWN SPOT DISEASE (CURVULARIA SP.) ON RICE PLANTS (ORYZA SATIVA L.) IN RICE FIELDS IN PUTON VILLAGE, JOMBANG REGENCY, EAST JAVA Ustman Buchori; Sri Wiyatiningsih; Tri Mujoko
Multidiciplinary Output Research For Actual and International Issue (MORFAI) Vol. 6 No. 4 (2026): Multidiciplinary Output Research For Actual and International Issue
Publisher : RADJA PUBLIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.21912308

Abstract

Brown spot (Curvularia sp.) is a plant disease that poses a serious threat to cultivated crops such as rice. This study aims to test the effectiveness of the biopesticide FOBIO in suppressing the intensity of this disease and supporting rice plant growth. The study was conducted in Puton Village, Jombang, using a factorial randomized block design with a combination of FOBIO concentrations (0, 5, and 10 ml/L) and seed soaking times (10, 20, and 30 minutes). The results showed that FOBIO treatment, especially at a concentration of 10 ml/L for 30 minutes, effectively reduced disease intensity and increased plant growth rates. FOBIO has the potential to be an environmentally friendly alternative solution in an integrated pest control system that can be used in the long term.
Biocontrol Potential of Streptomyces sp. from the Avicennia marina Rhizosphere against Sclerotium rolfsii in Bird’s Eye Chili (Capsicum frutescens L.) Yerni Andryani; Arika Purnawati; Tri Mujoko
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20641

Abstract

This study aimed to evaluate the potential of Streptomyces sp. isolated from the rhizosphere of the mangrove Avicennia marina as a biological control agent against Sclerotium rolfsii, the causal agent of basal stem rot in bird’s eye chili (Capsicum frutescens L.). The study was conducted using a non-factorial completely randomized design consisting of a control treatment and root-soaking treatments in a Streptomyces sp. suspension for different durations: 15, 30, and 45 minutes. Observational data were analyzed using ANOVA at the 5% significance level with IBM SPSS Statistics version 27. The results showed that Streptomyces sp. inhibited the growth of S. rolfsii on artificial medium by up to 52.33%, indicating strong antagonistic activity. The in vivo assay further demonstrated that the 45-minute root-soaking treatment (SW3) delayed disease incubation until the final day of observation, prevented the development of disease symptoms throughout the observation period, and enhanced vegetative growth in bird’s eye chili plants. These findings suggest that Streptomyces sp. from the rhizosphere of A. marina has promising potential as a biological control agent for managing basal stem rot caused by S. rolfsii in bird’s eye chili.
Potential of The Biological Agent Bacillus sp. in Inhibiting Moler Disease and its Effects on The Growth and Production of Shallot (Allium ascalonicum L.) F. Tsara Nafisah Yuwono; Yenny Wuryandari; Tri Mujoko
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6428

Abstract

Shallot (Allium ascalonicum L.) is one of the important commodities in Indonesia. The main obstacle that is often faced is moler disease caused by Fusarium sp. Bacillus sp. bacteria have the potential to control Fusarium sp. The purpose of study was to determine the ability of Bacillus sp. in controlling moler disease and its effect on the growth and production of onion plants. The study used a factorial completely randomized design with three replications and nine treatments, so there were 27 experimental units. The observation variables included incubation period, disease intensity, plant height, number of leaves, and tuber wet weight. The research data were analyzed using the ANOVA analysis of variance procedure to determine whether there was an effect of each treatment. If it is known that there is a significantly different effect from the treatment, it will be continued with the DMRT test at the 5 % level. The results showed that the combination of Bacillus sp. isolate bcz 16 and dosage 35 ml/polybag was the longest delaying the incubation period of 15 day and could suppress moler disease by 50 %. The treatment of Bacillus sp. isolate bcz 16 and dosage 35 ml also gave the highest results in plant length, number of leaves, and wet weight of shallot bulbs
Effectiveness of Alternative Media for the Mass Rearing of Trichoderma sp. in Controlling Anthracnose Colletotrichum sp. Disease on Red Chili Plant Maulidya, Aisyah; Purnawati, Arika; Mujoko, Tri
BIOEDUSCIENCE Vol 10 No 2 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/22059

Abstract

Background: Anthracnose caused by Colletotrichum sp. is one of the major diseases limiting red chili production. Trichoderma sp. is a promising biological control agent, but its large-scale application requires suitable propagation media. Methods: This study aimed to evaluate alternative media for the mass propagation of Trichoderma sp. and the suppression of anthracnose through in vitro and in vivo experiments using a Completely Randomized Design (CRD). The in vitro assay involved an antagonism test using five treatments with four replications, measuring the inhibitory effect via the dual culture method. The in vivo assay assessed the incubation period and disease intensity using five treatments with four replications each, yielding 20 experimental units. Each experimental unit consisted of five plant samples, making a total of 100 plants. The collected data were analyzed using ANOVA and the Honestly Significant Difference (HSD) test at a 5% significance level. Results: Alternative media affected the growth and effectiveness of Trichoderma sp. In the in vitro test, inhibition against Colletotrichum sp. ranged from 30.67% to 52.58% at 5 days after inoculation. In the in vivo test, disease intensity at 84 days after planting ranged from 6.25% to 22.50% in treated plants, compared with 28.33% in the control. Corn-rice and rice-bran media showed the most favorable overall performance in supporting propagation and disease suppression. Conclusions: Alternative media can support the propagation of Trichoderma sp. and maintain its antagonistic potential against anthracnose in red chili. Corn-rice and rice-bran media showed the best overall potential among the tested media.
Evaluation of Streptomyces spp. Isolated from the Rhizosphere of Avicennia marina as a Biological Control Agent Against Bacterial Leaf Blight and Their Effects on Rice (Oryza sativa L.) Growth Azzura Talitha Maulidya Irawan; Yenny Wuryandari; Tri Mujoko
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 3 (2026): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i3.22017

Abstract

This study aimed to evaluate the potential of three Streptomyces spp. isolates obtained from the rhizosphere of Avicennia marina at the Mangrove Botanical Garden, Surabaya, for suppressing bacterial leaf blight (BLB) development under in vivo conditions and assessing their effects on plant growth. In a previous study, the three Streptomyces spp. isolates (St8, St9, and St19) demonstrated strong inhibitory activity in in vitro assays. The present study was conducted using a factorial Completely Randomized Design (CRD), with the first factor consisting of Streptomyces spp. isolates (St8, St9, and St19) and the second factor consisting of biological agent suspension doses (5, 10, and 15 ml plant⁻¹). A diseased control treatment was included for comparison. Each treatment was replicated three times, resulting in a total of 30 experimental units. The collected data were analyzed using analysis of variance (ANOVA) with IBM SPSS Statistics 24 software at a 5% significance level. The in vivo efficacy assay showed that the application of Streptomyces spp. isolate St19 at a dose of 5 ml plant⁻¹ only delayed the incubation period of X. oryzae by 1.1 days and was unable to suppress BLB disease severity. The same treatment significantly affected rice tiller number, reaching 13.6 tillers at 56 days after transplanting (DAT), but did not affect plant height throughout the observation period. The results indicate a discrepancy between the in vitro and in vivo assay outcomes. The potential of the tested Streptomyces spp. isolates as biological control agents remains limited; however, they showed a significant effect on rice tiller development.
Co-Authors Ahmad Davin Najwan Adhyasta Ali, Haidar Alvia Cindy Apriliani Arga Dwi Indrawan Ariani Trisna Murti Arika Purnawati Azzura Talitha Maulidya Irawan Bakti Wisnu Widjajani Dewi ANGGRAENI Dita Megasari Eben, Philipus Wahyu Baskoro Edyson Indawan Ekayanti, Savira Aprilia Ersyaf, Ihza Muhammad F. Tsara Nafisah Yuwono Fira Mawardani Firmansyah, Moch Ryan Frimadani, Latifah Harsiyanti, Harsiyanti Haryani, Wiwik Sri Hasni Wardahni Herry Nirwanto Hery Nirwanto, Hery Hidayati Karamina HIDAYATI, KIKI Ika Nur Fitriana Indah Pemuda Jati Laksono, Roy Laras Setyowati Lius, Michelle Lukman Hakim M Rifqi Haikal Makhziah Maulidya, Aisyah Moch Nur Yudha Moch. Arifin Mutiara Alyzza Bilqis Nabila Naylofar Noni Rahmadhini Novita Anggraini Nur Syiami, Aning Nurul Afida Penta Suryaminarsih Penta Suryaminarsih Penta SURYAMINARSIH Penta SURYAMINARSIH Prasetyawati, Endang Triwahyu Pratista, Ariq Dies Purnomo Edi Sasongko RAMADHANI MAHENDRA KUSUMA Ratri, Tesha Lutfika Ratu, Dewanggie Sasmita Risdiyanti, Rateh Lailatul Rulinggar, Nia Salma Alfiani Nadiva Sari, Nadya Naurah Sri Wiyatiningsih Sukartiningrum Sumargono, ' Torino Benarivo Ustman Buchori Vikayanti Vikayanti Widajati, Wiludjeng Wiludjeng Widajati Wiludjeng Widayati Winadya, Archamiyanis Putri Windriyanti, Wiwin Yenny WURYANDARI Yenny Wuryandari Yerni Andryani Zakaria Wildan Fachrezzy