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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia AQUATIC SCIENCE & MANAGEMENT Media Teknologi Hasil Perikanan JURNAL ILMIAH PLATAX JURNAL PERIKANAN DAN KELAUTAN TROPIS Jurnal Ilmu dan Teknologi Pangan
Hens Onibala
Fakultas Perikanan Dan Ilmu Kelautan, Universitas Sam Ratulangi. Jl. Kampus Unsrat Bahu, Manado 95115, Sulawesi Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media

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Microbiologys Quality of Fish Sauce Tongkol (Euthynnus affinis) with Additions Pineapple Juice (Ananas comosus) Irandha Citra Marasi Siahaan; Henny Adeleida Dien; Hens Onibala
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 3 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia 20(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.371 KB) | DOI: 10.17844/jphpi.v20i3.19775

Abstract

Fish sauce is one of fish fishery product from fermentation process. In this study fish sauce madefrom Tongkol fish (Euthynnus affinis) that has high catching in North Sulawesi. This study aimed to makediversification product from Tongkol fish into fish sauce safe to eat. The research method applied wasexperimental laboratories. In this research was by additions (A) 0%, (B) 9%, (C) 12%, (D) 15% pineapplejuice and (G8) 8%, (G9) 9%, (G10) 10% salt and stored in an incubator at temperature 50ºC during 6,8, 10 days. Variable measured were Total Lactic Acid Bacteria, Total Plate Count, E.coli, Staphylococcussp, gram strain, pH, water consentration and organoleptic test. The result showed that sample D (D1G10)with additions pineapple juice 15% caused decrease total pathogen bacteria and caused increase lactic acidbacteria. Showed that Total Lactic Acid Bacteria, Total Plate Count, E.coli, Staphylococcus sp, gram strain,pH, water consentration, gram strain, organoleptic test with pineapple juice 15% was 1.0x102; 9.0x104; 5.46;69.14%; negative; 100 isolate positive shaped rod; the appearance 8; smell 8; taste 8. The result accordancewith Standard National Indonesia (SNI) and safe to eat.
DNA Isolation And Amplification of the rbcL (ribulose-1,5- bisphosphate carboxylase/oxygenase large subunit) gene of Caulerpa sp., Gracilaria sp., And Sargassum sp. Biondi Tampanguma; Grevo S. Gerung; Veibe Warouw; Billy Th Wagey; Stenly Wulllur; Deiske A. Sumilat; Hens Onibala
Jurnal Ilmiah PLATAX Vol. 8 No. 2 (2020): ISSUE JULY-DECEMBER 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.8.2.2020.30003

Abstract

DNA isolation and gene amplification of algae are significantly influenced by various factors such as characteristics and components of the algae cell wall. Therefore techniques and methods of DNA isolation in certain algae, sometimes only succeed in one particular species and can not be applied to another algae species. Based on that issue, this study was conducted with the aims to determine the succeed of DNA isolation and amplify the rbcL gene as a target gene for identification. Algae DNA was isolated by using innuPrep Plant DNA commercial kit, and the second one with a modified conventional Cetyl Trimetyl Ammonium Bromide (CTAB) method,  for the amplification process was using rbcL gene (ribulose-1,5-bisphosphate carboxylase / oxygenase large subunit) with two pairs of primers : rbcL 7F-753R and rbcL 577F-rbcSR. The results showed that the DNA of Gracilaria sp was succeed isolated by using CTAB method and it was denoted by the presence of DNA bands in agarose gel. Meanwhile DNA amplification for Gracilaria sp., and Sargassum sp., were succeed amplified with the appearance of DNA bands. But in algae Caulerpa sp., was only succeed on 1 pair of primary rbcL 7F and 7.Keywords : DNA, gene rbcL, algae Caulerpa sp., Sargassum sp., Gracilaria sp;AbstrakIsolasi DNA dan amplifikasi gen pada alga sangat dipengaruhi oleh beberapa faktor seperti karakter dan komponen pada dinding sel alga. Oleh karena itu proses isolasi DNA terkadang bisa berhasil pada satu jenis alga, namun tidak berhasil pada jenis alga lainnya. Oleh karena alasan tersebut, maka penelitian ini dilakukan untuk menentukan keberhasilan Isolasi DNA dan mengamplifikasi gen rbcL sebagai gen target identifikasi. Penelitian ini dilakukan dengan tahapan awal Isolasi DNA yang menggunakan kit komersil innuPrep Plant DNA Kit, dan metode konvensional Cetyl Trimetyl Ammonium Bromide (CTAB) yang telah dimodifikasi. Sedangkan untuk proses amplifikasi, menggunakan gen rbcL (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit) digunakan dua pasang primer yaitu rbcL 7F-753R dan rbcL 577F-rbcSR. Hasil isolasi DNA dari alga Gracilaria sp berhasil diisolasi menggunakan metode CTAB yang ditandai dengan adanya pita DNA pada gel agarose. Amplifikasi DNA pada alga Gracilaria sp., dan Sargassum sp., berhasil diamplifikasi dengan munculnya pita DNA. Namun pada alga Caulerpa sp. hanya berhasil pada 1 pasang primer rbcL 7F dan753R.Kata kunci : DNA, gen rbcL, alga Caulerpa sp., Sargassum sp., Gracilaria sp.
Quality Characteristics and Shelf Life of Smoked Skipjack Fish (Katsuwonus pelamis L) Paputungan, Mohammad Reza; Onibala, Hens; Harikedua, Silvana D.; Salindeho, Netty; Mongi, Eunike L.; Mentang, Feny; Tappy, Miranto Stevanus
JURNAL PERIKANAN DAN KELAUTAN TROPIS Vol. 12 No. 3 (2023): EDISI ESPTEMBER-DESEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jpkt.v12i3.51737

Abstract

One of the typical fishery products of North Sulawesi is whole smoked skipjack which is still traditionally processed. The purpose of this study was to determine the quality of whole smoked skipjack during storage at room temperature in terms of moisture content, total plate count, total mold, and organoleptic value. The results showed that the organoleptic value, moisture content, total bacteria, and total mold only met the Indonesian National Standard on day 0 of storage and exceeded the limit on day 3 of storage. Keywords: Smoked Skipjack tuna; Moisture content; Total Plate Count; Mold Abstrak Salah satu produk perikanan khas Sulawesi Utara adalah ikan cakalang asap utuh yang masih diolah secara tradisional. Tujuan dari penelitian ini untuk mengetahui mutu ikan cakalang asap utuh selama penyimpanan pada suhu ruang dilihat dari kadar air, angka lempeng total, total kapang dan nilai organoleptik. Hasil penelitian menunjukkan bahwa nilai organoleptik, kadar air, total bakteri dan total kapang hanya memenuhi Standar Nasional Indonesia pada penyimpanan 0 hari dan sudah melewati batas pada hari ke-3 penyimpanan. Kata kunci: Cakalang asap; Mutu; Kadar air; Angka Lempeng Total; Kapang
Molecular Identification And Conservation Status Of Sharks From The Fins Trade In Manado City North Sulawesi Mopay, Maratade; Wullur, Stenly -; Onibala, Hens -; Ginting, Elvy Like; Rumengan, Inneke F. M; Sondak, Calvyn F.A; Sumilat, Deiske A.
Jurnal Ilmiah Platax Vol. 9 No. 2 (2021): ISSUE JULY-DECEMBER 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v9i2.36016

Abstract

Sharks are a group of cartilaginous fish that are vulnerable to overfishing.  Genetics approaches play an important role in shark conservation. Shark fishing has become the main activity of fishermen in several areas, including in North Sulawesi. This research is focused on the molecular aspects and conservation status of shark species obtained from the shark fin trade in Manado, North Sulawesi. COI gene was amplified using Fish BCL5 (for) and HCO219 (rev) primers. Nucleotide sequences of each sample were aligned with the closest sequences in the GenBank database using the BLAST (Basic Local Alignment and Search Tool) method. The conservation status of the shark species is carried through the IUCN (International Union for the conservation of nature red list) Red list website. Molecular identification results showed that the shark’s fins from Manado had high similarity with Carcharhinus falciformis (HM1 and HM2) and Carcharhinus melanopterus (HM3). According to IUCN red list data, the C. falciformis and C. melanopterus were categorized as vulnerable to extinction (VU).Keywords:  Molekuler; COI; Shark; Manado and IUCN Red list . AbstrakHiu merupakan kelompok ikan bertulang rawan yang sangat rentan terhadap dampak penangkapan secara berlebihan.  Informasi terkait genetik hiu  yang semakin terancam populasinya sangat berperan penting dalam upaya konservasi hiu. Penangkapan hiu telah menjadi aktivitas utama nelayan di beberapa daerah, termasuk di Sulawesi Utara. Penelitian ini difokuskan pada aspek  molekuler dan penentuan status konservasi spesies hiu menggunakan sampel sirip yang di dapatkan  dari perdagangan sirip  hiu, di kota Manado.  Amplifikasi gen COI dilakukan dengan menggunakan primer Fish BCL5 (for) dan HCO219 (rev). Sekuens nukleotida masing-masing sampel disejajarkan dengan nukleotida terdekat yang ada dalam database genbank menggunakan metode BLAST (Basic Local Aligment and Search Tool)  Penentuan status konservasi dilakukan melalui penelusuran spesies rujukan di situs IUCN (International Union for the Conservation of Nature) Red list. Hasil identifikasi molekuler menunjukan bahwa sampel sirip hiu dari  kota Manado  memiliki kemiripan yang tinggi dengan spesies : Carcharhinus falciformis  (HM1 dan HM2) dan C. melanopterus (HM3).  Menurut data IUCN Red list, C. falciformis dan C. Melanopterus merupakan jenis hiu dalam status konservasi rentan punah (VU). Kata Kunci: Molekuler; COI; hiu; Manado dan IUCN Red list
Study of Planned Maintenance System Senior Admiral John Lie’s Training Ship on Shipping Safety Ansar, Azhar Ariansyah; Luasunaung, Alfret; Sumilat, Deiske Adeliene; Pamikiran, Revols Dolfi Ch; Kaparang , Frangky Erens; Onibala, Hens
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.48973

Abstract

The implementation of the ship maintenance management system on Rear Admiral Jhon Lie's training ship did not run optimally, which impacted the less-than-optimal condition of various training ship equipment because the crew did not carry out the ship maintenance system according to the procedure, resulting in the incident that the ship's anchor winch operation did not function optimally when the ship was anchored in Belang Port, Southeast Minahasa. This study aims to identify and analyze the positive and significant effect of the planned maintenance system on shipping safety. The primary data collection methods used in this study were questionnaires and interviews. In this study, data processing and presentation were in the form of diagrams and path analysis with structural equation modeling (SEM-PLS) using SmartPLS. From the results of the data analysis, the R-square value of shipping safety (Y) is 0.307, which means that the planned maintenance system (X1) and ship maintenance (X2) can affect shipping safety (Y) by 30.7%. Ship maintenance (X2) has a significant effect on sailing safety (Y), with P-values = 0.033 0.05 (hypothesis accepted). The planned maintenance system (X1) has a significant effect on sailing safety (Y), with a P-value of 0.037 0.05 (hypothesis accepted). As a result of the analysis, it was found that the planned maintenance system has a significant effect on shipping safety.
Co-Authors Adel Tuyu Adnan Wantasen Agnes T. Agustin, Agnes T. Agnes Triasih Agustin Alfianto, Fauzan Alfret Luasunaung Angela, Gabriella Caristy Ansar, Azhar Ariansyah Anti, Richard Suhartono Bentalen, Septian Gedoan Billy Th Wagey Biondi Tampanguma Calvyn F. A. Sondak, Calvyn F. A. Daisy Monica Makapedua Damopolii, Niki Sirwanto Deiske A. Sumilat Deiske Adeliene Sumilat, Deiske Adeliene Dewi Sahnita Elieser Imbir Engel Victor Pandey, Engel Victor Feny Mentang Frans G Ijong Frans G. Ijong, Frans G. Frans Ijong Ginting, Elvy Like Gordianus P Lombongadil Grace Sanger, Grace Grevo S. Gerung Gusu, Jahara Henny A Dien Henny Adeleida Dien I K Suwetja I Ketut Suwetja Imbir, Yakobus Kostan Inneke F. M Rumengan Irandha Citra Marasi Siahaan J Aquarista Ingratubun Jenki Pongoh Joshian N.W. Schaduw Joyce CV Palenewen Kaparang , Frangky Erens Kaseger, Bertie Kaseger, Bertie Elias Lohoo, Helen Jenny Makapedua, Daisy Makapedua, Daisy M Massie, Tita Mentang, Fenny Mewengkang, Hanny Mongi, Eunike L. Mopay, Maratade Nity, Elroi Nurmeilita Taher Pamikiran, Revols Dolfi Ch Pandey, Engel Paputungan, Mohammad Reza Putra, Debriga Reo, Albert Royke Roike Iwan Montolalu Ruslan Daeng Matteru, Ruslan Daeng Saliada, Farnis Salindeho, Netty Samuel Jeujanan, Samuel Sandria Stephanie Pundoko Selfia M. Nara Sembiring, Tuhu Edi Suranta Siegfried Berhimpon Silvana Dinaintang Harikedua Simbolon, Sonia Epriana Singkuku, Febri Triani Stenly Wulllur Stenly Wullur Suria Darwisito, Suria Tangkaa, Raymundus Tappy, Miranto Stevanus Veibe Warouw Verly Dotulong, Verly Wowiling, Fernando Youce M Bintang