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All Journal International Journal of Advances in Applied Sciences Jurnal Teknologi Dan Industri Pangan Jurnal Pangan dan Gizi JURNAL ILMU DAN TEKNOLOGI PETERNAKAN Animal Agricultural Journal Jurnal Pangan dan Agroindustri JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) agriTECH Jurnal Aplikasi Teknologi Pangan JMM (Jurnal Masyarakat Mandiri) Jurnal Aplikasi Teknologi Pangan Journal of Applied Food Technology Jurnal Teknologi Hasil Pertanian Jurnal Litbang Provinsi Jawa Tengah Jurnal Agro Dedikasi Masyarakat (JADM) Journal of Agritechnology and Food Processing Jurnal Teknologi Pangan Jurnal Profesi Insinyur Indonesia Jurnal Agripet
Yoyok Budi Pramono
Program Studi Teknologi Pangan, Fakultas Petenakan Dan Pertanian, Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Transportation Veterinary Other

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Journal : Jurnal Aplikasi Teknologi Pangan

 1 
Studi Kerusakan Protein dalam Emulsi Ganda Air-dalam-Minyak-dalam-Air Natrium Klorida Menggunakan Instrumen Fourier Transform Infrared Spectroscopy Yoyok Budi Pramono; Irene Raras Nawangsasi; Antonius Hintono Hintono; Vita Paramita Paramita
Jurnal Aplikasi Teknologi Pangan Vol 7, No 1 (2018): Februari 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3529.538 KB) | DOI: 10.17728/jatp.2130

Abstract

Penelitian ini bertujuan untuk mengetahui kerusakan protein dalam emulsi ganda W/O/W pada suhu penyimpanan (4, 25, 40°C) dan kadar NaCl yang berbeda (0; 0,2; 0,4; 0,6; 0,8; 1%) setelah 3 minggu penyimpanan dan dianalisis menggunakan instrumen FTIR. Protein lazim digunakan sebagai penstabil dan emulsifier alami dalam emulsi pangan, namun memiliki kelemahan dalam stabilitas terhadap suhu tinggi dan nilai pH rendah mendekati titik isolelektriknya. Penelitian ini menggunakan 2 jenis protein yaitu gelatin untuk fase air internal (W1) dan isolat protein kedelai (IPK) untuk fase air eksternal (W2). Emulsi ganda dibuat melalui tahap emulsifikasi ganda yaitu emulsifikasi primer untuk mendapatkan emulsi W/O dan emulsifikasi sekunder untuk mendapatkan emulsi ganda W/O/W. Analisis FTIR menunjukkan bahwa bahan baku yaitu gelatin dan isolat protein yang digunakan dalam pembuatan emulsi masih dalam kondisi baik. Interpretasi spektra protein yang digunakan dalam emulsi menunjukkan adanya penambahan dan hilangnya gugus Amida utama yang terkandung dalam gelatin dan isolat protein kedelai, yaitu Amida I, II, IV, V dan VI. Kesimpulannya, proses pemanasan emulsi pada suhu 4, 25, dan 40°C dan penyimpanan emulsi selama 3 minggu menyebabkan kerusakan struktur protein pada gelatin dan isolat protein kedelai yang tercermin melalui spektra protein melalui FTIR.This experiment aims to observe protein deterioration in food double emulsion W/O/W in various NaCl concentrations i.e. 0; 0.2; 0.4; 0.6; 0.8; 1% and storage temperatures, i.e. 4, 25, 40°C after 3 weeks storage by FTIR analysis. Protein is commonly used in food emulsion as stabilizer and natural emulsifier. However, protein has bad performance in high temperature and low pH value near to its isoelectric point. This experiment used 2 types of protein: gelatin as a protein that was added in internal water phase (W1) and soy protein isolate as a protein that was added in external water phase (W2). Double emulsions were produced by 2 phase emulsification, which were primary emulsification (to generate primary W/O emulsion) and secondary emulsification (to produce double emulsion W/O/W). FTIR analysis showed that gelatin and soy protein isolate as main ingredients for W/O/W double emulsion were in a good condition. Protein spectra interpretation showed that emersion and loss of main Amide group of gelatin and soy protein isolate in double emulsion, which were Amide I, II, IV, V and VI. It was concluded that heating and storing process caused protein structure damage to gelatin and soy protein isolate that was able to be visualized by FTIR analysis.
Co-Authors Abduh, Setya Budi M Adinur Chrismanuel Aghnia Ulul Azmi Putri Ahmad Ni’matullah Al–Baarri Al-Baarri, Ahmad Ni'matullah Al-baarri, Ahmad Ni-matullah Albaari, Ahmad N Anang M Legowo Anggun Novita Sari Antonius Hintono Antonius Hintono Argandhina Purbasari Arganis, Lusida Mulia Arini, Annisa Shafa Putri Arissaputra, Hermawan Astuti, Ayu Tri Ayu Anesakirani Ayu Ning Jagat Bambang Dwiloka Bhakti Etza Setiani Bintoro, V Priyo Bintoro, V. Priyo Bistyari, Ayunda Intan Clory Agustin Pienarso Daeli, Yohana Feynendra Dea Erik Bahtiar Dwi Agustina Wijayanti Gilang Ade Perdana Handoko, Nisfa Rama Kamila Hendri Hermawan Adinugraha Heni Rizqiati Hermawan Arissaputra Hermin Werdiningsih Hifni Sofyan Arief Irene Raras Nawangsasi Irene Raras Nawangsasi ITA PUSPITASARI Ita Puspitasari Kamil, Rafli Zulfa Khusania, Mina Lestari, Tiara Yunistha Listya, Angelique Gangsar Maharani, Arnetta D Masykuri Masykuri Melinda Erdya Krismaputri Muhammad Alwan Mahbub Muhammad As'ad Wibisono Muhammad Zulfahmi Nabila, Aulia Zalfa Narastyawan, Anggoro Rizky Nietha Yuliana Novia Tri Damayanti Nur Azizah Nur Hanifah Nurussyifa, Salsabila Yasinta Nurwantoro . Nurwantoro Nurwantoro Perdana, Gilang Ade Puspita Ayu Octaviani Puspita, Diyan Ayu Putri, Deshinta Ramadhani Bastian Putri, Sayyidah Ghaisani Putri, Yuni Maharani Rahim Fajar Pangestu Rakita Dewi Mega Ramadhaningrum, Ilma Muliasari Risa Fazriyati Siregar Robby Rusdiansyah Robby Rusdiansyah Safitri, Zevira Aldilata Selma Noor Permadi Septiawati, Tria Setya Budi M Abduh Setya Budi Muhammad Abduh, Setya Budi Muhammad Sigit Sulistiarto Sjafani, Nur Sri Mulyani Sudjono, Elisabeth Febriane Lovita Sulardjaka Sulardjaka Sulistiarto, Sigit Sulistiono, Fajar Budi Tomya, Stella Putri V Priyo Bintoro Valentinus Priyo Bintoro Vita Paramita Vitus Dwi Yunianto Budi Ismadi Wihardi Wihardi Wijayanti, Ana Rohana Nuranggraeni Yoga Pratama Yos Johan Utama Yulianto, Vitus Dwi Yusri Sapsuha Zanuarizky Oryza Ramadhani