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Journal : journal of bio-molecule research and engineering

Hydrolytic Enzyme Producing Bacteria From The Gut Of Oryctes rhinoceros Larvae Inhabiting Household Waste Composer Ana Mariatul Khiftiyah; Ni'matuzahroh; Mochammad Affandi; Salamun; Silvia Kurnia Sari; Miftahul Jannah; Nastiti Trikurniadewi; Achmad Zainal Abidin; Nurul Wahyuni; Rizki Amaliah Zain; Dela Dwi Alawiyah; Brigita Nur Diyan Agustiana
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.67564

Abstract

relatively shorter adult phase than the larval stage. The larval stage of this organism lives in piles of organic matter, including in piles of household waste. The ability of larvae to utilize organic matter in their environment is inseparable from the role of potential microbes that produce enzymes associated with their gut. This study aims to isolate, screen, and identify the potential bacteria in producing hydrolytic enzymes from gut of O. rhinoceros larvae. The third instar larvae were dissected aseptically to obtain gut bacterial isolates. The isolates were tested for their ability to produce amylase, cellulose, lipase, and protease using agar plate containing specific substrates. There were eight different isolates, and three of them had the ability to produce all four enzymes. The three isolates were identified as Bacillus. These results can be used as a reference for handling household organic waste.
Pycnoscelus surinamensis Endosymbiont Bacteria: Identification and its Amylase Characteristic Ni'matuzahroh; Fatimah; Sholikah; Ayu Tri Wahyuni; Tri Nurhariyati; Mochammad Affandi; Agus Supriyanto; Almando Geraldi; Tini Surtiningsih; Brigita Nur Diyan Agustiana; Dela Dwi Alawiyah; Ana Mariatul Khiftiyah; Silvia Kurnia Sari
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.67571

Abstract

The amylase is an enzyme that widely used in industry. In this research, amylase was isolated from endosymbiont bacteria (EKA 4) of cockroach Pycnoscelus surinamensis from household garbage dump Surabaya. This research aims to identify bacteria EKA 4, produce and characterize amylase from it at various temperature, pH and effect of metal ions. Amylase screening assay was done by imoculating the isolate into medium contain 2% of starch then incubated for 24 hours. Detection of amylase was done qualitatively by measured presence of halozone and quantitatively using DNS method. 16S rRNA analysis found that bacteria EKA 4 has similarity to Bacillus subtilis with 100% of percent identity. Result showed that optimum activity of amylase from B. subtilis were at 40°C, pH 6 and could use ion CaCl2 as a cofactor in a reaction. Thus, utilization of amylase from endosymbiont P. surinamensis could be as an alternative source in big industries.
Co-Authors Abdul Jabar ACHMAD ZAINAL ABIDIN Affandi, Moch AGUS SUPRIYANTO AGUS SUPRIYANTO Agus Suhadi Agus Suhadi Ahmad Saiful Arifin Ahmad Subro AISYAH RAHMAWATI ALMANDO GERALDI Almando Geraldi Amrini Shofiani Anggi Indah Yuliana Anggik Ardianto Ardy Ansyah ARSY HABI PRIYATAMA Arsy Habi Priyatama Ayu Tri Wahyuni BERLIAN RUSTANTINA BRIGITA NUR DIYAN AGUSTIANA Brigita Nur Diyan Agustiana Canggih Nailil Maghfiroh Canggih Nailil Maghfiroh CHRISTOPHER CLEMENT Chumaidi, Mochamad DAMAN HURI DELA DWI ALAWIYAH Dela Dwi Alawiyah Dian Irma Suryani Dyah Ayu Sri Hartanti ELVIS PERDANA PUTRA Fatimah FATIMAH FATIMAH Hanik Lutfiyah HESTI NURHAYATI JOHAN SETIAWAN Junairiah Junairiah KIRANA ATMANINDYA LAILY AINUN JAIYAH Lisa Lisdiana M. Jalalluddin Al Kafi Maghfiroh, Canggih Nailil Miftachul Chusnah MIFTAHUL JANNAH MOCH. AFFANDI MOCH. AFFANDI Mochammad Iam Fahmition Mohamad Mahfud Baydlowi Mohamad Nasiruddin Mohamad Nasiruidin Mohammad Roikhul Fatahillah Muhammad Muzadib Muhammad Zainul Nasirudin, Mohamad Nastiti Trikurniadewi Nerisa Agnesia Widianto Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh NI’MATUZAHROH NI’MATUZAHROH NI’MATUZAHROH NI’MATUZAHROH Nurul 'Aini Nurul Aini NURUL WAHYUNI Nurul ‘Aini Rahmah Cahya Ningrum RICO RAMADHAN Rizki Amaliah Zain Rizqi Nurul Huda SALAMUN SALAMUN SALAMUN Sholikah SILVIA KURNIA SARI SILVIA KURNIA SARI Silvia Kurnia Sari Silvia Kurnia Sari Siti Aminatuz Zuhria Siti Nur Qomariyah Thohirin Thohirin Tini Surtiningsih TRI NURHARIYATI Tri Nurhariyati Wika Ifananda Yessita Puspaningrum Yuliani Yuliani