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All Journal Jurnal Florea Journal of Health (JoH) Jurnal SainHealth SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Klinikal Sains (Jurnal Analis Kesehatan) JURNAL ANALIS LABORATORIUM MEDIK Jurnal Kesehatan Poltekkes Kemenkes RI Pangkalpinang Jurnal Mitra Kesehatan ABSYARA: Jurnal Pengabdian Pada Masyarakat Binawan Student Journal Jurnal Sintesis: Penelitian Sains, Terapan dan Analisisnya Jurnal Pengabdian Masyarakat Bhinneka MANDALIKA VETERINARY JOURNAL Prosiding Seminar Nasional Pemberdayaan Masyarakat (SENDAMAS)
Intan Kurniawati Pramitaningrum
Universitas Binawan
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Bacterial Contamination of Medical Masks Across Different Wear Durations in Campus Setting Wulan Fitriani Safari; Achmadi; Intan Kurniawati Pramitaningrum; Silvi Almadhita Saphira; Amar Rizqi Kusuma; Sheila Camelia
Florea : Jurnal Biologi dan Pembelajarannya Vol. 13 No. 2 (2026)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v13i2.24519

Abstract

Medical masks play an important role in minimizing infection risk; however, they may also act as reservoirs for bacterial contamination. Mask wear duration is a key factor influencing microbial accumulation. While previous studies have focused on healthcare, industrial, or community settings, limited attention has been given to campus environments, where prolonged mask use is common among students and staff. This study aimed to evaluate bacterial contamination on disposable medical masks across different wear durations in a campus setting. This study involved 10 student who wore disposable medical masks for 0 (control), 3, and 6 hours under campus conditions. Bacterial contamination was assessed using the Plate Count Agar (PCA) method following serial dilution, and results were expressed as CFU/cm². Data were analyzed using using the Kruskal–Wallis test. The results showed a progressive increase in bacterial contamination with longer mask wear duration, from 0 hours (0 ± 0 CFU/cm²), to 3 hours (34.8 ± 28.04 CFU/cm²), and sharply at 6 hours (7297.59 ± 9552.08 CFU/cm²). Significant differences were observed between wear durations. In conclusion, extended mask wear duration may increase the risk of bacterial accumulation; therefore, appropriate mask replacement practices are recommended to support personal hygiene and health protection
Co-Authors Achmadi Achmadi Achmadi Afifah, Nazwa Pramitya Amar Rizqi Kusuma Amelia, Ria Andi Harmawati Novriani HS Arie Kusumawati Ayu Trisna Dewi, Dewa Ayu, Olga Dewi Citra Bara, Steffany Nurjanah Batu Da Costa, Sandra Dian Rachma Wijayanti Dian Rachma Wijayanti Dwijastuti, Ni Made Sri Emhari, Awaliyah Enny Khotimah febrianti febrianti I GUSTI AGUNG AYU SATWIKHA DEWI - Inggraini, Maulin Lili Rosmalia Muhammad Rizki Kurniawan N.S Widada Ni Luh Putu Agustini, Ni Luh Putu Nicolaus Sri Widada Noor Adrya Ilsan Nurcholis Majid Nurhusna, Maryam Pradana, Anung Ahadi Purboningrum, Diah Ayu Retno Fania Ritonga, Ahmad Fitra Rosmalia, Lili Sabarina Elfrida Manik Sabda Lingga, Pudja Aditya Sari, Anita Fatikah Sari, Puspa Puspita Septiani Septiani Septiasari, Ni Putu Senshi Shafa Nabilah Sheila Camelia Silvi Almadhita Saphira Siti Nurfajriah Suhesti, Rika Tia Novita Vivi Zumrotin Wulan Fitriani Safari Yundari, Yundari