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ANALISIS MOLEKULER GEN POTENSIAL PENYANDI SENYAWA FARMASETIKA DARI MIKROALGA ENDOSIMBION PADA JARINGAN ASCIDIAN LISSOCLINUM PATELLA Roring, Verawati Ida Yani
FRONTIERS: JURNAL SAINS DAN TEKNOLOGI Vol 2, No 3 (2019): DESEMBER 2019
Publisher : Universitas Negeri Manado

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Penelitian ini dilakukan bersesuaian dengan program penelitian prioritas Universitas Negeri Manado terutama untuk menjawab berbagai tantangan yang dihadapi bangsa Indonesia dalam mengatasi berbagai problem kesehatan antara lain dalam hal pemenuhan obat-obatan antikanker, telah didisain suatu riset dasar untuk mengeskplorasi potensi molekuler yang terkandung dalam asosiasi mikroalga dan inangnya (Ascidiaceae) dengan pendekatan bioteknologi. Tujuan utamanya mengisolasi dan karakterisasi gen-gen pada mikroalga yang berasosiasi dengan Ascidiciae yang memiliki potensi sebagai sediaan farmasetika, mendapatkan biomassa mikroalga dengan kultivasi, dan mengembangkan metode scalling up perbanyakan mikroalga di luar inang. Tahun pertama penelitian ini isolasi dan kultivasi mikroalga yang berasosiasi dengan Ascidiaceae. Untuk itu telah diadakan pengambilan sampel Ascidian di Pantai Malalayang teluk Manado pada koordinat 1°27?39?LU dan 124°17?31?BT, isolasi mikroalga dan upaya kultivasi di medium Hirata; isolasi DNA mikroalga dan konfirmasi molekuler spesies mikroalga dengan gen penanda filogeni. Walaupun tidak ditargetkan dalam pelaksanaan riset tahun I, namun mendahului mendapatkan gen-gen yang menyandi produk yang memiliki potensi untuk dikembangkan sebagai sediaan farmasi, telah pula dilakukan deteksi metabolomik terhadap sampel mikroalga yang diambil dari alam maupun yang dari hasil kultivasi. Sebagai metabolit dengan berat molekul kecil, komponen metabolomik dapat dimaknai sebagai produk akhir ekspresi gen, sehingga penentuan secara kuantitatif maupun kualitatif dapat menyediakan informasi status biokimia mikroalga karena itu dapat dipakai untuk memantau fungsi gen. Temuan yang diperoleh sampai tahap I berupa hasil kultivasi mikroba simbion, jenis-jenis mebolit dan analisis DNA awal. Luaran penelitian yaitu (1) draft paper untuk publikasi ilmiah di jurnal internasional bereputasi (terindeks Scopus); (2) pemakalah dalam temu ilmiah nasional dan/atau internasional.

PELATIHAN PENGGUNAAN BUAH NAGA SEBAGAI BAHAN PENGAWET ALAMI IKAN CAKALANG PADA SISWA DAN GURU SMP ALEXA LOTTA PINELENG Abigael A M Lahia; Rudi A Repi; Verawati I Y Roring
ABDIMAS: JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 14, No 1 (2021): ABDIMAS: JURNAL PENGABDIAN KEPADA MASYARAKAT
Publisher : LPPM UNIMA

Penelitian ini dilatar belakangi masalah dengan adanya penggunaan zat pewarna sintetis pada ikan cakalang asap , adanya penggunaan bahan pengawet pada ikan cakalang asap dan ikan cakalang fufu merupakan salah satu produk Sulawesi Utara yang banyak dikonsumsi masyarakat. Penelitian ini dilakukan untuk mengetahui pengaruh zat pewarna alami dari kulit buah naga merah terhadap produk ikan cakalang asap dan mengetahui apakah konsumen tertarik dengan produk ikan cakalang asap yang sudah diberikan zat pewarna alami kulit buah naga merah. Metode penelitian ini adalah eksperimen, dengan rancangan percobaan rancangan acak lengkap. Dimana dalam rancangan acak lengkap ini digunakan 5 taraf perlakuan yang diulang sebanyak 3 kali, sehingga total unit perlakuan sebanyak 15 sampel. Konsentrasi uji kontrol, 5%, 10%, 15% dan 20% pemberian zat pewarna alami kulit buah naga merah. uji toksisitas metode BSLT, uji fisik organoleptik metode hedonik. Hasil penelitian menunjukkan uji toksisitas ekstrak kulit buah naga merah menunjukkan bahwa ekstrak kulit buah naga merah tidak toksik dengan nilai LC50 1.1529,042 mg/L, hasil uji organoleptik menunjukkan bahwa pemberian ekstrak kulit buah naga merah pada ikan cakalang asap untuk tingkat kesukaan konsumen pada parameter kenampakan, warna dan rasa yang paling berpengaruh nyata adalah konsentrasi 5% dan 20%.

EFFECT OF FEEDING MARKET VEGETABLE WASTE ON WEIGHT GIAN OF RABBITS (Oryctolagus cuniculus L.) Michael Stiff Kotambunan; Suddin Simanjuntak; Verawati Ida Yani Roring
Indonesian Biodiversity Journal Vol. 3 No. 2 (2022): Agustus 2022
Publisher : Universitas Negeri Manado

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Rabbits are livestock that has the potential to provide meat in a relatively short time, so people's consumption of animal protein is expected to be fulfilled. Market vegetable waste can also be used as feed, especially for rabbits. When processed into rabbit feed, waste is not only a product, but from the feed consumed by rabbits, it can produce meat of good quality. This study aimed to determine whether feeding vegetable waste can increase rabbit body weight and whether combining pellets and vegetable waste can increase rabbit body weight. This study used a quantitative experimental method using an experimental design that was Completely Randomized Design (CRD) with six treatments and four replications. The survey results showed that feeding vegetable waste was effective as feed for rabbits because it could increase the body weight of rabbits (Oryctolagus Cuniculus L.). The more balanced the market vegetable waste feed in the form of cabbage, carrots, pattern (young corn shoots) and pellets were given, the higher the weight gain of rabbits (Oryctolagus Cuniculus L.). Market vegetable waste is not recommended as a good feed. Still, it can be used as a supplement only because research results show that market vegetable waste does not have a significant effect.

ANALISIS KANDUNGAN GIZI DAN UJI BAKTERI COLIFORM PADA TEMPE BIJI BUAH PANGI Pangium edule Reinw Maiko Polandos; Mercy Rampengan; Helen J. Lawalata; Anita C.C. Tengker; Verawati I.Y. Roring
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 8 No. 2 (2023): Bioma : Juli - Desember 2023
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Pangi (Pangiun edule Reinw) merupakan tanaman hutan dan banyak tersebar di wilayah Indonesia. Kelimpahan tanaman pangi belum sejalan dengan pengoptimalam dan pemanfaatannya disebabkan kurangnya informasi akan manfaat cara pengolahan bahan pangan. Penelitian ini dilakukan dengan memanfaatkan biji buah pangi sebagai bahan dasar pembuatan tempe. Tujuan penelitian ini untuk melakukan penelitian mengenai kandungan gizi dan uji bakteri yang terdapat pada tempe biji pangi. Analisis data dilakukan dengan metode deskriptif kuantitatif. Hasil penelitian menunjukkan nilai kadar air 64,96%, kadar abu 0,64%, kadar protein 11,61%, kadar lemak 16,97%, kadar serat 7,20%, kadar karbohidrat 5,82%, dan uji cemaran bakteri menunjukkan nilai 93 APM/g.

ECHINODERM COMMUNITY STRUCTURE IN TAMBA BEACH, NORTH MINAHASA REGENCY, NORTH SULAWESI PROVINCE Jelita Pricilya Tompolumiu; Verawati Ida Yani Roring; Nova Laurin Isye Mourein Ogi; Nonny Manampiring; Helen Joan Lawalata
Indonesian Biodiversity Journal Vol. 4 No. 2 (2023): August 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i2.6391

Tamba Beach has several ecosystems, namely seagrass beds, sandy and rocky. Ecologically, seagrass beds are home to marine life, including Echinodermata. Echinoderms play an important role as an integral part of the food chain, eating organic waste. The purpose of this study was to analyze the community structure of Echinoderms at Station I (Seagrass Zone), Station II (Sandy Zone) and Station III (Coral Zone). Two methods are used in this study. The first is a qualitative description method by directly observing and quantitatively calculating the diversity index, uniformity index, dominance and abundance index formulas. The echinoderms found consisted of 4 classes, namely Holothuidea, Ophiuroidea, Echinoidea and class Asteroidea. The echinoderms found at the study site used a diversity index classified as stable. The dominance index is low because there are no dominant individuals, and the uniformity index is low. From the results obtained from the abundance in Tamba Beach, the highest value was in the Diadema setosum species with 113 individuals. Diadema setosum has the highest quantity at the three existing stations. This species can adapt to environmental changes.

INVENTARISATION OF ORCHID TYPES (Orchidaceae) IN WEST TOMOHON AREA Miracle Abbighael Varencia Welan; Verawati Ida Yani Roring; Helen Joan Lawalata
Indonesian Biodiversity Journal Vol. 4 No. 2 (2023): August 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i2.6585

This study aims to inventory orchid species in the West Tomohon area using the accidental sampling method of exploration in a predetermined place. Orchids are a type of flowering plant that comes from the Orchidaceae family. Each type of orchid has its own characteristics. Tomohon City has abundant natural resources, including ornamental plants such as orchids. Data on orchid diversity in West Tomohon Sub-district has never been reported. Orchid inventory is an activity that records the diversity of orchid species in an area. This research is descriptive-qualitative research. The results of this study have yielded 10 species of orchids belonging to nine genera in one family. The orchid species found in the West Tomohon area consist of Arachnis sp., Arundina graminifolia, Cattleya sp., Dendrobium biggibum, Dendrobium moschatum, Epidendrum secundum, Oncidium flexuosum, Phaius tankervilleae, Phalaenopsis amabilis, and Vanda arcuata. The most common orchid found in this area is the moon orchid, Phalaenopsis amabilis.

EFFECT OF FEEDING MARKET VEGETABLE WASTE ON WEIGHT GIAN OF RABBITS (Oryctolagus cuniculus L.) Michael Stiff Kotambunan; Suddin Simanjuntak; Verawati Ida Yani Roring
Indonesian Biodiversity Journal Vol. 3 No. 2 (2022): Agustus 2022
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v3i2.5737

Rabbits are livestock that has the potential to provide meat in a relatively short time, so people's consumption of animal protein is expected to be fulfilled. Market vegetable waste can also be used as feed, especially for rabbits. When processed into rabbit feed, waste is not only a product, but from the feed consumed by rabbits, it can produce meat of good quality. This study aimed to determine whether feeding vegetable waste can increase rabbit body weight and whether combining pellets and vegetable waste can increase rabbit body weight. This study used a quantitative experimental method using an experimental design that was Completely Randomized Design (CRD) with six treatments and four replications. The survey results showed that feeding vegetable waste was effective as feed for rabbits because it could increase the body weight of rabbits (Oryctolagus Cuniculus L.). The more balanced the market vegetable waste feed in the form of cabbage, carrots, pattern (young corn shoots) and pellets were given, the higher the weight gain of rabbits (Oryctolagus Cuniculus L.). Market vegetable waste is not recommended as a good feed. Still, it can be used as a supplement only because research results show that market vegetable waste does not have a significant effect.

ECHINODERM COMMUNITY STRUCTURE IN TAMBA BEACH, NORTH MINAHASA REGENCY, NORTH SULAWESI PROVINCE Jelita Pricilya Tompolumiu; Verawati Ida Yani Roring; Nova Laurin Isye Mourein Ogi; Nonny Manampiring
Indonesian Biodiversity Journal Vol. 4 No. 2 (2023): August 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i2.6391

Tamba Beach has several ecosystems, namely seagrass beds, sandy and rocky. Ecologically, seagrass beds are home to marine life, including Echinodermata. Echinoderms play an important role as an integral part of the food chain, eating organic waste. The purpose of this study was to analyze the community structure of Echinoderms at Station I (Seagrass Zone), Station II (Sandy Zone) and Station III (Coral Zone). Two methods are used in this study. The first is a qualitative description method by directly observing and quantitatively calculating the diversity index, uniformity index, dominance and abundance index formulas. The echinoderms found consisted of 4 classes, namely Holothuidea, Ophiuroidea, Echinoidea and class Asteroidea. The echinoderms found at the study site used a diversity index classified as stable. The dominance index is low because there are no dominant individuals, and the uniformity index is low. From the results obtained from the abundance in Tamba Beach, the highest value was in the Diadema setosum species with 113 individuals. Diadema setosum has the highest quantity at the three existing stations. This species can adapt to environmental changes.

INVENTARISATION OF ORCHID TYPES (Orchidaceae) IN WEST TOMOHON AREA Miracle Abbighael Varencia Welan; Verawati Ida Yani Roring; Helen Joan Lawalata
Indonesian Biodiversity Journal Vol. 4 No. 2 (2023): August 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i2.6585

This study aims to inventory orchid species in the West Tomohon area using the accidental sampling method of exploration in a predetermined place. Orchids are a type of flowering plant that comes from the Orchidaceae family. Each type of orchid has its own characteristics. Tomohon City has abundant natural resources, including ornamental plants such as orchids. Data on orchid diversity in West Tomohon Sub-district has never been reported. Orchid inventory is an activity that records the diversity of orchid species in an area. This research is descriptive-qualitative research. The results of this study have yielded 10 species of orchids belonging to nine genera in one family. The orchid species found in the West Tomohon area consist of Arachnis sp., Arundina graminifolia, Cattleya sp., Dendrobium biggibum, Dendrobium moschatum, Epidendrum secundum, Oncidium flexuosum, Phaius tankervilleae, Phalaenopsis amabilis, and Vanda arcuata. The most common orchid found in this area is the moon orchid, Phalaenopsis amabilis.

Nutritional Content of Green Algae Caulerpa racemosa and Brown Algae Sargassum polycystum at Ranowangko II Beach, Kombi District, Minahasa Regency Rorong, Joana Hope; Rampengan, Mercy Maggy Franky; Roring, Verawati Ida Yani; Ogi, Nova Laurin Isye Mourein
RUMPHIUS Vol 6 No 1 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i1p041-047

Ranowangko II beach is famous for its beautiful beaches and is a natural habitat for macroalgae, including Caulerpa racemosa and Sargassum polycystum. Despite its great potential and abundance, information about the nutritional content of both types of algae, especially in the Ranowangko II beach area, is still limited. This study aimed to determine the nutritional content (water content, ash content, protein, lipid, crude fiber, carbohydrate) of green algae Caulerpa racemosa and brown algae Sargassum polycystum in Ranowangko II beach. The type of research used is descriptive quantitative to determine the proximate nutrient content of two types of algae tested by proximate analysis in the laboratory. Based on the study results, there were variations in the nutritional content of the two types of algae tested. Green algae Caulerpa racemosa has a moisture content of 71.29%, ash content of 6.90%, protein of 2.78%, fat of 1.34%, crude fiber of 3.53%, and carbohydrates of 17.69%. Brown algae Sargassum polycystum has a water content of 83.23%, ash content of 1.82%, protein of 9.93%, fat of 1.72%, crude fiber of 11.18%, and carbohydrates of 3.30%. The conclusion obtained after conducting this research is that Caulerpa racemosa (green algae) and Sargassum polycystum (brown algae) have different nutritional content.

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