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Journal : Journal of Tropical Soils

Study of Root Exudate Organic Acids and Microbial Population in the Rhizosphere of Oil Palm Seedling . Anandyawati; Enok Sumarsih; Budi Nugroho; Rahayu Widyastuti
JOURNAL OF TROPICAL SOILS Vol 22, No 1: January 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i1.29-36

Abstract

Mutual interaction between plants and microbes occured in the rhizosphere is expected to increase productivity of crops or soil fertility for agriculture. Plants excrete root exudates to attract microbes, and then microbes obtain habitat and food supply from plants and can fulfill the nutrient requirements through assisted enzymatic activity.  The objective of the research was to study the types and amounts of root exudate organic acids, microbial population, and the relationship between root exudate organic acids and microbial population in the rhizosphere of oil palm seedlings. The study was conducted in a greenhouse using a planting medium of sterile quartz sand. The study was conducted using two factorials completely randomized design with three replications. The first factor was oil palm seedling age (control / no oil palm seed, 1, 3, 6, 9 and 12 months-old of oil palm seedlings) and the second factor was the periods of seedling growth (45, 90, 135 and 180 days), so in total there were 72 experimental units. The result of High Pressure Liquid Chromatography (HPLC) analysis revealed that four kinds of organic acids were observed in the rhizosphere of oil palm seedlings, with the highest concentration were: acetic acid (1.66 ppm), citric acid (0.157 ppm), malic acid (2.061 ppm) and oxalic acid (0.675) ppm. The highest total population of microbes, fungi, Azotobacter, phosphate solubilizing bacteria (PSB) and phosphate solubilizing fungi (PSF) were 19.38 × 106 cfu g-1 soil, 3.28 × 104 cfu g-1 soil, 12.09 × 105 cfu g-1 soil, 8.39 × 104 cfu g-1 soil and 1.15 × 104 cfu g-1 soil, respectively. There are positive correlations between root exudate organic acids and total microbes, fungi, Azotobacter, PSB and PSF are.Keywords: microbes, organic acids, rhizosphere, root exudates
The Effect of Soil Moisture Content and Animal Manure Application on the Growth of Mendong (Fimbristylis globulosa (Retz.) Kunt) Yaya Sunarya; Enok Sumarsih
JOURNAL OF TROPICAL SOILS Vol 22, No 2: May 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i2.107-112

Abstract

The objective of the research was to study the interaction between soil moisture content and animal manure application on the growth of Mendong (Fimbristylis globulosa (Retz.) Kunt). The experiment was conducted in May until September 2016 in Kampung Lembur Sawah, Kamulyan Village, Manonjaya Subdistrict, Tasikmalaya Regency. A pot experiment was performed using a factorial design consisting of two factors and three replicates. The factors were soil moisture content (K) consisting of four levels i.e. k1 (100% field capacity (FC)), k2 (150% FC), k3 (200% FC), and k4 (250% FC); and animal manure application (P) consisting of p1 (cattle manure), p2 (broiler chicken manure), and p3 (sheep manure). The indicators of plant growth, i.e. plant height, number of tiller, fresh weight and dry weight of plant biomass, shoot/root ratio, percentage of  the >75cm-long stems, water consumption, and water use efficiency (WUE) were measured.  The results showed that  there was an interaction effect between the soil moisture content and animal manure application on the number of tiller at 45 days after planting (DAP), the percentage of the >75 cm-long stems, and the water consumption. The sheep manure application at any levels of soil moisture content resulted in better effect on the growth of Mendong, water use efficiency, and quality of stem (the percentage of the >75cm-long stems). The soil moisture content above the field capacity resulted in better effect on the growth of Mendong, indicating that Mendong is more suitable to grow on the land with the soil moisture content above the field capacity or saturated soil during the whole growing period. Keywords: Manure, Mendong, soil moisture content