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All Journal Natural Science: Journal of Science and Technology METAMORFOSA Journal of Biological Sciences Microbiology Indonesia Pendidikan Ekonomi Pendidikan Biologi Jurnal Biologi Universitas Andalas Jurnal Riset Kimia agriTECH Bioeksperimen: Jurnal Penelitian Biologi Jurnal Litbang Industri Biopropal Industri Jurnal Biologi Tropis Jurnal Hilirisasi IPTEKS (JHI) Buletin Ilmiah Nagari Membangun Bioscientist : Jurnal Ilmiah Biologi Jurnal Litbang Industri
Periadnadi Periadnadi
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Andalas, Limau Manis, Padang, Sumatera Barat 25175
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Potensi Antimikroba dan Antioksidan Beberapa Ekstrak Daun Benalu (Scurrula ferruginea (Roxb. ex Jack) Danser dari Archidendron sp. terhadap Staphylococcus aureus, Escherichia coli, dan Candida albicans Annisa Apriyelita; Periadnadi Periadnadi; Nurmiati Nurmiati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11108

Abstract

Scurrula ferruginea (Roxb. ex Jack) Danser is known for its role as an antimicrobial and antioxidant agent due to its content of phenolic compounds, alkaloids, and terpenoids. S. ferruginea has been widely used in traditional medicine for skin infections, diarrhea, hypertension, and digestive tract diseases. This study aims to determine the antimicrobial and antioxidant potential of various treatments of S. ferruginea mistletoe extracts. The experimental method used in this research is the nested pattern experiment. The extract treatments applied include fresh extract, boiled dry extract, boiled fresh extract, brewed dry extract, and brewed fresh extract. Antimicrobial potential was assessed using disc diffusion and dilution methods. Antioxidant activity (IC50) was determined using the DPPH (1,1-Diphenyl-2-Picryl-Hydrazine) method, and Total Phenolic Content (TPC) was also determined. The results indicate that the highest antimicrobial activity against Escherichia coli and Staphylococcus aureus was found in the strong category of fresh extract treatment, whereas there was no significant effect on Candida albicans growth. The fresh extract treatment exhibited the highest antimicrobial activity with a total phenolic content of 20.77321 mgGAE/mL. The Minimum Inhibitory Concentration (MIC) values for the fresh extract against S. aureus and E. coli were 6.25%, and the Minimum Bactericidal Concentration (MBC) values were 12.5% against both bacteria. The antioxidant value of the fresh extract was 101.26 μg/mL, categorized as moderate. Based on the conducted research, it can be concluded that S. ferruginea leaf extract can inhibit the activities of S. aureus and E. coli, while showing no effect on Candida albicans growth.
Eksplorasi Bakteri-bakteri Pemfermentasi dalam Beberapa Produk Tempe di Kota Padang Nurmiati Nurmiati; Periadnadi Periadnadi; Sherly Jadespi; Annisa Apriyelita
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11094

Abstract

Tempeh is one of Indonesia's traditional foods produced through the microbial fermentation process of Rhizopus. It has become a favorite among the community, typically cooked before consumption. The fermentative bacteria in tempeh are suspected to act as probiotics, potentially making tempeh a functional food. Thus far, there have been no reports on the presence of fermentative bacteria in soybean tempeh in Padang City. Therefore, this study aimed to analyze the presence of fermentative bacteria and enzymatic activities in various tempeh products in Padang City. The research focused on identifying the presence and proportion of fermentative bacteria in soybean tempeh in Padang City. The study utilized a survey method, and the data obtained were analyzed descriptively. The results indicated the total presence of bacteria in soybean tempeh samples (11.2 - 15.3 x 10^6 CFU/g), fermentative bacteria (3.30 - 6.10 x 10^6 CFU/g), acetic acid bacteria (0.90 - 1.50 x 10^5 CFU/g), proteolytic bacteria (2.10 - 3.70 x 10^6 CFU/g), cellulolytic bacteria (34.60 - 6.50 x 10^6 CFU/g), and amylolytic bacteria (2.20 - 4.90 x 10^6 CFU/g).
Eksplorasi Mikroflora Alami Cairan Nepenthes mirabilis (Lour) Druce di Kawasan Hutan Penelitian dan Pendidikan Biologi Universitas Andalas Hania Titami; Periadnadi Periadnadi; Nurmiati Nurmiati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11277

Abstract

Indonesia is a country that is rich in flora and fauna diversity, one of the unique flora in Indonesia is Nepenthes. This bag serves to trap insects or other small animals, the Biology Education and Research Forest (HPPB) is located in the Andalas Limau Manis University campus area which is classified as lowland tropical rain forest which is the habitat of Nepenthes, in the Biology Education and Research Forest (HPPB) this type of pitcher plant, namely Nepenthes mirabilis. Nepenthes mirabilis is a plant that is able to digest insects trapped in pockets at the ends of its leaf tendrils, so it is classified as a carnivorous plant. Microorganisms play an important role in breaking down organic materials into inorganic ones available to plants. Types of bacteria and bacterial populations greatly determine the decomposition process of animals that have been trapped in the bag as a source of nutrients for Nepenthes plants. This study aims to explore the natural microflora of Nepenthes mirabilis fluids, determine the proportional presence of potential bacteria, and determine the in vitro potential of chitinolytic bacteria from Nepenthes mirabilis fluids. This study used a survey method which was analyzed descriptively. The results showed that Nepenthes mirabilis fluid contained the presence of Fermentative, Amylolytic, Proteolytic, Chitinolytic and Lipolitical bacteria. Nepenthes mirabilis liquid in open bags contained chitinolytic bacteria, the highest presence of chitinolytic bacteria was found in MBB samples (20,104 cfu/ml) and MBA samples (18,104 cfu/ml). .28), fermentative (2.33 and 2.00), amylolytic (1.42 and 1.28), proteolytic (2.00 and 2.00), lipolytic (1.75 and 1.28).
Eksplorasi Mikroflora Alami Produk Fermentasi Tradisional Cangkuak Berbasis Daging di Kecamatan Kuantan Mudik Kabupaten Kuantan Singingi Adela Putri; Nurmiati Nurmiati; Periadnadi Periadnadi
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11216

Abstract

Cangkuak is a fermented food made from meat with betung bamboo shoots or kluwak, salt and rice. The fermentation process is carried out without adding microbial cultures (spontaneous) for one to three weeks in a tightly closed container. cangkuak's manufacturing technique, ingredient formulation and constituent ingredients are unique, resulting in a final product with different characteristics. Research on the presence of microbes in fermented food products (fish, milk, vegetables) has been widely conducted, but information on exploring the presence of natural microflora and the in vitro potential of fermenting bacteria in cangkuak has never been reported. This research was conducted using a survey method and the resulting data were presented descriptively. It was found that the natural microflora found in the three samples of cangkuak in Kuantan Mudik was dominated by bacteria, with the highest proportion of bacterial groups in the three cangkuak samples being fermentative bacteria (2.0 - 6.30x105 cfu/g), cellulolytic bacteria (3.30 - 5.90x105 cfu/g), amylolytic bacteria (1.70 - 4.40x105 cfu/g), proteolytic bacteria (0.12 - 7.90x105 cfu/g), lipolytic bacteria (0.08 - 0.12x105 cfu/g). Six potential fermentative isolates were obtained as evidenced by the calculation of index values, namely DK-I1 (1.59), DR-I1 (1.36), DR-I2 (2.49), DR-I3 (1.19), DKR-I1 (2.41), and DKR-I2 (2.96).
PENYULUHAN RUMAH PANGAN MANDIRI DI NAGARI SILANTAI, KECAMATAN SUMPUR KUDUS, KABUPATEN SIJUNJUNG, SUMATERA BARAT Efrizal Efrizal; Nurmiati Nurmiati; Periadnadi Periadnadi; Ando Fernando
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 1 No. 3 (2018)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/buletin ilmiah nagari membangun.v1i3.35

Abstract

The purpose of this extension activity was to share experiences, the application of science, self-potential extraction and problem solving that occurs in the community, especially in agriculture. The method used is survey and observation, counseling, demonstration plot and direct training in field. Based on the survey and primary data from Nagari Silantai, it can be seen that the Nagari located in Sumpur Kudus District is geographically in the position of 0o26'49 "LS and 100o54'29" BT, with an altitude of sea level around 243 m and rainfall of 300-4.000 mm/year. Judging from the topography, Nagari Silantai has a generally wavy and hilly land surface, with an area of ​​about 12.270 Ha. From the observations in the Nagari Silantai field consisting of 4 Jorong namely Jorong Kinkin, Jorong Koto Tangah, Jorong Koto Ateh and Jorong Ujung Koto. Judging from the data of the poor, Nagari Silantai is still classified as a poor economy community. This can be seen from the community poverty rate of 36.4%. Based on the potential of the area and the condition of the people in the Nagantai Silantai, we were encouraged to conduct independent food house extension activities in Nagari Silantai, Sumpur Kudus District, Sijunjung District, West Sumatra. From the results of this extension activity, it was seen that there was a public welcome and enthusiasm to increase knowledge both at the family level or community members' groups to develop food independence, improve the role and function of the Nagari community institutions and develop family awareness to be willing and motivated and able to use the land in its territory to be a source of food and nutrition.
Antimicrobial and Antioxidant Activity of Ants Nest Plant Extract (Hydnophytum formicarum Jack.) on The Growth of Test Microbials Nurmiati, Nurmiati; Periadnadi, Periadnadi; Putra, Wahyu Dwisa; Syafrina, Vellin Putri
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8700

Abstract

Ant nest plants are epiphytic plants that contain active compounds that act as antimicrobials. This study aims to determine the antimicrobial and antioxidant activities of ant nest plant extracts, determine the Minimum Inhibitory Concentration (MIC) and Maximum Killing Concentration (MBC) of test microbes, determine the presence of polyphenols from several ant nest extracts. The method used in this study is the nested pattern experiment method. The results showed that dry brew extract + lime gave a significantly different effect on dry brew extract and dry boil against E. coli and S aureus, but did not give a significantly different effect on C. albicans. The MIC of dry brewed extract + lime against E. coli, S.aureus, and C. albicans is 3.12%, 1.5% and 25%, and is able to kill with an MIC of 6.25%, 3.12% , and 50%. The polyphenol value in the dry brewed extract + lime was 11.28 mgGAE/mL, the dry brewed extract was 9.94 mgGAE/mL, and the dry boiled extract was 4.13 mgGAE/mL. The antioxidant value of dry brewed extract + lime with an IC50 value of 78.14 μg/ml, dry brewed extract with an IC50 value of 98.68 μg/ml in the strong activity category and dry boiled extract with an IC50 value of 106.24 μg/ml in the medium activity category.
Morphotype Analysis of Endophytic Fungus Isolated from Taxus sumatrana of Mount Singgalang, West Sumatra Region Sari, Wulan Komala; Periadnadi, Periadnadi; Nurmiati, Nurmiati
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7154

Abstract

Taxus sp. plants in temperate climates like Europe and America have been extensively studied and identified as promising sources of endophytic microbes that produce anticancer compounds such as Taxol. These endophytic microbes exhibit high adaptability to the specific growing conditions of their host plants. Research has highlighted metabolic differences between tropical and temperate endophytes. The presence of Taxus sumatrana plants in tropical areas like Mount Singgalang in West Sumatra, Indonesia was subjected to explore potential new sources of bioactive compounds from tropical microbes. This research aims to identify the morphotypes of endophytic fungi isolated from the barks of Taxus sumatrana plants originating from Mount Singgalang. The method used is small pieces of the inner bark of Taxus sumatrana were placed on the surface of growth medium (PDA). The small hyphae emerging from the piece of plant samples were regularly monitored for the morphological characteristics of the colony and conidia structure. Each fungal culture was checked for purity and transferred to another PDA plate by the hyphal tip method. The investigation isolated two endophytic fungi from the bark of Taxus sumatrana: Pestalotiopsis microspora and Neopestalotiopsis sp.
Antimicrobial Activity of Soaked and Boiled Jengkol Fruit Skin Extract against Staphylococcus aureus, Escherichia coli and Candida albicans Nurmiati, Nurmiati; Periadnadi, Periadnadi; Apriyelita, Annisa
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7193

Abstract

Jengkol (Archidendron jiringa) fruit skin contains saponin, tannin, flavonoid, and phenolic compounds which act as antimicrobials in inhibiting and killing microbial. This research aimed to analyze the differences in various jengkol fruit skin extracts in antimicrobial activity against test microbes, as well as to analyze the total phenolic content and total saponin content in each jengkol fruit skin extract. The research method was carried out using an experimental method. Factor A extract (soaking and boiling) and Factor B Microbe (Staphylococcus aureus, Escherichia coli, Candida albicans). The results of this research showed that extracts from soaking and boiling of jengkol fruit skin provided different inhibitory effects on the growth of S. aureus, E. coli, C. albicans. The best extraction method that produces the largest zone of inhibition for test microbes is boiling extract. The extract with the highest total phenolic content is boiling extract with a total phenolic content of 7.517 mgGAE/ml. The extract with the highest total saponin content is boiling extract with a total saponin content of 16.11%.
Potential and Optimization of Antibiotic Production Isolate Bacteria Antibiosis Bacillus sp. Origin of Salt Liquid Waste of Anchovy (Stolephorus Sp.) Against Bacteria Test Nurmiati, Nurmiati; Dwisa Putra, Wahyu; Periadnadi, Periadnadi
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7403

Abstract

Waste is one of the environmental problems that exists in every fish processing industry. Therefore, other ways are needed so that fish processing waste can be reused into something more useful. The use of waste that can be used is by using antagonistic microorganisms as antibiotic producing materials. The aim of this research is to determine the partial characteristics and potential of antibiosis bacteria and to obtain isolates that have antibiosis potential against S. aureus and E. coli. Determine the effect of the best substrate protein, the effect of the best incubation time, the effect of the best pH conditions, the effect of the best salinity levels, the effect of the best trace elements for Bacillus sp species isolates. in the production of antibiotics against the test bacteria S. aureus and E. coli. And determine the best optimization of salted anchovy water production media in the production of antibiotics against the test bacteria S. aureus and E. Coli. The research method was carried out by survey and the results were presented descriptively. The results of this research obtained isolates of Bacillus sp. circular in shape, overall edges, raised elevation, and white in color. Basil cell form, motile bacteria, and has a Potential Antibiosis Index value of 1.95; Proteolytic Index 1.67; Fermentative 1.36; Amylolytic 1.18; Cellulolytic 1.79; and Lipolitics 1.26. Optimum conditions for the antibiosis bacterial isolate Bacillus sp. in producing antibiotics, namely on skim milk protein substrate, incubation time 16 hours, pH 6.73; trace element Zn, Salinity Content 5‰. The optimum condition for isolates in salted anchovy water media to produce antibiotics is at a salt content of 100% (11 ppt).
Eksplorasi Bakteri-bakteri Pemfermentasi dalam Beberapa Produk Tempe di Kota Padang Nurmiati, Nurmiati; Periadnadi, Periadnadi; Jadespi, Sherly; Apriyelita, Annisa
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11094

Abstract

Tempeh is one of Indonesia's traditional foods produced through the microbial fermentation process of Rhizopus. It has become a favorite among the community, typically cooked before consumption. The fermentative bacteria in tempeh are suspected to act as probiotics, potentially making tempeh a functional food. Thus far, there have been no reports on the presence of fermentative bacteria in soybean tempeh in Padang City. Therefore, this study aimed to analyze the presence of fermentative bacteria and enzymatic activities in various tempeh products in Padang City. The research focused on identifying the presence and proportion of fermentative bacteria in soybean tempeh in Padang City. The study utilized a survey method, and the data obtained were analyzed descriptively. The results indicated the total presence of bacteria in soybean tempeh samples (11.2 - 15.3 x 10^6 CFU/g), fermentative bacteria (3.30 - 6.10 x 10^6 CFU/g), acetic acid bacteria (0.90 - 1.50 x 10^5 CFU/g), proteolytic bacteria (2.10 - 3.70 x 10^6 CFU/g), cellulolytic bacteria (34.60 - 6.50 x 10^6 CFU/g), and amylolytic bacteria (2.20 - 4.90 x 10^6 CFU/g).
Co-Authors Abdi Dharma Adela Putri Ando Fernando Annisa Apriyelita Ansofino Ansofino Anthoni Agustien Apriyelita, Annisa Awari Susanti Azizah Nurmalinda Dalli Yulio Saputra Dalli Yullio Saputra Deddi Prima Putra Desi Musliati Devi Syafriyani Diah Kharisma Sari Dina Wahyuna Dwisa Putra, Wahyu Efrizal Efrizal Eka Muliani Elisa Febriyanti Ervina Magdaulih Fauzan Azima Ferline Ariesta Fitri Wulandari Gustina Indriati Hafizatur Rahma Hania Titami Hezi Yolantika Ika Thalia Nissa Indra Anggriawan Intan Pramita, Intan Jadespi, Sherly K Kamsina Kamsina, K Kartika Indah Permata Sari Lara Annisa Lia Masefa, Lia Lusi Lusi Mades Fifendy Melisa Ainil Fajri Merla Pamericar Merry Tri Indah Permata Sari Mitra Angelia Mudinillah, Adam Nadia Merisya Nani Subandini Nasril Nasir Nasril Nasir Nilah Ratnasari, Nilah Nissa Arifa Novelina Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati, N Pebriana Nasution Pujiana Okta Putri Putra, Wahyu Dwisa Putri, Adela Rahmadani, Silmi Yusri Ramza Seswati Risca Adelina Atro Risya Saputri, Risya Roza Ika Putri RRP. Megahati Sari, Diah Kharisma Sari, Wulan Komala Sekatresna, Widiyanti Sherly Jadespi Silmi Yusri Ramadani Sri Indrayati Suci Pebra Jusana Swandi, Monica Kharisma Syafrina, Vellin Putri Syovia Osnita Tesri Maideliza Titami, Hania Tivani Ardini Uswatul Hasana Yulia M. Nur Yuni Sepriani Yuniar Yuniar YUSRA YUSRA Yusra Yusra Zozy Aneloi Noli