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All Journal Dentika Dental Journal Majalah Kedokteran Gigi Indonesia Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Britain International of Humanities and Social Sciences (BIoHS) Journal Jurnal of Syiah Kuala Dentistry Society
Ameta Primasari
Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia
Religion Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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Effects of Sidamanik Black Tea on Salivary Flow Rate, Total Protein, and Albumin Levels in Young Adults: An in Vivo Study Ika Astrina; Ameta Primasari; Minasari; Filia Dana Tyaningsih
Dentika: Dental Journal Vol. 26 No. 2 (2023): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/dentika.v26i2.12717

Abstract

Saliva is crucial in maintaining oral health, performing functions such as antibacterial activity, buffering, and ensuring tooth integrity. In this regard, black tea, a medicinal plant with potential anti-cariogenic agents, has been suggested to exert preventive effects against caries. Therefore, this study aimed to determine effects of black tea consumption on salivary flow rate, pH, as well as total protein and albumin levels in young adults. A pre-experimental design was used with a one-group pretest-posttest approach. The samples selected were 18 healthy young adults aged 18-25 years, with DMF-T scores of 1-5. Salivary flow rate, total protein, and salivary albumin were measured using a digital scale after a 5-minute interval, the bicinchoninic acid assay method, and the bromocresol green method, respectively. The results showed a significant increase in salivary flow, a substantial decrease in albumin, and an insignificant elevation in total protein. In conclusion, black tea manifested the capability to prevent caries by enhancing salivary flow and total protein levels. Furthermore, the impact in inhibiting bacteria activity depended on the presence of salivary albumin.  
The Role of PAX9 and MSX1 Variants in Non-Syndromic Tooth Agenesis Finna; Ameta Primasari; Sry Suryani Widjaja; Thantrira Porntaveetus; Ervina Sofyanti
Dentika: Dental Journal Vol. 28 No. 2 (2025): Dentika: Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/dentika.v28i2.20787

Abstract

Non-syndromic tooth agenesis (NSTA) is the congenital absence of teeth without systemic involvement. Genetic polymorphisms in PAX9 and MSX1 are implicated in tooth development, but results vary across populations. Therefore, this study aims to explore the role of PAX9 c.-1031G>A and MSX1 671 T>C polymorphisms with NSTA in individuals from Medan. A total of 13 NSTA patients and 26 control were genotyped using PCR-restriction fragment length polymorphism (PCR-RFLP). The results showed that the AA genotype of PAX9 was absent in NSTA cases but found in 26.92% of controls. The GG genotype was reported to be more common in 38.46% of cases, while the G allele was prevalent in 69.23%. These trends suggested a potential association, but it was not statistically significant. In this study, all patients carried the TT genotype for MSX1. These results show a possible role of PAX9 polymorphisms in NSTA susceptibility and support the need for further studies in larger populations.   Keywords: Non-Syndromic, Tooth Agenesis, PAX9, MSX1
The Potential of Salivary Lysozyme Level Examination as Caries Biomarker: A Scoping Review Albertus Luis Sandro Samosir; Ameta Primasari
Dentika: Dental Journal Vol. 28 No. 2 (2025): Dentika: Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/dentika.v28i2.21593

Abstract

Salivary lysozyme is an antimicrobial enzyme naturally present in human saliva. It is widely recognized as a potential non-invasive biomarker for the early detection of dental caries, which remains a major global oral health concern. Lysozyme plays a significant role in oral immunity by degrading bacteria cell walls and maintaining microbial homeostasis. Therefore, this scoping review aimed to evaluate the potential of salivary lysozyme as a caries biomarker using PRISMA. The method was based on literature searches conducted in Scopus and PubMed databases between 2013 and 2023. The results showed that there were variations in lysozyme concentration according to caries status, where lower levels were often associated with a higher risk, but increased levels showed an active immune response to bacterial infection. Despite methodological differences among studies, there was association between salivary lysozyme concentration and caries severity, underscoring the diagnostic potential. Due to the non-invasive nature, ease of collection, and biochemical stability, salivary lysozyme examination could be applicable in both clinical and preventive settings. However, larger and standardized studies should be conducted in further studies to validate clinical reliability and diagnostic accuracy.
PENGARUH BERKUMUR LARUTAN EKSTRAK BONGGOL NANAS 20% TERHADAP LAJU ALIR, pH, DAN TOTAL PROTEIN SALIVA Samosir, Albertus Luis Sandro; Primasari, Ameta; Astrina, Ika; Yendriwati, Yendriwati
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 38, No 1 (2026): April 2026
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v38i1.64405

Abstract

Pendahuluan: Saliva memiliki peran penting dalam menjaga kesehatan rongga mulut yaitu menjaga pH optimal rongga mulut. Penurunan pH saliva yang menyebabkan demineralisasi email.  Tanaman nanas memiliki khasiat bagi rongga mulut, yaitu dapat meningkatkan sekresi saliva dan memiliki enzim bromelain yang banyak didapatkan pada bonggol nanas yang mampu menghambat bakteri sehingga dapat mencegah terjadinya karies. Tujuan penelitian ini adalah untuk menganalisis pengaruh berkumur larutan ekstrak bonggol nanas 20% terhadap laju alir, pH dan total protein saliva.Metode: Penelitian eksperimental kuasi dengan rancangan penelitian pretest-posttest design with control group design. Populasi pada penelitian ini adalah mahasiswa FKG USU berusia 18-22 tahun. Pengambilan sampel menggunakan purposive sampling dengan kriteria inklusi dan eksklusi. Sampel terbagi menjadi 2 kelompok dan berjumlah 10 subjek pada setiap kelompok perlakuan dan kontrol negatif.Saliva dikumpulkan sebelum dan sesudah berkumur larutan ekstrak bonggol nanas. Laju alir saliva diukur dengan cara menimbang saliva yang terkumpul dibagi per waktu, pH saliva diukur dengan menggunakan pH meter digital, total protein diukur dengan menggunakan PierceTM BCA Protein Assay Kit.Hasil: Penelitian menunjukkan berkumur larutan ekstrak bonggol nanas 20% memiliki pengaruh yang signifikan pada laju alir dan pH saliva (p < 0,05 ), sedangkan total protein memiliki pengaruh yang tidak signifikan.Kesimpulan: Penelitian ini bahwa berkumur larutan ekstrak bonggol nanas memiliki pengaruh yang baik terhadap laju alir dan pH saliva.
Co-Authors Adianti Adianti Agara, Dimas Albertus Luis Sandro Samosir Alfarizi, Tengku Rizky Astrina, Ika Bong Chen Yong Bong Chen Yong Buaya, Erlin Kristiani Cynthia Cynthia DewaBrata, Gilang Dharli Syafriza DWI SURYANTO Ervina Sofyanti Ervina Sofyanti Essie Octiara Faradina, Ellin Feni Firdanigrum Filia Dana Tyaningsih Finna Heriandi Sutadi Hidayati, Rizka Ika Astrina Ika Astrina Juliani, Uta Lubis, Indri Madani, Sri Ayu Winda Michael Dany Minasari Minasari Minasari Nasution Minasari, Minasari R.Kintoko Rochadi Samosir, Albertus Luis Sandro Sandra Mega Sayuti Hasibuan Sembiring, Kartika Nabila Siregar, Arfi Luthfiyah Sitompul, Safriani Sry Suryani Widjaja Thantrira Porntaveetus Thantrira Porntaveetus Tyasingsih, Filia Dana Veronica Angelia Widjaja, Sry Suryani Yahwardiah Siregar Yahwardiyah Siregar Yendriwati Yendriwati Yendriwati Yumi Lindawati