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ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.

SUCCESSION OF LACTIC ACID BACTERIA ON CANDIED PAKOBA FRUIT (Syzygium luzonense) Kembuan, Patricia Pericilia; Mokosuli, Yeremia Samuel; Lawalata, Helen J.; Moko, Emma; Roring, Verawati I. Y
SOSCIED Vol 7 No 2 (2024): SOSCIED - November 2024
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v7i2.878

Pakoba fruit is often made into sweets, salad, pickles because it has a very sour taste and is a habitat for lactic acid bacteria. The purpose of this study was to determine the characteristics of lactic acid bacteria succession during the fermentation process of candied pakoba fruit. This research is an explorative research. The number of microbes was observed starting from day 0, 1, 3 and 7. Based on the results of identification data, 12 bacterial isolates were obtained in pakoba fruit fermentation. The isolates obtained were then macroscopically identified and microscopically identified. 12 isolates were round, 12 isolates had enpitire edges, 12 isolates had raised elevations, 12 isolates had covex colony surfaces, 10 isolates were white and 2 isolates were cream-colored. Of the 12 isolates, it was found that the bacteria that grew in the fermentation of candied pakoba fruit after profile matching was suspected of the genus Lactobacilus sp. and Leucunostoc.

Identification of Escherichia Coli Bacteria in Tofu Soaking Water Sold at Bersehati Market Manado City North Sulawesi Province Salaila, Tania Jennet; Tumbel, Ferny Margo; Lawalata, Helen J.; Tanorl, Meity N.; Roring, Verawati I. Y.
Jurnal Pendidikan Tambusai Vol. 8 No. 2 (2024)
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v8i2.14900

Produk tahu sudah lama menjadi makanan pokok masyarakat Indonesia, terlebih khusus di Kota Manado. Selain harganya murah produk tahu juga sangat banyak dijumpai di tempat-tempat perbelanjaan tradisional maupun swayalan. Tahu memiliki banyak manfaat sebagai sumber protein pengganti dari ikan dan daging. Pada waktu pembuatan tahu biasa kita rendam dengan air terlebih dahulu, karena tahu banyak mengandung protein air rendamannya harus higienis, karena jika tidak higienis akan berpotensi sebagai sarang bakteri pembawa penyakit. Penelitian ini mengenai identifikasi bakteri E.coli pada air rendaman tahu yang diperoleh dari Pasar Bersehati Kota Manado. Air rendaman tahu sudah banyak diteliti, namun belum banyak pengujiannya menggunakan Uji Sitrat, Uji SIM, Uji MR-VP. Penelitian ini bertujuan untuk mengidentifikasi bakteri E.coli di rendaman air tahu. Rancangan penelitian yang digunakan adalah rancangan penelitian deskriptif, mikroskopik, pewarnaan gram, uji biokimia. Hasil penelitian menunjukkan bahwa air yang diambil dari 5 sampel menunjukkan adanya 5 isolat bakteri patogen. Berdasarkan media tumbuh bakteri hanya 3 isolat yang teridentifikasi bakteri E.coli melalui isolasi, pengenceran, pewarnaan gram, dan uji biokimia. Sedangkan 2 sampel tidak teridentifikasi bakteri E.coli. 5 sampel yang telah diteliti ternyata hanya sampel 1,2, dan 4 yang teridentifikasi bakteri E.coli sedangkan sampel 3 dan 5 tidak teridentifikasi bakteri E.coli.

The Influence of the Mind Mapping Model on Students’ Learning Outcomes in Biology Learning at Class XI in Tumou Tou Christian High School, Bitung City Lolaro, Egrina; Roring, Verawati Ida Yani; Nanlohy, Fanny Nella
Jurnal Ilmiah Mandala Education (JIME) Vol 10, No 2 (2024): Jurnal Ilmiah Mandala Educartion (April)
Publisher : Lembaga Penelitian dan Pendidikan Mandala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58258/jime.v10i2.6853

Based on the results of observations and interviews with class This research aims to find out whether the mind mapping model can improve student learning outcomes in plant tissue material in class XI Tumou Tou Christian High School. This research uses a quasi-experimental method (quasi-experiment). The subjects of this research were class XI-A as the experimental class and class XI-C as the control class. Both classes will be given learning outcomes tests in the form of pretest and posttest. Based on the results of data analysis, the results showed that there was an influence of the mind mapping model on student learning outcomes in plant tissue material in class XI Tumou Tou Christian High School. This is based on the results of the t test using IBM SPSS for Windows if the Sig. (2-tailed) < Research Alpha (0.05), namely 0.00 <0.05, then H0 is rejected and H1 is accepted. This shows that there is an influence of applying the mind mapping model on improving student learning outcomes in plant tissue material in class XI Tumou Tou Christian High School.

Identifikasi Teripang Laut (Holothuroidea) dari Perairan Laut Pulau Kabaruan Berdasarkan Identifikasi Morfologi dan DNA Barcoding Rattu, Ferencia Esananda; Mege, Revolson; Manampiring, Nonny; Roring, Verawati Ida Yani; Gedoan, Sukmarayu; Mokosuli, Yermia
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9649

Indonesia is known with its high biodiversity. One of them is sea cucumber. Various species of sea cucumbers are found in Indonesian sea in Talaud Regency at Kabaruan Island. Identification of sea cucumbers to reveal the biodiversity of sea cucumbers in Kabaruan Island, which is almost unknown. This research was to identify six samples of sea cucumber (Holothuroidea) in Kabaruan Island based on morphology and DNA barcoding using the COI gene. The differences between each species can be seen from the morphological characteristics, the length and width of sea cucumbers, papillae, pattern and color. The results showed that there were 4 species from 6 samples, which is Holothuria leucospilota, Holothuria whitmaei, Eleutherozoa sp., and Holothuria atra. That six samples with the code A1, A2, B1, C1, and C2 have a homology level of 99%-100% with the target nucleotide sequence from GenBank. Meanwhile, the sampleâ€™s code A3 has a homology level of 90% with the Eleutheroza sp. so there is a possibility that sample A3 is a new taxa that has the highest similarity to the Eleutheroza sp. From the six samples, it was found that samples A2 and B1 had the highest level of similarity which is 100%. Meanwhile, the samples with the lowest percentage values are samples A3 and B1 and samples A2 and A3, with a percentage value of 82.75%. There were 4 species from six samples found in Kabaruan Island. There is a possibility that Sample A3 is a new species that is similar to Eleutheroza sp.

APPLICATION OF Eco-Enzyme CONVERSION RESULTS INTO DISHWASHER SOAP Tedang, Bybyani; Roring, Verawati Ida Yani; Moko, Emma Mauren; Posumah, Danny Christian; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10752

The application of the conversion results from Eco-Enzyme into dishwashing soap, the fermentation process lasts for one months, where the resulting liquid is dark brown in color and has a very strong sweet and sour fermented aroma. There is a lack of public knowledge about the very profitable benefits of Eco-Enzyme, such as as a multi-purpose cleaner as a multi-functional liquid which can be converted, one of the ways, into dishwashing soap. This research aims to determine the application of the results of converting Eco-Enzyme into dishwashing soap. This research used experimental methods and a research period of 3 months. This Eco-Enzyme is made with a ratio of organic ingredients: sugar: water 3:1:10. The Eco-Enzyme harvest is converted into dishwashing soap by adding lerak and Methyl Ester Sulfonate (MES). Eco-Enzyme conversion soap was then applied to several treatment groups: plastic plates, cans and glass. The results of the application show that the dishes washed with Eco-Enzyme using lerak have not completely removed dirt, oil and odors in each treatment, whereas Eco-Enzyme using Methyl Ester Sulfonate (MES) when compared with Sunlight brand liquid soap sold on the market shows that there are similarities both in terms of cleanliness, tapestry and smell. The research conclusion is that the application of the Eco-Enzyme conversion results has significant potential to be developed into an active ingredient for environmentally friendly dishwashing soap.

ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.

APPLICATION OF Eco-Enzyme CONVERSION RESULTS INTO DISHWASHER SOAP Tedang, Bybyani; Roring, Verawati Ida Yani; Moko, Emma Mauren; Posumah, Danny Christian; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10752

The application of the conversion results from Eco-Enzyme into dishwashing soap, the fermentation process lasts for one months, where the resulting liquid is dark brown in color and has a very strong sweet and sour fermented aroma. There is a lack of public knowledge about the very profitable benefits of Eco-Enzyme, such as as a multi-purpose cleaner as a multi-functional liquid which can be converted, one of the ways, into dishwashing soap. This research aims to determine the application of the results of converting Eco-Enzyme into dishwashing soap. This research used experimental methods and a research period of 3 months. This Eco-Enzyme is made with a ratio of organic ingredients: sugar: water 3:1:10. The Eco-Enzyme harvest is converted into dishwashing soap by adding lerak and Methyl Ester Sulfonate (MES). Eco-Enzyme conversion soap was then applied to several treatment groups: plastic plates, cans and glass. The results of the application show that the dishes washed with Eco-Enzyme using lerak have not completely removed dirt, oil and odors in each treatment, whereas Eco-Enzyme using Methyl Ester Sulfonate (MES) when compared with Sunlight brand liquid soap sold on the market shows that there are similarities both in terms of cleanliness, tapestry and smell. The research conclusion is that the application of the Eco-Enzyme conversion results has significant potential to be developed into an active ingredient for environmentally friendly dishwashing soap.

Application of the POE Learning Model to Improve Biology Learning Outcomes in Grade VII Students Ferny M Tumbel; Verawati I. Y. Roring; Yustita Slamat
Edu Cendikia: Jurnal Ilmiah Kependidikan Vol. 5 No. 01 (2025): Research Articles, April 2025
Publisher : ITScience (Information Technology and Science)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/educendikia.v5i01.5698

This research explores using the Predict, Observe, and Explain (POE) model to enhance the learning process in elementary schools, particularly in biology lessons. The study conducted at SMP Berea Tondano with class VII focuses on how the POE model affects student understanding and engagement, specifically in classifying living organisms. A classroom action research (CAR) approach was employed in two cycles, each involving planning, action, observation, and reflection. Nine students from class VII participated in the study. Data were collected through observations, student activity sheets, and tests at the end of each cycle to evaluate the impact of the POE model on learning outcomes and student engagement. The research findings revealed significant improvements in student engagement and learning outcomes across the two cycles. In the first cycle, 55.56% of students achieved the learning goals, which increased to 88.89% in the second cycle. Similarly, student activity levels rose from 35.71% in the first cycle to 78.57% in the second cycle, reflecting greater participation and motivation. The study concludes that the POE model effectively enhances student learning outcomes and promotes active engagement. By involving students in prediction, observation, and explanation, the POE model aids in better understanding and retention of biological concepts. Further research is suggested to explore its application in other subjects and educational contexts.

PEMANFAATAN LIMBAH ORGANIK RUMAH TANGGA MENJADI CAIRAN MULTIGUNA ECO ENZYME: Utilization of Household Organic Waste Becomes Cairan Multiguna Eco Enzyme Wantania, Johanna Zusye; Roring, Verawati Ida Yani; Rahardiyan, Dino; Moko, Emma Mauren; Rawung, Livana Dethris
Vivabio: Jurnal Pengabdian Multidisiplin Vol. 7 No. 2 (2025): VIVABIO: Jurnal Pengabdian Multidisiplin
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/vivabio.v7i2.64366

Household waste contributes the largest share of daily waste production, necessitating waste management and the participation of women, as those responsible for managing household waste. Eco Enzyme is a product processed from organic waste to produce a multi-purpose liquid that is highly beneficial for women as housewives. The purpose of this activity is to manage waste through the production of eco-enzyme for women in the Sion Ranomut Congregation. This activity increases the knowledge and skills of women in waste management. Furthermore, this activity serves as a means of dissemination and training for women, fostering their enthusiasm and love for the environment at home and beyond. It also serves as a platform for lecturers to implement knowledge and conduct off-campus activities to achieve the Key Performance Indicators for Universities and Campuses with Impact. ABSTRAK Sampah rumah tangga menyumbang jumlah terbesar terhadap produksi sampah sehari-hari sehingga perlu tindakan pengolahan sampah dan partisipasi dari Wanita Kaum Ibu yang merupakan salah satu yang bertanggung jawab untuk mengelola sampah hasil kegiatan rumah tangga. Eco Enzyme merupakan salah satu produk olahan dari limbah organik untuk menghasilkan cairan serbaguna yang sangat bermanfaat untuk kebutuhan wanita kaum ibu sebagai ibu rumah tangga. Tujuan kegiatan ini adalah pengelolaan sampah dengan pembuatan melalui pembuatan Eco Enzyme bagi wanita kaum ibu di Jemaat Sion Ranomut. Melalui kegiatan ini terjadi peningkatan pengetahuan dan ketrampilan wanita kaum ibu tentang pengelolaan sampah, selain itu kegiatan ini menjadi media diseminasi dan pelatihan bagi wanita kaum ibu dalam menumbuhkan semangat dan kecintaan wanita kaum ibu terhadap lingkungan di rumah dan sekitarnya serta bagi dosen dalam implementasi ilmu pengetahuan serta melakukan kegiatan luar kampus dalam upaya pencapaian Indikator Kinerja Utama Perguruan Tinggi dan Kampus berdampak.

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