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All Journal Vegetalika Jurnal Pengendalian Hayati AGRIKA Cropsaver : Journal of Plant Protection MAPETA Agridevina : Berkala Ilmiah Agribisnis Plumula : Berkala Ilmiah Agroteknologi Kultivasi BIOEDUSCIENCE Jurnal Pertanian Agros Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Ilmiah Hijau Cendekia PEDULI: Jurnal Imiah Pengabdian Pada Masyarakat Jurnal Agronomi Tanaman Tropika (JUATIKA) Jurnal Pembelajaran dan Biologi Nukleus Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Agrotropika Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi International Journal on Food, Agriculture and Natural Resources (IJFANRES) Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Tri MUJOKO
Program Studi Agroteknologi, Universitas Pembangunan Nasional ”Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Potential of Bacillus spp. Isolates as Biological Control Agents Against Rhizoctonia solani, the Causal Agent of Sheath Rot in Maize (Zea mays L.) Salma Alfiani Nadiva; Yenny Wuryandari; Tri Mujoko
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20689

Abstract

This study aimed to evaluate the effectiveness of laboratory-collected Bacillus spp. isolates against Rhizoctonia solani, the causal agent of maize sheath blight, under both in vitro and in vivo conditions, as available information on their efficacy remains limited. The in vitro assay was conducted using the dual culture method with five Bacillus spp. isolates, namely Bcz-14, Bcz-16, Bcz-20, Bcz-21, and Bcz-30, to assess their inhibitory activity against R. solani. The results showed that all isolates were able to inhibit pathogen growth, although the percentage of inhibition varied among isolates. Isolate Bcz-14 exhibited the highest mean inhibition, while isolates Bcz-16 and Bcz-21 were selected for the in vivo assay based on a combination of mean inhibitory activity and consistency across replications. The in vivo assay was conducted using the two selected isolates with different application times, namely seven days before planting and at planting. The results indicated that treatment B1T1, consisting of Bcz-16 applied seven days before planting, showed a greater tendency to suppress disease development, as indicated by a longer incubation period and the lowest disease intensity of 17.78%. These findings suggest that isolate Bcz-16 has potential for further development as a biological control candidate for maize sheath blight. However, further evaluation under field conditions is required to confirm its effectiveness.
Application of Biopriming Using Secondary Metabolites of Beauveria bassiana in Reducing the Intensity of Fusarium Wilt Disease in Chili Plants Nabila Naylofar; Sri Wiyatiningsih; Tri Mujoko; Vikayanti Vikayanti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.8385

Abstract

Background: Fusarium wilt, caused by Fusarium sp., is one of the main obstacles to chili pepper (Capsicum frutescens L.) production. Disease control using chemical pesticides and fungicides is considered effective in agricultural fields, but excessive use can result in environmental contamination and health risks, including cancer. This makes the application of biological control an effective strategy in overcoming Fusarium wilt disease in chili plants, especially in increasing plant productivity. This study aims to test the effectiveness of biopriming using Beauveria bassiana secondary metabolites in suppressing Fusarium wilt infection in chili seeds in vivo. Methodology: This study used a Completely Randomized Design (CRD). Secondary metabolites were applied to chili seeds at four concentration levels (15%, 25%, 35%, and 45%) as well as positive and negative controls. The treated seeds were planted in a medium inoculated with pathogens and observed for nine weeks. Disease intensity was recorded every seven days and analyzed using ANOVA followed by Tukey's Honestly Significant Difference (HSD) test at the 5% level. Findings:  The results of the variance test 49–63 days after sowing (DAS) showed significant differences between treatments; a further HSD test was conducted at a 5% level. The application of Beauveria bassiana secondary metabolites as a biopriming agent on chili seeds is considered to be ineffective. Contributions: This study provides the first in vivo evidence on the application of Beauveria bassiana secondary metabolites as a seed biopriming agent to suppress Fusarium wilt in chili plants, thereby advancing preventive biocontrol strategies and contributing to the development of environmentally sustainable plant disease management
Co-Authors Ali, Haidar Alvia Cindy Apriliani Arga Dwi Indrawan Ariani Trisna Murti Arika Purnawati Bakti Wisnu Widjajani Dewi ANGGRAENI Dita Megasari Eben, Philipus Wahyu Baskoro Edyson Indawan Ekayanti, Savira Aprilia Endang Triwahyu Prasetyawati Ersyaf, Ihza Muhammad Fira Mawardani Firmansyah, Moch Ryan Frimadani, Latifah Haikal, M Rifqi Harsiyanti, Harsiyanti Haryani, Wiwik Sri Hasni Wardahni Herry Nirwanto Hery Nirwanto, Hery Hidayati Karamina HIDAYATI, KIKI Ika Nur Fitriana Indah Pemuda Indrawan, Arga Dwi Jati Laksono, Roy Laras Setyowati Lius, Michelle Lukman Hakim M Rifqi Haikal Makhziah Moch Nur Yudha Moch. Arifin Mutiara Alyzza Bilqis Nabila Naylofar Noni Rahmadhini Novita Anggraini Nur Syiami, Aning Nurul Afida Penta Suryaminarsih Penta SURYAMINARSIH Penta SURYAMINARSIH Penta Suryaminarsih Pratista, Ariq Dies Purnomo Edi Sasongko Ramadhani Mahendra Kusuma Ratri, Tesha Lutfika Ratu, Dewanggie Sasmita Risdiyanti, Rateh Lailatul Rulinggar, Nia Salma Alfiani Nadiva Sari, Nadya Naurah Sri Wiyatiningsih Sukartiningrum Sumargono, ' Torino Benarivo Vikayanti Vikayanti Widajati, Wiludjeng Wiludjeng Widajati Wiludjeng Widayati Windriyanti, Wiwin Yenny WURYANDARI Yuwono, F. Tsara Nafisah Zakaria Wildan Fachrezzy