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All Journal Natural Science: Journal of Science and Technology METAMORFOSA Journal of Biological Sciences Microbiology Indonesia Pendidikan Ekonomi Pendidikan Biologi Jurnal Biologi Universitas Andalas Jurnal Riset Kimia agriTECH Bioeksperimen: Jurnal Penelitian Biologi Jurnal Litbang Industri Biopropal Industri Jurnal Biologi Tropis Jurnal Hilirisasi IPTEKS (JHI) Buletin Ilmiah Nagari Membangun Bioscientist : Jurnal Ilmiah Biologi Jurnal Litbang Industri
Periadnadi Periadnadi
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Andalas, Limau Manis, Padang, Sumatera Barat 25175
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ISOLASI DAN KARAKTERISASI BAKTERI ALAMI PENCERNAAN IKAN PATIN SIAM (Pangasius hypophthalmus ) SEBAGAI KANDIDAT PROBIOTIK Awari Susanti; Periadnadi Periadnadi; Nurmiati Nurmiati
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p17

Abstract

Penelitian tentang Isolasi dan Karakterisasi Bakteri Alami Pencernaan Ikan Patin Siam (Pangasius hypophthalmus) Sebagai Kandidat Probiotik di laksanakan di Laboratorium Mikrobiologi, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, dari bulan Oktober 2015 sampai Mei 2016. Penelitian ini dilakukan dengan metoda ekperimen terhadap karakter mikroflora alami pencernaan ikan patin yang di analisis secara deskriftif. Bakteri indigenous di isolasi dengan medium GPA + CaCo3, isolat yang didapat di tentukan karakter dan potensi nya. Hasil Penelitian menunjukkan bahwa didalam usus Ikan Patin Siam pada medium GPA + CaCo3 ditemukan 32 x 106 cfu/g bakteri indigenous yang bersifat fermentatif, yang dapat mengubah gula menjadi asam. Keempat isolat yang didapatkan memiliki karakteristik garam positif dan gram negatif. Berbentuk batang, dengan bentuk koloni yang berbeda-beda, katalase yang bereaksi positif dan negatif serta motilitas yang positif. Pada isolat PIRI3 memiliki nilai indeks degradatif tertinggi 1,6 dan tergolong dalam bakteri asam laktat pada genus Lactobacillus.
Keberadaan Mikroba Pemfermentasi pada Minuman Kefir Air Susu Kambing Etawa Merla Pamericar; Periadnadi Periadnadi; Nurmiati Nurmiati
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p15

Abstract

Study about existence of fermentation microbe in kefir etawa goat milk had been studied. The objective of this study were to know total of microbe from kefir etawa goat milk. This study used descriptive method and counting population of microbe on in vitro. The variable acid in kefir measuring with pH meter. Result indicated total bacteria in kefir etawa goat milk are sebanyak 98,3 x 106 cfu/ml with total yeast 67,8 x 105 cfu/ml and pH of kefir 4.57.
Aktivitas Antimikroba Ekstrak Segar Tumbuhan Sikaduduak (Melastoma malabathricum Linn.) Nissa Arifa; Periadnadi Periadnadi
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p05

Abstract

Penelitian tentang “Aktifitas Antimikroba Ekstrak Segar Tumbuhan Sikaduduak (Melastoma malabathricum Linn.)” menggunakan Rancangan Acak Lengkap dengan pola Nested. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba ekstrak segar buah masak, daun dan bunga Sikaduduak. Hasil penelitian menunjukkan bahwa ekstrak segar daun memiliki daya hambat terbesar terhadap Staphylococcus aureus dan Escherichia coli dengan rata-rata diameter zona hambat yaitu 17,33 mm dan 14,67 mm. Terhadap Candida albicans rata-rata diameter zona hambat terbesar (11,67 mm) terdapat pada buah masak.
PENGARUH DOSIS GULA DAN PENAMBAHAN EKSTRAK TEH HITAM TERHADAP FERMENTASI DAN PRODUKSI NATA DE COCO Lusi Lusi; Periadnadi Periadnadi; Nurmiati Nurmiati
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p19

Abstract

The purpose of this study was to look at the effect of sugar dose, black tea extract dose, interaction of sugar and black tea exstract dose on the fermentation and production of nata de coco. Completely Randomized Design (CRD) in Factorial was used in this experiment with sixteen treatments and two replications. The first factor was the doses of sugar and the second factor was doses of black tea extract. The results of this study showed that the doses of sugar and addition of black tea extract was able to increase the production of nata de coco. The best dose of sugar in 50 g/ l, the best dose of black tea extract in 8 g/ l. The interaction of sugar dose and black tea extract dose contained in the treatment a1b2 the dose of sugar 50 g/l and black tea extract 8 g/l with an average weight of 23,43 g nata de coco. The benefits of this research is to increase production and quality of nata for the nata de coco’s industry.
KECEPATAN PERTUMBUHAN KAPANG (Trichoderma harzianum Rifai A1300-F006) DAN AKTIVITAS SELULASE DALAM PENANGANANAN SAMPAH SELULOSA Pebriana Nasution; Periadnadi Periadnadi; Nurmiati Nurmiati
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p06

Abstract

Production of waste in urban areas reached 0.5 kg/person /day and 80% consisted of organic waste, one of many types of waste mostly generated is household waste. Generally, this household organic waste containing structural compoundsuch us long chains of cellulose.Therefore, the use of microorganism that can produce cellulase is very important to reduce the weight of garbage. Trichoderma harzianumis known as the most potential mold compared to other molds in converting cellulose. Sawdust and bran contain cellulose and hemicellulose that can be used as the main component in the mediafor its growth. The objectives of this study was: To find an effective ratio between sawdust and bran as growth mediafor Trichoderma harzianum, To know the cellulase activity of Trichoderma harzianum. This study has been conducted from April 2015 to Juli 2015 in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Andalas. The results of this study concluded that an effective ratio between sawdust and bran as a growth media for Trichoderma harzianumwhich degraded organic waste was 50:50. The highest activity of cellulase in degrading organic waste is 100%.
STUDI JAMUR POLYPORUS LIAR YANG BISA DIKONSUMSI (CENDAWAN ELANG) DI KECAMATAN KAYU ARO BARAT, KABUPATEN KERINCI, JAMBI Dalli Yulio Saputra; Nurmiati Nurmiati; Periadnadi Periadnadi
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p17

Abstract

Penelitian tentang studi jamur genera Polyporus liar (Cendawan Elang) yang bisa dikonsumsi telah dilaksanakan di Kecamatan Kayu Aro Barat, Kabupaten Kerinci dengan metode survei yang dianalisis secara deskriptif. Sampel dikoleksi secara purposive. Jamur liar konsumsi dengan nama lokal "Cendawan Elang" merupakan salah satu jamur dari genus Polyporus. Jamur ini hidup saprofit pada tumbuhan Ficus racemosa dan Alstonia scholaris yang telah mati dan lapuk dengan pH 4,78-5,03 pada suhu 16-28oC dan kelembaban udara 66-97% di ketinggian 1625-1853 mdpl.
PENGARUH PERTUMBUHAN MISELIUM BEBERAPA JENIS JAMUR TIRAM (Pleourotus spp.) MELALUI PENAMBAHAN KALSIT : DOLOMIT DALAM MEDIA SERBUK GERGAJI Hafizatur Rahma; Nurmiati Nurmiati; Deddi Prima Putra; Periadnadi Periadnadi
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p01

Abstract

Penelitian tentang “Pengaruh pertumbuhan miselium beberapa jenis jamur tiram (Pleourotus spp.) melalui penambahan kalsit : dolomit” dilakukan untuk mengetahui pengaruh penambahan kalsit : dolomit terhadap lama pertumbuhan miselium (vegetatif) beberapa jenis jamur tiram (Pleourotus spp.). Penelitian ini dilakukan di Laboratorium Mikologi, Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas. Penelitian dilakukan dalam Rancangan Acak Lengkap (RAL) pola nested dengan 2 kali ulangan. Faktor A berupa jenis jamur tiram (P. ostreatus, P. sajor-caju, P. flabellatus, P.cystidiosus) dan faktor B berupa variasi penambahan kalsit : dolomit (kontrol, 1:0, 1:2, 1:1, 1:2, 0:1). Hasil penelitian menunjukkan bahwa pertumbuhan miselium tercepat diperoleh dengan penambahan kalsit : dolomit 1:1 (19,62 hari) oleh P. flabellatus. Secara keseluruhan pemberian kalsit : dolomit dalam media serbuk gergaji berpengaruh terhadap kecepatan pertumbuhan miselium jamur tiram (Pleurotus spp.).
KECEPATAN PERTUMBUHAN DAN AKTIVITAS ENZIM LIGNIN PEROKSIDASE ISOLAT KAPANG LIGNOSELULOLITIK DALAM UPAYA PENANGGULANGAN SAMPAH ORGANIK LIGNOSELULOSA Elisa Febriyanti; Periadnadi Periadnadi; Nurmiati Nurmiati
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p12

Abstract

Research on the mycelial growth of mold isolates lignocelulolitic done using media substrate bran and sawdust. This research was conducted from April 2015 through March 2016 in the laboratory of Microbiology/ Mycology Department of Biology, Faculty of Mathematics and Natural Sciences, University of Andalas. This study aimed to compare the media effectively to the growth of mold isolates lignoselulolitik and lignin peroxidase (LiP) activity it generates in the response to the lignocellulosic organic waste. Comparison of media composition adapted to the treatment used is Bran 100%: Sawdust 0%; Bran 75%: Sawdust 25%; Bran 50%: Sawdust 50%; Bran 25%: Sawdust 75% and Bran 0%: Sawdust 100%. Parameters include the observation of mold growth on medium speed baglog and lignin peroxidase (LiP) activity was measured using a spectrophotometer at a wavelength of 310 nm with Veratryl Alcohol as a substrate. The results showed that the ratio of the bran media effectively : sawdust against mycelial growth of mold isolates are on treatment lignoselulolitik bran 75% : sawdust 25%, while the activity of lignin peroxidase (LiP) at the highest bran ratio of 50% : 50% sawdust.
ISOLASI DAN KEBERADAAN KHAMIR POTENSIAL PEMFERMENTASI NIRA AREN (Arenga pinnata Merr.) DARI DATARAN RENDAH DAN DATARAN TINGGI DI SUMATERA BARAT Periadnadi Periadnadi; Diah Kharisma Sari; Nurmiati Nurmiati
Bioeksperimen: Jurnal Penelitian Biologi Vol 4, No 1: March 2018
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v4i1.5927

Abstract

Penelitian bertujuan untuk mengisolasi dan mengkarakter khamir potensial pemfermentasi nira aren segar dari dataran rendah dan dataran tinggi di Sumatera Barat. Penelitian menggunakan metode eksperimen yang hasilnya disajikan secara deskriptif. Parameter yang diamati meliputi total mikroba, total khamir, uji potensi isolat khamir melalui uji fermentasi, karakter morfologi isolat khamir potensial pemfermentasi secara in vitro, serta analisis biokimiawi nira aren. Hasil penelitian menunjukkan bahwa keberadaan mikroba alami nira aren tertinggi terdapat pada sampel PAM1 (168 x 104 cfu/ml), total khamir tertinggi terdapat pada sampel HLB (85 x 104 cfu/ml), sedangkan presentase khamir tertinggi terdapat pada sampel HLB (69,1%). Uji potensi isolat melalui uji fermentasi diperoleh 5 isolat yang positifmemfermentasi alkohol. Berdasarkan karakter morfologi isolat-isolat khamir dari beberapa lokasi sampel secara in vitro didapatkan 3 isolat genus Hanseniaspora, 1 isolat genus Schizosaccharomyces, dan 1 isolat genus Saccharomyces.
Pengaruh jenis isolat-isolat bakteri fermentatif dari ubi kayu terhadap rendemen, derajat putih, dan bentuk granula tepung mocaf K Kamsina; N Nurmiati; P Periadnadi
Jurnal Litbang Industri Vol 9, No 2 (2019)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24960/jli.v9i2.5651.135-140

Abstract

Isolat bakteri indigenous ubi kayu merupakan isolat bakteri asli yang berada dalam umbi ubi kayu. Ubi kayu jenis lambau dan ketan (Manihot esculenta Crantz) merupakan jenis ubi kayu lokal Sumatera Barat, sedangkan ubi kayu kultivar karet (Manihot glaziovii)  merupakan ubi kayu dengan kandungan asam sianida (HCN) tinggi. Penelitian ini bertujuan untuk melihat kemampuan masing-masing isolat bakteri indigenous ubi kayu terhadap rendemen, derajat putih serta bentuk granula tepung mocaf yang dibandingkan dengan perlakuan tanpa pemberian isolat. Penelitian menggunakan rancangan acak lengkap (RAL) dengan perlakuan pemberian starter isolat bakteri indigenous ubi kayu jenis ketan (A), lambau (B), karet (C) dan tanpa isolat/kontrol (D). Terhadap produk mocaf  yang dihasilkan dilakukan pengujian meliputi rendemen, derajat putih, dan bentuk granula tepung mocaf. Hasil analisis terhadap berbagai perlakuan menunjukkan perlakuan optimal untuk tepung mocaf yang dihasilkan adalah pemberian starter isolat bakteri indigenous jenis ketan dengan rendemen  35,85%, derajat putih 94,40% dengan dinding sel ubi kayu sudah banyak yang pecah menjadi granula-granula yang lebih kecil dan memiliki rongga pada bagian granulanya.ABSTRACTCassava indigenous bacterial isolates are native bacterial isolates that are in tubers of cassava. Lambau and sticky rice (Manihot esculenta Crantz) are types of local cassava from West Sumatra, while rubber cultivar cassava (Manihot glaziovii) is a cassava with a high content of cyanide acid (HCN). This study was aimed to look at the ability of each indigenous cassava isolate to yield, the degree of white and the form of mocaf flour granules compared to the treatment without administration of isolates. The study used a completely randomized design (CRD) with the following treatments: administration of indigenous cassava isolate starter of sticky rice type (A), lambau (B), rubber (C) and without isolate / control (D). The mocaf products were tested including yields, white degrees, and the granules shape of mocaf flour. The analysis results of various treatments showed that the optimal treatment for mocaf flour produced was the provision of indigenous bacterial isolates starter from sticky rice type (A) with a yield of 35.85%,  white degree 94.40% with a lot of cassava cell walls that had broken into smaller granules and had cavities on the granules.
Co-Authors Abdi Dharma Adela Putri Ando Fernando Annisa Apriyelita Ansofino Ansofino Anthoni Agustien Apriyelita, Annisa Awari Susanti Azizah Nurmalinda Dalli Yulio Saputra Dalli Yullio Saputra Deddi Prima Putra Desi Musliati Devi Syafriyani Diah Kharisma Sari Dina Wahyuna Dwisa Putra, Wahyu Efrizal Efrizal Eka Muliani Elisa Febriyanti Ervina Magdaulih Fauzan Azima Ferline Ariesta Fitri Wulandari Gustina Indriati Hafizatur Rahma Hania Titami Hezi Yolantika Ika Thalia Nissa Indra Anggriawan Intan Pramita, Intan Jadespi, Sherly K Kamsina Kamsina, K Kartika Indah Permata Sari Lara Annisa Lia Masefa, Lia Lusi Lusi Mades Fifendy Melisa Ainil Fajri Merla Pamericar Merry Tri Indah Permata Sari Mitra Angelia Mudinillah, Adam Nadia Merisya Nani Subandini Nasril Nasir Nasril Nasir Nilah Ratnasari, Nilah Nissa Arifa Novelina Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati, N Pebriana Nasution Pujiana Okta Putri Putra, Wahyu Dwisa Putri, Adela Rahmadani, Silmi Yusri Ramza Seswati Risca Adelina Atro Risya Saputri, Risya Roza Ika Putri RRP. Megahati Sari, Diah Kharisma Sari, Wulan Komala Sekatresna, Widiyanti Sherly Jadespi Silmi Yusri Ramadani Sri Indrayati Suci Pebra Jusana Swandi, Monica Kharisma Syafrina, Vellin Putri Syovia Osnita Tesri Maideliza Titami, Hania Tivani Ardini Uswatul Hasana Yulia M. Nur Yuni Sepriani Yuniar Yuniar YUSRA YUSRA Yusra Yusra Zozy Aneloi Noli