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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmiah Aplikasi Isotop Radiasi Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Iptek Tanaman Pangan AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Penelitian Tanaman Industri (Littri)
RAGAPADMI PURNAMANINGSIH
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology

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In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood Nurhayani, Siti; Megia, Rita; Purnamaningsih, Ragapadmi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11079

Abstract

A diversion of raw material from wood to bamboo is necessary. In vitro culture of bamboo can be used to provide a high number of seedling. The aim of this study was to increase the multiplication of a high quality Bambusa balcooa as a wood alternative material. Part of plants used was the sterile axillary shoot. The explants were planted on MS0 medium for 2 weeks and later on multiplication medium MS+0.3 mg/l BAP + 0.3mg/l TDZ. The shoots obtained were fragmented into clusters (3-5 shoots) used for the next multiplication stage using five different medium formulas: (1) MS0; MS containing: (2) 0.1 mg/l BAP, (3) 0.3 mg/l BAP, (4) 0.1 mg/l BAP + 0.1 mg/l TDZ and (5) 0.3 mg/l BAP + 0.1 mg/l TDZ. The results showed that MS medium containing 0.1 mg/l BAP + 0.1mg/l TDZ was the best medium for B. balcooa propagation. The shoots produced from aforementioned medium had a better quality compared to the other medium. Forty days after planting, the average number of shoots in this medium was 14.25. MS medium containing 0.3 mg/l BAP + 0.1 mg/l TDZ produced the highest number of shoot but in lower quality. Rooting medium containing 10 mg/l IBA + 5 mg/l NAA produced 9-16 root in 8 weeks. Vermicompost was more prevalent for the acclimatization of B. balcooa compared to compost. The use of B.balcooa resulted in in vitro propagation as a substitute alternative for wood is expected to save the environment from illegal logging.  
PENINGKATAN KERAGAMAN GENETIK MAWAR MINI MELALUI KULTURIN-VITRO DAN IRADIASI SINAR GAMMA Handayati, Wahyu; Darliah, Darliah; Mariska, I; Purnamaningsih, R
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.097 KB) | DOI: 10.14203/beritabiologi.v5i4.1120

Abstract

Mini rose (Rosa hybrida L.)is one of the favorite ornamental plants. To get a new appearance of this mini rose, two experiments were conducted at Cipanas and Bogor, from April 1999 to March 2000.In the first experiment, the treatment was the dosage of gamma ray irradiation, i.e. 0, 1, 2, 3, 4, 6, 8, 10 and 12 krad.In the second experiment, the treatment was the concentration of callus induced mediumi.e. 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5 and 1 mg/1) + chinetine (1, 3 and 5 mg/1).Randomized Block Design was used with 5 replications. Mini rose Romantica Meilandina (pink color) and Prince Meilandina (dark red color) was used as a source of plant material.The results showed that the irradiation dosage from 1 until 3 krad gave the best appearance compared with the other treatments.Considering the observance to plantlet in the same dosage, the color of the flower was changed from the natural color to white and red color.The combination of 0,5 mg/12,4- dichlorophenoxyacetic acid + 3 mg/1 chinetine was the best medium to the callus growth.
INDUKSI AKAR TUNAS KE LAPA SAWIT (Elaeis guineensis Jacq.) SECARA IN VITRO DAN EX VITRO / Root Induction of Oil Palm (Elaeis guineensis Jacq.) Using In Vitro and Ex Vitro Techniques Yunita, Rossa; MARISKA, IKA; PURNAMANINGSIH, RAGAPADMI; LESTARI, ENDANG GATI; UTAMI, SRI
Jurnal Penelitian Tanaman Industri Vol 22, No 1 (2016): Maret, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n1.2016.37-42

Abstract

Root induction is an important step in the process of propagation of plants through tissue culture techniques. For root induction commonly used plant growth regulator auxin, which has an important role in plant growth and development, especially at the stage of root initiation. The purpose of this study is to obtain appropriate methods for root induction of oil palm in vitro and ex vitro. The experiment used completely randomized factorial design, consisted of three main activities: (1) Induction of rooting on solid media, using combinations of NAA (2 and 4 mg/l) and IBA (1, 2, 3 and 4 mg/l) concentrations with 5 replications; (2) Induction of rooting the liquid media, using three concentrations of NAA (0, 3 and 6 mg/l), each treatment was replicated 5 times; (3) Induction of rooting ex vitro, using rootone F or IBA (20, 40 and 60 mg/l) with 5 replications. Results indicated that the best medium for in vitro root induction on solid media was MS + NAA 4 mg/l IBA + 4 mg/l, while for liquid media was MS + NAA 6 mg/l. Ex vitro rooting induction showed 60% success of acclimatization by soaking vitro shoots base in NAA solution 40 mg/l and 60 mg/l for 1 hour.Keywords: Elaeis guineensis Jacq., ex vitro, IBA, NAA
POLA INSERSI PARTENOKARPI, DEFH9-IAAM PADA GALUR TOMAT TRANSGENIK Pardal, S.J.; Slamet, Slamet; Purnamaningsih, R.; Lestari, E.G.
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.691

Abstract

The development of seedless tomato fruits will be more attractive to consumers and industry. Artificial parthenocarpy can be induced through genetic crossing, hormone application or genetic engineering. Development of parthenocarpic tomatos has been done by inserting parthenocarpy gene, DefH9-iaaM into tomato genome via Agrobacterium tumefaciens. Sixty putative transgenic tomato lines were produced, and three events (lines) have been selected as the best event, i.e. OvR1#14-4, OvM2#10-1, OvM2#6-2. These lines contained the DefH9-iaaM based on PCR test. This research aimed was to determine the insertion patern of DefH9-iaaM gene in the progeny of transgenic tomatos lines. Parent variety Oval and line Cl 6046 were used as control plants. Results indicated that tomatos line OvR1#14-4 was still contained the inserted DefH9-iaaM gene and followed the Mendelian pattern (3:1) based on molecular analyses and Chi-square test results, while the others were not identified. Line OvR1#14-4 was required to be further evaluated for phenotypic and genotypic analyses for the expression of their parthenocarpy.
INDUKSI MUTASI DAN SELEKSI IN VITRO TANAMAN GANDUM (Triticum aestivum L.) Sari, Laela; Purwito, Agus; Soepandi, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 2 (2016): December 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.087 KB) | DOI: 10.29122/jbbi.v3i2.36

Abstract

INDUCTION MUTATION AND SELECTION OF IN VITRO PLANT OF WHEAT (Triticum aestivum L.)The goal of this research was to produce wheat crop which is tolerant to lowland condition.Six varieties were used, Dewata, Selayar, Alibey, Oasis, Rabe and HP1744. This research consisted of 4 stages: production of the best callus on MS medium containing 3 g/L 2.4-D, induced mutation of embryogenic callus using EMS, in vitro selection of callus at temperature of 27–35°C, and callus regeneration. The best result for callus production was 76% for Dewata and 70% for Selayar varieties. Higher concentration of EMS and longer soaking time decreased the percentage of callus growth. LC50 for Dewata was 0.3% EMS at 30 minutes and that for Selayar was 0.1% EMS at 60 minutes. The higher the temperature was, the lower was the adaptation tolerant of the plants, and callus growth was inhibited. At the highest temperature (35°C) the callus did not grow at all.Keywords: Induced mutation, Triticum aestivum, EMS, in vitro selection, callusABSTRAKTujuan penelitian ini adalah untuk merakit tanaman gandum yang toleran pada dataran rendah. Varietas yang digunakan ada 6 yaitu Dewata, Selayar, Alibey, Oasis, Rabe dan HP-1744. Penelitian terdiri atas empat tahap yaitu induksi pembentukan kalus terbaik menggunakan media MS + 3 g/L 2,4-D (dipilih dua varietas yang terbaik), induksi mutasi kalus embriogenik menggunakan EMS, seleksi kalus in vitro pada suhu 27–35°C, dan regenerasi. Hasil induksi kalus terbaik terdapat pada varietas Dewata sebesar 76% dan Selayar sebesar 70%. Semakin tinggi konsentrasi EMS dan semakin lama waktu perendaman yang digunakan maka semakin menurun persentase pertumbuhan kalus. LC50 varietas Dewata adalah EMS 0,3% waktu 30 menit sedangkan LC50 varietas Selayar adalah EMS 0,1% waktu 60 menit.Semakin tinggi suhunya maka semakin berkurang toleran adaptasi tanaman tersebut, dan pertumbuhan kalus semakin sedikit. Bahkan pada suhu tertinggi yaitu suhu 35°C tidak ada pertumbuhan kalus sama sekali.Kata Kunci: Induksi mutasi, Triticum aestivum, EMS, seleksi in vitro, kalus
Multiplikasi Tunas dan Induksi Perakaran pada Ubi Kelapa (Dioscorea alata L.) dan Gembili (Dioscorea esculenta L.) Secara In Vitro Hutami, Sri; Purnamaningsih, Ragapadmi; Mariska, Ika; Diantina, Surya
Jurnal AgroBiogen Vol 10, No 2 (2014): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n2.2014.p53-60

Abstract

Dioscorea sp. (yam) is one of the minor tuber crops whichgrows wildly in the forest and only a few of its species arecultivated and used as main or secondary food.Conservation is needed to preserve plant genetic material.The objective of this research was to obtain methods ofplantlets propagation of D. alata L. and D. esculenta L.through in vitro culture. The research was conducted atTissue Culture Laboratory of ICABIOGRAD in 2012. Theresearch consisted of three stages. First, shoot emergence.In this experiment, young shoots were planted in MS basicmedium combined with benzyl adenine (BA) (0, 1, 3, and 5mg/l) and gibberelic acid (GA) (0 and 5 mg/l). Second, shootmultiplication. Shoots of Dioscorea which were planted inthe best medium of the first experiment were subcultured inMS medium combined with thidiazuron (0, 0.1, 0.5, 1, 2, and3 mg/l). Third, root initiation. Shoots of Dioscorea whichwere planted in the best medium of the second experimentwere subcultured in MS medium (½ MS and 1 MS)combined with indole-3-butyric acid (IBA) (0, 1, 3, and 5mg/l). Result of these experiments showed that shootemergence of D. alata L. and D. esculenta L. began at 2weeks after planting in MS medium. More plantlets of D.alata L. and D. esculenta L. were obtained by shootmultiplication in MS media. Root initiation of the Dioscoreabegan at 4 weeks after planting in MS media. The addition ofIBA (3–5 mg/l) on D. esculenta L. could not stimulate rootingbut led to the formation of callus at the base of the stembuds.
Pengaruh Sumber Karbon dan Kondisi Inkubasi terhadap Pertumbuhan Kultur In Vitro Purwoceng (Pimpinella pruatjan Molk.) Roostika, Ika; Purnamaningsih, Ragapadmi; Noviati, Arief V.
Jurnal AgroBiogen Vol 4, No 2 (2008): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n2.2008.p65-69

Abstract

Pruatjan (Pimpinella pruatjan Molk.) is an Indonesianmedicinal plant which is categorized as endangeredplant and included in Appendix I based on CITES. The invitro conservation techniques have been studied. However,the storage period was very short (4 months) when plantgrowth retardant and media dilution were applied. Besidethat, the residual effect of growth retardant was strongenough so that it needed more than 4 months for recovery.Thus, the use of certain carbon source may prolong thepreservation period with shorter time for recovery. Theobjective of the study was to know the effects of carbonsources (sucrose and mannitol) and culture conditions (cultureroom and growth chamber) to the growth of pruatjancultures. This application was hoped to prolong preservationperiod of pruatjan longer than 4 months and to cut therecovery period after presservation. The study was conductedat Tissue Culture Laboratory in Indonesian Center forAgricultural Biotechnology and Genetic Resources Researchand Development from August 2006 to July 2007. Theactivities included propagation of in vitro shoot grown invitro as explants source, preservation of in vitro shoots ofpruatjan, and regeneration of the cultures after preservation.The experiment was designed as factorial in RandomizedCompletely Block Design with 6 replications. The DKW basalmedia containing 1 ppm BA, 0.2 ppm thidiazuron, and 100ppm arginine were supplemented with mannitol or sucroseat the level of 1, 2, 3, 4, and 5%. The observed variables weretotal number of leaves, number of shoot, and number of wiltleaves. The result revealed that pruatjan cultures could bestored longer than 4 months. Generally, the effect ofmannitol or sucrose was more dominant than that of culturescondition. The mannitol (1-5%) strongly inhibited thegrowth of pruatjan cultures so that only a few culturessurvived at 7 months preservation period and needed about1 month for recovery. On the contrary, the effect of sucrose(at the same level) was better than mannitol. The 2.5%sucrose optimally inhibited pruatjan cultures. At that condition,the cultures could be stored for 10 months withoutmorphological changes so that they could recover spontaneously.
Induksi Kalus dan Optimasi Regenerasi Empat Varietas Padi melalui Kultur In Vitro Purnamaningsih, Ragapadmi
Jurnal AgroBiogen Vol 2, No 2 (2006): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n2.2006.p74-80

Abstract

A study was conducted at the Tissue Culture Laboratory of ICABIOGRAD, Bogor, to obtain an optimum medium formulation for calli regenerations of for rice varities (Ciherang, Cisadane, IR64, and T-309). The research activities were done in five steps, i.e., callus induction, callus regeneration, shoot multiplication, root formation, and plant acclimatization. The type of explants used in the study was embriozygotic explants. Five media formulations were used for the callus induction, while four media formulations were used for the callus regeneration. The results showed that the best medium formulation for induction of callus formation was MS + 2,4-D 2 mg/l + casein hidrolisat 3 mg/l, while the best medium formulation for callus regeneration was MS + BA 3 mg/l + thidiazuron 0,1 mg/l.
Perkembangan Pemuliaan Gandum di Indonesia Nur, Amin; Azrai, Muh.; Subagio, Herman; Soeranto, Soeranto; Ragapadmi, Ragapadmi; Sustiprajitno, Sustiprajitno; Trikoesoemaningtyas, Trikoesoemaningtyas
Iptek Tanaman Pangan Vol 8, No 2 (2013): Desember 2013
Publisher : Puslitbang Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

As a tropical country, Indonesia certainly is not the most suitable place for producing wheat. Wheat flour consumption however, had increased tremendously during the last three decades, that import of wheat grain in 2012 reached 7.2 million ton. Producing wheat had been attempted since 1990’s, utilizing lands at high elevation with drier climate. However, crops competition, especially with the high land vegetables, had put wheat cropping become prohibitive. As early as in 1880, G Wallace experimented of growing wheat in the high land of Timor island. However the crop was never economically established. Selection for genotypes and varieties of wheat for the tropical area had been attempted since 1980’s, and adaptable varieties to the high land had been released, but farmers did not adopt the crop on their cropping system. Breeding research to develop tropical wheat varieties was revived in 2009. By using the modern breeding techniques, including biotechnology, cellular somatic mutation, as well as applying the conventional breeding techniques, varieties adaptable to the lowland tropics are expected to be identified.
Analisis Fenotipik Progeni Tiga Galur Tomat Transgenik Partenokarpi di Fasilitas Uji Terbatas (Phenotypic Analysis on Progenies of Three Transgenic Parthenocarpy Tomato Lines in Biosafety Containment) Pardal, Saptowo Jumali; Purnamaningsih, Ragapadmi; Lestari, Endang Gati; Slamet, nFN
Jurnal Hortikultura Vol 26, No 1 (2016): Juni 2016
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v26n1.2016.p21-30

Abstract

Kebutuhan masyarakat akan buah tomat konsumsi dan untuk industri cenderung kian meningkat setiap tahunnya, sedangkan produksi tomat masih rendah. Hingga kini, produksi tomat nasional masih sangat rendah,yaitu 992.780 ton sehingga belum mencukupi kebutuhan pasar yang mencapai 1.230.000 ton. Beberapa upaya telah dilakukan pemerintah untuk meningkatkan produksi tomat, tetapi masih menemui beberapa masalah di lapangan. Perakitan varietas unggul tomat produktivitas tinggi dan tanpa biji (seedless) sangatlah diharapkan guna memenuhi kebutuhan masyarakat dan industri. Partenokarpi merupakan fenomena terjadinya pembentukan buah tanpa melalui proses penyerbukan dan atau pembuahan. Teknologi partenokarpi dapat digunakan untuk meningkatkan produksi tomat melalui peningkatan pembentukan buah (fruits setting) dan buah tanpa biji. Perakitan galur tomat partenokarpi melalui rekayasa genetik telah dilakukan di Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian (BB Biogen). Enam puluh galur (event) tomat transgenik T0 yang membawa gen DefH9-iaaM telah dihasilkan dan tiga galur di antaranya, yaitu OvR#14-4, OvM2#10-1, dan OvM2#6-2 telah terpilih sebagai galur terbaik berdasarkan karakter partenokarpinya. Galur tomat transgenik ini selanjutnya dievaluasi lebih lanjut secara molekuler dan fenotipik di rumah kaca dan rumah kasa fasilitas uji terbatas (FUT). Hasil analisis molekuler menunjukkan bahwa semua galur tomat transgenik yang diuji masih membawa gen DefH9-iaaM. Hasil evaluasi awal terhadap tiga galur tomat transgenik secara fenotipik juga menunjukkan sifat partenokarpi, yaitu meningkatnya jumlah buah, berat buah, dan berkurangnya jumlah biji (seedless). Analisis fenotipik lebih lanjut terhadap progeni ketiga galur tersebut pada percobaan ini menunjukkan adanya ekspresi fenotipik dari gen DefH9-iaaM, di mana galur OvR#14-4 memiliki ekspresi fenotipik partenokarpi lebih baik daripada galur OvM2#10-1 dan OvM2#6-2.KeywordsTomat; Rekayasa genetik; Gen partenokarpi; Galur transgenik; Analisis fenotipikAbstractThe demand of tomato fruits for daily consumption and industry materials tend to increase annually, while the tomato production is still low. Up till now, the national tomato production was still low (992,780 ton) so that it’s not enough to fulfill the market demand (1,230,000 ton). Many effort have been conducted by the government in order to increase the tomato production, but there were still many contrains in the field. The development of elite tomato cultivars is very urgent in order to suffice the public and industrial demand. Parthenocarpy is a phenomenon that fruits can be produced without pollination and fertilization. Parthenocarpy technology can be used to increase tomato productivity through increasing the fruit setting and seedless fruits. The development of transgenic parthenocarpy tomato cultivars was conducted through genetic engineering at Central Research for Biotechnology and Genetic Resources Research and Development (ICABIOGRAD). Sixty transgenic T0 tomato lines which contain DefH9-iaaM gene were produced. Those transgenic tomato lines were then further evaluated by molecular and phenotypic in biosafety containment. Molecular analysis showed that all tomato lines were still contained DefH9-iaaM gene. The phenotypic analysis showed parthenocarpy phenotypic, such as increasing fruits number, fruits weight, and decreasing seeds number. Further phenotypic analysis on progeny of those lines in this experiments showed the phenotypic characters of the parthenocarpy gene. Line OvR1#14-4 had better parthenocarpy characters than that of lines OvM2#10-1 and OvM2#6-2.
Co-Authors A G Wattimena Agus Purwito Ashrina, Misky Azrai, Muh. DANNY LAURENT Darliah Darliah Deden Sukmadjaja Diantina, Surya Didy Soepandi Didy Soepandi, Didy Didy Sopandie E.G. Lestari E.G. Lestari E.G. Lestari Endang G Lestari Endang G Lestari Endang Gati Lestari Endang Gati Lestari ENDANG GATI LESTARI ENDANG GATI LESTARI ENDANG GATI LESTARI Enny Sudarmonowati Enny Sudarmonowati GA Wattimena Hutami, Sri I Mariska I Roostika I. Darwati I. Darwati I. DARWATI I. Mariska I. Roostika I. Roostika I. ROOSTIKA Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Roostika Ika Roostika Ika Rostika Ika Rostika Ireng Darwati IRENG DARWATI Laela Sari laela Sari, laela LESTARI, ENDANG GATI Lestari, Endang Gati Lizawati . Mariska, I Mariska, Ika MARISKA, IKA Muhamad Syukur Muhammad Syukur N Khumaida N Khumaida Nesti Fronika Sianipar NESTI FRONIKA SIANIPAR NESTI FRONIKA SIANIPAR Noviati, Arief V. Nur, Amin Nurhayani, Siti Pardal, S.J. Pardal, Saptowo Jumali Rita Megia RITA MEGIA ROHIM FIRDAUS Rohim Firdaus ROHIM FIRDAUS Roostika, Ika Rosa Yunita Rosa Yunita Rosa Yunita Rosaria Rosiana Rosaria Rosiana, Rosaria Rossa Yunita ROSSA YUNITA S.J. Pardal Sari, Laela Siti Nurhayani Slamet . Slamet Slamet Slamet, nFN Soeranto, Soeranto Sri Hutami Sri Hutami Sri Hutami Subagio, Herman Sudarmonowati, Enny Sustiprajitno, Sustiprajitno Syarifah Iis Aisyah Tri P. Priyatno Trias Novita Trikoesoemaningtyas UTAMI, SRI Wahyu Handayati Wahyu Handayati Y Supriati Y Supriati Yati Supriati, Yati Yunita, Rossa