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All Journal Microbiology Indonesia Jurnal Labora Medika Jurnal Biomedika Conference on Innovation in Health, Accounting, and Management Sciences Proceeding Jurnal Masyarakat Madani Indonesia

Nony Puspawati

Program Studi D4 Analis Kesehatan Fakultas Ilmu Kesehatan, Universitas Setia Budi Surakarta

Author-ID : 331448

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences

Published : 18 Documents Claim Missing Document

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The Sensitivity Test of Mycobacterium tuberculosis to Snail Seromucoid and Chitosan in vitro Yusup Subagio Sutanto; Magdalena Sutanto; Agnes Sri Harti; Nony Puspawati
Biomedika Vol 14 No 1 (2021): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v14i1.1128

Tuberculosis (TB) is an infection caused by M. tuberculosis (MTb) and is transmitted through droplets of phlegm in the air from patients or those suspected of having TB. In general, treatment for TB is done with anti-tuberculosis drugs (ATDs), specifically streptomycin, isoniazid, rifampicin, and ethambutol (SIRE) that takes a long time due to the level of resistance of MTb bacteria. The resistance of MTb triggers ATDs based on natural bioactive compounds. Chitosan as a result of chitin deacetylation can function as an antimicrobial agent because it is polycationic, which is biodegradable, biocompatible, and non-toxic. Snail (Achatina fulica) seromucoid contains antibacterial bioactive compounds, namely glycans, peptides, glycopeptides, achasin protein, and chondroitin sulfate. This study aims at testing the sensitivity of MTb isolates against snail seromucoid and chitosan in vitro. This research applied the experimental research method. MTb isolates were obtained from sputum samples of patients suspected of TB at the Surakarta Regional Public Hospital (RSUD Surakarta). The results of screening for MTb were positive, based on the microscopic examination of MTb using the Ziehl Nelson (ZN) method, the MPT 64 rapid test, and the quick molecular test using the Genexpert method. The research was completed through several stages, including the preparation of a suspension of germs with a concentration of 1 mg/ml or Mc. Farland 0.5-1.0; preparation of the stock solution and working solution (WS); drug sensitivity test (DST) against snail seromucoid; chitosan and ATDs (SIRE) on Lowenstein Jensen (LJ) media; and incubation at 37°C for 3-4 weeks. The results were interpreted on day 28 or day 42. The results have revealed that MTb isolates are 100% resistant to snail seromucoid and 2% chitosan. This study concludes that MTb isolates from suspected TB are resilient to 100% snail seromucoid and 2% chitosan.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOLIK DAUN KELOR (Moringa oleifera Lamk.), DAUN TEH HIJAU (Camellia sinensis L.), DAUN BINAHONG (Anredera cordifolia (Tenore) Steen.), DAN MENIRAN HIJAU (Phyllanthus niruri L.) TERHADAP Pseudomonas aeruginosa ATCC 27853 Iloh Antarini; Nony Puspawati; Rahmat Budi Nugroho
Jurnal Labora Medika Vol 5, No 2 (2021): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Some plants such as Moringa, green tea, binahong, and green meniran can be used as alternative antimicrobials because they are proven to contain antibacterial compounds. This study aimed to determine the antibacterial activity of the ethanolic extracts of Moringa leaves, green tea leaves, binahong leaves, and green meniran against Pseudomonas aeruginosa ATCC 27853. The extracts were obtained by maceration method with 70% ethanol solvent and tested for their antibacterial activity using the well diffusion method with a concentration of 60%. The results showed that the ethanolic extracts of Moringa leaves, green tea leaves, binahong leaves, and green meniran had antibacterial activity against Pseudomonas aeruginosa ATCC 27853 with inhibitory powers of 20.6 mm, 17.3 mm, 15.6 mm, and 22.6 mm. Meniran green ethanolic extract has the most active antibacterial activity.

Identifikasi Salmonella Sp dan Gambaran Histopatologi Hati Ayam yang Terinfeksi Salmonella di Pasar Tradisional Kota Boyolali Lila Fadilla; Nony Puspawati; Rahmat Budi Nugroho
Conference on Innovation in Health, Accounting and Management Sciences (CIHAMS) Vol. 1 (2020): Proceeding 1st Setia Budi Conference on Innovation in Health, Accounting, and Managem
Publisher : Conference on Innovation in Health, Accounting and Management Sciences (CIHAMS)

Salmonella sp merupakan bakteri yang daapt menyebabkan foodborne disease di berbagai negara terutama di negara berkembang. Salmonella sp dapat dijumpai pada saluran percernaan manusia maupun hewan. Penularannya dapat melalui makanan yang tercemar, daging, telur dan susu. Histopatologi merupakan ilmu yang mempelajari tentang kondisi dan fungsi jaringan yang dihubungkan dengan penyakit. Tujuan dari penelitian ini untuk mengetahui ada atau tidaknya bakteri Salmonella sp pada hati ayam dan gambaran struktur jaringan hati ayam yang terinfeksi dan tidak terinfeksi. Sampel diambil secara acak dipasar tradisional di kota Boyolali kemudian dilakukan dilakukan pemeriksaan mikrobiologi dan pemeriksaan histopatologi dengan pengecatan Hematoksilin Eosin. Hasil penelitian ini menunjukan bahwa dari 5 sampel yang diteliti, didapatkan hasil 3 positif Salmonella sp meskipun secara kasat mata hati ayam tidak memiliki perubahan fisik yang menentukan adanya bakteri Salmonella sp. Sedangkan pada hasil pemeriksan histopatologi tidak ada beda antara hasil gambaran struktur jaringan hati ayam yang terinfeksi dan tidak terinfeksi Salmonella sp, hasil ini menunjukan bahwa kerusakan pada sel jaringan pada sampel bukan diakibatkan oleh infeksi bakteri Salmonella sp.

Deteksi Escherichia coli dengan Metode Polimerase Chain Reaction (PCR) Yolanda Kharisma Setia; Nony Puspawati; Rizal Maarif Rukmana
Conference on Innovation in Health, Accounting and Management Sciences (CIHAMS) Vol. 1 (2020): Proceeding 1st Setia Budi Conference on Innovation in Health, Accounting, and Managem
Publisher : Conference on Innovation in Health, Accounting and Management Sciences (CIHAMS)

Escherichia coli adalah Bakteri patogen yang dapat menyebabkan diare dan termasuk dalam kelompok enterohemoragic yang dapat menimbulkan penyakit hemorrhagic colitis ditandai dengan diare berdarah. Bakteri Escherichia coli memiliki beberapa subtipe penyebab diare diantaranya Escherichia coli enteropatogenik (EPEC), Escherichia coli enterohemorrhagic (EHEC), Escherichia coli enterotoksigenik (ETEC), Escherichia coli enteroaggregatif (EAEC), Escherichia coli enteroinvasive (EIEC) dan Diffusely adherent Escherichia coli (DAEC). Polimerase Chain Reaction (PCR) adalah metode amplifikasi DNA secara in vitro. Tujuan penelitian ini adalah untuk mengetahui Escherichia coli dapat dideteksi menggunakan Polimerase Chain Reaction (PCR) dan unruk mengetahui jenis Escherichia coli yang dapat terdeteksi menggunakan Polimerase Chain Reaction (PCR). Penelitian ini menggunakan pendekatan literatur review yang berfokus pada evaluasi beberapa hasil penelitian sebelumnya yang berkaitan dengan sumber literatur yang digunakan. Proses pencarian literatur dengan menyebutkan kata kunci “Deteksi Escherichia coli dengan metode Polimerase Chain Reaction (PCR). Hasil dari literatur review menunjukan bahwa bakteri Escherichia coli dan bakteri Escherichia coli O157:H7 Escherichia coli enteropatogenik (EPEC), Escherichia coli enterohemorrhagic (EHEC), Escherichia coli enterotoksigenik (ETEC), Escherichia coli enteroaggregatif (EAEC), Escherichia coli enteroinvasive (EIEC) dan Diffusely adherent Escherichia coli (DAEC) dapat dideteksi menggunakan Polimerase Chain Reaction (PCR).

Antimicrobial Bioactive Compounds of Snail Seromucoid as Biological Response Modifier Immunostimulator AGNES SRI HARTI; NONY PUSPAWATI; RAHAJENG PUTRININGRUM
Microbiology Indonesia Vol. 13 No. 2 (2019): June 2019
Publisher : Indonesian Society for microbiology

Anti-microbial bioactive compounds from snail (Achatina fullica Ferussac) contained in snail seromucoid. It contains bioactive compounds such as glycans, peptides, glycopeptides, and chondroitin sulfate which can function as biological response modifiers (BRM) immunostimulators. Immunostimulators are compounds that can increase cellular immune responses in various ways, namely increasing the number and activity of T cells, NK cells, and macrophages and releasing interferons and interleukin. Immunostimulators are compounds that can increase cellular immune responses in various ways, namely increasing the number and activity of T cells, NK cells, macrophages and releasing interferons and interleukins.The purpose of this study was to analyze antimicrobial bioactive seromucoid compound of snail (Achatina fullica Ferrusac) as biological response modifiers (BRM) immunostimulators. The research methods based on experimental laboratory results with research stages including snail seromucoid isolation; antimicrobial activity; characterization physicochemical and profile of snail seromucoid proteins. The results of antimicrobial activity showed that 100% seromucoid concentrations had MIC (Minimal Inhibition Concentration) in Staphylococcus aureus, Candida albicans, and Pseudomonas aeruginosa. The physicochemical examination results showed specific gravity of 1.010; pH 8, -1 -1 -1g lucose 16 mg dL ; 9 mg dL cholesterol; protein 2.8 mg dL and negative heavy metals (Pb, Cu, Hg, Al). The results of the analysis of protein profiles showed that there were 3 subunits of proteins, range from 55 to 72 kDa and 1 specific protein sub unit of 43 kDa which was thought to be antimicrobial and biological response modifiers (BRM) immunostimulators.

IDENTIFIKASI DAN UJI SENSITIVITAS Staphylococcus aureus TERHADAP ANTIBIOTIK DARI SAMPEL ULKUS PASIEN DIABETES MELITUS DI RSUD Dr. MOEWARDI Masita, Tutus Elsa; Puspawati, Nony; Rahmat Budi Nugroho
Conference on Innovation in Health, Accounting and Management Sciences (CIHAMS) Vol. 3 (2023): Proceeding 3rd Setia Budi Conference on Innovation in Health, Accounting, and Managem
Publisher : Conference on Innovation in Health, Accounting and Management Sciences (CIHAMS)

Show Abstract | Download Original | Original Source | Check in Google Scholar

The prevalence of Diabetes Mellitus in Indonesia is increasing, Uncontrolled blood glucose levels may lead to diabetic ulcers, which in turn can lead to peripheral neuropathy. The bacterium Staphylococcus aureus is a possible source of this illness. Staphylococcus aureus has become a major health risk due to the rise in bacterial resistance to many classes of antibiotics, making sensitivity testing imperative. The purpose of this investigation is to identify Staphylococcus aureus in ulcer samples from RSUD Dr. Moewardi's diabetic patients and to investigate their susceptibility to various antibiotics. This type of research is an analytic observational approach cross sectional namely research to determine the sensitivity of bacteria to antibiotics by the diffusion method with Kirby-Bauer discs. This research was conducted by isolating bacteria Staphylococcus aureus in ulcer samples of patients with diabetes mellitus and then tested for sensitivity to antibiotics. The results of the ulcer sample isolation showed that out of 10 samples there were 4 positive ulcer samples Staphylococcus aureus. Sensitivity test results show that Staphylococcus aureus 100% sensitive to the antibiotics Ciprofloxacin, Imipenem, Gentamicin, and Vancomycin, 90% sensitive to the antibiotic Erythromycin, while 10% is intermediate to the antibiotic Erythromycin

The Potential and Effectiveness of Snail Seromucoid and Chitosan as Bioimmunostimulators Harti, Agnes Sri; Sutanto, Yusup Subagio; Puspawati, Nony
Biomedika Vol 15 No 2 (2022): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v15i2.1448

The host's cellular immune response plays an important role in the process of eliminating microorganisms that cause infection. Substances that can stimulate an increase in the immune response are called immunostimulators. Snail seromucoid contains bioactive compounds such as glycans, peptides, glycopeptides and chondroitin sulfate. Chitosan as an antimicrobial agent can be used in the biomedical field because chitosan has a number of hydroxyl groups (OH) and amine groups (NH2). The research objective was to determine the potency and effectiveness of snail seromucoid and chitosan as bioimmunostimulators. The research method is based on laboratory experimental results with the research stages, namely the analysis of the effectiveness of seromucoid and chitosan on lymphocyte proliferation. The results of the one way ANOVA analysis showed a p value of 0.000 so that there was a significant effect between the treatment groups, namely the effect of giving chitosan 65 ug/ml; snail mucus 65 ug/mL and a combination of chitosan (65 ug/mL) and snail mucus (65 ug/mL) ratio of 1:1, can increase lymphocyte proliferation optimally. This shows that snail mucus, chitosan and their combination in a 1:1 ratio are effective as BRM (Biological Response Modifier). It is hoped that the contribution of the results of this study can be further developed in the bioformulation of snail seromucoid preparations and chitosan as therapeutic agents for infectious diseases, including Acute Respiratory Distress Syndrome (ARDS), Tuberculosis and other diseases.

Edukasi Kesehatan dan Implementasi Produk Krenova Krim Galenik Untuk Perawatan Luka Melalui Optimalisasi Pemberdayaan Kemitraan Masyarakat Harti, Agnes Sri; Syukur, Bambang Abdul; Sutanto, Yusup Subagio; Puspawati, Nony
Jurnal Masyarakat Madani Indonesia Vol. 4 No. 3 (2025): Agustus
Publisher : Alesha Media Digital

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59025/5m5ga075

Penerapan hasil riset civitas akademika Universitas Kusuma Husada Surakarta terkait eksplorasi potensi bahan alam dan pengembangn teknologi formulasi sediaan galenik berbasis bahan alam perlu dioptimalkan untuk menunjang efektivitas terapi perawatan luka. Produk krenova krim galenik untuk perawatan luka berbasi seromukoid bekicot 1%, ekstrak kulit durian 1% dan kitosan 1%, telah terdaftar Paten Sederhana No. S00202214385 dan No. S00202010479. Tingginya kasus luka kronis disebabkan paradigma yang masih ada di masyarakat khususnya di Posyandu RW 16 Ceplukan, Desa Wonorejo, memberikan gambaran perlunya dilakukan edukasi kesehatan bagi masyarakat terkait permasalahan perawatan luka. Tujuan kegiatan melakukan edukasi kesehatan dan implementasi produk krenova untuk perawatan luka berbasis knowledge based economy, healthy and safety product melalui optimalisasi pemberdayaan kemitraan masyarakat. Metode pelaksanaan berupa edukasi kesehatan menggunakan leaflet, diskusi dan tanya jawab serta evaluasi kegiatan melalui pre dan post kuisioner tentang perawatan luka terhadap 30 peserta. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan terhadap materi manajemen perawatan luka yaitu peserta memiliki pengetahuan baik pre test 5 peserta (16,7%) dan post test 21 peserta (70). Implementasi esensi dan kebermanfaatan hasil riset yaitu terjalinnya kolaborasi kemitraan link and match dengan stake holder, DUDI dan masyarakat guna mendukung suksesnya program Pemerintah untuk menunjang efektivitas terapi perawatan luka kronis.

Co-Authors Agnes Sri Harti Agnes Sri Harti Agnes Sri Harti Argoto Mahayana Argoto Mahayana Aulia Wahyu Sulviana Bambang Abdul Syukur Dewi Astuti Herawati Dewi Sulistyawati Diah Mukti Cahyaningtyas Dionysius Andang Arif Wibawa Dyah Susilowati Dyan Putri Raharjati Elvira Elvira Ikkasari Iloh Antarini Kartinah Wiryosoenjoyo Lila Fadilla Magdalena Sutanto Mardiyono Mardiyono Mardiyono Masita, Tutus Elsa Nur Hidayati RAHAJENG PUTRININGRUM Rahmat Budi Nugroho, Rahmat Budi Rinda Binugraheni, Rinda Rizal Maarif Rukmana Rizal Maarif Rukmana Siti Nur Arsih Sri Lestari Sunarsih Widyastuti Yolanda Kharisma Setia Yusup Subagio Sutanto Yusup Subagio Sutanto

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