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All Journal Dentika Dental Journal Majalah Kedokteran Gigi Indonesia Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Britain International of Humanities and Social Sciences (BIoHS) Journal Jurnal of Syiah Kuala Dentistry Society

Ameta Primasari

Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia

Author-ID : 347555

Religion Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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PERUBAHAN SEL EPITEL YANG TERJADI PADA PERMUKAAN MUKOSA BUKAL WANITA HAMIL Ameta Primasari; Cynthia Cynthia
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol. 12 No. 3 (2018): Jurnal Ilmiah PANNMED Periode Januari-April 2018
Publisher : Poltekkes Kemenkes Medan

Jaringan lunak rongga mulut dilindungi oleh mukosa yang merupakan lapisan epitel pada rongga mulut adalah stratified squamous epithelium. Stratified squamous epithelium terdiri atas epitel berkeratin dan epitel tidak berkeratin. Pada mukosa bukal terdapat sel epitel tidak berkeratin yang tersusun atas sel basal, sel intermediate dan sel superifisal. Selama masa kehamilan, peningkatan hormon estrogen 10 kali lipat dan progesteron 30 kali lipat. Perubahan hormonal ini mengakibatkan terjadinya proliferasi sel pada rongga mulut. Tujuan penelitian ini adalah untuk mengetahui perbedaan jumlah sel, ukuran diameter nukleus-sitoplasma, dan ratio nukleus sitoplasma pada sel epitel mukosa bukal wanita hamil dan wanita tidak hamil. Penelitian ini merupakan penelitian deskriptif analitik dengan rancangan cross sectional. Pengambilan sampel berupa sel epitel yang dilakukan terhadap 66 sampel sesuai dengan kriteria inklusi dan eksklusi. Setelah sel epitel didapat, langsung dibawa ke laboratorium Patologi Anatomi FK USU untuk dilakukan pewarnaan Haematoxilin-Eosin. Pemeriksaan mikroskopis untuk perhitungan jumlah rata-rata sel epitel, ukuran diameter nukleus-sitoplasma dan ratio nukleus sitoplasma yang dilihat dari 4 lapang pandang yang berbeda menggunakan mikroskop mikrograf. Hasil analisis uji Mann Whitney U menunjukkan terdapat perbedaan yang signifikan (p<0,05) pada jumlah sel epitel wanita hamil dan wanita tidak hamil. Sedangkan pada diameter nukleus-sitoplasma dan ratio nukleus sitoplasma menunjukkan tidak ada perbedaan yang signifikan (p>0,05) antara wanita hamil dan wanita tidak hamil. Berdasarkan penelitian ini dapat ditarik kesimpulan bahwa jumlah sel superfisial, ukuran diameter nukleus sel epitel mukosa oral dan ratio nukleus sitoplasma pada wanita hamil lebih besar dibandingkan wanita tidak hamil namun demikian ukuran sitoplasma pada wanita hamil lebih kecil dibandingkan wanita tidak hamil.

The level’s changing of transforming growth factor β2 during canine retraction in non-growing age patient Adianti Adianti; Ameta Primasari
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 2 (2015): June 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Orthodontic tooth movement occurred as a result of alveolar bone remodeling and collagen due to mechanical load. This mechanical load applied to the tooth will exert a number of cytokine and growth factors. One of the growth factors that are often associated with orthodontic tooth movement is transforming growth factor-β(TGF-β). It has 3 isoforms, TGF-β1, TGF-β2, and TGF-β3. It has been known that in adult patient, tooth movement rate was slower. Purpose: The aim of this study was to investigate the changing level of TGF-β2 in non-growing patient due to mechanical load in canine retraction. Method: Gingival crevicular fluid from 6 subjects who undergo canine retraction was taken to investigate changing level of TGF-β2. Distal site of each upper canine served as an experimental tooth. The gingival crevicular fluid from experimental tooth was taken just prior to mechanical load, at 24h and 72h after mechanical load. Result: ELISA reader showed that level of TGF-β2 was decreasing during experiment time. Conclusion: It can be concluded that in non-growing patient, TGF-β2 has less role in alveolar bone resorption in orthodontic tooth movement.

Senior High School Students References in Choosing Orthodontic Treatment Provider: A Study in Marelan Subdistrict 2018 Feni Firdanigrum; Kintoko Rochadi; Ameta Primasari
Britain International of Humanities and Social Sciences (BIoHS) Journal Vol 2 No 1 (2020): Britain International of Humanities and Social Sciences, February
Publisher : Britain International for Academic Research (BIAR) Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33258/biohs.v2i1.186

Initially the use of orthodontic appliance (braces) was intended to tidy up the teeth that had an irregular shape. The phenomenon of braces is increasingly prevalent among teenagers because in addition to health reasons then developed into a reason to support the appearance and eventually become a self-identity for adolescents. Finally, there are more victims of dental artisans who report disruption of oral health due to the installation of braces on dental artisans. Because the installation is not in accordance with the medical standard. The purpose of this study was to determine how senior high school student choose orthodontic treatment provider. To achieve these objectives, research was conducted using independent variables, namely knowledge, cost, distance, family support. The subjects of this study were 105 senior high school students. The subjects were taken using non-probability sampling and purposive sampling techniques. The data analysis method used is multiple linear regression test by taking into account the simultaneous test, classic assumption and partial test, correlation test determinant. The results of this study indicate that the variable cost (p=0, 0001), distance (p=0,014) and consumer knowledge (p=0,014) significantly influence the dependent variable (consumer decision) while the family support variable (p = 1,000) has no effect. Different from family (p = 1,000 > 0.05) support that has no relationship with consumer decision-making to choose orthodontic treatment provider, service costs (p = 0, 0001 < 0, 05), the distance traveled by respondents (p = 0.014 <0.05) and knowledge of respondents (p = 0.014 <0.05) have relationship with consumer decision making to choose orthodontic treatment provider both in Public and Private High Schools, and Public and Private Vocational Schools in Marelan Subdistrict, respectively.

Effect of aloe vera on the proliferation phase of oral mucosal wound healing in rats Primasari, Ameta; Yendriwati, Yendriwati; Minasari, Minasari; Buaya, Erlin Kristiani; Madani, Sri Ayu Winda; Sembiring, Kartika Nabila; Hidayati, Rizka
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77682

Aloe vera is known as a medicinal plant containing an active substance acemannan thought to play a role in accelerating wound healing. This study aims to determine the effect of aloe vera extract on the proliferation phase of oral mucosa wound healing in rats by looking at epithelial thickening, epithelial gaps closure, growth of new blood vessels, and connective tissue growth. This research is a pure experimental study with a post-test only control group design. On the left buccal mucosa of the mouth of 24 white Wistar rats (n = 24), an incision was made by scalpel with 10 mm length and 2.5 mm depth. The rats were divided into 4 groups: groups 1 and 2 as controls, while groups 3 and 4 were applied with aloe vera extract made by maceration technique. Wound tissue was observed histologically with Hematoxylin-Eosin staining under a microscope with an image analyzer for measuring the thickness of epithelium. The analysis of epithelial gap width used morphometry, and the number of capillaries was counted manually. Connective tissue thickness was measured with Image-J software. These were carried out on days 7 and 14 after treatment and the data were analyzed with the independent t-test and Mann-Whitney test. The results showed that aloe vera extract had no significant effect on epithelial thickening on day 7 (p = 0.701) nor on day 14 (p = 0.639), but a significant effect was shown on epithelial gaps closing (p = 0.049), connective tissue thickening (p = 0.004), and capillary increase on day 7 (p = 0.049). It could be concluded that aloe vera extract could potentially accelerate the epithelial gaps closure, connective tissue thickening, and capillary increase during the proliferation phase of the oral mucosa wound healing.

Potential Antibacterial of Binahong Leaf Extract Against Bacteria Enterococcus faecalis ATCCÂ® 29212â„¢ (in vitro): Potensi Antibakteri Ekstrak Daun Binahong Terhadap Bakteri Enterococcus faecalis ATCCÂ® 29212â„¢ (in vitro) Nasution, Minasari; Ameta Primasari; Ika Astrina; Siregar, Arfi Luthfiyah
Dentika: Dental Journal Vol. 25 No. 1 (2022): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/dentika.v25i1.7800

Â Binahong is a plant widely found in Indonesia and has been used for generations, but only by inheritance without much optimal research. Enterococcus faecalis is bacteria that cause many problems in the oral cavity such as periradicular lesion. The purpose of this study was to determine the zone of inhibition, Minimum Bactericidal Concentration (MBC), and Minimum Inhibitory Concentration (MIC) of Binahong leaf extract against bacteria Enterococcus faecalis ATCCÂ® 29212TM concentrations of 100%, 90%, 80%, 70%, and 60%, chlorhexidine 0,2% as positive control and DMSO as a negative control. The experimental method used for the research with a post-test only control group design, pure bacteria prepared in the microbiology laboratory, and Binahong leaf extract used in the chemical laboratory of the Faculty of Mathematics and Natural Sciences (FMIPA) at the University of North Sumatera. Five treatments of Binahong leaf extract concentration were tested as samples four times. MBC and MIC measurements of Binahong leaf extract against Enterococcus faecalis ATCCÂ® 29212TM were exchanged for each concentration. Mueller Hinton Agar (MHA) and Nutrient Broth incubated for 24 hours at 37Â°C. The ANOVA test data is analysed, followed by the Post Hoc test. The average inhibition zone of 100% concentration was 14.19 mm, 90% was 12.25 mm, 80% was (10.19 mm), 70% was 8.81 mm, 60% was 0.00 mm, chlorhexidine 0,2% was 20.88 mm, and DMSO was 0.00 m). The conclusion is that the best inhibition zone has a concentration of 100%, MBC was at 100%, and MIC was at 60%.

A Comparison of Yogurt and Probiotic Supplements Effects on the Hardness of Tooth Enamel: An In vitro Study: Perbandingan Pengaruh Yogurt dan Suplemen Probiotik terhadap Kekerasan Enamel Gigi: Studi in Vitro Primasari, Ameta; Alfarizi, Tengku Rizky; Minasari, Minasari; Yendriwati, Yendriwati; Hidayati, Rizka
Dentika: Dental Journal Vol. 25 No. 2 (2022): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/dentika.v25i2.8575

The demineralization of tooth enamel, which can be triggered by acidic foods and drinks at pH < 5.5, can initiate dental caries. Yogurt and probiotic supplements are drinks that are often consumed to help digestive function and improve the immune system. However, both drinks have a low pH, thereby, a long time of consumption can trigger the demineralization process. This study aims to evaluate the enamel hardness after applying yogurt and probiotic supplements, which was carried out experimentally in a laboratory with a one-group post-test design. The sample used is 9 human maxillary premolars in each treatment group. The treatment group comprises the test material, such as yogurt and probiotic supplements, as well as one control group with artificial saliva. Dental samples were immersed in the test material for 96 hours, and the enamel hardness level was measured with a Micro Vickers Hardness tester. The T-paired test showed that soaking with yogurt does not significantly affect the hardness of the enamel, as indicated by a p-value of 0.016. However, the effect was significant in probiotic supplements, with p=0.004. The results showed that soaking with yogurt was not significantly different from probiotic supplements in reducing enamel hardness, as evidenced by the Post Hoc (LSD) test (p=0.464). Conclusively, yogurt decreased enamel hardness less than probiotic supplements.

Effects of Sidamanik Black Tea on Salivary Flow Rate, Total Protein, and Albumin Levels in Young Adults: An in Vivo Study Astrina, Ika; Primasari, Ameta; Minasari
Dentika: Dental Journal Vol. 26 No. 2 (2023): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/dentika.v26i2.12717

Saliva is crucial in maintaining oral health, performing functions such as antibacterial activity, buffering, and ensuring tooth integrity. In this regard, black tea, a medicinal plant with potential anti-cariogenic agents, has been suggested to exert preventive effects against caries. Therefore, this study aimed to determine effects of black tea consumption on salivary flow rate, pH, as well as total protein and albumin levels in young adults. A pre-experimental design was used with a one-group pretest-posttest approach. The samples selected were 18 healthy young adults aged 18-25 years, with DMF-T scores of 1-5. Salivary flow rate, total protein, and salivary albumin were measured using a digital scale after a 5-minute interval, the bicinchoninic acid assay method, and the bromocresol green method, respectively. The results showed a significant increase in salivary flow, a substantial decrease in albumin, and an insignificant elevation in total protein. In conclusion, black tea manifested the capability to prevent caries by enhancing salivary flow and total protein levels. Furthermore, the impact in inhibiting bacteria activity depended on the presence of salivary albumin. Â

The sIgA and histatin-5 expressions in saliva of early childhood caries and caries-free children based on period times Dharli Syafriza; Heriandi Sutadi; Ameta Primasari; Yahwardiyah Siregar
Journal of Syiah Kuala Dentistry Society Vol 5, No 1 (2020): June 2020
Publisher : Dentistry Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jds.v5i1.18421

To analyze the level of sIgA and Histatin-5 expression in Early Childhood Caries (ECC) and caries-free children. Saliva was collected from 31 subjects of ECC and 29 caries-free (ages 9-32 months). The level expression of sIgA and Histatin 5 was analyzed by enzyme-linked immunosorbent assay. The distribution and frequency of subjects consist of boys 47% and girls 53% with the average of ages 1-12 month 21% and 13-24 month 79%.sIgA and Histatin 5 expressions differ from the month periods tested (1, 3, and 9 months) but do not show any significant difference from each examination period (1 with three months; p0.05: 0.116), (1 with nine months; p 0.05: 0.766), and (3 to 9 months; p0.05: 0.398). The expression of sIgA and Histatin 5 of boys and girls were not significant (p0.05:0.550) and weak correlation (r = 0.079).The level expression of sIgA in the Caries-free higher than ECC (p0.05). Whereas the expression of Histatin 5 in ECC higher than Caries-free (p0.05). The sIgA correlating in ECC and caries-free is significant (p0.05) and no correlation (r =- 0,29). Whereas Histatin 5 in the ECC and caries-free were strong correlation = 0,84. Both the result of ECC and caries-free suggested that the antibodies have been involved in the caries pathogenesis. In the ECC cases that occurred to decrease sIgA levels and increased of Histatin 5 levels, both antibodies have expressed in Caries-free Children. Both sIgA and Histatin 5 involved in the pathogenesis caries infection in children.KEYWORDS: Antibodies; Early Childhood Caries; saliva; secretory immunoglobulin A; Histatin-5

Differences in head size and shape during the growth of Deutero Malay children Primasari, Ameta; Angelia, Veronica; Agara, Dimas
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: The growth periods of 0-18 years old can be studied with anthropometry by identifying the head size and shape of the human head. Study on vertical, transverse, and horizontal cephalic indexes can understand differences and growth between sexes and age groups. This study was aimed to analyse the averages and differences in the head size and shape of Deutero Malay children in different age groups. Methods: This research was a descriptive analytic study with cross-sectional design. The sample of 192 Deutero Malay children consisted of boys and girls with an age range of 7-18 years old. Measurement of head height, length, and width was performed to calculate the differences of vertical, transverse, and horizontal cephalic index values by sex and age group, and the classification of the head shape based on the cephalic index values. Results: There was a difference of vertical, transverse, and horizontal by age group, with p = 0.000 (p < 0.05). The average of vertical, transverse, and horizontal cephalic index values was significantly higher in each age group. The average horizontal cephalic index was significantly higher in boys and girls. There was no significant difference in the head shape by the vertical and horizontal cephalic index in all age groups. The highest vertical cephalic index found was chamaecephalic (68%). The highest transverse cephalic index was tapeiocephalic (99%). The highest horizontal cephalic index was hyperbrachycephalic (80%). Conclusion: There is similar head shape of Deutero Malay children, although there may be increasing head size along with age. Keywords: Deutero Malay, head shape, cephalic index, transverse cephalic index, horizontal cephalic index, vertical cephalic index

The effect of the ethanolic extract of African leaves (Vernonia amygdalina Delile) on the corrosion rate and microstructure of stainless steel orthodontic wire Bahirrah, Siti; Ilyas, Syafruddin; Narmada , Ida Bagus; Primasari, Ameta; Lestari, Widya; Indra; Sofyanti, Ervina
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 4 (2025): December
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i4.p376-381

Background: Stainless steel orthodontic wire is commonly used in orthodontic treatment but is prone to corrosion; therefore, an inhibitor is required. Purpose: This study aimed to evaluate the effectiveness of the ethanolic extract of African leaves (Vernonia amygdalina Delile) as a corrosion inhibitor for stainless steel wires. Methods: A laboratory experiment with a pre- and post-test control group design was conducted. Samples included one negative control (artificial saliva), one positive control (chlorhexidine), and two treatment groups (ethanolic extract of African leaves at 3.125 mg/mL and 6.25 mg/mL), totaling 28 samples. Immersion lasted 7 days at 37°C. Corrosion rates were measured by weight loss, and microstructural changes were analyzed using a scanning electron microscope. Results: The highest average corrosion rate was observed in the chlorhexidine group (0.98 ± 0.58 mils per year [mpy]), whereas the lowest was in the 6.25 mg/mL extract group (−0.04 ± 0.80 mpy). Scanning electron microscope analysis revealed notable microstructural differences: the artificial saliva group showed long scratches scattered across the surface and localized round porosity; the chlorhexidine group exhibited rough scratches and widespread porosity; in contrast, the African leaves groups displayed minor scratches and a thin layer presumed to be protective. Conclusion: The ethanolic extract of African leaves considerably affects corrosion rates and microstructural changes in stainless steel orthodontic wires.

Co-Authors Adianti Adianti Agara, Dimas Alfarizi, Tengku Rizky Astrina, Ika Bong Chen Yong Bong Chen Yong Buaya, Erlin Kristiani Cynthia Cynthia DewaBrata, Gilang Dharli Syafriza DWI SURYANTO Ervina Sofyanti Essie Octiara Faradina, Ellin Feni Firdanigrum Heriandi Sutadi Hery Widijanto Hidayati, Rizka Ika Astrina Indra Juliani, Uta Lubis, Indri Madani, Sri Ayu Winda Michael Dany Minasari Minasari Nasution Minasari, Minasari Narmada , Ida Bagus R.Kintoko Rochadi Sandra Mega Sayuti Hasibuan Sembiring, Kartika Nabila Siregar, Arfi Luthfiyah Siti Bahirrah Sitompul, Safriani Syafruddin Ilyas Thantrira Porntaveetus Tyasingsih, Filia Dana Veronica Angelia Widjaja, Sry Suryani Yahwardiah Siregar Yahwardiyah Siregar Yendriwati Yendriwati Yendriwati Yumi Lindawati

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